Tricellulin expression in brain endothelial and neural cells
Tricellulin is a tight junction (TJ) protein, which is not only concentrated at tricellular contacts but also present at bicellular contacts between epithelial tissues. We scrutinized the brain for tricellulin expression in endothelial and neural cells by using real-time polymerase chain reaction, W...
Gespeichert in:
| Veröffentlicht in: | Cell and tissue research 2013-03, Vol.351 (3), p.397-407 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 407 |
|---|---|
| container_issue | 3 |
| container_start_page | 397 |
| container_title | Cell and tissue research |
| container_volume | 351 |
| creator | Mariano, Cibelle Palmela, Inês Pereira, Pedro Fernandes, Adelaide Falcão, Ana Sofia Cardoso, Filipa Lourenço Vaz, Ana Rita Campos, Alexandre Rainha Gonçalves-Ferreira, António Kim, Kwang Sik Brites, Dora Brito, Maria Alexandra |
| description | Tricellulin is a tight junction (TJ) protein, which is not only concentrated at tricellular contacts but also present at bicellular contacts between epithelial tissues. We scrutinized the brain for tricellulin expression in endothelial and neural cells by using real-time polymerase chain reaction, Western blot and immunohistochemical and immunocytochemical analysis of cultured brain cells and paraffin sections of brain. Tricellulin mRNA was detected in primary cultures and in a cell line of human brain microvascular endothelial cells. Protein expression was confirmed by Western blot and immunofluorescence analysis, which further highlighted the localization of tricellulin in the cell membrane at tricellular and along bicellular contacts, and in the nucleus and perinuclear region. Compared with the well-studied TJ protein, zonula occludens-1, tricellulin expression was less marked at the cell membrane but more evident in the nuclear and perinuclear regions. The presence of tricellulin in cultured endothelial cells was corroborated by immunohistochemical and immunofluorescence staining in brain blood vessels, where it was colocalized with another TJ protein, claudin-5. Tricellulin mRNA was detected in neurons and astrocytes, whereas protein expression was observed in astrocytes but not in neurons, as shown by immunofluorescence analysis. This study reveals the presence and subcellular distribution of tricellulin in brain endothelial cells, both in vitro and in situ and its colocalization with other relevant TJ proteins. Moreover, it demonstrates the expression of the protein in astrocytes opening new avenues for future research to establish the biological significance of tricellulin expression in glial cells. |
| doi_str_mv | 10.1007/s00441-012-1529-y |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1313420930</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A335189033</galeid><sourcerecordid>A335189033</sourcerecordid><originalsourceid>FETCH-LOGICAL-c494t-8c7ae81dd3ef07c2a20fe4b16aac2eb4ed6cee1588757b71c9de6766b6f184823</originalsourceid><addsrcrecordid>eNp9klFrFDEQx4Mo9qx-AF_0QBBftmaS7CYLvpSiVij0wRZ8C9ns7F1KLjmTXfC-vVm2aluk5CHJ5PcfZvIfQl4DPQFK5cdMqRBQUWAV1KytDk_ICgRnFVVSPSUryimrZNP8OCIvcr6hFETTtM_JEeOsprVkK_LpKjmL3k_ehTX-2ifM2cWwLrcumTkW-jhu0Tvj1yb064BTKsdZk1-SZ4PxGV_d7sfk-svnq7Pz6uLy67ez04vKilaMlbLSoIK-5zhQaZlhdEDRQWOMZdgJ7BuLCLVSspadBNv22JSyu2YAJRTjx-TDknef4s8J86h3Ls8VmIBxyho4cMFoy2lB3z1Ab-KUQqluppisW6bgH7UxHrULQxyTsXNSfcp5DaqlnBfq5D9UWT3unI0BB1fi9wTv7wi2aPy4zdFPY_nRfB-EBbQp5pxw0PvkdiYdNFA9W6sXa3WxVs_W6kPRvLntbOp22P9V_PGyAGwBcnkKG0x3Wn8k69tFNJiozSa5rK-_szIoZVhKoaLlvwGA9LT9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1312759281</pqid></control><display><type>article</type><title>Tricellulin expression in brain endothelial and neural cells</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Mariano, Cibelle ; Palmela, Inês ; Pereira, Pedro ; Fernandes, Adelaide ; Falcão, Ana Sofia ; Cardoso, Filipa Lourenço ; Vaz, Ana Rita ; Campos, Alexandre Rainha ; Gonçalves-Ferreira, António ; Kim, Kwang Sik ; Brites, Dora ; Brito, Maria Alexandra</creator><creatorcontrib>Mariano, Cibelle ; Palmela, Inês ; Pereira, Pedro ; Fernandes, Adelaide ; Falcão, Ana Sofia ; Cardoso, Filipa Lourenço ; Vaz, Ana Rita ; Campos, Alexandre Rainha ; Gonçalves-Ferreira, António ; Kim, Kwang Sik ; Brites, Dora ; Brito, Maria Alexandra</creatorcontrib><description>Tricellulin is a tight junction (TJ) protein, which is not only concentrated at tricellular contacts but also present at bicellular contacts between epithelial tissues. We scrutinized the brain for tricellulin expression in endothelial and neural cells by using real-time polymerase chain reaction, Western blot and immunohistochemical and immunocytochemical analysis of cultured brain cells and paraffin sections of brain. Tricellulin mRNA was detected in primary cultures and in a cell line of human brain microvascular endothelial cells. Protein expression was confirmed by Western blot and immunofluorescence analysis, which further highlighted the localization of tricellulin in the cell membrane at tricellular and along bicellular contacts, and in the nucleus and perinuclear region. Compared with the well-studied TJ protein, zonula occludens-1, tricellulin expression was less marked at the cell membrane but more evident in the nuclear and perinuclear regions. The presence of tricellulin in cultured endothelial cells was corroborated by immunohistochemical and immunofluorescence staining in brain blood vessels, where it was colocalized with another TJ protein, claudin-5. Tricellulin mRNA was detected in neurons and astrocytes, whereas protein expression was observed in astrocytes but not in neurons, as shown by immunofluorescence analysis. This study reveals the presence and subcellular distribution of tricellulin in brain endothelial cells, both in vitro and in situ and its colocalization with other relevant TJ proteins. Moreover, it demonstrates the expression of the protein in astrocytes opening new avenues for future research to establish the biological significance of tricellulin expression in glial cells.</description><identifier>ISSN: 0302-766X</identifier><identifier>EISSN: 1432-0878</identifier><identifier>DOI: 10.1007/s00441-012-1529-y</identifier><identifier>PMID: 23250572</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Analysis ; Animals ; astrocytes ; Astrocytes - cytology ; Astrocytes - metabolism ; Biomedical and Life Sciences ; Biomedicine ; blood vessels ; Brain ; Brain - blood supply ; Brain - cytology ; cell culture ; cell membranes ; Cells, Cultured ; Cytochemistry ; endothelial cells ; Endothelial Cells - cytology ; Endothelial Cells - metabolism ; Endothelium ; fluorescent antibody technique ; Human Genetics ; Humans ; immunohistochemistry ; MARVEL Domain Containing 2 Protein - metabolism ; messenger RNA ; Microvessels - cytology ; Molecular Medicine ; neurons ; Neurons - cytology ; Neurons - metabolism ; protein synthesis ; Protein Transport ; proteins ; Proteomics ; quantitative polymerase chain reaction ; Rats ; Rats, Wistar ; RNA ; Short Communication ; tight junctions ; Toy industry ; Western blotting</subject><ispartof>Cell and tissue research, 2013-03, Vol.351 (3), p.397-407</ispartof><rights>Springer-Verlag Berlin Heidelberg 2012</rights><rights>COPYRIGHT 2013 Springer</rights><rights>Springer-Verlag 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-8c7ae81dd3ef07c2a20fe4b16aac2eb4ed6cee1588757b71c9de6766b6f184823</citedby><cites>FETCH-LOGICAL-c494t-8c7ae81dd3ef07c2a20fe4b16aac2eb4ed6cee1588757b71c9de6766b6f184823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00441-012-1529-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00441-012-1529-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23250572$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mariano, Cibelle</creatorcontrib><creatorcontrib>Palmela, Inês</creatorcontrib><creatorcontrib>Pereira, Pedro</creatorcontrib><creatorcontrib>Fernandes, Adelaide</creatorcontrib><creatorcontrib>Falcão, Ana Sofia</creatorcontrib><creatorcontrib>Cardoso, Filipa Lourenço</creatorcontrib><creatorcontrib>Vaz, Ana Rita</creatorcontrib><creatorcontrib>Campos, Alexandre Rainha</creatorcontrib><creatorcontrib>Gonçalves-Ferreira, António</creatorcontrib><creatorcontrib>Kim, Kwang Sik</creatorcontrib><creatorcontrib>Brites, Dora</creatorcontrib><creatorcontrib>Brito, Maria Alexandra</creatorcontrib><title>Tricellulin expression in brain endothelial and neural cells</title><title>Cell and tissue research</title><addtitle>Cell Tissue Res</addtitle><addtitle>Cell Tissue Res</addtitle><description>Tricellulin is a tight junction (TJ) protein, which is not only concentrated at tricellular contacts but also present at bicellular contacts between epithelial tissues. We scrutinized the brain for tricellulin expression in endothelial and neural cells by using real-time polymerase chain reaction, Western blot and immunohistochemical and immunocytochemical analysis of cultured brain cells and paraffin sections of brain. Tricellulin mRNA was detected in primary cultures and in a cell line of human brain microvascular endothelial cells. Protein expression was confirmed by Western blot and immunofluorescence analysis, which further highlighted the localization of tricellulin in the cell membrane at tricellular and along bicellular contacts, and in the nucleus and perinuclear region. Compared with the well-studied TJ protein, zonula occludens-1, tricellulin expression was less marked at the cell membrane but more evident in the nuclear and perinuclear regions. The presence of tricellulin in cultured endothelial cells was corroborated by immunohistochemical and immunofluorescence staining in brain blood vessels, where it was colocalized with another TJ protein, claudin-5. Tricellulin mRNA was detected in neurons and astrocytes, whereas protein expression was observed in astrocytes but not in neurons, as shown by immunofluorescence analysis. This study reveals the presence and subcellular distribution of tricellulin in brain endothelial cells, both in vitro and in situ and its colocalization with other relevant TJ proteins. Moreover, it demonstrates the expression of the protein in astrocytes opening new avenues for future research to establish the biological significance of tricellulin expression in glial cells.</description><subject>Analysis</subject><subject>Animals</subject><subject>astrocytes</subject><subject>Astrocytes - cytology</subject><subject>Astrocytes - metabolism</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>blood vessels</subject><subject>Brain</subject><subject>Brain - blood supply</subject><subject>Brain - cytology</subject><subject>cell culture</subject><subject>cell membranes</subject><subject>Cells, Cultured</subject><subject>Cytochemistry</subject><subject>endothelial cells</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - metabolism</subject><subject>Endothelium</subject><subject>fluorescent antibody technique</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>immunohistochemistry</subject><subject>MARVEL Domain Containing 2 Protein - metabolism</subject><subject>messenger RNA</subject><subject>Microvessels - cytology</subject><subject>Molecular Medicine</subject><subject>neurons</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>protein synthesis</subject><subject>Protein Transport</subject><subject>proteins</subject><subject>Proteomics</subject><subject>quantitative polymerase chain reaction</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA</subject><subject>Short Communication</subject><subject>tight junctions</subject><subject>Toy industry</subject><subject>Western blotting</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9klFrFDEQx4Mo9qx-AF_0QBBftmaS7CYLvpSiVij0wRZ8C9ns7F1KLjmTXfC-vVm2aluk5CHJ5PcfZvIfQl4DPQFK5cdMqRBQUWAV1KytDk_ICgRnFVVSPSUryimrZNP8OCIvcr6hFETTtM_JEeOsprVkK_LpKjmL3k_ehTX-2ifM2cWwLrcumTkW-jhu0Tvj1yb064BTKsdZk1-SZ4PxGV_d7sfk-svnq7Pz6uLy67ez04vKilaMlbLSoIK-5zhQaZlhdEDRQWOMZdgJ7BuLCLVSspadBNv22JSyu2YAJRTjx-TDknef4s8J86h3Ls8VmIBxyho4cMFoy2lB3z1Ab-KUQqluppisW6bgH7UxHrULQxyTsXNSfcp5DaqlnBfq5D9UWT3unI0BB1fi9wTv7wi2aPy4zdFPY_nRfB-EBbQp5pxw0PvkdiYdNFA9W6sXa3WxVs_W6kPRvLntbOp22P9V_PGyAGwBcnkKG0x3Wn8k69tFNJiozSa5rK-_szIoZVhKoaLlvwGA9LT9</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Mariano, Cibelle</creator><creator>Palmela, Inês</creator><creator>Pereira, Pedro</creator><creator>Fernandes, Adelaide</creator><creator>Falcão, Ana Sofia</creator><creator>Cardoso, Filipa Lourenço</creator><creator>Vaz, Ana Rita</creator><creator>Campos, Alexandre Rainha</creator><creator>Gonçalves-Ferreira, António</creator><creator>Kim, Kwang Sik</creator><creator>Brites, Dora</creator><creator>Brito, Maria Alexandra</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7RV</scope><scope>7SS</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20130301</creationdate><title>Tricellulin expression in brain endothelial and neural cells</title><author>Mariano, Cibelle ; Palmela, Inês ; Pereira, Pedro ; Fernandes, Adelaide ; Falcão, Ana Sofia ; Cardoso, Filipa Lourenço ; Vaz, Ana Rita ; Campos, Alexandre Rainha ; Gonçalves-Ferreira, António ; Kim, Kwang Sik ; Brites, Dora ; Brito, Maria Alexandra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-8c7ae81dd3ef07c2a20fe4b16aac2eb4ed6cee1588757b71c9de6766b6f184823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>astrocytes</topic><topic>Astrocytes - cytology</topic><topic>Astrocytes - metabolism</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>blood vessels</topic><topic>Brain</topic><topic>Brain - blood supply</topic><topic>Brain - cytology</topic><topic>cell culture</topic><topic>cell membranes</topic><topic>Cells, Cultured</topic><topic>Cytochemistry</topic><topic>endothelial cells</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - metabolism</topic><topic>Endothelium</topic><topic>fluorescent antibody technique</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>immunohistochemistry</topic><topic>MARVEL Domain Containing 2 Protein - metabolism</topic><topic>messenger RNA</topic><topic>Microvessels - cytology</topic><topic>Molecular Medicine</topic><topic>neurons</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>protein synthesis</topic><topic>Protein Transport</topic><topic>proteins</topic><topic>Proteomics</topic><topic>quantitative polymerase chain reaction</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA</topic><topic>Short Communication</topic><topic>tight junctions</topic><topic>Toy industry</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mariano, Cibelle</creatorcontrib><creatorcontrib>Palmela, Inês</creatorcontrib><creatorcontrib>Pereira, Pedro</creatorcontrib><creatorcontrib>Fernandes, Adelaide</creatorcontrib><creatorcontrib>Falcão, Ana Sofia</creatorcontrib><creatorcontrib>Cardoso, Filipa Lourenço</creatorcontrib><creatorcontrib>Vaz, Ana Rita</creatorcontrib><creatorcontrib>Campos, Alexandre Rainha</creatorcontrib><creatorcontrib>Gonçalves-Ferreira, António</creatorcontrib><creatorcontrib>Kim, Kwang Sik</creatorcontrib><creatorcontrib>Brites, Dora</creatorcontrib><creatorcontrib>Brito, Maria Alexandra</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mariano, Cibelle</au><au>Palmela, Inês</au><au>Pereira, Pedro</au><au>Fernandes, Adelaide</au><au>Falcão, Ana Sofia</au><au>Cardoso, Filipa Lourenço</au><au>Vaz, Ana Rita</au><au>Campos, Alexandre Rainha</au><au>Gonçalves-Ferreira, António</au><au>Kim, Kwang Sik</au><au>Brites, Dora</au><au>Brito, Maria Alexandra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tricellulin expression in brain endothelial and neural cells</atitle><jtitle>Cell and tissue research</jtitle><stitle>Cell Tissue Res</stitle><addtitle>Cell Tissue Res</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>351</volume><issue>3</issue><spage>397</spage><epage>407</epage><pages>397-407</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>Tricellulin is a tight junction (TJ) protein, which is not only concentrated at tricellular contacts but also present at bicellular contacts between epithelial tissues. We scrutinized the brain for tricellulin expression in endothelial and neural cells by using real-time polymerase chain reaction, Western blot and immunohistochemical and immunocytochemical analysis of cultured brain cells and paraffin sections of brain. Tricellulin mRNA was detected in primary cultures and in a cell line of human brain microvascular endothelial cells. Protein expression was confirmed by Western blot and immunofluorescence analysis, which further highlighted the localization of tricellulin in the cell membrane at tricellular and along bicellular contacts, and in the nucleus and perinuclear region. Compared with the well-studied TJ protein, zonula occludens-1, tricellulin expression was less marked at the cell membrane but more evident in the nuclear and perinuclear regions. The presence of tricellulin in cultured endothelial cells was corroborated by immunohistochemical and immunofluorescence staining in brain blood vessels, where it was colocalized with another TJ protein, claudin-5. Tricellulin mRNA was detected in neurons and astrocytes, whereas protein expression was observed in astrocytes but not in neurons, as shown by immunofluorescence analysis. This study reveals the presence and subcellular distribution of tricellulin in brain endothelial cells, both in vitro and in situ and its colocalization with other relevant TJ proteins. Moreover, it demonstrates the expression of the protein in astrocytes opening new avenues for future research to establish the biological significance of tricellulin expression in glial cells.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>23250572</pmid><doi>10.1007/s00441-012-1529-y</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0302-766X |
| ispartof | Cell and tissue research, 2013-03, Vol.351 (3), p.397-407 |
| issn | 0302-766X 1432-0878 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1313420930 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Analysis Animals astrocytes Astrocytes - cytology Astrocytes - metabolism Biomedical and Life Sciences Biomedicine blood vessels Brain Brain - blood supply Brain - cytology cell culture cell membranes Cells, Cultured Cytochemistry endothelial cells Endothelial Cells - cytology Endothelial Cells - metabolism Endothelium fluorescent antibody technique Human Genetics Humans immunohistochemistry MARVEL Domain Containing 2 Protein - metabolism messenger RNA Microvessels - cytology Molecular Medicine neurons Neurons - cytology Neurons - metabolism protein synthesis Protein Transport proteins Proteomics quantitative polymerase chain reaction Rats Rats, Wistar RNA Short Communication tight junctions Toy industry Western blotting |
| title | Tricellulin expression in brain endothelial and neural cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T04%3A40%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tricellulin%20expression%20in%20brain%20endothelial%20and%20neural%20cells&rft.jtitle=Cell%20and%20tissue%20research&rft.au=Mariano,%20Cibelle&rft.date=2013-03-01&rft.volume=351&rft.issue=3&rft.spage=397&rft.epage=407&rft.pages=397-407&rft.issn=0302-766X&rft.eissn=1432-0878&rft_id=info:doi/10.1007/s00441-012-1529-y&rft_dat=%3Cgale_proqu%3EA335189033%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1312759281&rft_id=info:pmid/23250572&rft_galeid=A335189033&rfr_iscdi=true |