Improved PCR performance using mutant Tpa-S DNA polymerases from the hyperthermophilic archaeon Thermococcus pacificus

► Thermococcus pacificus-S N213D/K501R (Tpa-S N213D/K501R) gene contained open reading frame that encoded 840 amino acid residues. ► The molecular mass of Tpa-S N213D/K501R DNA polymerase was about 97kDa. ► Tpa-S N213D/K501R DNA polymerase could amplify 12kb target template at 30s extension time per...

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Veröffentlicht in:	Journal of biotechnology 2013-03, Vol.164 (2), p.363-370
Hauptverfasser:	Ppyun, Hyewoo, Kim, Inhye, Cho, Sung Suk, Seo, Kang Jin, Yoon, Keejung, Kwon, Suk-Tae
Format:	Artikel
Sprache:	eng
Schlagworte:	biotechnology Cloning, Molecular commercialization DNA DNA - chemistry DNA - metabolism DNA-binding proteins DNA-directed DNA polymerase DNA-Directed DNA Polymerase - chemistry DNA-Directed DNA Polymerase - genetics DNA-Directed DNA Polymerase - metabolism Escherichia coli - genetics Fidelity Hydrogen-Ion Concentration Kinetics Magnesium Chloride - chemistry mutants Mutation PCR amplification Plasmids - chemistry Plasmids - metabolism polymerase chain reaction Polymerase chain reaction (PCR) Polymerase Chain Reaction - methods Potassium Chloride - chemistry Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism site-directed mutagenesis Thermococcus Thermococcus - enzymology Thermococcus - genetics Thermococcus pacificus-S (Tpa-S) Tpa-S DNA polymerase Tpa-S N213D/K501R DNA polymerase
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