Temperature response of carbon isotope discrimination and mesophyll conductance in tobacco
ABSTRACT The partial pressure of CO2 at the sites of carboxylation within chloroplasts depends on the conductance to CO2 diffusion from intercellular airspace to the sites of carboxylation, termed mesophyll conductance (gm). We investigated the temperature response of gm in tobacco (Nicotiana tabacu...
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| creator | EVANS, JOHN R. VON CAEMMERER, SUSANNE |
| description | ABSTRACT
The partial pressure of CO2 at the sites of carboxylation within chloroplasts depends on the conductance to CO2 diffusion from intercellular airspace to the sites of carboxylation, termed mesophyll conductance (gm). We investigated the temperature response of gm in tobacco (Nicotiana tabacum) by combining gas exchange in high light, ambient CO2 in either 2 or 21% O2 with carbon isotope measurements using tuneable diode laser spectroscopy. The gm increased linearly with temperature in 2 or 21% O2. In 21% O2, isotope discrimination associated with gm decreased from 5.0 ± 0.2 to 1.8 ± 0.2‰ as temperature increased from 15 to 40 °C, but the photorespiratory contribution to the isotopic signal is significant. While the fractionation factor for photorespiration (f = 16.2 ± 0.7‰) was independent of temperature between 20 and 35 °C, discrimination associated with photorespiration increased from 1.1 ± 0.01 to 2.7 ± 0.02‰ from 15 to 40 °C. Other mitochondrial respiration contributed around 0.2 ± 0.03‰. The drawdown in CO2 partial pressure from ambient air to intercellular airspaces was nearly independent of leaf temperature. By contrast, the increase in gm with increasing leaf temperature resulted in the drawdown in CO2 partial pressure between intercellular airspaces and the sites of carboxylation decreasing substantially at high temperature.
A linear relationship between mesophyll conductance and temperature (15–40°C) was observed in tobacco leaves using carbon isotope discrimination measurements. Photorespiration made a significant contribution to the discrimination signal, contributing more than mesophyll conductance at 40°C. The increase in mesophyll conductance with increasing leaf temperature resulted in the drawdown in CO2 partial pressure between intercellular airspaces and the sites of carboxylation decreasing substantially at high temperature. |
| doi_str_mv | 10.1111/j.1365-3040.2012.02591.x |
| format | Article |
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The partial pressure of CO2 at the sites of carboxylation within chloroplasts depends on the conductance to CO2 diffusion from intercellular airspace to the sites of carboxylation, termed mesophyll conductance (gm). We investigated the temperature response of gm in tobacco (Nicotiana tabacum) by combining gas exchange in high light, ambient CO2 in either 2 or 21% O2 with carbon isotope measurements using tuneable diode laser spectroscopy. The gm increased linearly with temperature in 2 or 21% O2. In 21% O2, isotope discrimination associated with gm decreased from 5.0 ± 0.2 to 1.8 ± 0.2‰ as temperature increased from 15 to 40 °C, but the photorespiratory contribution to the isotopic signal is significant. While the fractionation factor for photorespiration (f = 16.2 ± 0.7‰) was independent of temperature between 20 and 35 °C, discrimination associated with photorespiration increased from 1.1 ± 0.01 to 2.7 ± 0.02‰ from 15 to 40 °C. Other mitochondrial respiration contributed around 0.2 ± 0.03‰. The drawdown in CO2 partial pressure from ambient air to intercellular airspaces was nearly independent of leaf temperature. By contrast, the increase in gm with increasing leaf temperature resulted in the drawdown in CO2 partial pressure between intercellular airspaces and the sites of carboxylation decreasing substantially at high temperature.
A linear relationship between mesophyll conductance and temperature (15–40°C) was observed in tobacco leaves using carbon isotope discrimination measurements. Photorespiration made a significant contribution to the discrimination signal, contributing more than mesophyll conductance at 40°C. The increase in mesophyll conductance with increasing leaf temperature resulted in the drawdown in CO2 partial pressure between intercellular airspaces and the sites of carboxylation decreasing substantially at high temperature.</description><identifier>ISSN: 0140-7791</identifier><identifier>EISSN: 1365-3040</identifier><identifier>DOI: 10.1111/j.1365-3040.2012.02591.x</identifier><identifier>PMID: 22882584</identifier><identifier>CODEN: PLCEDV</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Biological Transport ; Carbon Dioxide - metabolism ; Carbon Isotopes - analysis ; carbon reactions ; Cell Respiration ; Chloroplasts - metabolism ; CO2 ; Fundamental and applied biological sciences. Psychology ; heat ; Light ; Mesophyll Cells - metabolism ; Models, Theoretical ; Nicotiana - metabolism ; Nicotiana - physiology ; Nicotiana - radiation effects ; Partial Pressure ; photorespiration ; photosynthesis ; Photosynthesis - physiology ; Plant Leaves - metabolism ; Plant Leaves - physiology ; Plant Leaves - radiation effects ; Plant Stomata - metabolism ; Plant Stomata - physiology ; Plant Stomata - radiation effects ; Plant Transpiration - physiology ; Ribulose-Bisphosphate Carboxylase - metabolism ; Temperature</subject><ispartof>Plant, cell and environment, 2013-04, Vol.36 (4), p.745-756</ispartof><rights>2012 Blackwell Publishing Ltd</rights><rights>2014 INIST-CNRS</rights><rights>2012 Blackwell Publishing Ltd.</rights><rights>Copyright © 2013 Blackwell Publishing Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5431-923aacd57652742f21ea76997d6dd6f14fd8223e60aea1db5e6766d0d435207d3</citedby><cites>FETCH-LOGICAL-c5431-923aacd57652742f21ea76997d6dd6f14fd8223e60aea1db5e6766d0d435207d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-3040.2012.02591.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-3040.2012.02591.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27081673$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22882584$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>EVANS, JOHN R.</creatorcontrib><creatorcontrib>VON CAEMMERER, SUSANNE</creatorcontrib><title>Temperature response of carbon isotope discrimination and mesophyll conductance in tobacco</title><title>Plant, cell and environment</title><addtitle>Plant Cell Environ</addtitle><description>ABSTRACT
The partial pressure of CO2 at the sites of carboxylation within chloroplasts depends on the conductance to CO2 diffusion from intercellular airspace to the sites of carboxylation, termed mesophyll conductance (gm). We investigated the temperature response of gm in tobacco (Nicotiana tabacum) by combining gas exchange in high light, ambient CO2 in either 2 or 21% O2 with carbon isotope measurements using tuneable diode laser spectroscopy. The gm increased linearly with temperature in 2 or 21% O2. In 21% O2, isotope discrimination associated with gm decreased from 5.0 ± 0.2 to 1.8 ± 0.2‰ as temperature increased from 15 to 40 °C, but the photorespiratory contribution to the isotopic signal is significant. While the fractionation factor for photorespiration (f = 16.2 ± 0.7‰) was independent of temperature between 20 and 35 °C, discrimination associated with photorespiration increased from 1.1 ± 0.01 to 2.7 ± 0.02‰ from 15 to 40 °C. Other mitochondrial respiration contributed around 0.2 ± 0.03‰. The drawdown in CO2 partial pressure from ambient air to intercellular airspaces was nearly independent of leaf temperature. By contrast, the increase in gm with increasing leaf temperature resulted in the drawdown in CO2 partial pressure between intercellular airspaces and the sites of carboxylation decreasing substantially at high temperature.
A linear relationship between mesophyll conductance and temperature (15–40°C) was observed in tobacco leaves using carbon isotope discrimination measurements. Photorespiration made a significant contribution to the discrimination signal, contributing more than mesophyll conductance at 40°C. The increase in mesophyll conductance with increasing leaf temperature resulted in the drawdown in CO2 partial pressure between intercellular airspaces and the sites of carboxylation decreasing substantially at high temperature.</description><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Carbon Dioxide - metabolism</subject><subject>Carbon Isotopes - analysis</subject><subject>carbon reactions</subject><subject>Cell Respiration</subject><subject>Chloroplasts - metabolism</subject><subject>CO2</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>heat</subject><subject>Light</subject><subject>Mesophyll Cells - metabolism</subject><subject>Models, Theoretical</subject><subject>Nicotiana - metabolism</subject><subject>Nicotiana - physiology</subject><subject>Nicotiana - radiation effects</subject><subject>Partial Pressure</subject><subject>photorespiration</subject><subject>photosynthesis</subject><subject>Photosynthesis - physiology</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Leaves - physiology</subject><subject>Plant Leaves - radiation effects</subject><subject>Plant Stomata - metabolism</subject><subject>Plant Stomata - physiology</subject><subject>Plant Stomata - radiation effects</subject><subject>Plant Transpiration - physiology</subject><subject>Ribulose-Bisphosphate Carboxylase - metabolism</subject><subject>Temperature</subject><issn>0140-7791</issn><issn>1365-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1u1DAURi0EotPCKyBLCIlNUv87WbBAo1KQKpVFu-nG8tg3IqPEDnaidt4ehxmKxApvbF2fe_35IIQpqWlZl_uaciUrTgSpGaGsJky2tH56gTbPFy_RhlBBKq1beobOc94TUgq6fY3OGGsaJhuxQQ93ME6Q7LwkwAnyFEMGHDvsbNrFgPsc5zgB9n12qR_7YOe-lG3weIQcpx-HYcAuBr-42QYHuA94jjvrXHyDXnV2yPD2tF-g-y9Xd9uv1c3t9bft55vKScFp1TJurfNSK8m0YB2jYLVqW-2V96qjovMNYxwUsWCp30lQWilPvOCSEe35Bfp4nDul-HOBPJuxhIVhsAHikg3lVDRt0aIK-v4fdB-XFEo6QwVnjDSS6EI1R8qlmHOCzkzl6zYdDCVm9W_2ZtVsVs1m9W9--zdPpfXd6YFlN4J_bvwjvAAfToDNzg5dKtL6_JfTpKFK88J9OnKP_QCH_w5gvm-v1hP_BbAFoGo</recordid><startdate>201304</startdate><enddate>201304</enddate><creator>EVANS, JOHN R.</creator><creator>VON CAEMMERER, SUSANNE</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201304</creationdate><title>Temperature response of carbon isotope discrimination and mesophyll conductance in tobacco</title><author>EVANS, JOHN R. ; VON CAEMMERER, SUSANNE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5431-923aacd57652742f21ea76997d6dd6f14fd8223e60aea1db5e6766d0d435207d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Carbon Dioxide - metabolism</topic><topic>Carbon Isotopes - analysis</topic><topic>carbon reactions</topic><topic>Cell Respiration</topic><topic>Chloroplasts - metabolism</topic><topic>CO2</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>heat</topic><topic>Light</topic><topic>Mesophyll Cells - metabolism</topic><topic>Models, Theoretical</topic><topic>Nicotiana - metabolism</topic><topic>Nicotiana - physiology</topic><topic>Nicotiana - radiation effects</topic><topic>Partial Pressure</topic><topic>photorespiration</topic><topic>photosynthesis</topic><topic>Photosynthesis - physiology</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Leaves - physiology</topic><topic>Plant Leaves - radiation effects</topic><topic>Plant Stomata - metabolism</topic><topic>Plant Stomata - physiology</topic><topic>Plant Stomata - radiation effects</topic><topic>Plant Transpiration - physiology</topic><topic>Ribulose-Bisphosphate Carboxylase - metabolism</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>EVANS, JOHN R.</creatorcontrib><creatorcontrib>VON CAEMMERER, SUSANNE</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant, cell and environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>EVANS, JOHN R.</au><au>VON CAEMMERER, SUSANNE</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temperature response of carbon isotope discrimination and mesophyll conductance in tobacco</atitle><jtitle>Plant, cell and environment</jtitle><addtitle>Plant Cell Environ</addtitle><date>2013-04</date><risdate>2013</risdate><volume>36</volume><issue>4</issue><spage>745</spage><epage>756</epage><pages>745-756</pages><issn>0140-7791</issn><eissn>1365-3040</eissn><coden>PLCEDV</coden><abstract>ABSTRACT
The partial pressure of CO2 at the sites of carboxylation within chloroplasts depends on the conductance to CO2 diffusion from intercellular airspace to the sites of carboxylation, termed mesophyll conductance (gm). We investigated the temperature response of gm in tobacco (Nicotiana tabacum) by combining gas exchange in high light, ambient CO2 in either 2 or 21% O2 with carbon isotope measurements using tuneable diode laser spectroscopy. The gm increased linearly with temperature in 2 or 21% O2. In 21% O2, isotope discrimination associated with gm decreased from 5.0 ± 0.2 to 1.8 ± 0.2‰ as temperature increased from 15 to 40 °C, but the photorespiratory contribution to the isotopic signal is significant. While the fractionation factor for photorespiration (f = 16.2 ± 0.7‰) was independent of temperature between 20 and 35 °C, discrimination associated with photorespiration increased from 1.1 ± 0.01 to 2.7 ± 0.02‰ from 15 to 40 °C. Other mitochondrial respiration contributed around 0.2 ± 0.03‰. The drawdown in CO2 partial pressure from ambient air to intercellular airspaces was nearly independent of leaf temperature. By contrast, the increase in gm with increasing leaf temperature resulted in the drawdown in CO2 partial pressure between intercellular airspaces and the sites of carboxylation decreasing substantially at high temperature.
A linear relationship between mesophyll conductance and temperature (15–40°C) was observed in tobacco leaves using carbon isotope discrimination measurements. Photorespiration made a significant contribution to the discrimination signal, contributing more than mesophyll conductance at 40°C. The increase in mesophyll conductance with increasing leaf temperature resulted in the drawdown in CO2 partial pressure between intercellular airspaces and the sites of carboxylation decreasing substantially at high temperature.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>22882584</pmid><doi>10.1111/j.1365-3040.2012.02591.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Biological Transport Carbon Dioxide - metabolism Carbon Isotopes - analysis carbon reactions Cell Respiration Chloroplasts - metabolism CO2 Fundamental and applied biological sciences. Psychology heat Light Mesophyll Cells - metabolism Models, Theoretical Nicotiana - metabolism Nicotiana - physiology Nicotiana - radiation effects Partial Pressure photorespiration photosynthesis Photosynthesis - physiology Plant Leaves - metabolism Plant Leaves - physiology Plant Leaves - radiation effects Plant Stomata - metabolism Plant Stomata - physiology Plant Stomata - radiation effects Plant Transpiration - physiology Ribulose-Bisphosphate Carboxylase - metabolism Temperature |
| title | Temperature response of carbon isotope discrimination and mesophyll conductance in tobacco |
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