Role of surface charge in cytotoxicity of charged manganese ferrite nanoparticles towards macrophages
Amphiphilic surfactants have been used to disperse magnetic nanoparticles in biological media, because they exhibit a dual hydrophobic/hydrophilic affinity that facilitates the formation of a nanoemulsion, within which nanoparticle surfaces can be modified to achieve different physicochemical proper...
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| Veröffentlicht in: | Nanotechnology 2012-12, Vol.23 (50), p.505702-505702 |
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| container_title | Nanotechnology |
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| creator | Yang, Seung-Hyun Heo, Dan Park, Jinsung Na, Sungsoo Suh, Jin-Suck Haam, Seungjoo Wook Park, Sahng Huh, Yong-Min Yang, Jaemoon |
| description | Amphiphilic surfactants have been used to disperse magnetic nanoparticles in biological media, because they exhibit a dual hydrophobic/hydrophilic affinity that facilitates the formation of a nanoemulsion, within which nanoparticle surfaces can be modified to achieve different physicochemical properties. For the investigation of the interactions of cells with charged magnetic nanoparticles in a biological medium, we selected the nanoemulsion method to prepare water-soluble magnetic nanoparticles using amphiphilic surfactant (polysorbate 80). The hydroxyl groups of polysorbate 80 were modified to carboxyl or amine groups. The chemical structures of carboxylated and aminated polysorbate 80 were confirmed, and water-soluble manganese ferrite nanoparticles (MFNPs) were synthesized with three types of polysorbate 80. Colloidal size, morphology, monodispersity, solubility and T2 relaxivity were found to be similar between the three types of MFNP. However, cationic MFNPs exhibited greater cytotoxicity in macrophages (RAW264.7 cells) and lower cellular membrane effective stiffness than anionic and non-ionic MFNPs. Moreover, cationic MFNPs exhibited large uptake efficiency for RAW264.7 cells compared with anionic or non-ionic MFNPs under the same conditions. Therefore, we propose that surface charge should be a key consideration factor in the design of magnetic nanoparticles for theragnostic applications. |
| doi_str_mv | 10.1088/0957-4484/23/50/505702 |
| format | Article |
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For the investigation of the interactions of cells with charged magnetic nanoparticles in a biological medium, we selected the nanoemulsion method to prepare water-soluble magnetic nanoparticles using amphiphilic surfactant (polysorbate 80). The hydroxyl groups of polysorbate 80 were modified to carboxyl or amine groups. The chemical structures of carboxylated and aminated polysorbate 80 were confirmed, and water-soluble manganese ferrite nanoparticles (MFNPs) were synthesized with three types of polysorbate 80. Colloidal size, morphology, monodispersity, solubility and T2 relaxivity were found to be similar between the three types of MFNP. However, cationic MFNPs exhibited greater cytotoxicity in macrophages (RAW264.7 cells) and lower cellular membrane effective stiffness than anionic and non-ionic MFNPs. Moreover, cationic MFNPs exhibited large uptake efficiency for RAW264.7 cells compared with anionic or non-ionic MFNPs under the same conditions. Therefore, we propose that surface charge should be a key consideration factor in the design of magnetic nanoparticles for theragnostic applications.</description><identifier>ISSN: 0957-4484</identifier><identifier>EISSN: 1361-6528</identifier><identifier>DOI: 10.1088/0957-4484/23/50/505702</identifier><identifier>PMID: 23164999</identifier><identifier>CODEN: NNOTER</identifier><language>eng</language><publisher>England: IOP Publishing</publisher><subject>Animals ; Biological ; Cationic ; Cell Line ; Cell Survival - drug effects ; Ferric Compounds - chemistry ; Ferric Compounds - toxicity ; Ferrite ; Hydrophobic and Hydrophilic Interactions ; Ions - chemistry ; Ions - toxicity ; Macrophages ; Macrophages - cytology ; Macrophages - drug effects ; Manganese Compounds - chemistry ; Mice ; Nanomaterials ; Nanoparticles ; Nanoparticles - chemistry ; Nanoparticles - toxicity ; Nanoparticles - ultrastructure ; Nanostructure ; Particle Size ; Polysorbates - chemistry ; Solubility ; Surface-Active Agents - chemistry ; Surfactants</subject><ispartof>Nanotechnology, 2012-12, Vol.23 (50), p.505702-505702</ispartof><rights>2012 IOP Publishing Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-900af1931e6e4c5a529ab1aa00f64f3a4c9b5e4d10201ad93ae16659592ab51e3</citedby><cites>FETCH-LOGICAL-c453t-900af1931e6e4c5a529ab1aa00f64f3a4c9b5e4d10201ad93ae16659592ab51e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://iopscience.iop.org/article/10.1088/0957-4484/23/50/505702/pdf$$EPDF$$P50$$Giop$$H</linktopdf><link.rule.ids>315,781,785,27929,27930,53851,53898</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23164999$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Seung-Hyun</creatorcontrib><creatorcontrib>Heo, Dan</creatorcontrib><creatorcontrib>Park, Jinsung</creatorcontrib><creatorcontrib>Na, Sungsoo</creatorcontrib><creatorcontrib>Suh, Jin-Suck</creatorcontrib><creatorcontrib>Haam, Seungjoo</creatorcontrib><creatorcontrib>Wook Park, Sahng</creatorcontrib><creatorcontrib>Huh, Yong-Min</creatorcontrib><creatorcontrib>Yang, Jaemoon</creatorcontrib><title>Role of surface charge in cytotoxicity of charged manganese ferrite nanoparticles towards macrophages</title><title>Nanotechnology</title><addtitle>Nano</addtitle><addtitle>Nanotechnology</addtitle><description>Amphiphilic surfactants have been used to disperse magnetic nanoparticles in biological media, because they exhibit a dual hydrophobic/hydrophilic affinity that facilitates the formation of a nanoemulsion, within which nanoparticle surfaces can be modified to achieve different physicochemical properties. For the investigation of the interactions of cells with charged magnetic nanoparticles in a biological medium, we selected the nanoemulsion method to prepare water-soluble magnetic nanoparticles using amphiphilic surfactant (polysorbate 80). The hydroxyl groups of polysorbate 80 were modified to carboxyl or amine groups. The chemical structures of carboxylated and aminated polysorbate 80 were confirmed, and water-soluble manganese ferrite nanoparticles (MFNPs) were synthesized with three types of polysorbate 80. Colloidal size, morphology, monodispersity, solubility and T2 relaxivity were found to be similar between the three types of MFNP. However, cationic MFNPs exhibited greater cytotoxicity in macrophages (RAW264.7 cells) and lower cellular membrane effective stiffness than anionic and non-ionic MFNPs. Moreover, cationic MFNPs exhibited large uptake efficiency for RAW264.7 cells compared with anionic or non-ionic MFNPs under the same conditions. Therefore, we propose that surface charge should be a key consideration factor in the design of magnetic nanoparticles for theragnostic applications.</description><subject>Animals</subject><subject>Biological</subject><subject>Cationic</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Ferric Compounds - chemistry</subject><subject>Ferric Compounds - toxicity</subject><subject>Ferrite</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Ions - chemistry</subject><subject>Ions - toxicity</subject><subject>Macrophages</subject><subject>Macrophages - cytology</subject><subject>Macrophages - drug effects</subject><subject>Manganese Compounds - chemistry</subject><subject>Mice</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - toxicity</subject><subject>Nanoparticles - ultrastructure</subject><subject>Nanostructure</subject><subject>Particle Size</subject><subject>Polysorbates - chemistry</subject><subject>Solubility</subject><subject>Surface-Active Agents - chemistry</subject><subject>Surfactants</subject><issn>0957-4484</issn><issn>1361-6528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtLJDEQgMOyyzq6-xckRy_tpPKycxTZhzAgiHsONenqsaWn0ybduPPvzdDqVQjUIV-9vmLsHMQliLpeC2euKq1rvZZqbUR55krIL2wFykJljay_stUHdMJOc34SAqCW8J2dSAVWO-dWjO5jTzy2PM-pxUA8PGLaEe8GHg5TnOL_LnTT4UgsPw3f47DDgTLxllLqJuIDDnHENHWhp8yn-IKpyYULKY6PuKP8g31rsc_08y2esX-_fz3c_K02d39ub643VdBGTZUTAltwCsiSDgaNdLgFRCFaq1uFOritId2AkAKwcQoJrDXOOIlbA6TO2MVSd0zxeaY8-X2XA_V9mTfO2YMCY4sqZz9HpYTisgZZULugZZ-cE7V-TN0e08GD8Mdr-KNofxTtpfJG-OUaJfH8rce83VPzkfauvwByAbo4-qc4p6HY-azqK_1blOc</recordid><startdate>20121221</startdate><enddate>20121221</enddate><creator>Yang, Seung-Hyun</creator><creator>Heo, Dan</creator><creator>Park, Jinsung</creator><creator>Na, Sungsoo</creator><creator>Suh, Jin-Suck</creator><creator>Haam, Seungjoo</creator><creator>Wook Park, Sahng</creator><creator>Huh, Yong-Min</creator><creator>Yang, Jaemoon</creator><general>IOP Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20121221</creationdate><title>Role of surface charge in cytotoxicity of charged manganese ferrite nanoparticles towards macrophages</title><author>Yang, Seung-Hyun ; Heo, Dan ; Park, Jinsung ; Na, Sungsoo ; Suh, Jin-Suck ; Haam, Seungjoo ; Wook Park, Sahng ; Huh, Yong-Min ; Yang, Jaemoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-900af1931e6e4c5a529ab1aa00f64f3a4c9b5e4d10201ad93ae16659592ab51e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Biological</topic><topic>Cationic</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Ferric Compounds - chemistry</topic><topic>Ferric Compounds - toxicity</topic><topic>Ferrite</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Ions - chemistry</topic><topic>Ions - toxicity</topic><topic>Macrophages</topic><topic>Macrophages - cytology</topic><topic>Macrophages - drug effects</topic><topic>Manganese Compounds - chemistry</topic><topic>Mice</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - toxicity</topic><topic>Nanoparticles - ultrastructure</topic><topic>Nanostructure</topic><topic>Particle Size</topic><topic>Polysorbates - chemistry</topic><topic>Solubility</topic><topic>Surface-Active Agents - chemistry</topic><topic>Surfactants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Seung-Hyun</creatorcontrib><creatorcontrib>Heo, Dan</creatorcontrib><creatorcontrib>Park, Jinsung</creatorcontrib><creatorcontrib>Na, Sungsoo</creatorcontrib><creatorcontrib>Suh, Jin-Suck</creatorcontrib><creatorcontrib>Haam, Seungjoo</creatorcontrib><creatorcontrib>Wook Park, Sahng</creatorcontrib><creatorcontrib>Huh, Yong-Min</creatorcontrib><creatorcontrib>Yang, Jaemoon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Nanotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Seung-Hyun</au><au>Heo, Dan</au><au>Park, Jinsung</au><au>Na, Sungsoo</au><au>Suh, Jin-Suck</au><au>Haam, Seungjoo</au><au>Wook Park, Sahng</au><au>Huh, Yong-Min</au><au>Yang, Jaemoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of surface charge in cytotoxicity of charged manganese ferrite nanoparticles towards macrophages</atitle><jtitle>Nanotechnology</jtitle><stitle>Nano</stitle><addtitle>Nanotechnology</addtitle><date>2012-12-21</date><risdate>2012</risdate><volume>23</volume><issue>50</issue><spage>505702</spage><epage>505702</epage><pages>505702-505702</pages><issn>0957-4484</issn><eissn>1361-6528</eissn><coden>NNOTER</coden><abstract>Amphiphilic surfactants have been used to disperse magnetic nanoparticles in biological media, because they exhibit a dual hydrophobic/hydrophilic affinity that facilitates the formation of a nanoemulsion, within which nanoparticle surfaces can be modified to achieve different physicochemical properties. For the investigation of the interactions of cells with charged magnetic nanoparticles in a biological medium, we selected the nanoemulsion method to prepare water-soluble magnetic nanoparticles using amphiphilic surfactant (polysorbate 80). The hydroxyl groups of polysorbate 80 were modified to carboxyl or amine groups. The chemical structures of carboxylated and aminated polysorbate 80 were confirmed, and water-soluble manganese ferrite nanoparticles (MFNPs) were synthesized with three types of polysorbate 80. Colloidal size, morphology, monodispersity, solubility and T2 relaxivity were found to be similar between the three types of MFNP. However, cationic MFNPs exhibited greater cytotoxicity in macrophages (RAW264.7 cells) and lower cellular membrane effective stiffness than anionic and non-ionic MFNPs. Moreover, cationic MFNPs exhibited large uptake efficiency for RAW264.7 cells compared with anionic or non-ionic MFNPs under the same conditions. Therefore, we propose that surface charge should be a key consideration factor in the design of magnetic nanoparticles for theragnostic applications.</abstract><cop>England</cop><pub>IOP Publishing</pub><pmid>23164999</pmid><doi>10.1088/0957-4484/23/50/505702</doi><tpages>8</tpages></addata></record> |
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| ispartof | Nanotechnology, 2012-12, Vol.23 (50), p.505702-505702 |
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| subjects | Animals Biological Cationic Cell Line Cell Survival - drug effects Ferric Compounds - chemistry Ferric Compounds - toxicity Ferrite Hydrophobic and Hydrophilic Interactions Ions - chemistry Ions - toxicity Macrophages Macrophages - cytology Macrophages - drug effects Manganese Compounds - chemistry Mice Nanomaterials Nanoparticles Nanoparticles - chemistry Nanoparticles - toxicity Nanoparticles - ultrastructure Nanostructure Particle Size Polysorbates - chemistry Solubility Surface-Active Agents - chemistry Surfactants |
| title | Role of surface charge in cytotoxicity of charged manganese ferrite nanoparticles towards macrophages |
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