Isolation and characterization of bacteriocin‐producing bacteria from the intestinal microbiota of elderly Irish subjects
Aims To isolate and characterize bacteriocins produced by predominant species of lactic acid bacteria from faeces of elderly subjects. Methods and Results Screening over 70 000 colonies, from faecal samples collected from 266 subjects, using the indicator organisms Lactobacillus bulgaricus LMG 6901...
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| Veröffentlicht in: | Journal of applied microbiology 2013-03, Vol.114 (3), p.886-898 |
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| container_title | Journal of applied microbiology |
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| creator | Lakshminarayanan, B. Guinane, C.M. O'Connor, P.M. Coakley, M. Hill, C. Stanton, C. O'Toole, P.W. Ross, R.P. |
| description | Aims
To isolate and characterize bacteriocins produced by predominant species of lactic acid bacteria from faeces of elderly subjects.
Methods and Results
Screening over 70 000 colonies, from faecal samples collected from 266 subjects, using the indicator organisms Lactobacillus bulgaricus LMG 6901 and Listeria innocua DPC 3572, identified 55 antimicrobial‐producing bacteria. Genomic fingerprinting following ApaI digestion revealed 15 distinct strains. The antimicrobial activities associated with 13 of the 15 strains were sensitive to protease treatment. The predominant antimicrobial‐producing species were identified as Lactobacillus salivarius, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus crispatus and Enterococcus spp. A number of previously characterized bacteriocins, including ABP‐118 and salivaricin B (from Lact. salivarius), enterocin B (Enterococcus faecium), lactacin B (Lact. acidophilus), gassericin T and a variant of gassericin A (Lact. gasseri), were identified. Interestingly, two antimicrobial‐producing species, not generally associated with intestinally derived microorganisms were also isolated: Lactococcus lactis producing nisin Z and Streptococcus mutans producing mutacin II.
Conclusion
These data suggest that bacteriocin production by intestinal isolates against our chosen targets under the screening conditions used was not frequent (0·08%).
Significance and Impact of the Study
The results presented are important due to growing evidence indicating bacteriocin production as a potential probiotic trait by virtue of strain dominance and/or pathogen inhibition in the mammalian intestine. |
| doi_str_mv | 10.1111/jam.12085 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1315621182</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1289476279</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4515-b21a84135027f3941bc698b3c6bacf9dc485d61e55dd633513aba565f97a088a3</originalsourceid><addsrcrecordid>eNqFkc1u1DAUhS1ERX9gwQugSAipLNL62rFjL6uKn0Gt2MA6unEcxiMnLnYiNLDhEXhGngRPM6USUoU3vjr6dO6xDyHPgZ5BPucbHM6AUSUekSPgUpRM1uzx7VyVgtbskByntKEUOBXyCTlkHBQIqo_Ij1UKHicXxgLHrjBrjGgmG933RQx90S5CMG78_fPXTQzdnMcvdzoWfQxDMa1t4cbJpsmN6IvBmRhaFybcWVjf2ei3xSq6tC7S3G6smdJTctCjT_bZ_j4hn9---XT5vrz6-G51eXFVmkqAKFsGqCrggrK657qC1kitWm5kTtDrzlRKdBKsEF0nORfAsUUhRa9rpEohPyGni2_O_nXOCZvBJWO9x9GGOTXAQUgGoNj_UaZ0VUtW64y-_AfdhDnmt2eq4qBrLWSdqdcLlb8jpWj75ia6AeO2Adrsymtyec1teZl9sXec28F2f8m7tjLwag9gMuj7iKNx6Z6Tuq4E3y09X7hvztvtwxubDxfXy-o_k2Cx3w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1431979567</pqid></control><display><type>article</type><title>Isolation and characterization of bacteriocin‐producing bacteria from the intestinal microbiota of elderly Irish subjects</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Lakshminarayanan, B. ; Guinane, C.M. ; O'Connor, P.M. ; Coakley, M. ; Hill, C. ; Stanton, C. ; O'Toole, P.W. ; Ross, R.P.</creator><creatorcontrib>Lakshminarayanan, B. ; Guinane, C.M. ; O'Connor, P.M. ; Coakley, M. ; Hill, C. ; Stanton, C. ; O'Toole, P.W. ; Ross, R.P.</creatorcontrib><description>Aims
To isolate and characterize bacteriocins produced by predominant species of lactic acid bacteria from faeces of elderly subjects.
Methods and Results
Screening over 70 000 colonies, from faecal samples collected from 266 subjects, using the indicator organisms Lactobacillus bulgaricus LMG 6901 and Listeria innocua DPC 3572, identified 55 antimicrobial‐producing bacteria. Genomic fingerprinting following ApaI digestion revealed 15 distinct strains. The antimicrobial activities associated with 13 of the 15 strains were sensitive to protease treatment. The predominant antimicrobial‐producing species were identified as Lactobacillus salivarius, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus crispatus and Enterococcus spp. A number of previously characterized bacteriocins, including ABP‐118 and salivaricin B (from Lact. salivarius), enterocin B (Enterococcus faecium), lactacin B (Lact. acidophilus), gassericin T and a variant of gassericin A (Lact. gasseri), were identified. Interestingly, two antimicrobial‐producing species, not generally associated with intestinally derived microorganisms were also isolated: Lactococcus lactis producing nisin Z and Streptococcus mutans producing mutacin II.
Conclusion
These data suggest that bacteriocin production by intestinal isolates against our chosen targets under the screening conditions used was not frequent (0·08%).
Significance and Impact of the Study
The results presented are important due to growing evidence indicating bacteriocin production as a potential probiotic trait by virtue of strain dominance and/or pathogen inhibition in the mammalian intestine.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.12085</identifier><identifier>PMID: 23181509</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>16S rDNA gene amplicon sequencing ; Aged ; Bacteria ; Bacterial Proteins - isolation & purification ; bacteriocins ; Bacteriocins - biosynthesis ; Bacteriocins - isolation & purification ; Biological and medical sciences ; DNA Fingerprinting ; DNA, Bacterial - genetics ; elderly ; Enterococcus ; Enterococcus - genetics ; Enterococcus - isolation & purification ; Enterococcus - metabolism ; Enterococcus faecium ; Feces ; Feces - microbiology ; Fundamental and applied biological sciences. Psychology ; Humans ; intestinal microbiota ; Intestines - microbiology ; Ireland ; Lactobacillus - genetics ; Lactobacillus - isolation & purification ; Lactobacillus - metabolism ; Lactobacillus bulgaricus ; Lactobacillus spp ; Lactococcus lactis ; Listeria innocua ; Microbiology ; Microbiota ; Older people ; Pathology ; Probiotics ; RNA, Ribosomal, 16S - genetics ; Streptococcus mutans</subject><ispartof>Journal of applied microbiology, 2013-03, Vol.114 (3), p.886-898</ispartof><rights>2013 The Society for Applied Microbiology</rights><rights>2014 INIST-CNRS</rights><rights>2013 The Society for Applied Microbiology.</rights><rights>Copyright © 2013 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4515-b21a84135027f3941bc698b3c6bacf9dc485d61e55dd633513aba565f97a088a3</citedby><cites>FETCH-LOGICAL-c4515-b21a84135027f3941bc698b3c6bacf9dc485d61e55dd633513aba565f97a088a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.12085$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.12085$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26974537$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23181509$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lakshminarayanan, B.</creatorcontrib><creatorcontrib>Guinane, C.M.</creatorcontrib><creatorcontrib>O'Connor, P.M.</creatorcontrib><creatorcontrib>Coakley, M.</creatorcontrib><creatorcontrib>Hill, C.</creatorcontrib><creatorcontrib>Stanton, C.</creatorcontrib><creatorcontrib>O'Toole, P.W.</creatorcontrib><creatorcontrib>Ross, R.P.</creatorcontrib><title>Isolation and characterization of bacteriocin‐producing bacteria from the intestinal microbiota of elderly Irish subjects</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
To isolate and characterize bacteriocins produced by predominant species of lactic acid bacteria from faeces of elderly subjects.
Methods and Results
Screening over 70 000 colonies, from faecal samples collected from 266 subjects, using the indicator organisms Lactobacillus bulgaricus LMG 6901 and Listeria innocua DPC 3572, identified 55 antimicrobial‐producing bacteria. Genomic fingerprinting following ApaI digestion revealed 15 distinct strains. The antimicrobial activities associated with 13 of the 15 strains were sensitive to protease treatment. The predominant antimicrobial‐producing species were identified as Lactobacillus salivarius, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus crispatus and Enterococcus spp. A number of previously characterized bacteriocins, including ABP‐118 and salivaricin B (from Lact. salivarius), enterocin B (Enterococcus faecium), lactacin B (Lact. acidophilus), gassericin T and a variant of gassericin A (Lact. gasseri), were identified. Interestingly, two antimicrobial‐producing species, not generally associated with intestinally derived microorganisms were also isolated: Lactococcus lactis producing nisin Z and Streptococcus mutans producing mutacin II.
Conclusion
These data suggest that bacteriocin production by intestinal isolates against our chosen targets under the screening conditions used was not frequent (0·08%).
Significance and Impact of the Study
The results presented are important due to growing evidence indicating bacteriocin production as a potential probiotic trait by virtue of strain dominance and/or pathogen inhibition in the mammalian intestine.</description><subject>16S rDNA gene amplicon sequencing</subject><subject>Aged</subject><subject>Bacteria</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>bacteriocins</subject><subject>Bacteriocins - biosynthesis</subject><subject>Bacteriocins - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>DNA Fingerprinting</subject><subject>DNA, Bacterial - genetics</subject><subject>elderly</subject><subject>Enterococcus</subject><subject>Enterococcus - genetics</subject><subject>Enterococcus - isolation & purification</subject><subject>Enterococcus - metabolism</subject><subject>Enterococcus faecium</subject><subject>Feces</subject><subject>Feces - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>intestinal microbiota</subject><subject>Intestines - microbiology</subject><subject>Ireland</subject><subject>Lactobacillus - genetics</subject><subject>Lactobacillus - isolation & purification</subject><subject>Lactobacillus - metabolism</subject><subject>Lactobacillus bulgaricus</subject><subject>Lactobacillus spp</subject><subject>Lactococcus lactis</subject><subject>Listeria innocua</subject><subject>Microbiology</subject><subject>Microbiota</subject><subject>Older people</subject><subject>Pathology</subject><subject>Probiotics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Streptococcus mutans</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS1ERX9gwQugSAipLNL62rFjL6uKn0Gt2MA6unEcxiMnLnYiNLDhEXhGngRPM6USUoU3vjr6dO6xDyHPgZ5BPucbHM6AUSUekSPgUpRM1uzx7VyVgtbskByntKEUOBXyCTlkHBQIqo_Ij1UKHicXxgLHrjBrjGgmG933RQx90S5CMG78_fPXTQzdnMcvdzoWfQxDMa1t4cbJpsmN6IvBmRhaFybcWVjf2ei3xSq6tC7S3G6smdJTctCjT_bZ_j4hn9---XT5vrz6-G51eXFVmkqAKFsGqCrggrK657qC1kitWm5kTtDrzlRKdBKsEF0nORfAsUUhRa9rpEohPyGni2_O_nXOCZvBJWO9x9GGOTXAQUgGoNj_UaZ0VUtW64y-_AfdhDnmt2eq4qBrLWSdqdcLlb8jpWj75ia6AeO2Adrsymtyec1teZl9sXec28F2f8m7tjLwag9gMuj7iKNx6Z6Tuq4E3y09X7hvztvtwxubDxfXy-o_k2Cx3w</recordid><startdate>201303</startdate><enddate>201303</enddate><creator>Lakshminarayanan, B.</creator><creator>Guinane, C.M.</creator><creator>O'Connor, P.M.</creator><creator>Coakley, M.</creator><creator>Hill, C.</creator><creator>Stanton, C.</creator><creator>O'Toole, P.W.</creator><creator>Ross, R.P.</creator><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7QH</scope><scope>7UA</scope></search><sort><creationdate>201303</creationdate><title>Isolation and characterization of bacteriocin‐producing bacteria from the intestinal microbiota of elderly Irish subjects</title><author>Lakshminarayanan, B. ; Guinane, C.M. ; O'Connor, P.M. ; Coakley, M. ; Hill, C. ; Stanton, C. ; O'Toole, P.W. ; Ross, R.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4515-b21a84135027f3941bc698b3c6bacf9dc485d61e55dd633513aba565f97a088a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>16S rDNA gene amplicon sequencing</topic><topic>Aged</topic><topic>Bacteria</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>bacteriocins</topic><topic>Bacteriocins - biosynthesis</topic><topic>Bacteriocins - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - genetics</topic><topic>elderly</topic><topic>Enterococcus</topic><topic>Enterococcus - genetics</topic><topic>Enterococcus - isolation & purification</topic><topic>Enterococcus - metabolism</topic><topic>Enterococcus faecium</topic><topic>Feces</topic><topic>Feces - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>intestinal microbiota</topic><topic>Intestines - microbiology</topic><topic>Ireland</topic><topic>Lactobacillus - genetics</topic><topic>Lactobacillus - isolation & purification</topic><topic>Lactobacillus - metabolism</topic><topic>Lactobacillus bulgaricus</topic><topic>Lactobacillus spp</topic><topic>Lactococcus lactis</topic><topic>Listeria innocua</topic><topic>Microbiology</topic><topic>Microbiota</topic><topic>Older people</topic><topic>Pathology</topic><topic>Probiotics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Streptococcus mutans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lakshminarayanan, B.</creatorcontrib><creatorcontrib>Guinane, C.M.</creatorcontrib><creatorcontrib>O'Connor, P.M.</creatorcontrib><creatorcontrib>Coakley, M.</creatorcontrib><creatorcontrib>Hill, C.</creatorcontrib><creatorcontrib>Stanton, C.</creatorcontrib><creatorcontrib>O'Toole, P.W.</creatorcontrib><creatorcontrib>Ross, R.P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lakshminarayanan, B.</au><au>Guinane, C.M.</au><au>O'Connor, P.M.</au><au>Coakley, M.</au><au>Hill, C.</au><au>Stanton, C.</au><au>O'Toole, P.W.</au><au>Ross, R.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of bacteriocin‐producing bacteria from the intestinal microbiota of elderly Irish subjects</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2013-03</date><risdate>2013</risdate><volume>114</volume><issue>3</issue><spage>886</spage><epage>898</epage><pages>886-898</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>Aims
To isolate and characterize bacteriocins produced by predominant species of lactic acid bacteria from faeces of elderly subjects.
Methods and Results
Screening over 70 000 colonies, from faecal samples collected from 266 subjects, using the indicator organisms Lactobacillus bulgaricus LMG 6901 and Listeria innocua DPC 3572, identified 55 antimicrobial‐producing bacteria. Genomic fingerprinting following ApaI digestion revealed 15 distinct strains. The antimicrobial activities associated with 13 of the 15 strains were sensitive to protease treatment. The predominant antimicrobial‐producing species were identified as Lactobacillus salivarius, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus crispatus and Enterococcus spp. A number of previously characterized bacteriocins, including ABP‐118 and salivaricin B (from Lact. salivarius), enterocin B (Enterococcus faecium), lactacin B (Lact. acidophilus), gassericin T and a variant of gassericin A (Lact. gasseri), were identified. Interestingly, two antimicrobial‐producing species, not generally associated with intestinally derived microorganisms were also isolated: Lactococcus lactis producing nisin Z and Streptococcus mutans producing mutacin II.
Conclusion
These data suggest that bacteriocin production by intestinal isolates against our chosen targets under the screening conditions used was not frequent (0·08%).
Significance and Impact of the Study
The results presented are important due to growing evidence indicating bacteriocin production as a potential probiotic trait by virtue of strain dominance and/or pathogen inhibition in the mammalian intestine.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>23181509</pmid><doi>10.1111/jam.12085</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of applied microbiology, 2013-03, Vol.114 (3), p.886-898 |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library All Journals |
| subjects | 16S rDNA gene amplicon sequencing Aged Bacteria Bacterial Proteins - isolation & purification bacteriocins Bacteriocins - biosynthesis Bacteriocins - isolation & purification Biological and medical sciences DNA Fingerprinting DNA, Bacterial - genetics elderly Enterococcus Enterococcus - genetics Enterococcus - isolation & purification Enterococcus - metabolism Enterococcus faecium Feces Feces - microbiology Fundamental and applied biological sciences. Psychology Humans intestinal microbiota Intestines - microbiology Ireland Lactobacillus - genetics Lactobacillus - isolation & purification Lactobacillus - metabolism Lactobacillus bulgaricus Lactobacillus spp Lactococcus lactis Listeria innocua Microbiology Microbiota Older people Pathology Probiotics RNA, Ribosomal, 16S - genetics Streptococcus mutans |
| title | Isolation and characterization of bacteriocin‐producing bacteria from the intestinal microbiota of elderly Irish subjects |
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