Electrogenerated Chemiluminescence Immunosensor for Bacillus thuringiensis Cry1Ac Based on Fe3O4@Au Nanoparticles

A highly sensitive electrochemiluminescence (ECL) immunosensor for Cry1Ac was fabricated. The primary antibody anti-Cry1Ac was immobilized onto core–shell structural Fe3O4@Au nanoparticles. The antigen and glucose-oxidase-labeled secondary antibody were then successively combined to form sandwich-ty...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2013-02, Vol.61 (7), p.1435-1440
Hauptverfasser:	Li, Jianping, Xu, Qian, Wei, Xiaoping, Hao, Zaibin
Format:	Artikel
Sprache:	eng
Schlagworte:	antigen-antibody complex antigens Bacillus thuringiensis Bacillus thuringiensis - isolation & purification Bacterial Proteins - analysis Bacterial Proteins - chemistry Biological and medical sciences Biosensing Techniques - methods carbon chemiluminescence detection limit Electrodes Endotoxins - analysis Endotoxins - chemistry Ferric Compounds - chemistry Food industries Fundamental and applied biological sciences. Psychology glass glucose Glucose Oxidase - chemistry Hemolysin Proteins - analysis Hemolysin Proteins - chemistry hydrogen peroxide Hydrogen Peroxide - chemistry Limit of Detection Luminescence Luminol - chemistry Metal Nanoparticles - chemistry nanoparticles
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