Nitrogen Starvation, Salt and Heat Stress in Coffee (Coffea arabica L.): Identification and Validation of New Genes for qPCR Normalization

Abiotic stresses are among the most important factors that affect food production. One important step to face these environmental challenges is the transcriptional modulation. Quantitative real-time PCR is a rapid, sensitive, and reliable method for the detection of mRNAs and it has become a powerfu...

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Veröffentlicht in:	Molecular biotechnology 2013-03, Vol.53 (3), p.315-325
Hauptverfasser:	de Carvalho, Kenia, Bespalhok Filho, João Carlos, dos Santos, Tiago Benedito, de Souza, Silvia Graciele Hülse, Vieira, Luiz Gonzaga Esteves, Pereira, Luis Filipe Protasio, Domingues, Douglas Silva
Format:	Artikel
Sprache:	eng
Schlagworte:	Abiotic stress Agricultural biotechnology Biochemistry Biological Techniques Biotechnology Cell Biology Chemistry Chemistry and Materials Science Coffea Coffea - genetics Coffea - metabolism Coffea arabica Coffee computer software Food Handling Food production Gene Expression Profiling - methods Gene Expression Regulation, Plant genes Genes, Plant heat stress Heat tolerance hemoglobin Hot Temperature Human Genetics messenger RNA Nitrogen Nitrogen - metabolism plant stress Protein Science quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction Reproducibility of Results RNA, Plant - genetics RNA, Plant - isolation & purification salinity Sequence Analysis, RNA Sodium Chloride Software starvation stress tolerance Stress, Physiological transcription (genetics)
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