Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells
Matrix metalloproteinases (MMPs) play an important role in cancer metastasis, cell migration and invasion. Herein, we investigated the effects of silibinin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell migration and MMP-9 expression in thyroid and breast cancer cells. Our results reveal...
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| Veröffentlicht in: | Oncology reports 2013-04, Vol.29 (4), p.1343-1348 |
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| creator | OH, SOO-JIN JUNG, SEUNG PIL HAN, JEONGHUN KIM, SANGMIN KIM, JEE SOO NAM, SEOK JIN LEE, JEONG EON KIM, JUNG-HAN |
| description | Matrix metalloproteinases (MMPs) play an important role in cancer metastasis, cell migration and invasion. Herein, we investigated the effects of silibinin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell migration and MMP-9 expression in thyroid and breast cancer cells. Our results revealed that the levels of MMP-9 mRNA and protein expression were significantly increased by TPA but not MMP-2 in TPC-1 and MCF7 cells. To verify the regulatory mechanism of TPA-induced MMP-9 expression, we treated TPC-1 and MCF7 cells with the MEK1/2 inhibitor, UO126, and TPA-induced MMP-9 expression was significantly decreased. We also found that TPA-induced cell migration and MMP-9 expression was significantly decreased by silibinin. In addition, TPA-induced phosphorylation of MEK and ERK was also inhibited by silibinin. Taken together, we suggest that silibinin suppresses TPA-induced cell migration and MMP-9 expression through the MEK/ERK-dependent pathway in thyroid and breast cancer cells. |
| doi_str_mv | 10.3892/or.2013.2252 |
| format | Article |
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Herein, we investigated the effects of silibinin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell migration and MMP-9 expression in thyroid and breast cancer cells. Our results revealed that the levels of MMP-9 mRNA and protein expression were significantly increased by TPA but not MMP-2 in TPC-1 and MCF7 cells. To verify the regulatory mechanism of TPA-induced MMP-9 expression, we treated TPC-1 and MCF7 cells with the MEK1/2 inhibitor, UO126, and TPA-induced MMP-9 expression was significantly decreased. We also found that TPA-induced cell migration and MMP-9 expression was significantly decreased by silibinin. In addition, TPA-induced phosphorylation of MEK and ERK was also inhibited by silibinin. Taken together, we suggest that silibinin suppresses TPA-induced cell migration and MMP-9 expression through the MEK/ERK-dependent pathway in thyroid and breast cancer cells.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or.2013.2252</identifier><identifier>PMID: 23353996</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Breast cancer ; Breast Neoplasms - drug therapy ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Butadienes - pharmacology ; Cell adhesion & migration ; Cell cycle ; Cell growth ; Cell Movement - drug effects ; Cell Movement - genetics ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic - drug effects ; Humans ; Kinases ; MAP Kinase Signaling System - drug effects ; MAP Kinase Signaling System - genetics ; Matrix Metalloproteinase 9 - genetics ; Matrix Metalloproteinase 9 - metabolism ; MCF-7 Cells ; Medical prognosis ; Metastasis ; MMP-9 ; Neoplasm Invasiveness - pathology ; Nitriles - pharmacology ; phorbol ester ; Phosphorylation ; silibinin ; Silymarin - administration & dosage ; Tetradecanoylphorbol Acetate - pharmacology ; Thyroid cancer ; Thyroid Neoplasms - drug therapy ; Thyroid Neoplasms - metabolism ; Thyroid Neoplasms - pathology ; Transcription factors</subject><ispartof>Oncology reports, 2013-04, Vol.29 (4), p.1343-1348</ispartof><rights>Copyright © 2013, Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-e8866a162d1f7c23388f35b63abbcc8c4c9a73210de32e8f0b6a026b9843ca5f3</citedby><cites>FETCH-LOGICAL-c388t-e8866a162d1f7c23388f35b63abbcc8c4c9a73210de32e8f0b6a026b9843ca5f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23353996$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>OH, SOO-JIN</creatorcontrib><creatorcontrib>JUNG, SEUNG PIL</creatorcontrib><creatorcontrib>HAN, JEONGHUN</creatorcontrib><creatorcontrib>KIM, SANGMIN</creatorcontrib><creatorcontrib>KIM, JEE SOO</creatorcontrib><creatorcontrib>NAM, SEOK JIN</creatorcontrib><creatorcontrib>LEE, JEONG EON</creatorcontrib><creatorcontrib>KIM, JUNG-HAN</creatorcontrib><title>Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>Matrix metalloproteinases (MMPs) play an important role in cancer metastasis, cell migration and invasion. Herein, we investigated the effects of silibinin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell migration and MMP-9 expression in thyroid and breast cancer cells. Our results revealed that the levels of MMP-9 mRNA and protein expression were significantly increased by TPA but not MMP-2 in TPC-1 and MCF7 cells. To verify the regulatory mechanism of TPA-induced MMP-9 expression, we treated TPC-1 and MCF7 cells with the MEK1/2 inhibitor, UO126, and TPA-induced MMP-9 expression was significantly decreased. We also found that TPA-induced cell migration and MMP-9 expression was significantly decreased by silibinin. In addition, TPA-induced phosphorylation of MEK and ERK was also inhibited by silibinin. Taken together, we suggest that silibinin suppresses TPA-induced cell migration and MMP-9 expression through the MEK/ERK-dependent pathway in thyroid and breast cancer cells.</description><subject>Breast cancer</subject><subject>Breast Neoplasms - drug therapy</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Butadienes - pharmacology</subject><subject>Cell adhesion & migration</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell Movement - drug effects</subject><subject>Cell Movement - genetics</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Humans</subject><subject>Kinases</subject><subject>MAP Kinase Signaling System - drug effects</subject><subject>MAP Kinase Signaling System - genetics</subject><subject>Matrix Metalloproteinase 9 - genetics</subject><subject>Matrix Metalloproteinase 9 - metabolism</subject><subject>MCF-7 Cells</subject><subject>Medical prognosis</subject><subject>Metastasis</subject><subject>MMP-9</subject><subject>Neoplasm Invasiveness - pathology</subject><subject>Nitriles - pharmacology</subject><subject>phorbol ester</subject><subject>Phosphorylation</subject><subject>silibinin</subject><subject>Silymarin - administration & dosage</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Thyroid cancer</subject><subject>Thyroid Neoplasms - drug therapy</subject><subject>Thyroid Neoplasms - metabolism</subject><subject>Thyroid Neoplasms - pathology</subject><subject>Transcription factors</subject><issn>1021-335X</issn><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkEtLAzEURoMovneuZUAQF6YmudNMshTxBYoFK7gLmUxGI21SkxnQf2_G1i5c5XLvycfHQeiIkhEIyS5CHDFCYcTYmG2gXVpJilkJdDPPhFEMMH7dQXspfRDCKsLlNtpheQlS8l1kn93M1c47Xzj_nqcuFdPJJXa-6Y1tCmNns2Lu3qLuXPCF9k3x-DjBsrBfi2hTGpb5b_f-HYNrfu91tDp1hdHe2PgbkA7QVqtnyR6u3n30cnM9vbrDD0-391eXD9iAEB22QnCuKWcNbSuTSwrRwrjmoOvaGGFKI3UFjJLGArOiJTXXhPFaihKMHrewj86WuYsYPnubOjV3aWigvQ19UhQoEyXlJcnoyT_0I_TR53aKSmC8ogSqTJ0vKRNDStG2ahHdXMdvRYka9KsQ1aBfDfozfrwK7eu5bdbwn-8MnC6BtMiqXBPSmgkRM4lJiSmUAD-w_4vr</recordid><startdate>20130401</startdate><enddate>20130401</enddate><creator>OH, SOO-JIN</creator><creator>JUNG, SEUNG PIL</creator><creator>HAN, JEONGHUN</creator><creator>KIM, SANGMIN</creator><creator>KIM, JEE SOO</creator><creator>NAM, SEOK JIN</creator><creator>LEE, JEONG EON</creator><creator>KIM, JUNG-HAN</creator><general>D.A. Spandidos</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20130401</creationdate><title>Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells</title><author>OH, SOO-JIN ; JUNG, SEUNG PIL ; HAN, JEONGHUN ; KIM, SANGMIN ; KIM, JEE SOO ; NAM, SEOK JIN ; LEE, JEONG EON ; KIM, JUNG-HAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-e8866a162d1f7c23388f35b63abbcc8c4c9a73210de32e8f0b6a026b9843ca5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Breast cancer</topic><topic>Breast Neoplasms - drug therapy</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Butadienes - pharmacology</topic><topic>Cell adhesion & migration</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell Movement - drug effects</topic><topic>Cell Movement - genetics</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Humans</topic><topic>Kinases</topic><topic>MAP Kinase Signaling System - drug effects</topic><topic>MAP Kinase Signaling System - genetics</topic><topic>Matrix Metalloproteinase 9 - genetics</topic><topic>Matrix Metalloproteinase 9 - metabolism</topic><topic>MCF-7 Cells</topic><topic>Medical prognosis</topic><topic>Metastasis</topic><topic>MMP-9</topic><topic>Neoplasm Invasiveness - pathology</topic><topic>Nitriles - pharmacology</topic><topic>phorbol ester</topic><topic>Phosphorylation</topic><topic>silibinin</topic><topic>Silymarin - administration & dosage</topic><topic>Tetradecanoylphorbol Acetate - 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Academic</collection><jtitle>Oncology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>OH, SOO-JIN</au><au>JUNG, SEUNG PIL</au><au>HAN, JEONGHUN</au><au>KIM, SANGMIN</au><au>KIM, JEE SOO</au><au>NAM, SEOK JIN</au><au>LEE, JEONG EON</au><au>KIM, JUNG-HAN</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells</atitle><jtitle>Oncology reports</jtitle><addtitle>Oncol Rep</addtitle><date>2013-04-01</date><risdate>2013</risdate><volume>29</volume><issue>4</issue><spage>1343</spage><epage>1348</epage><pages>1343-1348</pages><issn>1021-335X</issn><eissn>1791-2431</eissn><abstract>Matrix metalloproteinases (MMPs) play an important role in cancer metastasis, cell migration and invasion. Herein, we investigated the effects of silibinin on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell migration and MMP-9 expression in thyroid and breast cancer cells. Our results revealed that the levels of MMP-9 mRNA and protein expression were significantly increased by TPA but not MMP-2 in TPC-1 and MCF7 cells. To verify the regulatory mechanism of TPA-induced MMP-9 expression, we treated TPC-1 and MCF7 cells with the MEK1/2 inhibitor, UO126, and TPA-induced MMP-9 expression was significantly decreased. We also found that TPA-induced cell migration and MMP-9 expression was significantly decreased by silibinin. In addition, TPA-induced phosphorylation of MEK and ERK was also inhibited by silibinin. Taken together, we suggest that silibinin suppresses TPA-induced cell migration and MMP-9 expression through the MEK/ERK-dependent pathway in thyroid and breast cancer cells.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>23353996</pmid><doi>10.3892/or.2013.2252</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Oncology reports, 2013-04, Vol.29 (4), p.1343-1348 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Breast cancer Breast Neoplasms - drug therapy Breast Neoplasms - metabolism Breast Neoplasms - pathology Butadienes - pharmacology Cell adhesion & migration Cell cycle Cell growth Cell Movement - drug effects Cell Movement - genetics Female Gene expression Gene Expression Regulation, Neoplastic - drug effects Humans Kinases MAP Kinase Signaling System - drug effects MAP Kinase Signaling System - genetics Matrix Metalloproteinase 9 - genetics Matrix Metalloproteinase 9 - metabolism MCF-7 Cells Medical prognosis Metastasis MMP-9 Neoplasm Invasiveness - pathology Nitriles - pharmacology phorbol ester Phosphorylation silibinin Silymarin - administration & dosage Tetradecanoylphorbol Acetate - pharmacology Thyroid cancer Thyroid Neoplasms - drug therapy Thyroid Neoplasms - metabolism Thyroid Neoplasms - pathology Transcription factors |
| title | Silibinin inhibits TPA-induced cell migration and MMP-9 expression in thyroid and breast cancer cells |
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