Use of a Mariner-Based Transposon Mutagenesis System To Isolate Clostridium perfringens Mutants Deficient in Gliding Motility

Clostridium perfringens is an anaerobic Gram-positive pathogen that causes many human and animal diseases, including food poisoning and gas gangrene. C. perfringens lacks flagella but possesses type IV pili (TFP). We have previously shown that C. perfringens can glide across an agar surface in long...

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Veröffentlicht in:	Journal of Bacteriology 2013-02, Vol.195 (3), p.629-636
Hauptverfasser:	Liu, Hualan, Bouillaut, Laurent, Sonenshein, Abraham L, Melville, Stephen B
Format:	Artikel
Sprache:	eng
Schlagworte:	agar bacteria Bacterial proteins Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Cells Chromosome Mapping Chromosomes, Bacterial - genetics clones Clostridium perfringens Clostridium perfringens - classification Clostridium perfringens - genetics Clostridium perfringens - physiology Clostridium perfringens - ultrastructure DNA Transposable Elements - genetics DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism fimbriae flagellum foodborne illness Gene Expression Regulation, Bacterial - physiology Gene Expression Regulation, Enzymologic - physiology genes Genetic Complementation Test Gram-positive bacteria Movement - physiology Mutagenesis mutants Mutation pathogens phenotype Plasmids - genetics proteins RNA, Bacterial - genetics RNA, Ribosomal - genetics sequence deletion Transposases - genetics Transposases - metabolism transposons
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