Surgical implantation of an abdominal imaging window for intravital microscopy
High-resolution intravital microscopy through imaging windows has become an indispensable technique for the long-term visualization of dynamic processes in living animals. Easily accessible sites such as the skin, the breast and the skull can be imaged using various different imaging windows; howeve...
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| Veröffentlicht in: | Nature protocols 2013-03, Vol.8 (3), p.583-594 |
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| creator | Ritsma, Laila Steller, Ernst J A Ellenbroek, Saskia I J Kranenburg, Onno Borel Rinkes, Inne H M van Rheenen, Jacco |
| description | High-resolution intravital microscopy through imaging windows has become an indispensable technique for the long-term visualization of dynamic processes in living animals. Easily accessible sites such as the skin, the breast and the skull can be imaged using various different imaging windows; however, long-term imaging studies on cellular processes in abdominal organs are more challenging. These processes include colonization of the liver by metastatic tumor cells and the development of an immune response in the spleen. We have recently developed an abdominal imaging window (AIW) that allows long-term imaging of the liver, the pancreas, the intestine, the kidney and the spleen. Here we describe the detailed protocol for the optimal surgical implantation of the AIW, which takes ∼1 h, and subsequent multiphoton imaging, which takes up to 1 month. |
| doi_str_mv | 10.1038/nprot.2013.026 |
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Easily accessible sites such as the skin, the breast and the skull can be imaged using various different imaging windows; however, long-term imaging studies on cellular processes in abdominal organs are more challenging. These processes include colonization of the liver by metastatic tumor cells and the development of an immune response in the spleen. We have recently developed an abdominal imaging window (AIW) that allows long-term imaging of the liver, the pancreas, the intestine, the kidney and the spleen. Here we describe the detailed protocol for the optimal surgical implantation of the AIW, which takes ∼1 h, and subsequent multiphoton imaging, which takes up to 1 month.</description><identifier>ISSN: 1754-2189</identifier><identifier>EISSN: 1750-2799</identifier><identifier>DOI: 10.1038/nprot.2013.026</identifier><identifier>PMID: 23429719</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>14/69 ; 59 ; 631/1647/245 ; 631/1647/334 ; 64/60 ; Abdomen ; Abdomen - surgery ; Analytical Chemistry ; Animals ; Biocompatible Materials ; Biological Techniques ; Cell adhesion & migration ; Colonization ; Computational Biology/Bioinformatics ; Diagnostic imaging ; Diagnostic Imaging - methods ; Immune response ; Kidneys ; Life Sciences ; Liver ; Medical examination ; Metastasis ; Methods ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Inbred Strains ; Microarrays ; Microscope and microscopy ; Microscopy ; Organic Chemistry ; Physiology ; Protocol ; Spleen ; Titanium alloys ; Visualization</subject><ispartof>Nature protocols, 2013-03, Vol.8 (3), p.583-594</ispartof><rights>Springer Nature Limited 2013</rights><rights>COPYRIGHT 2013 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Mar 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-4b3b6a424b2930148f5556d70cecf05f7b8dba61aa05f6b753f71d4ed05311c23</citedby><cites>FETCH-LOGICAL-c530t-4b3b6a424b2930148f5556d70cecf05f7b8dba61aa05f6b753f71d4ed05311c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23429719$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ritsma, Laila</creatorcontrib><creatorcontrib>Steller, Ernst J A</creatorcontrib><creatorcontrib>Ellenbroek, Saskia I J</creatorcontrib><creatorcontrib>Kranenburg, Onno</creatorcontrib><creatorcontrib>Borel Rinkes, Inne H M</creatorcontrib><creatorcontrib>van Rheenen, Jacco</creatorcontrib><title>Surgical implantation of an abdominal imaging window for intravital microscopy</title><title>Nature protocols</title><addtitle>Nat Protoc</addtitle><addtitle>Nat Protoc</addtitle><description>High-resolution intravital microscopy through imaging windows has become an indispensable technique for the long-term visualization of dynamic processes in living animals. Easily accessible sites such as the skin, the breast and the skull can be imaged using various different imaging windows; however, long-term imaging studies on cellular processes in abdominal organs are more challenging. These processes include colonization of the liver by metastatic tumor cells and the development of an immune response in the spleen. We have recently developed an abdominal imaging window (AIW) that allows long-term imaging of the liver, the pancreas, the intestine, the kidney and the spleen. Here we describe the detailed protocol for the optimal surgical implantation of the AIW, which takes ∼1 h, and subsequent multiphoton imaging, which takes up to 1 month.</description><subject>14/69</subject><subject>59</subject><subject>631/1647/245</subject><subject>631/1647/334</subject><subject>64/60</subject><subject>Abdomen</subject><subject>Abdomen - surgery</subject><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Biocompatible Materials</subject><subject>Biological Techniques</subject><subject>Cell adhesion & migration</subject><subject>Colonization</subject><subject>Computational Biology/Bioinformatics</subject><subject>Diagnostic imaging</subject><subject>Diagnostic Imaging - methods</subject><subject>Immune response</subject><subject>Kidneys</subject><subject>Life Sciences</subject><subject>Liver</subject><subject>Medical examination</subject><subject>Metastasis</subject><subject>Methods</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred Strains</subject><subject>Microarrays</subject><subject>Microscope and microscopy</subject><subject>Microscopy</subject><subject>Organic Chemistry</subject><subject>Physiology</subject><subject>Protocol</subject><subject>Spleen</subject><subject>Titanium alloys</subject><subject>Visualization</subject><issn>1754-2189</issn><issn>1750-2799</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkc1v1DAQxS0EoqVw5YgicYFDtv6Mk2NVUahUFaktZ8tx7MhVYi-2Q-l_39ndgtpq5YM9nt886c1D6CPBK4JZexzWKZYVxYStMG1eoUMiBa6p7LrX2zevKWm7A_Qu51uMuWSNfIsOKOO0k6Q7RJfXSxq90VPl5_WkQ9HFx1BFV-lQ6X6Isw_bph59GKs7H4Z4V7mYKh9K0n98ge7sTYrZxPX9e_TG6SnbD4_3Efp19u3m9Ed98fP7-enJRW0Ew6XmPesbzSnvaccw4a0TQjSDxMYah4WTfTv0uiFaQ9H0UjAnycDtgAUjxFB2hL7sdMH-78XmomafjZ3AgY1LVoQRSiTjTQPo5xfobVwSmNpRvJPyKTXqySofXAR3ZiOqThgVvBNStECt9lBwBgs7iME6D__PBr4-GwCm2L9l1EvO6vz6aq_4Zpk5WafWCfae7hXBahO22oatNmErCBsGPj06W_rZDv_xf-kCcLwDMrTCaNMT6_slHwCAfrM2</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Ritsma, Laila</creator><creator>Steller, Ernst J A</creator><creator>Ellenbroek, Saskia I J</creator><creator>Kranenburg, Onno</creator><creator>Borel Rinkes, Inne H M</creator><creator>van Rheenen, Jacco</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20130301</creationdate><title>Surgical implantation of an abdominal imaging window for intravital microscopy</title><author>Ritsma, Laila ; Steller, Ernst J A ; Ellenbroek, Saskia I J ; Kranenburg, Onno ; Borel Rinkes, Inne H M ; van Rheenen, Jacco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-4b3b6a424b2930148f5556d70cecf05f7b8dba61aa05f6b753f71d4ed05311c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>14/69</topic><topic>59</topic><topic>631/1647/245</topic><topic>631/1647/334</topic><topic>64/60</topic><topic>Abdomen</topic><topic>Abdomen - surgery</topic><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Biocompatible Materials</topic><topic>Biological Techniques</topic><topic>Cell adhesion & migration</topic><topic>Colonization</topic><topic>Computational Biology/Bioinformatics</topic><topic>Diagnostic imaging</topic><topic>Diagnostic Imaging - methods</topic><topic>Immune response</topic><topic>Kidneys</topic><topic>Life Sciences</topic><topic>Liver</topic><topic>Medical examination</topic><topic>Metastasis</topic><topic>Methods</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred Strains</topic><topic>Microarrays</topic><topic>Microscope and microscopy</topic><topic>Microscopy</topic><topic>Organic Chemistry</topic><topic>Physiology</topic><topic>Protocol</topic><topic>Spleen</topic><topic>Titanium alloys</topic><topic>Visualization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ritsma, Laila</creatorcontrib><creatorcontrib>Steller, Ernst J A</creatorcontrib><creatorcontrib>Ellenbroek, Saskia I J</creatorcontrib><creatorcontrib>Kranenburg, Onno</creatorcontrib><creatorcontrib>Borel Rinkes, Inne H M</creatorcontrib><creatorcontrib>van Rheenen, Jacco</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature protocols</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ritsma, Laila</au><au>Steller, Ernst J A</au><au>Ellenbroek, Saskia I J</au><au>Kranenburg, Onno</au><au>Borel Rinkes, Inne H M</au><au>van Rheenen, Jacco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surgical implantation of an abdominal imaging window for intravital microscopy</atitle><jtitle>Nature protocols</jtitle><stitle>Nat Protoc</stitle><addtitle>Nat Protoc</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>8</volume><issue>3</issue><spage>583</spage><epage>594</epage><pages>583-594</pages><issn>1754-2189</issn><eissn>1750-2799</eissn><abstract>High-resolution intravital microscopy through imaging windows has become an indispensable technique for the long-term visualization of dynamic processes in living animals. Easily accessible sites such as the skin, the breast and the skull can be imaged using various different imaging windows; however, long-term imaging studies on cellular processes in abdominal organs are more challenging. These processes include colonization of the liver by metastatic tumor cells and the development of an immune response in the spleen. We have recently developed an abdominal imaging window (AIW) that allows long-term imaging of the liver, the pancreas, the intestine, the kidney and the spleen. Here we describe the detailed protocol for the optimal surgical implantation of the AIW, which takes ∼1 h, and subsequent multiphoton imaging, which takes up to 1 month.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>23429719</pmid><doi>10.1038/nprot.2013.026</doi><tpages>12</tpages></addata></record> |
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| subjects | 14/69 59 631/1647/245 631/1647/334 64/60 Abdomen Abdomen - surgery Analytical Chemistry Animals Biocompatible Materials Biological Techniques Cell adhesion & migration Colonization Computational Biology/Bioinformatics Diagnostic imaging Diagnostic Imaging - methods Immune response Kidneys Life Sciences Liver Medical examination Metastasis Methods Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred Strains Microarrays Microscope and microscopy Microscopy Organic Chemistry Physiology Protocol Spleen Titanium alloys Visualization |
| title | Surgical implantation of an abdominal imaging window for intravital microscopy |
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