Polyvinyl alcohol and polyethylene glycol form polymer bodies in the periplasm of Sphingomonads that are able to assimilate them
Scanning electron microscopy (SEM) shows remarkable morphological surface changes in Sphingopyxis sp. 113P3 cells grown in polyvinyl alcohol (PVA) but not in Luria–Bertani medium (LB) (Hu et al. in Arch Microbiol 188: 235–241, 2007 ). However, transmission electron microscopy showed no surface chang...
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| creator | Kawai, Fusako Kitajima, Sakihito Oda, Kenji Higasa, Takahiko Charoenpanich, Jittima Hu, Xiaoping Mamoto, Rie |
| description | Scanning electron microscopy (SEM) shows remarkable morphological surface changes in
Sphingopyxis
sp. 113P3 cells grown in polyvinyl alcohol (PVA) but not in Luria–Bertani medium (LB) (Hu et al. in Arch Microbiol 188: 235–241,
2007
). However, transmission electron microscopy showed no surface changes in PVA-grown cells and revealed the presence of polymer bodies in the periplasm of PVA-grown cells, which were not observed in LB-grown cells. The presence of polymer bodies was supported by low-vacuum SEM observation of PVA- and LB-grown cells of strain 113P3, and the presence of similar polymer bodies was also found when
Sphingopyxis macrogoltabida
103 and
S. terrae
were grown in polyethylene glycol (PEG). The extraction of PVA and PEG from the periplasmic fraction of cells using a modified Anraku and Heppel method and their analysis by MALDI–TOF mass spectrometry strongly suggested that the polymer bodies are composed of PVA and PEG, respectively, in
Sphingopyxis
sp. 113P3 (PVA degrader) and
Sphingopyxis macrogoltabida
103 or
S
.
terrae
(PEG degraders). PEG-grown
S. macrogoltabida
103 and
S. terrae
showed higher transport of
14
C-PEG 4000 than LB-grown cells. Recombinant PegB (TonB-dependent receptor-like protein consisting of a barrel structure) interacted with PEG 200, 4000 and 20000, suggesting that the barrel protein in the outer membrane contributes to the transport of PEG into the periplasm. |
| doi_str_mv | 10.1007/s00203-012-0859-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1315618069</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1283274064</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-f19c52a343977c6334952fde3ceab5a6e35dc4c043363c9da1d061939d0ca9483</originalsourceid><addsrcrecordid>eNqFkU2LFDEQhoMo7uzoD_AiAS9eWpNU-iNHWfyCBRd2BW9NJqmeyZJ02qRnoW_-dNPOKiLI5lKk6qm3qngJecHZG85Y-zYzJhhUjIuKdbWq-COy4RLKrxXfHpMNAyaqTgGckfOcb1kBu657Ss4EiAbK25AfV9Evd25cPNXexEMscbR0KlmcD4vHEeneL6bkh5jCr0LARHfROszUjXQ-IJ0wucnrHGgc6PV0cOM-hjhqm0tZz1QnpHrnkc6R6pxdcF7PuLaGZ-TJoH3G5_dxS75-eH9z8am6_PLx88W7y8pIVs_VwJWphQYJqm1NWV6qWgwWwaDe1bpBqK2RhkmABoyymlvWcAXKMqOV7GBLXp90pxS_HzHPfXDZoPd6xHjMPQdeN7xjjXoYFR2IVrJGFvTVP-htPKaxHLJSoi478HU2P1EmxZwTDv2UXNBp6TnrVyf7k5N9MahfnSzbbMnLe-XjLqD90_HbugKIE5BLadxj-mv0f1V_Ag9UqYE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1282506118</pqid></control><display><type>article</type><title>Polyvinyl alcohol and polyethylene glycol form polymer bodies in the periplasm of Sphingomonads that are able to assimilate them</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Kawai, Fusako ; Kitajima, Sakihito ; Oda, Kenji ; Higasa, Takahiko ; Charoenpanich, Jittima ; Hu, Xiaoping ; Mamoto, Rie</creator><creatorcontrib>Kawai, Fusako ; Kitajima, Sakihito ; Oda, Kenji ; Higasa, Takahiko ; Charoenpanich, Jittima ; Hu, Xiaoping ; Mamoto, Rie</creatorcontrib><description>Scanning electron microscopy (SEM) shows remarkable morphological surface changes in
Sphingopyxis
sp. 113P3 cells grown in polyvinyl alcohol (PVA) but not in Luria–Bertani medium (LB) (Hu et al. in Arch Microbiol 188: 235–241,
2007
). However, transmission electron microscopy showed no surface changes in PVA-grown cells and revealed the presence of polymer bodies in the periplasm of PVA-grown cells, which were not observed in LB-grown cells. The presence of polymer bodies was supported by low-vacuum SEM observation of PVA- and LB-grown cells of strain 113P3, and the presence of similar polymer bodies was also found when
Sphingopyxis macrogoltabida
103 and
S. terrae
were grown in polyethylene glycol (PEG). The extraction of PVA and PEG from the periplasmic fraction of cells using a modified Anraku and Heppel method and their analysis by MALDI–TOF mass spectrometry strongly suggested that the polymer bodies are composed of PVA and PEG, respectively, in
Sphingopyxis
sp. 113P3 (PVA degrader) and
Sphingopyxis macrogoltabida
103 or
S
.
terrae
(PEG degraders). PEG-grown
S. macrogoltabida
103 and
S. terrae
showed higher transport of
14
C-PEG 4000 than LB-grown cells. Recombinant PegB (TonB-dependent receptor-like protein consisting of a barrel structure) interacted with PEG 200, 4000 and 20000, suggesting that the barrel protein in the outer membrane contributes to the transport of PEG into the periplasm.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-012-0859-1</identifier><identifier>PMID: 23263333</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Bacterial Proteins - metabolism ; Biochemistry ; Biodegradation ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Dehydrogenases ; Ecology ; Enzymes ; Life Sciences ; Mass spectrometry ; Membrane Proteins - metabolism ; Metabolism ; Microbial Ecology ; Microbiology ; Microorganisms ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Molecular weight ; Original Paper ; Periplasm - chemistry ; Polyethylene glycol ; Polyethylene Glycols - chemistry ; Polymers ; Polymers - chemistry ; Polyvinyl alcohol ; Polyvinyl Alcohol - chemistry ; Proteins ; Scanning electron microscopy ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Sphingomonadaceae - chemistry ; Sphingomonadaceae - ultrastructure ; Sphingopyxis macrogoltabida</subject><ispartof>Archives of microbiology, 2013-02, Vol.195 (2), p.131-140</ispartof><rights>Springer-Verlag Berlin Heidelberg 2012</rights><rights>Springer-Verlag Berlin Heidelberg 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-f19c52a343977c6334952fde3ceab5a6e35dc4c043363c9da1d061939d0ca9483</citedby><cites>FETCH-LOGICAL-c405t-f19c52a343977c6334952fde3ceab5a6e35dc4c043363c9da1d061939d0ca9483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-012-0859-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-012-0859-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23263333$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawai, Fusako</creatorcontrib><creatorcontrib>Kitajima, Sakihito</creatorcontrib><creatorcontrib>Oda, Kenji</creatorcontrib><creatorcontrib>Higasa, Takahiko</creatorcontrib><creatorcontrib>Charoenpanich, Jittima</creatorcontrib><creatorcontrib>Hu, Xiaoping</creatorcontrib><creatorcontrib>Mamoto, Rie</creatorcontrib><title>Polyvinyl alcohol and polyethylene glycol form polymer bodies in the periplasm of Sphingomonads that are able to assimilate them</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>Scanning electron microscopy (SEM) shows remarkable morphological surface changes in
Sphingopyxis
sp. 113P3 cells grown in polyvinyl alcohol (PVA) but not in Luria–Bertani medium (LB) (Hu et al. in Arch Microbiol 188: 235–241,
2007
). However, transmission electron microscopy showed no surface changes in PVA-grown cells and revealed the presence of polymer bodies in the periplasm of PVA-grown cells, which were not observed in LB-grown cells. The presence of polymer bodies was supported by low-vacuum SEM observation of PVA- and LB-grown cells of strain 113P3, and the presence of similar polymer bodies was also found when
Sphingopyxis macrogoltabida
103 and
S. terrae
were grown in polyethylene glycol (PEG). The extraction of PVA and PEG from the periplasmic fraction of cells using a modified Anraku and Heppel method and their analysis by MALDI–TOF mass spectrometry strongly suggested that the polymer bodies are composed of PVA and PEG, respectively, in
Sphingopyxis
sp. 113P3 (PVA degrader) and
Sphingopyxis macrogoltabida
103 or
S
.
terrae
(PEG degraders). PEG-grown
S. macrogoltabida
103 and
S. terrae
showed higher transport of
14
C-PEG 4000 than LB-grown cells. Recombinant PegB (TonB-dependent receptor-like protein consisting of a barrel structure) interacted with PEG 200, 4000 and 20000, suggesting that the barrel protein in the outer membrane contributes to the transport of PEG into the periplasm.</description><subject>Bacterial Proteins - metabolism</subject><subject>Biochemistry</subject><subject>Biodegradation</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Dehydrogenases</subject><subject>Ecology</subject><subject>Enzymes</subject><subject>Life Sciences</subject><subject>Mass spectrometry</subject><subject>Membrane Proteins - metabolism</subject><subject>Metabolism</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Electron, Transmission</subject><subject>Molecular weight</subject><subject>Original Paper</subject><subject>Periplasm - chemistry</subject><subject>Polyethylene glycol</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Polymers</subject><subject>Polymers - chemistry</subject><subject>Polyvinyl alcohol</subject><subject>Polyvinyl Alcohol - chemistry</subject><subject>Proteins</subject><subject>Scanning electron microscopy</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Sphingomonadaceae - chemistry</subject><subject>Sphingomonadaceae - ultrastructure</subject><subject>Sphingopyxis macrogoltabida</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU2LFDEQhoMo7uzoD_AiAS9eWpNU-iNHWfyCBRd2BW9NJqmeyZJ02qRnoW_-dNPOKiLI5lKk6qm3qngJecHZG85Y-zYzJhhUjIuKdbWq-COy4RLKrxXfHpMNAyaqTgGckfOcb1kBu657Ss4EiAbK25AfV9Evd25cPNXexEMscbR0KlmcD4vHEeneL6bkh5jCr0LARHfROszUjXQ-IJ0wucnrHGgc6PV0cOM-hjhqm0tZz1QnpHrnkc6R6pxdcF7PuLaGZ-TJoH3G5_dxS75-eH9z8am6_PLx88W7y8pIVs_VwJWphQYJqm1NWV6qWgwWwaDe1bpBqK2RhkmABoyymlvWcAXKMqOV7GBLXp90pxS_HzHPfXDZoPd6xHjMPQdeN7xjjXoYFR2IVrJGFvTVP-htPKaxHLJSoi478HU2P1EmxZwTDv2UXNBp6TnrVyf7k5N9MahfnSzbbMnLe-XjLqD90_HbugKIE5BLadxj-mv0f1V_Ag9UqYE</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Kawai, Fusako</creator><creator>Kitajima, Sakihito</creator><creator>Oda, Kenji</creator><creator>Higasa, Takahiko</creator><creator>Charoenpanich, Jittima</creator><creator>Hu, Xiaoping</creator><creator>Mamoto, Rie</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>7QH</scope><scope>7UA</scope></search><sort><creationdate>20130201</creationdate><title>Polyvinyl alcohol and polyethylene glycol form polymer bodies in the periplasm of Sphingomonads that are able to assimilate them</title><author>Kawai, Fusako ; Kitajima, Sakihito ; Oda, Kenji ; Higasa, Takahiko ; Charoenpanich, Jittima ; Hu, Xiaoping ; Mamoto, Rie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-f19c52a343977c6334952fde3ceab5a6e35dc4c043363c9da1d061939d0ca9483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Bacterial Proteins - metabolism</topic><topic>Biochemistry</topic><topic>Biodegradation</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Dehydrogenases</topic><topic>Ecology</topic><topic>Enzymes</topic><topic>Life Sciences</topic><topic>Mass spectrometry</topic><topic>Membrane Proteins - metabolism</topic><topic>Metabolism</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microscopy, Electron, Transmission</topic><topic>Molecular weight</topic><topic>Original Paper</topic><topic>Periplasm - chemistry</topic><topic>Polyethylene glycol</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Polymers</topic><topic>Polymers - chemistry</topic><topic>Polyvinyl alcohol</topic><topic>Polyvinyl Alcohol - chemistry</topic><topic>Proteins</topic><topic>Scanning electron microscopy</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Sphingomonadaceae - chemistry</topic><topic>Sphingomonadaceae - ultrastructure</topic><topic>Sphingopyxis macrogoltabida</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawai, Fusako</creatorcontrib><creatorcontrib>Kitajima, Sakihito</creatorcontrib><creatorcontrib>Oda, Kenji</creatorcontrib><creatorcontrib>Higasa, Takahiko</creatorcontrib><creatorcontrib>Charoenpanich, Jittima</creatorcontrib><creatorcontrib>Hu, Xiaoping</creatorcontrib><creatorcontrib>Mamoto, Rie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawai, Fusako</au><au>Kitajima, Sakihito</au><au>Oda, Kenji</au><au>Higasa, Takahiko</au><au>Charoenpanich, Jittima</au><au>Hu, Xiaoping</au><au>Mamoto, Rie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyvinyl alcohol and polyethylene glycol form polymer bodies in the periplasm of Sphingomonads that are able to assimilate them</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>195</volume><issue>2</issue><spage>131</spage><epage>140</epage><pages>131-140</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>Scanning electron microscopy (SEM) shows remarkable morphological surface changes in
Sphingopyxis
sp. 113P3 cells grown in polyvinyl alcohol (PVA) but not in Luria–Bertani medium (LB) (Hu et al. in Arch Microbiol 188: 235–241,
2007
). However, transmission electron microscopy showed no surface changes in PVA-grown cells and revealed the presence of polymer bodies in the periplasm of PVA-grown cells, which were not observed in LB-grown cells. The presence of polymer bodies was supported by low-vacuum SEM observation of PVA- and LB-grown cells of strain 113P3, and the presence of similar polymer bodies was also found when
Sphingopyxis macrogoltabida
103 and
S. terrae
were grown in polyethylene glycol (PEG). The extraction of PVA and PEG from the periplasmic fraction of cells using a modified Anraku and Heppel method and their analysis by MALDI–TOF mass spectrometry strongly suggested that the polymer bodies are composed of PVA and PEG, respectively, in
Sphingopyxis
sp. 113P3 (PVA degrader) and
Sphingopyxis macrogoltabida
103 or
S
.
terrae
(PEG degraders). PEG-grown
S. macrogoltabida
103 and
S. terrae
showed higher transport of
14
C-PEG 4000 than LB-grown cells. Recombinant PegB (TonB-dependent receptor-like protein consisting of a barrel structure) interacted with PEG 200, 4000 and 20000, suggesting that the barrel protein in the outer membrane contributes to the transport of PEG into the periplasm.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>23263333</pmid><doi>10.1007/s00203-012-0859-1</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0302-8933 |
| ispartof | Archives of microbiology, 2013-02, Vol.195 (2), p.131-140 |
| issn | 0302-8933 1432-072X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1315618069 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Bacterial Proteins - metabolism Biochemistry Biodegradation Biomedical and Life Sciences Biotechnology Cell Biology Dehydrogenases Ecology Enzymes Life Sciences Mass spectrometry Membrane Proteins - metabolism Metabolism Microbial Ecology Microbiology Microorganisms Microscopy, Electron, Scanning Microscopy, Electron, Transmission Molecular weight Original Paper Periplasm - chemistry Polyethylene glycol Polyethylene Glycols - chemistry Polymers Polymers - chemistry Polyvinyl alcohol Polyvinyl Alcohol - chemistry Proteins Scanning electron microscopy Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sphingomonadaceae - chemistry Sphingomonadaceae - ultrastructure Sphingopyxis macrogoltabida |
| title | Polyvinyl alcohol and polyethylene glycol form polymer bodies in the periplasm of Sphingomonads that are able to assimilate them |
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