Purification and characterisation of polyphenol oxidase (PPO) from eggplant (Solanum melongena)

► Eggplant was found to be a very rich source of polyphenol oxidase (PPO). ► Native PPO was 259-fold purified using standard chromatographic procedures. ► The PPO was found to be a 112kDa homodimer. ► The enzyme showed very low Km and high catalytic efficiency with 4-methyl catechol. ► GRAS compound...

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Veröffentlicht in:	Food chemistry 2012-10, Vol.134 (4), p.1855-1861
Hauptverfasser:	Mishra, Bibhuti B., Gautam, Satyendra, Sharma, Arun
Format:	Artikel
Sprache:	eng
Schlagworte:	ascorbic acid Biological and medical sciences catechol catechol oxidase Catechol Oxidase - chemistry Catechol Oxidase - isolation & purification Catechol Oxidase - metabolism Chromatography citric acid cysteine Dimerization eggplants enzymatic browning Enzyme Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology Inhibitor Kinetics kojic acid Methyl catechol Plant Proteins - chemistry Plant Proteins - isolation & purification Plant Proteins - metabolism Polyphenol oxidase (PPO) potassium sodium azide Solanum melongena Solanum melongena - chemistry Solanum melongena - enzymology Substrate Specificity
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