Tell Me a Tale of TALEs
Pathogenic bacteria of the Xanthomonas and Ralstonia genus have developed resourceful strategies creating a favorable environment to multiply and colonize their host plants. One of these strategies involves the secretion and translocation of several families of effector proteins into the host cell....
Gespeichert in:
| Veröffentlicht in: | Molecular biotechnology 2013-02, Vol.53 (2), p.228-235 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 235 |
|---|---|
| container_issue | 2 |
| container_start_page | 228 |
| container_title | Molecular biotechnology |
| container_volume | 53 |
| creator | Muñoz Bodnar, Alejandra Bernal, Adriana Szurek, Boris López, Camilo E |
| description | Pathogenic bacteria of the Xanthomonas and Ralstonia genus have developed resourceful strategies creating a favorable environment to multiply and colonize their host plants. One of these strategies involves the secretion and translocation of several families of effector proteins into the host cell. The transcription activator-like effector (TALE) family forms a subset of proteins involved in the direct modulation of host gene expression. TALEs include a number of tandem 34-amino acid repeats in their central part, where specific residues variable in two adjacent positions determine DNA-binding in the host genome. The specificity of this binding and its predictable nature make TALEs a revolutionary tool for gene editing, functional analysis, modification of target gene expression, and directed mutagenesis. Several examples have been reported in higher organisms as diverse as plants, Drosophila, zebrafish, mouse, and even human cells. Here, we summarize the functions of TALEs in their natural context and the biotechnological perspectives of their use. |
| doi_str_mv | 10.1007/s12033-012-9619-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1291618048</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2858315621</sourcerecordid><originalsourceid>FETCH-LOGICAL-c429t-3d6a1dadb6b37a73a9a4fca3116cc587409ed6f983a8f135f6b7bb97fccd41d83</originalsourceid><addsrcrecordid>eNp9kD1PwzAQhi0EoqUwIxaIxMISuLNjOx6rqnxIRQyks-UkdtWqbYrdDPx7HKUgxMDkk_y8750eQq4Q7hFAPgSkwFgKSFMlUKXsiAyRc5UCA34cZ5AsFZDzATkLYQVAkWfslAwoQ8xymQ3JZWHX6-TVJiYpzNomjUuK8WwazsmJM-tgLw7viMwfp8XkOZ29Pb1MxrO0yqjap6wWBmtTl6Jk0khmlMlcZWK9qCoeN4CytXAqZyZ3yLgTpSxLJV1V1RnWORuRu75355uP1oa93ixDFW8yW9u0QSNVKDCHrENv_6CrpvXbeF2khBBUYrQxIthTlW9C8NbpnV9ujP_UCLqzpntrOlrTnTXdZa4PzW25sfVP4ltTBGgPhPi1XVj_a_U_rTd9yJlGm4VfBj1_p4AcAJRUOWVf6kB8fQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1266627103</pqid></control><display><type>article</type><title>Tell Me a Tale of TALEs</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Muñoz Bodnar, Alejandra ; Bernal, Adriana ; Szurek, Boris ; López, Camilo E</creator><creatorcontrib>Muñoz Bodnar, Alejandra ; Bernal, Adriana ; Szurek, Boris ; López, Camilo E</creatorcontrib><description>Pathogenic bacteria of the Xanthomonas and Ralstonia genus have developed resourceful strategies creating a favorable environment to multiply and colonize their host plants. One of these strategies involves the secretion and translocation of several families of effector proteins into the host cell. The transcription activator-like effector (TALE) family forms a subset of proteins involved in the direct modulation of host gene expression. TALEs include a number of tandem 34-amino acid repeats in their central part, where specific residues variable in two adjacent positions determine DNA-binding in the host genome. The specificity of this binding and its predictable nature make TALEs a revolutionary tool for gene editing, functional analysis, modification of target gene expression, and directed mutagenesis. Several examples have been reported in higher organisms as diverse as plants, Drosophila, zebrafish, mouse, and even human cells. Here, we summarize the functions of TALEs in their natural context and the biotechnological perspectives of their use.</description><identifier>ISSN: 1073-6085</identifier><identifier>EISSN: 1559-0305</identifier><identifier>DOI: 10.1007/s12033-012-9619-3</identifier><identifier>PMID: 23114874</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Amino acids ; Animals ; bacteria ; Bacterial Proteins - physiology ; Bacteriology ; Biochemistry ; Biological Techniques ; Biotechnology ; Cell Biology ; Chemistry ; Chemistry and Materials Science ; Danio rerio ; Drosophila ; Gene expression ; genes ; Genomes ; Genomics ; Homeodomain Proteins - physiology ; Host plants ; Host-Pathogen Interactions ; Human Genetics ; Humans ; mice ; Mutagenesis ; Plant pathology ; Protein Science ; Proteins ; Ralstonia ; Repressor Proteins - physiology ; Resource development ; Review ; Reviews ; Secretion ; Transcription Factors - physiology ; Translocation ; Xanthomonas</subject><ispartof>Molecular biotechnology, 2013-02, Vol.53 (2), p.228-235</ispartof><rights>Springer Science+Business Media New York 2012</rights><rights>Springer Science+Business Media New York 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-3d6a1dadb6b37a73a9a4fca3116cc587409ed6f983a8f135f6b7bb97fccd41d83</citedby><cites>FETCH-LOGICAL-c429t-3d6a1dadb6b37a73a9a4fca3116cc587409ed6f983a8f135f6b7bb97fccd41d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12033-012-9619-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12033-012-9619-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23114874$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Muñoz Bodnar, Alejandra</creatorcontrib><creatorcontrib>Bernal, Adriana</creatorcontrib><creatorcontrib>Szurek, Boris</creatorcontrib><creatorcontrib>López, Camilo E</creatorcontrib><title>Tell Me a Tale of TALEs</title><title>Molecular biotechnology</title><addtitle>Mol Biotechnol</addtitle><addtitle>Mol Biotechnol</addtitle><description>Pathogenic bacteria of the Xanthomonas and Ralstonia genus have developed resourceful strategies creating a favorable environment to multiply and colonize their host plants. One of these strategies involves the secretion and translocation of several families of effector proteins into the host cell. The transcription activator-like effector (TALE) family forms a subset of proteins involved in the direct modulation of host gene expression. TALEs include a number of tandem 34-amino acid repeats in their central part, where specific residues variable in two adjacent positions determine DNA-binding in the host genome. The specificity of this binding and its predictable nature make TALEs a revolutionary tool for gene editing, functional analysis, modification of target gene expression, and directed mutagenesis. Several examples have been reported in higher organisms as diverse as plants, Drosophila, zebrafish, mouse, and even human cells. Here, we summarize the functions of TALEs in their natural context and the biotechnological perspectives of their use.</description><subject>Amino acids</subject><subject>Animals</subject><subject>bacteria</subject><subject>Bacterial Proteins - physiology</subject><subject>Bacteriology</subject><subject>Biochemistry</subject><subject>Biological Techniques</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Danio rerio</subject><subject>Drosophila</subject><subject>Gene expression</subject><subject>genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Homeodomain Proteins - physiology</subject><subject>Host plants</subject><subject>Host-Pathogen Interactions</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>mice</subject><subject>Mutagenesis</subject><subject>Plant pathology</subject><subject>Protein Science</subject><subject>Proteins</subject><subject>Ralstonia</subject><subject>Repressor Proteins - physiology</subject><subject>Resource development</subject><subject>Review</subject><subject>Reviews</subject><subject>Secretion</subject><subject>Transcription Factors - physiology</subject><subject>Translocation</subject><subject>Xanthomonas</subject><issn>1073-6085</issn><issn>1559-0305</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kD1PwzAQhi0EoqUwIxaIxMISuLNjOx6rqnxIRQyks-UkdtWqbYrdDPx7HKUgxMDkk_y8750eQq4Q7hFAPgSkwFgKSFMlUKXsiAyRc5UCA34cZ5AsFZDzATkLYQVAkWfslAwoQ8xymQ3JZWHX6-TVJiYpzNomjUuK8WwazsmJM-tgLw7viMwfp8XkOZ29Pb1MxrO0yqjap6wWBmtTl6Jk0khmlMlcZWK9qCoeN4CytXAqZyZ3yLgTpSxLJV1V1RnWORuRu75355uP1oa93ixDFW8yW9u0QSNVKDCHrENv_6CrpvXbeF2khBBUYrQxIthTlW9C8NbpnV9ujP_UCLqzpntrOlrTnTXdZa4PzW25sfVP4ltTBGgPhPi1XVj_a_U_rTd9yJlGm4VfBj1_p4AcAJRUOWVf6kB8fQ</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Muñoz Bodnar, Alejandra</creator><creator>Bernal, Adriana</creator><creator>Szurek, Boris</creator><creator>López, Camilo E</creator><general>Springer-Verlag</general><general>Humana Press Inc</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope></search><sort><creationdate>20130201</creationdate><title>Tell Me a Tale of TALEs</title><author>Muñoz Bodnar, Alejandra ; Bernal, Adriana ; Szurek, Boris ; López, Camilo E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-3d6a1dadb6b37a73a9a4fca3116cc587409ed6f983a8f135f6b7bb97fccd41d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amino acids</topic><topic>Animals</topic><topic>bacteria</topic><topic>Bacterial Proteins - physiology</topic><topic>Bacteriology</topic><topic>Biochemistry</topic><topic>Biological Techniques</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Danio rerio</topic><topic>Drosophila</topic><topic>Gene expression</topic><topic>genes</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Homeodomain Proteins - physiology</topic><topic>Host plants</topic><topic>Host-Pathogen Interactions</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>mice</topic><topic>Mutagenesis</topic><topic>Plant pathology</topic><topic>Protein Science</topic><topic>Proteins</topic><topic>Ralstonia</topic><topic>Repressor Proteins - physiology</topic><topic>Resource development</topic><topic>Review</topic><topic>Reviews</topic><topic>Secretion</topic><topic>Transcription Factors - physiology</topic><topic>Translocation</topic><topic>Xanthomonas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muñoz Bodnar, Alejandra</creatorcontrib><creatorcontrib>Bernal, Adriana</creatorcontrib><creatorcontrib>Szurek, Boris</creatorcontrib><creatorcontrib>López, Camilo E</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><jtitle>Molecular biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muñoz Bodnar, Alejandra</au><au>Bernal, Adriana</au><au>Szurek, Boris</au><au>López, Camilo E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tell Me a Tale of TALEs</atitle><jtitle>Molecular biotechnology</jtitle><stitle>Mol Biotechnol</stitle><addtitle>Mol Biotechnol</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>53</volume><issue>2</issue><spage>228</spage><epage>235</epage><pages>228-235</pages><issn>1073-6085</issn><eissn>1559-0305</eissn><abstract>Pathogenic bacteria of the Xanthomonas and Ralstonia genus have developed resourceful strategies creating a favorable environment to multiply and colonize their host plants. One of these strategies involves the secretion and translocation of several families of effector proteins into the host cell. The transcription activator-like effector (TALE) family forms a subset of proteins involved in the direct modulation of host gene expression. TALEs include a number of tandem 34-amino acid repeats in their central part, where specific residues variable in two adjacent positions determine DNA-binding in the host genome. The specificity of this binding and its predictable nature make TALEs a revolutionary tool for gene editing, functional analysis, modification of target gene expression, and directed mutagenesis. Several examples have been reported in higher organisms as diverse as plants, Drosophila, zebrafish, mouse, and even human cells. Here, we summarize the functions of TALEs in their natural context and the biotechnological perspectives of their use.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>23114874</pmid><doi>10.1007/s12033-012-9619-3</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1073-6085 |
| ispartof | Molecular biotechnology, 2013-02, Vol.53 (2), p.228-235 |
| issn | 1073-6085 1559-0305 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1291618048 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Amino acids Animals bacteria Bacterial Proteins - physiology Bacteriology Biochemistry Biological Techniques Biotechnology Cell Biology Chemistry Chemistry and Materials Science Danio rerio Drosophila Gene expression genes Genomes Genomics Homeodomain Proteins - physiology Host plants Host-Pathogen Interactions Human Genetics Humans mice Mutagenesis Plant pathology Protein Science Proteins Ralstonia Repressor Proteins - physiology Resource development Review Reviews Secretion Transcription Factors - physiology Translocation Xanthomonas |
| title | Tell Me a Tale of TALEs |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T01%3A01%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tell%20Me%20a%20Tale%20of%20TALEs&rft.jtitle=Molecular%20biotechnology&rft.au=Mu%C3%B1oz%20Bodnar,%20Alejandra&rft.date=2013-02-01&rft.volume=53&rft.issue=2&rft.spage=228&rft.epage=235&rft.pages=228-235&rft.issn=1073-6085&rft.eissn=1559-0305&rft_id=info:doi/10.1007/s12033-012-9619-3&rft_dat=%3Cproquest_cross%3E2858315621%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1266627103&rft_id=info:pmid/23114874&rfr_iscdi=true |