Rapid screening for Chlamydia trachomatis infection by detecting [alpha]-mannosidase activity in urogenital tract specimens
Chlamydia trachomatis may cause multiple different urogenital tract disorders, but current non-culture assays for rapid screening of C. trachomatis typically use immunochromatography-based methods. We established another new rapid non-culture method for detection of C. trachomatis based on the measu...
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| Veröffentlicht in: | BMC infectious diseases 2013-01, Vol.13 (1), p.36-36 |
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| creator | Wang, Ze-yu Fu, Guang-yu Wang, Shan-mei Qin, Dong-chun Wang, Zhong-quan Cui, Jing |
| description | Chlamydia trachomatis may cause multiple different urogenital tract disorders, but current non-culture assays for rapid screening of C. trachomatis typically use immunochromatography-based methods. We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of [alpha]-mannosidase enzymatic activity in urogenital tract specimens. To evaluate the performance of this method, [alpha]-mannosidase activities of C. trachomatis serotype D strain ã and 29 standard strains related to clinical urogenital pathogens were investigated. Furthermore, 553 urogenital tract specimens were used for clinical assays via cell culture method and ligase chain reaction method (LCR), adopting an expanded gold standard. Only C. trachomatis was positive for [alpha]-mannosidase activity among different types of microbes tested in the research. When prostate fluid specimens, which have some interfering activity, were excluded, the sensitivity and specificity of the enzymatic method were 91.8% (78/85) and 98.3% (409/416), respectively. There were no significant differences (P > 0.05). These results showed that [alpha]-mannosidase activity could be utilised as a screening marker of C. trachomatis infection. |
| doi_str_mv | 10.1186/1471-2334-13-36 |
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We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of [alpha]-mannosidase enzymatic activity in urogenital tract specimens. To evaluate the performance of this method, [alpha]-mannosidase activities of C. trachomatis serotype D strain ã and 29 standard strains related to clinical urogenital pathogens were investigated. Furthermore, 553 urogenital tract specimens were used for clinical assays via cell culture method and ligase chain reaction method (LCR), adopting an expanded gold standard. Only C. trachomatis was positive for [alpha]-mannosidase activity among different types of microbes tested in the research. When prostate fluid specimens, which have some interfering activity, were excluded, the sensitivity and specificity of the enzymatic method were 91.8% (78/85) and 98.3% (409/416), respectively. There were no significant differences (P > 0.05). These results showed that [alpha]-mannosidase activity could be utilised as a screening marker of C. trachomatis infection.</description><identifier>ISSN: 1471-2334</identifier><identifier>EISSN: 1471-2334</identifier><identifier>DOI: 10.1186/1471-2334-13-36</identifier><language>eng</language><publisher>London: BioMed Central Ltd</publisher><subject>Chlamydia ; Chlamydia infections ; Chlamydia trachomatis ; Health aspects ; Infections ; Ligases ; Medical tests ; Methods ; Microbiology ; Sexually transmitted diseases ; STD ; Studies ; Women</subject><ispartof>BMC infectious diseases, 2013-01, Vol.13 (1), p.36-36</ispartof><rights>COPYRIGHT 2013 BioMed Central Ltd.</rights><rights>2013 Wang et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Wang, Ze-yu</creatorcontrib><creatorcontrib>Fu, Guang-yu</creatorcontrib><creatorcontrib>Wang, Shan-mei</creatorcontrib><creatorcontrib>Qin, Dong-chun</creatorcontrib><creatorcontrib>Wang, Zhong-quan</creatorcontrib><creatorcontrib>Cui, Jing</creatorcontrib><title>Rapid screening for Chlamydia trachomatis infection by detecting [alpha]-mannosidase activity in urogenital tract specimens</title><title>BMC infectious diseases</title><description>Chlamydia trachomatis may cause multiple different urogenital tract disorders, but current non-culture assays for rapid screening of C. trachomatis typically use immunochromatography-based methods. We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of [alpha]-mannosidase enzymatic activity in urogenital tract specimens. To evaluate the performance of this method, [alpha]-mannosidase activities of C. trachomatis serotype D strain ã and 29 standard strains related to clinical urogenital pathogens were investigated. Furthermore, 553 urogenital tract specimens were used for clinical assays via cell culture method and ligase chain reaction method (LCR), adopting an expanded gold standard. Only C. trachomatis was positive for [alpha]-mannosidase activity among different types of microbes tested in the research. When prostate fluid specimens, which have some interfering activity, were excluded, the sensitivity and specificity of the enzymatic method were 91.8% (78/85) and 98.3% (409/416), respectively. There were no significant differences (P > 0.05). These results showed that [alpha]-mannosidase activity could be utilised as a screening marker of C. trachomatis infection.</description><subject>Chlamydia</subject><subject>Chlamydia infections</subject><subject>Chlamydia trachomatis</subject><subject>Health aspects</subject><subject>Infections</subject><subject>Ligases</subject><subject>Medical tests</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Sexually transmitted diseases</subject><subject>STD</subject><subject>Studies</subject><subject>Women</subject><issn>1471-2334</issn><issn>1471-2334</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqNkM1rFTEUxQdRsLau3Qbc6GJqPiaZZFke1hYKhda6ERnuy9zMS5lJnpOM-PCfb56KtuKi3EXuPfd3Dkmq6hWjx4xp9Y41Lau5EE3NRC3Uk-rgj_L0Xv-8epHSLaWs1dwcVD-uYOt7kuyMGHwYiIszWW1GmHa9B5JnsJs4QfaJ-ODQZh8DWe9Ij3k_FMNnGLcb-FJPEEJMvoeEBMrqm8-74iHLHIcSnWH8mZZJ2qL1E4Z0VD1zMCZ8-fs8rG5O339cndUXlx_OVycX9cCMkrU22jChtEZFnXW8WQtGmx4Et70DbYzggsrGSbWW67bsqEFwmklrRKsoF4fVm1-52zl-XTDlbvLJ4jhCwLikjgkmFVPGyIK-_ge9jcscyu06xrXkLeO8_UsNMGJX_iXuX7YP7U6kaBRtVasLdfwfqlSPk7cxoPNFf2B4-8BQmIzf8wBLSt359dXj2ctP99k7mEylEA</recordid><startdate>20130124</startdate><enddate>20130124</enddate><creator>Wang, Ze-yu</creator><creator>Fu, Guang-yu</creator><creator>Wang, Shan-mei</creator><creator>Qin, Dong-chun</creator><creator>Wang, Zhong-quan</creator><creator>Cui, Jing</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T2</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20130124</creationdate><title>Rapid screening for Chlamydia trachomatis infection by detecting [alpha]-mannosidase activity in urogenital tract specimens</title><author>Wang, Ze-yu ; Fu, Guang-yu ; Wang, Shan-mei ; Qin, Dong-chun ; Wang, Zhong-quan ; Cui, Jing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g1965-898913688e60fcf24b3104da32cdfa899323054f56b5b731009eaf815c9376023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Chlamydia</topic><topic>Chlamydia infections</topic><topic>Chlamydia trachomatis</topic><topic>Health aspects</topic><topic>Infections</topic><topic>Ligases</topic><topic>Medical tests</topic><topic>Methods</topic><topic>Microbiology</topic><topic>Sexually transmitted diseases</topic><topic>STD</topic><topic>Studies</topic><topic>Women</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Ze-yu</creatorcontrib><creatorcontrib>Fu, Guang-yu</creatorcontrib><creatorcontrib>Wang, Shan-mei</creatorcontrib><creatorcontrib>Qin, Dong-chun</creatorcontrib><creatorcontrib>Wang, Zhong-quan</creatorcontrib><creatorcontrib>Cui, Jing</creatorcontrib><collection>Opposing Viewpoints Resource Center</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Complete (ProQuest Database)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>BMC infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Ze-yu</au><au>Fu, Guang-yu</au><au>Wang, Shan-mei</au><au>Qin, Dong-chun</au><au>Wang, Zhong-quan</au><au>Cui, Jing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid screening for Chlamydia trachomatis infection by detecting [alpha]-mannosidase activity in urogenital tract specimens</atitle><jtitle>BMC infectious diseases</jtitle><date>2013-01-24</date><risdate>2013</risdate><volume>13</volume><issue>1</issue><spage>36</spage><epage>36</epage><pages>36-36</pages><issn>1471-2334</issn><eissn>1471-2334</eissn><abstract>Chlamydia trachomatis may cause multiple different urogenital tract disorders, but current non-culture assays for rapid screening of C. trachomatis typically use immunochromatography-based methods. We established another new rapid non-culture method for detection of C. trachomatis based on the measurement of [alpha]-mannosidase enzymatic activity in urogenital tract specimens. To evaluate the performance of this method, [alpha]-mannosidase activities of C. trachomatis serotype D strain ã and 29 standard strains related to clinical urogenital pathogens were investigated. Furthermore, 553 urogenital tract specimens were used for clinical assays via cell culture method and ligase chain reaction method (LCR), adopting an expanded gold standard. Only C. trachomatis was positive for [alpha]-mannosidase activity among different types of microbes tested in the research. When prostate fluid specimens, which have some interfering activity, were excluded, the sensitivity and specificity of the enzymatic method were 91.8% (78/85) and 98.3% (409/416), respectively. There were no significant differences (P > 0.05). These results showed that [alpha]-mannosidase activity could be utilised as a screening marker of C. trachomatis infection.</abstract><cop>London</cop><pub>BioMed Central Ltd</pub><doi>10.1186/1471-2334-13-36</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Chlamydia Chlamydia infections Chlamydia trachomatis Health aspects Infections Ligases Medical tests Methods Microbiology Sexually transmitted diseases STD Studies Women |
| title | Rapid screening for Chlamydia trachomatis infection by detecting [alpha]-mannosidase activity in urogenital tract specimens |
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