Expression of Calcium/Calmodulin-Dependent Protein Kinase II and Pain-Related Behavior in Rat Models of Type 1 and Type 2 Diabetes
Abnormalities in peripheral nerves and dorsal root ganglia are noticed in the early stage of experimentally provoked diabetic neuropathy. Enzyme calcium/calmodulin-dependent protein kinase II (CaMKII) may have a modulating role in diabetic neuropathy because of its role in calcium homeostasis. A mod...
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| Veröffentlicht in: | Anesthesia and analgesia 2013-03, Vol.116 (3), p.712-721 |
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| container_title | Anesthesia and analgesia |
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| creator | Ferhatovic, Lejla Banozic, Adriana Kostic, Sandra Kurir, Tina Ticinovic Novak, Anela Vrdoljak, Luka Heffer, Marija Sapunar, Damir Puljak, Livia |
| description | Abnormalities in peripheral nerves and dorsal root ganglia are noticed in the early stage of experimentally provoked diabetic neuropathy. Enzyme calcium/calmodulin-dependent protein kinase II (CaMKII) may have a modulating role in diabetic neuropathy because of its role in calcium homeostasis.
A model of type 1 diabetes mellitus (DM1) was induced with 55 mg/kg of the streptozotocin and for DM2 induction a combination of high-fat diet and low-dose streptozotocin (35 mg/kg) was used. Pain-related behavior was analyzed using thermal and mechanical stimuli. Two weeks and 2 months after induction of diabetes rats were euthanized, and the expression of CaMKII and its isoforms in the dorsal root ganglia were analyzed using immunofluorescence.
Both types of diabetes were successfully induced, as confirmed by hyperglycemia. Increased pain-related behavior became evident in DM1 rats in 2 weeks after diabetes induction, but not in DM2 rats. The expression of total CaMKII and the phosphorylated α isoform of CaMKII increased in DM1 animals concurrently with pain-related behavior. Expression of α, β, γ, and δ isoforms in DM1 animals and expression of total CaMKII and all of its analyzed isoforms in DM2 animals remained unchanged.
Our findings may indicate involvement of CaMKII in transmission of nociceptive input early in DM1, but not in DM2. CaMKII may be a suitable pharmacological target for diabetic neuropathy. |
| doi_str_mv | 10.1213/ANE.0b013e318279b540 |
| format | Article |
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A model of type 1 diabetes mellitus (DM1) was induced with 55 mg/kg of the streptozotocin and for DM2 induction a combination of high-fat diet and low-dose streptozotocin (35 mg/kg) was used. Pain-related behavior was analyzed using thermal and mechanical stimuli. Two weeks and 2 months after induction of diabetes rats were euthanized, and the expression of CaMKII and its isoforms in the dorsal root ganglia were analyzed using immunofluorescence.
Both types of diabetes were successfully induced, as confirmed by hyperglycemia. Increased pain-related behavior became evident in DM1 rats in 2 weeks after diabetes induction, but not in DM2 rats. The expression of total CaMKII and the phosphorylated α isoform of CaMKII increased in DM1 animals concurrently with pain-related behavior. Expression of α, β, γ, and δ isoforms in DM1 animals and expression of total CaMKII and all of its analyzed isoforms in DM2 animals remained unchanged.
Our findings may indicate involvement of CaMKII in transmission of nociceptive input early in DM1, but not in DM2. CaMKII may be a suitable pharmacological target for diabetic neuropathy.</description><identifier>ISSN: 0003-2999</identifier><identifier>EISSN: 1526-7598</identifier><identifier>DOI: 10.1213/ANE.0b013e318279b540</identifier><identifier>PMID: 23400989</identifier><language>eng</language><publisher>United States: International Anesthesia Research Society</publisher><subject>Animals ; Behavior, Animal - physiology ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - biosynthesis ; Diabetes Mellitus, Type 1 - enzymology ; Diabetes Mellitus, Type 1 - physiopathology ; Diabetes Mellitus, Type 2 - enzymology ; Diabetes Mellitus, Type 2 - physiopathology ; Disease Models, Animal ; Gene Expression Regulation, Enzymologic ; Male ; Pain - enzymology ; Pain - physiopathology ; Pain Measurement - methods ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Anesthesia and analgesia, 2013-03, Vol.116 (3), p.712-721</ispartof><rights>International Anesthesia Research Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3980-d526671e89b87125d10a7705c8ec7a6840359f9212ca0590e9235736bcf754223</citedby><cites>FETCH-LOGICAL-c3980-d526671e89b87125d10a7705c8ec7a6840359f9212ca0590e9235736bcf754223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttp://ovidsp.ovid.com/ovidweb.cgi?T=JS&NEWS=n&CSC=Y&PAGE=fulltext&D=ovft&AN=00000539-201303000-00030$$EHTML$$P50$$Gwolterskluwer$$H</linktohtml><link.rule.ids>314,776,780,4595,27901,27902,65434</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23400989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferhatovic, Lejla</creatorcontrib><creatorcontrib>Banozic, Adriana</creatorcontrib><creatorcontrib>Kostic, Sandra</creatorcontrib><creatorcontrib>Kurir, Tina Ticinovic</creatorcontrib><creatorcontrib>Novak, Anela</creatorcontrib><creatorcontrib>Vrdoljak, Luka</creatorcontrib><creatorcontrib>Heffer, Marija</creatorcontrib><creatorcontrib>Sapunar, Damir</creatorcontrib><creatorcontrib>Puljak, Livia</creatorcontrib><title>Expression of Calcium/Calmodulin-Dependent Protein Kinase II and Pain-Related Behavior in Rat Models of Type 1 and Type 2 Diabetes</title><title>Anesthesia and analgesia</title><addtitle>Anesth Analg</addtitle><description>Abnormalities in peripheral nerves and dorsal root ganglia are noticed in the early stage of experimentally provoked diabetic neuropathy. Enzyme calcium/calmodulin-dependent protein kinase II (CaMKII) may have a modulating role in diabetic neuropathy because of its role in calcium homeostasis.
A model of type 1 diabetes mellitus (DM1) was induced with 55 mg/kg of the streptozotocin and for DM2 induction a combination of high-fat diet and low-dose streptozotocin (35 mg/kg) was used. Pain-related behavior was analyzed using thermal and mechanical stimuli. Two weeks and 2 months after induction of diabetes rats were euthanized, and the expression of CaMKII and its isoforms in the dorsal root ganglia were analyzed using immunofluorescence.
Both types of diabetes were successfully induced, as confirmed by hyperglycemia. Increased pain-related behavior became evident in DM1 rats in 2 weeks after diabetes induction, but not in DM2 rats. The expression of total CaMKII and the phosphorylated α isoform of CaMKII increased in DM1 animals concurrently with pain-related behavior. Expression of α, β, γ, and δ isoforms in DM1 animals and expression of total CaMKII and all of its analyzed isoforms in DM2 animals remained unchanged.
Our findings may indicate involvement of CaMKII in transmission of nociceptive input early in DM1, but not in DM2. CaMKII may be a suitable pharmacological target for diabetic neuropathy.</description><subject>Animals</subject><subject>Behavior, Animal - physiology</subject><subject>Calcium-Calmodulin-Dependent Protein Kinase Type 2 - biosynthesis</subject><subject>Diabetes Mellitus, Type 1 - enzymology</subject><subject>Diabetes Mellitus, Type 1 - physiopathology</subject><subject>Diabetes Mellitus, Type 2 - enzymology</subject><subject>Diabetes Mellitus, Type 2 - physiopathology</subject><subject>Disease Models, Animal</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Male</subject><subject>Pain - enzymology</subject><subject>Pain - physiopathology</subject><subject>Pain Measurement - methods</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>0003-2999</issn><issn>1526-7598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUcFu1DAQtRCILqV_gJCPXNKO7Ti2j2W7lBUFqqo9R04y0RqcONgJba98Od62gMRcZp703hvNG0LeMDhmnImT0y-bY2iACRRMc2UaWcIzsmKSV4WSRj8nKwAQBTfGHJBXKX3LkIGuXpIDLkoAo82K_NrcTRFTcmGkoadr61u3DCe5D6FbvBuLM5xw7HCc6WUMM7qRfnKjTUi3W2rHjl7aTLpCb2fs6Hvc2Z8uRJppV3amn0OHPu2dr-8npOxB8TByeuZsgzOm1-RFb33Co6d-SG4-bK7XH4uLr-fb9elF0QqjoejyYZViqE2jFeOyY2CVAtlqbJWtdAlCmt5wxlsL0gAaLqQSVdP2Spaci0Py7tF3iuHHgmmuB5da9N6OGJZUM8G4FhpMmanlI7WNIaWIfT1FN9h4XzOo9-nXOf36__Sz7O3ThqUZsPsr-hP3P9_b4GeM6btfbjHWO7R-3tWwLylMwbMviAyK_QdB_AZdUo4W</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Ferhatovic, Lejla</creator><creator>Banozic, Adriana</creator><creator>Kostic, Sandra</creator><creator>Kurir, Tina Ticinovic</creator><creator>Novak, Anela</creator><creator>Vrdoljak, Luka</creator><creator>Heffer, Marija</creator><creator>Sapunar, Damir</creator><creator>Puljak, Livia</creator><general>International Anesthesia Research Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130301</creationdate><title>Expression of Calcium/Calmodulin-Dependent Protein Kinase II and Pain-Related Behavior in Rat Models of Type 1 and Type 2 Diabetes</title><author>Ferhatovic, Lejla ; Banozic, Adriana ; Kostic, Sandra ; Kurir, Tina Ticinovic ; Novak, Anela ; Vrdoljak, Luka ; Heffer, Marija ; Sapunar, Damir ; Puljak, Livia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3980-d526671e89b87125d10a7705c8ec7a6840359f9212ca0590e9235736bcf754223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Behavior, Animal - physiology</topic><topic>Calcium-Calmodulin-Dependent Protein Kinase Type 2 - biosynthesis</topic><topic>Diabetes Mellitus, Type 1 - enzymology</topic><topic>Diabetes Mellitus, Type 1 - physiopathology</topic><topic>Diabetes Mellitus, Type 2 - enzymology</topic><topic>Diabetes Mellitus, Type 2 - physiopathology</topic><topic>Disease Models, Animal</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Male</topic><topic>Pain - enzymology</topic><topic>Pain - physiopathology</topic><topic>Pain Measurement - methods</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferhatovic, Lejla</creatorcontrib><creatorcontrib>Banozic, Adriana</creatorcontrib><creatorcontrib>Kostic, Sandra</creatorcontrib><creatorcontrib>Kurir, Tina Ticinovic</creatorcontrib><creatorcontrib>Novak, Anela</creatorcontrib><creatorcontrib>Vrdoljak, Luka</creatorcontrib><creatorcontrib>Heffer, Marija</creatorcontrib><creatorcontrib>Sapunar, Damir</creatorcontrib><creatorcontrib>Puljak, Livia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Anesthesia and analgesia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferhatovic, Lejla</au><au>Banozic, Adriana</au><au>Kostic, Sandra</au><au>Kurir, Tina Ticinovic</au><au>Novak, Anela</au><au>Vrdoljak, Luka</au><au>Heffer, Marija</au><au>Sapunar, Damir</au><au>Puljak, Livia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Calcium/Calmodulin-Dependent Protein Kinase II and Pain-Related Behavior in Rat Models of Type 1 and Type 2 Diabetes</atitle><jtitle>Anesthesia and analgesia</jtitle><addtitle>Anesth Analg</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>116</volume><issue>3</issue><spage>712</spage><epage>721</epage><pages>712-721</pages><issn>0003-2999</issn><eissn>1526-7598</eissn><abstract>Abnormalities in peripheral nerves and dorsal root ganglia are noticed in the early stage of experimentally provoked diabetic neuropathy. Enzyme calcium/calmodulin-dependent protein kinase II (CaMKII) may have a modulating role in diabetic neuropathy because of its role in calcium homeostasis.
A model of type 1 diabetes mellitus (DM1) was induced with 55 mg/kg of the streptozotocin and for DM2 induction a combination of high-fat diet and low-dose streptozotocin (35 mg/kg) was used. Pain-related behavior was analyzed using thermal and mechanical stimuli. Two weeks and 2 months after induction of diabetes rats were euthanized, and the expression of CaMKII and its isoforms in the dorsal root ganglia were analyzed using immunofluorescence.
Both types of diabetes were successfully induced, as confirmed by hyperglycemia. Increased pain-related behavior became evident in DM1 rats in 2 weeks after diabetes induction, but not in DM2 rats. The expression of total CaMKII and the phosphorylated α isoform of CaMKII increased in DM1 animals concurrently with pain-related behavior. Expression of α, β, γ, and δ isoforms in DM1 animals and expression of total CaMKII and all of its analyzed isoforms in DM2 animals remained unchanged.
Our findings may indicate involvement of CaMKII in transmission of nociceptive input early in DM1, but not in DM2. CaMKII may be a suitable pharmacological target for diabetic neuropathy.</abstract><cop>United States</cop><pub>International Anesthesia Research Society</pub><pmid>23400989</pmid><doi>10.1213/ANE.0b013e318279b540</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Behavior, Animal - physiology Calcium-Calmodulin-Dependent Protein Kinase Type 2 - biosynthesis Diabetes Mellitus, Type 1 - enzymology Diabetes Mellitus, Type 1 - physiopathology Diabetes Mellitus, Type 2 - enzymology Diabetes Mellitus, Type 2 - physiopathology Disease Models, Animal Gene Expression Regulation, Enzymologic Male Pain - enzymology Pain - physiopathology Pain Measurement - methods Rats Rats, Sprague-Dawley |
| title | Expression of Calcium/Calmodulin-Dependent Protein Kinase II and Pain-Related Behavior in Rat Models of Type 1 and Type 2 Diabetes |
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