Enzymatic and genetic characterization of the DasD protein possessing N-acetyl-β-d-glucosaminidase activity in Streptomyces coelicolor A3(2)

Abstract The dasD gene is located just downstream of the dasABC gene cluster, encoding components of an ABC transporter for uptake of a chitin-degradation product N,N′-diacetylchitobiose [(GlcNAc)2] in Streptomyces coelicolor A3(2). To clarify the roles of the DasD protein in the degradation and ass...

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Veröffentlicht in:	FEMS microbiology letters 2013-03, Vol.340 (1), p.33-40
Hauptverfasser:	Saito, Akihiro, Ebise, Hiroki, Orihara, Yukari, Murakami, Satoshi, Sano, Yukari, Kimura, Akane, Sugiyama, Yuuta, Ando, Akikazu, Fujii, Takeshi, Miyashita, Kiyotaka
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylglucosaminidase - genetics Acetylglucosaminidase - metabolism actinomycetes Bacteriology Biological and medical sciences Chitin - metabolism chitinase chitobiase Cloning, Molecular Cytoplasm - enzymology Enzyme Stability Escherichia coli Fundamental and applied biological sciences. Psychology Gene Deletion Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic GH family 3 glycoside hydrolase Hydrogen-Ion Concentration Microbiology Miscellaneous Recombinant Proteins - genetics Recombinant Proteins - metabolism Streptomyces coelicolor Streptomyces coelicolor - enzymology Streptomyces coelicolor - genetics Streptomyces coelicolor - metabolism Temperature
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