Development of a Scale-Down Model of hydrodynamic stress to study the performance of an industrial CHO cell line under simulated production scale bioreactor conditions

► We developed a Scale-Down Model of hydrodynamic stress in large scale bioreactors. ► At high and oscillating hydrodynamic stress, specific productivity was reduced. ► Metabolite consumption, byproduct formation or product quality were not affected. ► DNA damage and repair mechanisms were upregulat...

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Veröffentlicht in:	Journal of biotechnology 2013-03, Vol.164 (1), p.41-49
Hauptverfasser:	Sieck, Jochen B., Cordes, Thekla, Budach, Wolfgang E., Rhiel, Martin H., Suemeghy, Zoltan, Leist, Christian, Villiger, Thomas K., Morbidelli, Massimo, Soos, Miroslav
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bioengineering - methods Bioreactors Biotechnology - methods CHO Cells Computational Biology Cricetinae DNA repair energy Fed-batch cultivation Gene Expression Profiling Hydrodynamics Local energy dissipation rate messenger RNA metabolites microarray technology Models, Theoretical monoclonal antibodies mRNA microarrays Oligonucleotide Array Sequence Analysis product quality RNA, Messenger - analysis Scale-Down Model Stress, Physiological - physiology Transcriptome Transcriptome analysis transcriptomics
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