Optogenetic protein clustering and signaling activation in mammalian cells

A modular optogenetic method for higher-order protein oligomerization uses a single cryptochrome 2-based fusion for rapid, reversible and tunable oligomerization in response to blue light. Inducible aggregation can be used to specifically activate different signaling pathways. We report an optogenet...

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Veröffentlicht in:	Nature methods 2013-03, Vol.10 (3), p.249-252
Hauptverfasser:	Bugaj, Lukasz J, Choksi, Atri T, Mesuda, Colin K, Kane, Ravi S, Schaffer, David V
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2253 631/80/86 Animals Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis Proteins - radiation effects Arabidopsis thaliana beta Catenin - genetics beta Catenin - metabolism beta Catenin - radiation effects Biochemistry Bioinformatics Biological Microscopy Biological Techniques Biomedical Engineering/Biotechnology Blotting, Western brief-communication Cell Culture Techniques Cell Nucleus - metabolism Cell Nucleus - radiation effects Cryptochromes - genetics Cryptochromes - metabolism Cryptochromes - radiation effects Cytoplasm - metabolism Cytoplasm - radiation effects Enzyme-Linked Immunosorbent Assay Fluorescence Recovery After Photobleaching Genetic aspects Genetic transcription HEK293 Cells Humans Life Sciences Light Light Signal Transduction Luminescent Proteins - metabolism Luminescent Proteins - radiation effects Mammals Mice NIH 3T3 Cells Photobiology Physiological aspects Plant genetics Protein biosynthesis Protein Multimerization - radiation effects Protein synthesis Proteomics Recombinant Fusion Proteins - metabolism Recombinant Fusion Proteins - radiation effects Red Fluorescent Protein rhoA GTP-Binding Protein - genetics rhoA GTP-Binding Protein - metabolism rhoA GTP-Binding Protein - radiation effects Transcription, Genetic Transfection Wnt Signaling Pathway - radiation effects Wnt3A Protein - genetics Wnt3A Protein - metabolism Wnt3A Protein - radiation effects
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