Development of a Real-Time RT-PCR Assay for the Detection of Crimean-Congo Hemorrhagic Fever Virus
Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10–50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreadin...
Gespeichert in:
| Veröffentlicht in: | Vector borne and zoonotic diseases (Larchmont, N.Y.) N.Y.), 2012-09, Vol.12 (9), p.786-793 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 793 |
|---|---|
| container_issue | 9 |
| container_start_page | 786 |
| container_title | Vector borne and zoonotic diseases (Larchmont, N.Y.) |
| container_volume | 12 |
| creator | Atkinson, Barry Chamberlain, John Logue, Christopher H. Cook, Nicola Bruce, Christine Dowall, Stuart D. Hewson, Roger |
| description | Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10–50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreading to new areas with several countries reporting their first human cases of CCHF disease within the past 10 years. We report a novel real-time RT-PCR assay designed to amplify a conserved region of the CCHF virus S segment. It is capable of detecting strains from all 7 groups of CCHF, including the AP92 strain that until recently represented a lineage of strains that were not associated with human disease. The limit of detection of the assay is 5 copies of target RNA, and the assay shows no cross-reactivity with other viruses from within the same genus, or with viruses causing similar human disease. |
| doi_str_mv | 10.1089/vbz.2011.0770 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1315613734</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1039207245</sourcerecordid><originalsourceid>FETCH-LOGICAL-c370t-fe5b1f9b232f99a210fbe667a115850359928b3a760272797360db8ba45e8e933</originalsourceid><addsrcrecordid>eNqF0c1LwzAYBvAgit9Hr5Kjl8x8LE1zlM4vGChjei1J92ZW2mYmnTD_elM2ve6UEH48vG8ehK4YHTGa69tv-zPilLERVYoeoFMmpSJKSX043AUlIsvUCTqL8ZNSznImj9EJ55wppuQpshP4hsavWuh67B02eAamIfO6BTybk9dihu9iNBvsfMD9B-AJ9FD1te8GXYTkTEcK3y09foLWh_BhlnWFH1JswO91WMcLdORME-Fyd56jt4f7efFEpi-Pz8XdlFRC0Z44kJY5bbngTmvDGXUW0uyGMZlLKqTWPLfCqIxyxZVWIqMLm1szlpCDFuIc3WxzV8F_rSH2ZVvHCprGdODXsWSCyYwJJcb7KRWaU8XHMlGypVXwMQZw5SotbcImoXJooEwNlEMD5dBA8te76LVtYfGv_748AbEFw7PpuqYGC6HfE_sLyVGQIg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1039207245</pqid></control><display><type>article</type><title>Development of a Real-Time RT-PCR Assay for the Detection of Crimean-Congo Hemorrhagic Fever Virus</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Atkinson, Barry ; Chamberlain, John ; Logue, Christopher H. ; Cook, Nicola ; Bruce, Christine ; Dowall, Stuart D. ; Hewson, Roger</creator><creatorcontrib>Atkinson, Barry ; Chamberlain, John ; Logue, Christopher H. ; Cook, Nicola ; Bruce, Christine ; Dowall, Stuart D. ; Hewson, Roger</creatorcontrib><description>Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10–50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreading to new areas with several countries reporting their first human cases of CCHF disease within the past 10 years. We report a novel real-time RT-PCR assay designed to amplify a conserved region of the CCHF virus S segment. It is capable of detecting strains from all 7 groups of CCHF, including the AP92 strain that until recently represented a lineage of strains that were not associated with human disease. The limit of detection of the assay is 5 copies of target RNA, and the assay shows no cross-reactivity with other viruses from within the same genus, or with viruses causing similar human disease.</description><identifier>ISSN: 1530-3667</identifier><identifier>EISSN: 1557-7759</identifier><identifier>DOI: 10.1089/vbz.2011.0770</identifier><identifier>PMID: 22217175</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Base Sequence ; Cercopithecus aethiops ; Crimean-Congo hemorrhagic fever virus ; Cross-reactivity ; Disease transmission ; DNA Primers - genetics ; Hemorrhagic fever ; Hemorrhagic Fever Virus, Crimean-Congo - genetics ; Hemorrhagic Fever Virus, Crimean-Congo - isolation & purification ; Hemorrhagic Fever, Crimean - virology ; Hospitals ; Humans ; Molecular Sequence Data ; Original Articles ; Polymerase chain reaction ; Real-Time Polymerase Chain Reaction - methods ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA ; RNA, Viral - genetics ; RNA, Viral - isolation & purification ; Sensitivity and Specificity ; Sequence Alignment ; Species Specificity ; Spreading ; tick-borne diseases ; Vectors ; Vero Cells</subject><ispartof>Vector borne and zoonotic diseases (Larchmont, N.Y.), 2012-09, Vol.12 (9), p.786-793</ispartof><rights>2012, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-fe5b1f9b232f99a210fbe667a115850359928b3a760272797360db8ba45e8e933</citedby><cites>FETCH-LOGICAL-c370t-fe5b1f9b232f99a210fbe667a115850359928b3a760272797360db8ba45e8e933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22217175$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Atkinson, Barry</creatorcontrib><creatorcontrib>Chamberlain, John</creatorcontrib><creatorcontrib>Logue, Christopher H.</creatorcontrib><creatorcontrib>Cook, Nicola</creatorcontrib><creatorcontrib>Bruce, Christine</creatorcontrib><creatorcontrib>Dowall, Stuart D.</creatorcontrib><creatorcontrib>Hewson, Roger</creatorcontrib><title>Development of a Real-Time RT-PCR Assay for the Detection of Crimean-Congo Hemorrhagic Fever Virus</title><title>Vector borne and zoonotic diseases (Larchmont, N.Y.)</title><addtitle>Vector Borne Zoonotic Dis</addtitle><description>Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10–50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreading to new areas with several countries reporting their first human cases of CCHF disease within the past 10 years. We report a novel real-time RT-PCR assay designed to amplify a conserved region of the CCHF virus S segment. It is capable of detecting strains from all 7 groups of CCHF, including the AP92 strain that until recently represented a lineage of strains that were not associated with human disease. The limit of detection of the assay is 5 copies of target RNA, and the assay shows no cross-reactivity with other viruses from within the same genus, or with viruses causing similar human disease.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Cercopithecus aethiops</subject><subject>Crimean-Congo hemorrhagic fever virus</subject><subject>Cross-reactivity</subject><subject>Disease transmission</subject><subject>DNA Primers - genetics</subject><subject>Hemorrhagic fever</subject><subject>Hemorrhagic Fever Virus, Crimean-Congo - genetics</subject><subject>Hemorrhagic Fever Virus, Crimean-Congo - isolation & purification</subject><subject>Hemorrhagic Fever, Crimean - virology</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Original Articles</subject><subject>Polymerase chain reaction</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA</subject><subject>RNA, Viral - genetics</subject><subject>RNA, Viral - isolation & purification</subject><subject>Sensitivity and Specificity</subject><subject>Sequence Alignment</subject><subject>Species Specificity</subject><subject>Spreading</subject><subject>tick-borne diseases</subject><subject>Vectors</subject><subject>Vero Cells</subject><issn>1530-3667</issn><issn>1557-7759</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c1LwzAYBvAgit9Hr5Kjl8x8LE1zlM4vGChjei1J92ZW2mYmnTD_elM2ve6UEH48vG8ehK4YHTGa69tv-zPilLERVYoeoFMmpSJKSX043AUlIsvUCTqL8ZNSznImj9EJ55wppuQpshP4hsavWuh67B02eAamIfO6BTybk9dihu9iNBvsfMD9B-AJ9FD1te8GXYTkTEcK3y09foLWh_BhlnWFH1JswO91WMcLdORME-Fyd56jt4f7efFEpi-Pz8XdlFRC0Z44kJY5bbngTmvDGXUW0uyGMZlLKqTWPLfCqIxyxZVWIqMLm1szlpCDFuIc3WxzV8F_rSH2ZVvHCprGdODXsWSCyYwJJcb7KRWaU8XHMlGypVXwMQZw5SotbcImoXJooEwNlEMD5dBA8te76LVtYfGv_748AbEFw7PpuqYGC6HfE_sLyVGQIg</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Atkinson, Barry</creator><creator>Chamberlain, John</creator><creator>Logue, Christopher H.</creator><creator>Cook, Nicola</creator><creator>Bruce, Christine</creator><creator>Dowall, Stuart D.</creator><creator>Hewson, Roger</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20120901</creationdate><title>Development of a Real-Time RT-PCR Assay for the Detection of Crimean-Congo Hemorrhagic Fever Virus</title><author>Atkinson, Barry ; Chamberlain, John ; Logue, Christopher H. ; Cook, Nicola ; Bruce, Christine ; Dowall, Stuart D. ; Hewson, Roger</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-fe5b1f9b232f99a210fbe667a115850359928b3a760272797360db8ba45e8e933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Cercopithecus aethiops</topic><topic>Crimean-Congo hemorrhagic fever virus</topic><topic>Cross-reactivity</topic><topic>Disease transmission</topic><topic>DNA Primers - genetics</topic><topic>Hemorrhagic fever</topic><topic>Hemorrhagic Fever Virus, Crimean-Congo - genetics</topic><topic>Hemorrhagic Fever Virus, Crimean-Congo - isolation & purification</topic><topic>Hemorrhagic Fever, Crimean - virology</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Original Articles</topic><topic>Polymerase chain reaction</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA</topic><topic>RNA, Viral - genetics</topic><topic>RNA, Viral - isolation & purification</topic><topic>Sensitivity and Specificity</topic><topic>Sequence Alignment</topic><topic>Species Specificity</topic><topic>Spreading</topic><topic>tick-borne diseases</topic><topic>Vectors</topic><topic>Vero Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Atkinson, Barry</creatorcontrib><creatorcontrib>Chamberlain, John</creatorcontrib><creatorcontrib>Logue, Christopher H.</creatorcontrib><creatorcontrib>Cook, Nicola</creatorcontrib><creatorcontrib>Bruce, Christine</creatorcontrib><creatorcontrib>Dowall, Stuart D.</creatorcontrib><creatorcontrib>Hewson, Roger</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Atkinson, Barry</au><au>Chamberlain, John</au><au>Logue, Christopher H.</au><au>Cook, Nicola</au><au>Bruce, Christine</au><au>Dowall, Stuart D.</au><au>Hewson, Roger</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a Real-Time RT-PCR Assay for the Detection of Crimean-Congo Hemorrhagic Fever Virus</atitle><jtitle>Vector borne and zoonotic diseases (Larchmont, N.Y.)</jtitle><addtitle>Vector Borne Zoonotic Dis</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>12</volume><issue>9</issue><spage>786</spage><epage>793</epage><pages>786-793</pages><issn>1530-3667</issn><eissn>1557-7759</eissn><abstract>Crimean-Congo hemorrhagic fever (CCHF) is a virulent tick-borne disease with a case fatality rate ranging from 10–50% for tick-borne transmission, and up to 80% for nosocomial transmission. Human cases have been reported in over 30 countries across Europe, Asia, and Africa. It appears to be spreading to new areas with several countries reporting their first human cases of CCHF disease within the past 10 years. We report a novel real-time RT-PCR assay designed to amplify a conserved region of the CCHF virus S segment. It is capable of detecting strains from all 7 groups of CCHF, including the AP92 strain that until recently represented a lineage of strains that were not associated with human disease. The limit of detection of the assay is 5 copies of target RNA, and the assay shows no cross-reactivity with other viruses from within the same genus, or with viruses causing similar human disease.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>22217175</pmid><doi>10.1089/vbz.2011.0770</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1530-3667 |
| ispartof | Vector borne and zoonotic diseases (Larchmont, N.Y.), 2012-09, Vol.12 (9), p.786-793 |
| issn | 1530-3667 1557-7759 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1315613734 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Base Sequence Cercopithecus aethiops Crimean-Congo hemorrhagic fever virus Cross-reactivity Disease transmission DNA Primers - genetics Hemorrhagic fever Hemorrhagic Fever Virus, Crimean-Congo - genetics Hemorrhagic Fever Virus, Crimean-Congo - isolation & purification Hemorrhagic Fever, Crimean - virology Hospitals Humans Molecular Sequence Data Original Articles Polymerase chain reaction Real-Time Polymerase Chain Reaction - methods Reverse Transcriptase Polymerase Chain Reaction - methods RNA RNA, Viral - genetics RNA, Viral - isolation & purification Sensitivity and Specificity Sequence Alignment Species Specificity Spreading tick-borne diseases Vectors Vero Cells |
| title | Development of a Real-Time RT-PCR Assay for the Detection of Crimean-Congo Hemorrhagic Fever Virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T20%3A41%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20Real-Time%20RT-PCR%20Assay%20for%20the%20Detection%20of%20Crimean-Congo%20Hemorrhagic%20Fever%20Virus&rft.jtitle=Vector%20borne%20and%20zoonotic%20diseases%20(Larchmont,%20N.Y.)&rft.au=Atkinson,%20Barry&rft.date=2012-09-01&rft.volume=12&rft.issue=9&rft.spage=786&rft.epage=793&rft.pages=786-793&rft.issn=1530-3667&rft.eissn=1557-7759&rft_id=info:doi/10.1089/vbz.2011.0770&rft_dat=%3Cproquest_cross%3E1039207245%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1039207245&rft_id=info:pmid/22217175&rfr_iscdi=true |