Application of next generation qPCR and sequencing platforms to mRNA biomarker analysis

Recent years have seen the emergence of new high-throughput PCR and sequencing platforms with the potential to bring analysis of transcriptional biomarkers to a broader range of clinical applications and to provide increasing depth to our understanding of the transcriptome. We present an overview of...

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2013-01, Vol.59 (1), p.89-100
Hauptverfasser:	Devonshire, Alison S., Sanders, Rebecca, Wilkes, Timothy M., Taylor, Martin S., Foy, Carole A., Huggett, Jim F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bioinformatics biomarkers Biomarkers - metabolism Digital PCR DNA Cleavage Gene expression Gene Library High-Throughput Nucleotide Sequencing - standards Humans Microfluidic Analytical Techniques mRNA biomarker Nanofluidic PCR Next generation sequencing NGS Polymerase chain reaction Real-Time Polymerase Chain Reaction - standards Reference Standards Reverse Transcriptase Polymerase Chain Reaction - standards Reviews RNA, Messenger - genetics RNA, Messenger - isolation & purification RNA, Messenger - metabolism RNA-Seq Therapeutic applications Transcription
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