Analysis of the 3′-variable region of the cagA gene from Helicobacter pylori strains infecting patients at New York City hospitals
Helicobacter pylori infects the gastric mucosa in humans and is a causative agent for peptic ulcer disease (PUD) and gastric cancer (GC). CagA is produced by H. pylori and is associated with more severe outcomes. cagA genes vary at the 3′-region with respect to phosphorylation motifs (EPIYA-A, -B, -...
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| description | Helicobacter pylori infects the gastric mucosa in humans and is a causative agent for peptic ulcer disease (PUD) and gastric cancer (GC). CagA is produced by H. pylori and is associated with more severe outcomes. cagA genes vary at the 3′-region with respect to phosphorylation motifs (EPIYA-A, -B, -C, or -D) and CagA multimerization motifs (CM). This variability may be associated with the clinical outcomes. We examined the variable region of cagA genes expressed in H. pylori-infected patients treated at three NYC Hospitals. DNA was isolated from gastric biopsies of patients undergoing upper endoscopy. Most H. pylori-infected patients were Black or Hispanic. The cagA 3′-region of CagA-positive samples was amplified by PCR, purified and sequenced. The patterns of EPIYA and CM motifs were examined and related to clinical outcomes. We obtained 42 CagA sequences from our sample collection. The EPIYA phosphorylation motif pattern was ABC in 81.0% of our samples. Western (W) and Eastern (E) CM motifs have also been defined. CagA proteins lacking an Eastern CM motif and possessing one or two Western CM motifs were observed more frequently in patients with PUD and GC when compared with non-ulcer gastritis (50.0% vs 11.8%, respectively), suggesting that these CM motif patterns are more virulent than those containing at least one Eastern CM motif. We conclude that In H. pylori-infected patients treated at NYC Hospitals, CM motif patterns in the CagA 30-variable region may be more significant than EPIYA motif patterns with respect to clinical outcomes.
► cagA genes expressed in Helicobacter pylori-infected patients at NYC Hospitals were examined. ► The EPIYA phosphorylation motif pattern was ABC in 81.0% of our samples. ► Two CagA multimerization (CM) motifs were present in 91% of the samples. ► CagA proteins lacking an Eastern CM motif were associated with more severe disorders. ► CM motif patterns may be clinically relevant in our population. |
| doi_str_mv | 10.1016/j.micpath.2012.10.003 |
| format | Article |
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► cagA genes expressed in Helicobacter pylori-infected patients at NYC Hospitals were examined. ► The EPIYA phosphorylation motif pattern was ABC in 81.0% of our samples. ► Two CagA multimerization (CM) motifs were present in 91% of the samples. ► CagA proteins lacking an Eastern CM motif were associated with more severe disorders. ► CM motif patterns may be clinically relevant in our population.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2012.10.003</identifier><identifier>PMID: 23117095</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Motifs ; Amino Acid Sequence ; Antigens, Bacterial - genetics ; Bacterial Proteins - genetics ; Biopsy ; CagA ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Gastric cancer ; Gastric Mucosa - microbiology ; H. pylori ; Helicobacter Infections - microbiology ; Helicobacter Infections - pathology ; Helicobacter pylori ; Helicobacter pylori - genetics ; Helicobacter pylori - isolation & purification ; Hospitals ; Humans ; Molecular Sequence Data ; New York City ; Peptic Ulcer - microbiology ; Peptic Ulcer - pathology ; Peptic ulcer disease ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Sequence Alignment ; Sequence Analysis, DNA ; Stomach Neoplasms - microbiology ; Stomach Neoplasms - pathology ; Virulence Factors - genetics</subject><ispartof>Microbial pathogenesis, 2013-03, Vol.56, p.29-34</ispartof><rights>2012</rights><rights>Copyright © 2012. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-3a48525e4299828bf5d72e85b8521242b9bd067f5b94f6798fde3ebb49c40d43</citedby><cites>FETCH-LOGICAL-c464t-3a48525e4299828bf5d72e85b8521242b9bd067f5b94f6798fde3ebb49c40d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.micpath.2012.10.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23117095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ogorodnik, Evgeny</creatorcontrib><creatorcontrib>Raffaniello, Robert D.</creatorcontrib><title>Analysis of the 3′-variable region of the cagA gene from Helicobacter pylori strains infecting patients at New York City hospitals</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Helicobacter pylori infects the gastric mucosa in humans and is a causative agent for peptic ulcer disease (PUD) and gastric cancer (GC). CagA is produced by H. pylori and is associated with more severe outcomes. cagA genes vary at the 3′-region with respect to phosphorylation motifs (EPIYA-A, -B, -C, or -D) and CagA multimerization motifs (CM). This variability may be associated with the clinical outcomes. We examined the variable region of cagA genes expressed in H. pylori-infected patients treated at three NYC Hospitals. DNA was isolated from gastric biopsies of patients undergoing upper endoscopy. Most H. pylori-infected patients were Black or Hispanic. The cagA 3′-region of CagA-positive samples was amplified by PCR, purified and sequenced. The patterns of EPIYA and CM motifs were examined and related to clinical outcomes. We obtained 42 CagA sequences from our sample collection. The EPIYA phosphorylation motif pattern was ABC in 81.0% of our samples. Western (W) and Eastern (E) CM motifs have also been defined. CagA proteins lacking an Eastern CM motif and possessing one or two Western CM motifs were observed more frequently in patients with PUD and GC when compared with non-ulcer gastritis (50.0% vs 11.8%, respectively), suggesting that these CM motif patterns are more virulent than those containing at least one Eastern CM motif. We conclude that In H. pylori-infected patients treated at NYC Hospitals, CM motif patterns in the CagA 30-variable region may be more significant than EPIYA motif patterns with respect to clinical outcomes.
► cagA genes expressed in Helicobacter pylori-infected patients at NYC Hospitals were examined. ► The EPIYA phosphorylation motif pattern was ABC in 81.0% of our samples. ► Two CagA multimerization (CM) motifs were present in 91% of the samples. ► CagA proteins lacking an Eastern CM motif were associated with more severe disorders. ► CM motif patterns may be clinically relevant in our population.</description><subject>Amino Acid Motifs</subject><subject>Amino Acid Sequence</subject><subject>Antigens, Bacterial - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Biopsy</subject><subject>CagA</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Gastric cancer</subject><subject>Gastric Mucosa - microbiology</subject><subject>H. pylori</subject><subject>Helicobacter Infections - microbiology</subject><subject>Helicobacter Infections - pathology</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - genetics</subject><subject>Helicobacter pylori - isolation & purification</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>New York City</subject><subject>Peptic Ulcer - microbiology</subject><subject>Peptic Ulcer - pathology</subject><subject>Peptic ulcer disease</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Genetic</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Stomach Neoplasms - microbiology</subject><subject>Stomach Neoplasms - pathology</subject><subject>Virulence Factors - genetics</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb2OEzEUhS0EYsPCI4Bc0kzw30zsCkURsEgraLahsmzPdeIwMx5sZ1E6Cp6IR-JJcJQs7VZXOve7PzoHodeULCmh3bv9cgxuNmW3ZISyqi0J4U_QghLVNZQR-RQtiJSsEYSSK_Qi5z0hRAmunqMrxildEdUu0O_1ZIZjDhlHj8sOMP_7609zb1IwdgCcYBvi9NBzZrvGW5gA-xRHfANDcNEaVyDh-TjEFHAuyYQp4zB5cCVMW1x_DDCVjE3BX-An_hbTd7wJ5Yh3Mc-hmCG_RM98LfDqUq_R3ccPd5ub5vbrp8-b9W3jRCdKw42QLWtBMKUkk9a3_YqBbG1VKRPMKtuTbuVbq4TvVkr6HjhYK5QTpBf8Gr09r51T_HGAXPQYsoNhMBPEQ9aU07ajsuXkcZRJVZ9R8oS2Z9SlmHMCr-cURpOOmhJ9ikrv9SUqfYrqJNeo6tyby4mDHaH_P_WQTQXenwGoltwHSDq76qSDPqTqre5jeOTEP-BUqPk</recordid><startdate>201303</startdate><enddate>201303</enddate><creator>Ogorodnik, Evgeny</creator><creator>Raffaniello, Robert D.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201303</creationdate><title>Analysis of the 3′-variable region of the cagA gene from Helicobacter pylori strains infecting patients at New York City hospitals</title><author>Ogorodnik, Evgeny ; Raffaniello, Robert D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-3a48525e4299828bf5d72e85b8521242b9bd067f5b94f6798fde3ebb49c40d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amino Acid Motifs</topic><topic>Amino Acid Sequence</topic><topic>Antigens, Bacterial - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Biopsy</topic><topic>CagA</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Gastric cancer</topic><topic>Gastric Mucosa - microbiology</topic><topic>H. pylori</topic><topic>Helicobacter Infections - microbiology</topic><topic>Helicobacter Infections - pathology</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - genetics</topic><topic>Helicobacter pylori - isolation & purification</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>New York City</topic><topic>Peptic Ulcer - microbiology</topic><topic>Peptic Ulcer - pathology</topic><topic>Peptic ulcer disease</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Genetic</topic><topic>Sequence Alignment</topic><topic>Sequence Analysis, DNA</topic><topic>Stomach Neoplasms - microbiology</topic><topic>Stomach Neoplasms - pathology</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ogorodnik, Evgeny</creatorcontrib><creatorcontrib>Raffaniello, Robert D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ogorodnik, Evgeny</au><au>Raffaniello, Robert D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of the 3′-variable region of the cagA gene from Helicobacter pylori strains infecting patients at New York City hospitals</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2013-03</date><risdate>2013</risdate><volume>56</volume><spage>29</spage><epage>34</epage><pages>29-34</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>Helicobacter pylori infects the gastric mucosa in humans and is a causative agent for peptic ulcer disease (PUD) and gastric cancer (GC). CagA is produced by H. pylori and is associated with more severe outcomes. cagA genes vary at the 3′-region with respect to phosphorylation motifs (EPIYA-A, -B, -C, or -D) and CagA multimerization motifs (CM). This variability may be associated with the clinical outcomes. We examined the variable region of cagA genes expressed in H. pylori-infected patients treated at three NYC Hospitals. DNA was isolated from gastric biopsies of patients undergoing upper endoscopy. Most H. pylori-infected patients were Black or Hispanic. The cagA 3′-region of CagA-positive samples was amplified by PCR, purified and sequenced. The patterns of EPIYA and CM motifs were examined and related to clinical outcomes. We obtained 42 CagA sequences from our sample collection. The EPIYA phosphorylation motif pattern was ABC in 81.0% of our samples. Western (W) and Eastern (E) CM motifs have also been defined. CagA proteins lacking an Eastern CM motif and possessing one or two Western CM motifs were observed more frequently in patients with PUD and GC when compared with non-ulcer gastritis (50.0% vs 11.8%, respectively), suggesting that these CM motif patterns are more virulent than those containing at least one Eastern CM motif. We conclude that In H. pylori-infected patients treated at NYC Hospitals, CM motif patterns in the CagA 30-variable region may be more significant than EPIYA motif patterns with respect to clinical outcomes.
► cagA genes expressed in Helicobacter pylori-infected patients at NYC Hospitals were examined. ► The EPIYA phosphorylation motif pattern was ABC in 81.0% of our samples. ► Two CagA multimerization (CM) motifs were present in 91% of the samples. ► CagA proteins lacking an Eastern CM motif were associated with more severe disorders. ► CM motif patterns may be clinically relevant in our population.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23117095</pmid><doi>10.1016/j.micpath.2012.10.003</doi><tpages>6</tpages></addata></record> |
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| subjects | Amino Acid Motifs Amino Acid Sequence Antigens, Bacterial - genetics Bacterial Proteins - genetics Biopsy CagA DNA, Bacterial - chemistry DNA, Bacterial - genetics Gastric cancer Gastric Mucosa - microbiology H. pylori Helicobacter Infections - microbiology Helicobacter Infections - pathology Helicobacter pylori Helicobacter pylori - genetics Helicobacter pylori - isolation & purification Hospitals Humans Molecular Sequence Data New York City Peptic Ulcer - microbiology Peptic Ulcer - pathology Peptic ulcer disease Polymerase Chain Reaction Polymorphism, Genetic Sequence Alignment Sequence Analysis, DNA Stomach Neoplasms - microbiology Stomach Neoplasms - pathology Virulence Factors - genetics |
| title | Analysis of the 3′-variable region of the cagA gene from Helicobacter pylori strains infecting patients at New York City hospitals |
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