AlGaN/GaN-based biosensor for label-free detection of biological activity
In this work, detection of live cell biological activity has been confirmed for label-free AlGaN/GaN based biosensors. Live cells were seeded in solution onto the exposed gate region of packaged AlGaN/GaN field effect transistor (FET) devices and subsequently subjected to various chemical changes in...
Gespeichert in:
| Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2013-02, Vol.177, p.577-582 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 582 |
|---|---|
| container_issue | |
| container_start_page | 577 |
| container_title | Sensors and actuators. B, Chemical |
| container_volume | 177 |
| creator | Podolska, A. Hool, L.C. Pfleger, K.D.G. Mishra, U.K. Parish, G. Nener, B.D. |
| description | In this work, detection of live cell biological activity has been confirmed for label-free AlGaN/GaN based biosensors. Live cells were seeded in solution onto the exposed gate region of packaged AlGaN/GaN field effect transistor (FET) devices and subsequently subjected to various chemical changes in the solution. Cell depolarisation with KCl was used to induce biological signals. Significant optimisation of experimental parameters was performed, including environmental conditions and cell seeding concentration. Selectivity towards biological activity was improved by buffering with 30mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). Reproducible calcium concentration-dependent responses were observed with saturation at the normal physiological level of around 2.5mM. Cells in a calcium-free solution were pre-treated in separate experiments with calcium ion channel inhibitors Mibefradil, Nisoldipine and HC-030031, and the calcium channel activator S-BayK(−)8644, before calcium dosing. The resulting responses were compared to depolarisation-induced calcium intake and demonstrated physiologically relevant behaviour of calcium ion channels. |
| doi_str_mv | 10.1016/j.snb.2012.11.086 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1315691583</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0925400512012828</els_id><sourcerecordid>1315691583</sourcerecordid><originalsourceid>FETCH-LOGICAL-c354t-464b4cd2173f8bae703029b074f1fe56972752a887fae1ffc9be6eda91e5c2b13</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMouH78AE_26KXdmaZtWjzJouuC6EE9hySdSJbarEl3Yf-9Wdazh2FgeN4X5mHsBqFAwGa-LuKoixKwLBALaJsTNsNW8JyDEKdsBl1Z5xVAfc4uYlwDQMUbmLHVw7BUr_M0uVaR-kw7H2mMPmQ2zaA0DbkNRFlPE5nJ-THz9kAN_ssZNWQqHXdu2l-xM6uGSNd_-5J9Pj1-LJ7zl7flavHwkhteV1NeNZWuTF-i4LbVigRwKDsNorJoqW46UYq6VG0rrCK01nSaGupVh1SbUiO_ZHfH3k3wP1uKk_x20dAwqJH8NkrkmFqwbnlC8Yia4GMMZOUmuG8V9hJBHrTJtUza5EGbRJRJW8rcHjNWeam-govy8z0BNQBiU3UH4v5IUPpy5yjIaByNhnoXkiHZe_dP_y8ben4x</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1315691583</pqid></control><display><type>article</type><title>AlGaN/GaN-based biosensor for label-free detection of biological activity</title><source>Elsevier ScienceDirect Journals</source><creator>Podolska, A. ; Hool, L.C. ; Pfleger, K.D.G. ; Mishra, U.K. ; Parish, G. ; Nener, B.D.</creator><creatorcontrib>Podolska, A. ; Hool, L.C. ; Pfleger, K.D.G. ; Mishra, U.K. ; Parish, G. ; Nener, B.D.</creatorcontrib><description>In this work, detection of live cell biological activity has been confirmed for label-free AlGaN/GaN based biosensors. Live cells were seeded in solution onto the exposed gate region of packaged AlGaN/GaN field effect transistor (FET) devices and subsequently subjected to various chemical changes in the solution. Cell depolarisation with KCl was used to induce biological signals. Significant optimisation of experimental parameters was performed, including environmental conditions and cell seeding concentration. Selectivity towards biological activity was improved by buffering with 30mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). Reproducible calcium concentration-dependent responses were observed with saturation at the normal physiological level of around 2.5mM. Cells in a calcium-free solution were pre-treated in separate experiments with calcium ion channel inhibitors Mibefradil, Nisoldipine and HC-030031, and the calcium channel activator S-BayK(−)8644, before calcium dosing. The resulting responses were compared to depolarisation-induced calcium intake and demonstrated physiologically relevant behaviour of calcium ion channels.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2012.11.086</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>AlGaN/GaN ; bioactive properties ; Biosensor ; biosensors ; calcium ; Calcium activated ion channels ; calcium channels ; environmental factors ; Label-free pharmacology ; Live cells ; packaging ; potassium chloride</subject><ispartof>Sensors and actuators. B, Chemical, 2013-02, Vol.177, p.577-582</ispartof><rights>2012 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-464b4cd2173f8bae703029b074f1fe56972752a887fae1ffc9be6eda91e5c2b13</citedby><cites>FETCH-LOGICAL-c354t-464b4cd2173f8bae703029b074f1fe56972752a887fae1ffc9be6eda91e5c2b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0925400512012828$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Podolska, A.</creatorcontrib><creatorcontrib>Hool, L.C.</creatorcontrib><creatorcontrib>Pfleger, K.D.G.</creatorcontrib><creatorcontrib>Mishra, U.K.</creatorcontrib><creatorcontrib>Parish, G.</creatorcontrib><creatorcontrib>Nener, B.D.</creatorcontrib><title>AlGaN/GaN-based biosensor for label-free detection of biological activity</title><title>Sensors and actuators. B, Chemical</title><description>In this work, detection of live cell biological activity has been confirmed for label-free AlGaN/GaN based biosensors. Live cells were seeded in solution onto the exposed gate region of packaged AlGaN/GaN field effect transistor (FET) devices and subsequently subjected to various chemical changes in the solution. Cell depolarisation with KCl was used to induce biological signals. Significant optimisation of experimental parameters was performed, including environmental conditions and cell seeding concentration. Selectivity towards biological activity was improved by buffering with 30mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). Reproducible calcium concentration-dependent responses were observed with saturation at the normal physiological level of around 2.5mM. Cells in a calcium-free solution were pre-treated in separate experiments with calcium ion channel inhibitors Mibefradil, Nisoldipine and HC-030031, and the calcium channel activator S-BayK(−)8644, before calcium dosing. The resulting responses were compared to depolarisation-induced calcium intake and demonstrated physiologically relevant behaviour of calcium ion channels.</description><subject>AlGaN/GaN</subject><subject>bioactive properties</subject><subject>Biosensor</subject><subject>biosensors</subject><subject>calcium</subject><subject>Calcium activated ion channels</subject><subject>calcium channels</subject><subject>environmental factors</subject><subject>Label-free pharmacology</subject><subject>Live cells</subject><subject>packaging</subject><subject>potassium chloride</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMouH78AE_26KXdmaZtWjzJouuC6EE9hySdSJbarEl3Yf-9Wdazh2FgeN4X5mHsBqFAwGa-LuKoixKwLBALaJsTNsNW8JyDEKdsBl1Z5xVAfc4uYlwDQMUbmLHVw7BUr_M0uVaR-kw7H2mMPmQ2zaA0DbkNRFlPE5nJ-THz9kAN_ssZNWQqHXdu2l-xM6uGSNd_-5J9Pj1-LJ7zl7flavHwkhteV1NeNZWuTF-i4LbVigRwKDsNorJoqW46UYq6VG0rrCK01nSaGupVh1SbUiO_ZHfH3k3wP1uKk_x20dAwqJH8NkrkmFqwbnlC8Yia4GMMZOUmuG8V9hJBHrTJtUza5EGbRJRJW8rcHjNWeam-govy8z0BNQBiU3UH4v5IUPpy5yjIaByNhnoXkiHZe_dP_y8ben4x</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Podolska, A.</creator><creator>Hool, L.C.</creator><creator>Pfleger, K.D.G.</creator><creator>Mishra, U.K.</creator><creator>Parish, G.</creator><creator>Nener, B.D.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20130201</creationdate><title>AlGaN/GaN-based biosensor for label-free detection of biological activity</title><author>Podolska, A. ; Hool, L.C. ; Pfleger, K.D.G. ; Mishra, U.K. ; Parish, G. ; Nener, B.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-464b4cd2173f8bae703029b074f1fe56972752a887fae1ffc9be6eda91e5c2b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>AlGaN/GaN</topic><topic>bioactive properties</topic><topic>Biosensor</topic><topic>biosensors</topic><topic>calcium</topic><topic>Calcium activated ion channels</topic><topic>calcium channels</topic><topic>environmental factors</topic><topic>Label-free pharmacology</topic><topic>Live cells</topic><topic>packaging</topic><topic>potassium chloride</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Podolska, A.</creatorcontrib><creatorcontrib>Hool, L.C.</creatorcontrib><creatorcontrib>Pfleger, K.D.G.</creatorcontrib><creatorcontrib>Mishra, U.K.</creatorcontrib><creatorcontrib>Parish, G.</creatorcontrib><creatorcontrib>Nener, B.D.</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Podolska, A.</au><au>Hool, L.C.</au><au>Pfleger, K.D.G.</au><au>Mishra, U.K.</au><au>Parish, G.</au><au>Nener, B.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AlGaN/GaN-based biosensor for label-free detection of biological activity</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2013-02-01</date><risdate>2013</risdate><volume>177</volume><spage>577</spage><epage>582</epage><pages>577-582</pages><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>In this work, detection of live cell biological activity has been confirmed for label-free AlGaN/GaN based biosensors. Live cells were seeded in solution onto the exposed gate region of packaged AlGaN/GaN field effect transistor (FET) devices and subsequently subjected to various chemical changes in the solution. Cell depolarisation with KCl was used to induce biological signals. Significant optimisation of experimental parameters was performed, including environmental conditions and cell seeding concentration. Selectivity towards biological activity was improved by buffering with 30mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). Reproducible calcium concentration-dependent responses were observed with saturation at the normal physiological level of around 2.5mM. Cells in a calcium-free solution were pre-treated in separate experiments with calcium ion channel inhibitors Mibefradil, Nisoldipine and HC-030031, and the calcium channel activator S-BayK(−)8644, before calcium dosing. The resulting responses were compared to depolarisation-induced calcium intake and demonstrated physiologically relevant behaviour of calcium ion channels.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2012.11.086</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0925-4005 |
| ispartof | Sensors and actuators. B, Chemical, 2013-02, Vol.177, p.577-582 |
| issn | 0925-4005 1873-3077 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1315691583 |
| source | Elsevier ScienceDirect Journals |
| subjects | AlGaN/GaN bioactive properties Biosensor biosensors calcium Calcium activated ion channels calcium channels environmental factors Label-free pharmacology Live cells packaging potassium chloride |
| title | AlGaN/GaN-based biosensor for label-free detection of biological activity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T03%3A12%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=AlGaN/GaN-based%20biosensor%20for%20label-free%20detection%20of%20biological%20activity&rft.jtitle=Sensors%20and%20actuators.%20B,%20Chemical&rft.au=Podolska,%20A.&rft.date=2013-02-01&rft.volume=177&rft.spage=577&rft.epage=582&rft.pages=577-582&rft.issn=0925-4005&rft.eissn=1873-3077&rft_id=info:doi/10.1016/j.snb.2012.11.086&rft_dat=%3Cproquest_cross%3E1315691583%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1315691583&rft_id=info:pmid/&rft_els_id=S0925400512012828&rfr_iscdi=true |