Effects of garlic on Schistosoma mansoni harbored in albino mice: Molecular characterization of the host and parasite
Garlic has been used for its health benefits for thousands of years. Modern research confirmed many of the healing properties of garlic, including its antiparasitic activity. This study was designed to evaluate the antischistosomal action of garlic through detecting the changes in DNA profile of Sch...
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| Veröffentlicht in: | Gene 2013-04, Vol.518 (2), p.287-291 |
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| creator | Riad, Nahed H.A. Taha, Hoda A. Mahmoud, Yomna I. |
| description | Garlic has been used for its health benefits for thousands of years. Modern research confirmed many of the healing properties of garlic, including its antiparasitic activity. This study was designed to evaluate the antischistosomal action of garlic through detecting the changes in DNA profile of Schistosoma mansoni worms and the infected mouse. Forty mice were subcutaneously infected with ~200 Schistosoma mansoni cercariae/mouse. Infected mice were divided into four equal groups: non-treated, prophylactic, therapeutic, and continuously-treated. Non-infected control and garlic-treated groups were assigned for the sake of comparison. Garlic extract (50mg/kg bw/mouse) was given orally, day after day, at a fixed daytime. Seven weeks post-infection, adult schistosomes were recovered by perfusion and the livers of the mice were excised out and were processed for DNA extraction and Random Amplification of Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). The results showed that garlic exerted no major changes in the genome of schistosomes. Nevertheless, that schistosomal infection induced genetic alterations in the DNA of mice, and garlic was able to ameliorate such alterations to a great extent. |
| doi_str_mv | 10.1016/j.gene.2013.01.023 |
| format | Article |
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Modern research confirmed many of the healing properties of garlic, including its antiparasitic activity. This study was designed to evaluate the antischistosomal action of garlic through detecting the changes in DNA profile of Schistosoma mansoni worms and the infected mouse. Forty mice were subcutaneously infected with ~200 Schistosoma mansoni cercariae/mouse. Infected mice were divided into four equal groups: non-treated, prophylactic, therapeutic, and continuously-treated. Non-infected control and garlic-treated groups were assigned for the sake of comparison. Garlic extract (50mg/kg bw/mouse) was given orally, day after day, at a fixed daytime. Seven weeks post-infection, adult schistosomes were recovered by perfusion and the livers of the mice were excised out and were processed for DNA extraction and Random Amplification of Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). The results showed that garlic exerted no major changes in the genome of schistosomes. Nevertheless, that schistosomal infection induced genetic alterations in the DNA of mice, and garlic was able to ameliorate such alterations to a great extent.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/j.gene.2013.01.023</identifier><identifier>PMID: 23414971</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>adults ; Animals ; antiparasitic agents ; cercariae ; DNA ; DNA, Protozoan - analysis ; DNA, Protozoan - genetics ; Garlic ; Gene Expression Profiling ; genome ; Liver - parasitology ; Male ; Mice ; Mice - parasitology ; Parasite Egg Count ; parasites ; Phytotherapy ; Plant Extracts - pharmacology ; Plant Extracts - therapeutic use ; random amplified polymorphic DNA technique ; RAPD-PCR ; Schistosoma mansoni ; Schistosoma mansoni - drug effects ; Schistosoma mansoni - genetics ; Schistosomiasis mansoni - drug therapy ; Schistosomiasis mansoni - parasitology ; Schistosomicides - pharmacology ; Schistosomicides - therapeutic use</subject><ispartof>Gene, 2013-04, Vol.518 (2), p.287-291</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-e5eb2094442d8c10e5678706997259756cc549470b28eaa2c5f493cde47bdef23</citedby><cites>FETCH-LOGICAL-c380t-e5eb2094442d8c10e5678706997259756cc549470b28eaa2c5f493cde47bdef23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.gene.2013.01.023$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23414971$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Riad, Nahed H.A.</creatorcontrib><creatorcontrib>Taha, Hoda A.</creatorcontrib><creatorcontrib>Mahmoud, Yomna I.</creatorcontrib><title>Effects of garlic on Schistosoma mansoni harbored in albino mice: Molecular characterization of the host and parasite</title><title>Gene</title><addtitle>Gene</addtitle><description>Garlic has been used for its health benefits for thousands of years. Modern research confirmed many of the healing properties of garlic, including its antiparasitic activity. This study was designed to evaluate the antischistosomal action of garlic through detecting the changes in DNA profile of Schistosoma mansoni worms and the infected mouse. Forty mice were subcutaneously infected with ~200 Schistosoma mansoni cercariae/mouse. Infected mice were divided into four equal groups: non-treated, prophylactic, therapeutic, and continuously-treated. Non-infected control and garlic-treated groups were assigned for the sake of comparison. Garlic extract (50mg/kg bw/mouse) was given orally, day after day, at a fixed daytime. Seven weeks post-infection, adult schistosomes were recovered by perfusion and the livers of the mice were excised out and were processed for DNA extraction and Random Amplification of Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). The results showed that garlic exerted no major changes in the genome of schistosomes. Nevertheless, that schistosomal infection induced genetic alterations in the DNA of mice, and garlic was able to ameliorate such alterations to a great extent.</description><subject>adults</subject><subject>Animals</subject><subject>antiparasitic agents</subject><subject>cercariae</subject><subject>DNA</subject><subject>DNA, Protozoan - analysis</subject><subject>DNA, Protozoan - genetics</subject><subject>Garlic</subject><subject>Gene Expression Profiling</subject><subject>genome</subject><subject>Liver - parasitology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice - parasitology</subject><subject>Parasite Egg Count</subject><subject>parasites</subject><subject>Phytotherapy</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Extracts - therapeutic use</subject><subject>random amplified polymorphic DNA technique</subject><subject>RAPD-PCR</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - drug effects</subject><subject>Schistosoma mansoni - genetics</subject><subject>Schistosomiasis mansoni - drug therapy</subject><subject>Schistosomiasis mansoni - parasitology</subject><subject>Schistosomicides - pharmacology</subject><subject>Schistosomicides - therapeutic use</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAURi1ERYeBP8ACvGST4GccIzaoKg-pVRdt15bj3Mx4lNiD7SDBr8ejKSyxF174u-fa5yL0hpKWEtp9OLQ7CNAyQnlLaEsYf4Y2tFe6IYT3z9GGcNU3lFJ9iV7mfCB1ScleoEvGBRVa0Q1ar6cJXMk4Tnhn0-wdjgHfu73PJea4WLzYkGPweG_TEBOM2Ads58GHiBfv4CO-jTO4dbYJu5qxrkDyv23xlVOhZQ94H3PBNoz4WO-zL_AKXUx2zvD66dyixy_XD1ffmpu7r9-vPt80jvekNCBhYEQLIdjYO0pAdqpXpNNaMamV7JyTQgtFBtaDtczJSWjuRhBqGGFifIven7nHFH-skItZfHYwzzZAXLOhnApFua57i9g56lLMOcFkjskvNv0ylJiTbnMwJ93mpNsQaqruWvT2ib8OC4z_Sv76rYF358Bko7G75LN5vK8EWUdRf9Wd-n46J6B6-Okhmew8BAejT3UwZoz-fy_4A4sumqg</recordid><startdate>20130415</startdate><enddate>20130415</enddate><creator>Riad, Nahed H.A.</creator><creator>Taha, Hoda A.</creator><creator>Mahmoud, Yomna I.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130415</creationdate><title>Effects of garlic on Schistosoma mansoni harbored in albino mice: Molecular characterization of the host and parasite</title><author>Riad, Nahed H.A. ; Taha, Hoda A. ; Mahmoud, Yomna I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-e5eb2094442d8c10e5678706997259756cc549470b28eaa2c5f493cde47bdef23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>adults</topic><topic>Animals</topic><topic>antiparasitic agents</topic><topic>cercariae</topic><topic>DNA</topic><topic>DNA, Protozoan - analysis</topic><topic>DNA, Protozoan - genetics</topic><topic>Garlic</topic><topic>Gene Expression Profiling</topic><topic>genome</topic><topic>Liver - parasitology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice - parasitology</topic><topic>Parasite Egg Count</topic><topic>parasites</topic><topic>Phytotherapy</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Extracts - therapeutic use</topic><topic>random amplified polymorphic DNA technique</topic><topic>RAPD-PCR</topic><topic>Schistosoma mansoni</topic><topic>Schistosoma mansoni - drug effects</topic><topic>Schistosoma mansoni - genetics</topic><topic>Schistosomiasis mansoni - drug therapy</topic><topic>Schistosomiasis mansoni - parasitology</topic><topic>Schistosomicides - pharmacology</topic><topic>Schistosomicides - therapeutic use</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Riad, Nahed H.A.</creatorcontrib><creatorcontrib>Taha, Hoda A.</creatorcontrib><creatorcontrib>Mahmoud, Yomna I.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Riad, Nahed H.A.</au><au>Taha, Hoda A.</au><au>Mahmoud, Yomna I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of garlic on Schistosoma mansoni harbored in albino mice: Molecular characterization of the host and parasite</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>2013-04-15</date><risdate>2013</risdate><volume>518</volume><issue>2</issue><spage>287</spage><epage>291</epage><pages>287-291</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Garlic has been used for its health benefits for thousands of years. Modern research confirmed many of the healing properties of garlic, including its antiparasitic activity. This study was designed to evaluate the antischistosomal action of garlic through detecting the changes in DNA profile of Schistosoma mansoni worms and the infected mouse. Forty mice were subcutaneously infected with ~200 Schistosoma mansoni cercariae/mouse. Infected mice were divided into four equal groups: non-treated, prophylactic, therapeutic, and continuously-treated. Non-infected control and garlic-treated groups were assigned for the sake of comparison. Garlic extract (50mg/kg bw/mouse) was given orally, day after day, at a fixed daytime. Seven weeks post-infection, adult schistosomes were recovered by perfusion and the livers of the mice were excised out and were processed for DNA extraction and Random Amplification of Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). The results showed that garlic exerted no major changes in the genome of schistosomes. Nevertheless, that schistosomal infection induced genetic alterations in the DNA of mice, and garlic was able to ameliorate such alterations to a great extent.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23414971</pmid><doi>10.1016/j.gene.2013.01.023</doi><tpages>5</tpages></addata></record> |
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| subjects | adults Animals antiparasitic agents cercariae DNA DNA, Protozoan - analysis DNA, Protozoan - genetics Garlic Gene Expression Profiling genome Liver - parasitology Male Mice Mice - parasitology Parasite Egg Count parasites Phytotherapy Plant Extracts - pharmacology Plant Extracts - therapeutic use random amplified polymorphic DNA technique RAPD-PCR Schistosoma mansoni Schistosoma mansoni - drug effects Schistosoma mansoni - genetics Schistosomiasis mansoni - drug therapy Schistosomiasis mansoni - parasitology Schistosomicides - pharmacology Schistosomicides - therapeutic use |
| title | Effects of garlic on Schistosoma mansoni harbored in albino mice: Molecular characterization of the host and parasite |
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