Development of taxonomic rRNA-targeted probes of two harmful algae: Prorocentrum minimum and Kareniamikimotoi by fluorescence in situ hybridization

Harmful algal blooms recently have been under the spotlight throughout the world, because of their negative impact on the marine environment, aquaculture, fisheries as well as public health. The development of methods for rapid and precise identification and quantification of causative species is es...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Acta oceanologica Sinica 2013-02, Vol.32 (2), p.66-75
Hauptverfasser: Chen, Guofu, Wang, Quanfu, Zhang, Chunyun, Zhang, Baoyu, Wang, Guangce, Lu, Douding, Xu, Zhong, Yan, Peishen
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 75
container_issue 2
container_start_page 66
container_title Acta oceanologica Sinica
container_volume 32
creator Chen, Guofu
Wang, Quanfu
Zhang, Chunyun
Zhang, Baoyu
Wang, Guangce
Lu, Douding
Xu, Zhong
Yan, Peishen
description Harmful algal blooms recently have been under the spotlight throughout the world, because of their negative impact on the marine environment, aquaculture, fisheries as well as public health. The development of methods for rapid and precise identification and quantification of causative species is essential for the warning andmonitoring of blooms, among which the techniques based on taxonomic probes are themost favored. In this study, two harmful algae, i.e., Prorocentrum minimum and Karenia mikimotoi were taken into consideration. The partial large subunit rDNA (D1-D2) of both species were firstly PCR-amplified, cloned and sequenced. The obtained sequences were then introduced to carry out alignment analysis for gene specific regions. Three respective candidate probes for each species were designed and used to screen the optimal probe by performing fluorescence in situ hybridization (FISH) tests. The results showed that the probes Pmin0443 and Kmik0602 displayed the best hybridization for P. minimum and K. mikimotoi , respectively. Both the specific (taxonomic) (Pmin0443 and Kmik0602) and the control probes (UniC0512 and UniR0499) were used for cross-reactivity tests with other microalgae in our laboratory. The probes Pmin0443 and Kmik0602 are specific and could be served as taxonomic probes introduced into the techniques targeting rRNA, such as FISH, sandwich hybridization, and DNA-microarray assay of P. minimum and K. mikimotoi in the future. Finally, FISH analyses with both probes were performed on the simulated field samples. The probes could hybridize exclusively with the target cells well, and no significant difference ( p >0.05) was observed in the cell densities of the samples determined by FISH and light microscopy (LM). All suggest that the probes are specific and could be introduced into FISH for the monitoring of both harmful algae.
doi_str_mv 10.1007/s13131-013-0278-4
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1291617476</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2919601498</sourcerecordid><originalsourceid>FETCH-LOGICAL-c329t-4d556f1b370d704993333810aed276440c8696200b4cb63cfbce0050067528c93</originalsourceid><addsrcrecordid>eNp9kc1uFDEQhEeISCwJD8DNEhcuhvbfeMwtCuFHRAEhkLhZHo9n4zC2F9sDLK_BC-OwOSAk6D705atSl6rrHhJ4QgDk00JYWwyEYaBywPxOtyFDrzABpe52G6CCYQHi073ufinXAIIIJjfdz-fuq1vSLrhYUZpRNd9TTMFblN9fnuJq8tZVN6FdTqMrv4lvCV2ZHOZ1QWbZGvcMvcspJ9sc8hpQ8NGHdk2c0BuTXfQm-M8-pJo8GvdoXtaUXWm4dchHVHxd0dV-zH7yP0z1KZ50R7NZintwe4-7jy_OP5y9whdvX74-O73AllFVMZ-E6GcyMgmTBK4UazMQMG6isuccbMvfU4CR27Fndh6ta7kBeinoYBU77h4ffFu4L6srVQff_loWE11aiyZUkZ5ILvuGPvoLvU5rju073SDVA-Fq-B9F6MCFVIyLRpEDZXMqJbtZ77IPJu81AX1Tpj6UqVuZ-qZMzZuGHjSlsXHr8h_O_xT9AvrdooA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1284579345</pqid></control><display><type>article</type><title>Development of taxonomic rRNA-targeted probes of two harmful algae: Prorocentrum minimum and Kareniamikimotoi by fluorescence in situ hybridization</title><source>Springer Nature - Complete Springer Journals</source><source>ProQuest Central UK/Ireland</source><source>Alma/SFX Local Collection</source><source>ProQuest Central</source><creator>Chen, Guofu ; Wang, Quanfu ; Zhang, Chunyun ; Zhang, Baoyu ; Wang, Guangce ; Lu, Douding ; Xu, Zhong ; Yan, Peishen</creator><creatorcontrib>Chen, Guofu ; Wang, Quanfu ; Zhang, Chunyun ; Zhang, Baoyu ; Wang, Guangce ; Lu, Douding ; Xu, Zhong ; Yan, Peishen</creatorcontrib><description>Harmful algal blooms recently have been under the spotlight throughout the world, because of their negative impact on the marine environment, aquaculture, fisheries as well as public health. The development of methods for rapid and precise identification and quantification of causative species is essential for the warning andmonitoring of blooms, among which the techniques based on taxonomic probes are themost favored. In this study, two harmful algae, i.e., Prorocentrum minimum and Karenia mikimotoi were taken into consideration. The partial large subunit rDNA (D1-D2) of both species were firstly PCR-amplified, cloned and sequenced. The obtained sequences were then introduced to carry out alignment analysis for gene specific regions. Three respective candidate probes for each species were designed and used to screen the optimal probe by performing fluorescence in situ hybridization (FISH) tests. The results showed that the probes Pmin0443 and Kmik0602 displayed the best hybridization for P. minimum and K. mikimotoi , respectively. Both the specific (taxonomic) (Pmin0443 and Kmik0602) and the control probes (UniC0512 and UniR0499) were used for cross-reactivity tests with other microalgae in our laboratory. The probes Pmin0443 and Kmik0602 are specific and could be served as taxonomic probes introduced into the techniques targeting rRNA, such as FISH, sandwich hybridization, and DNA-microarray assay of P. minimum and K. mikimotoi in the future. Finally, FISH analyses with both probes were performed on the simulated field samples. The probes could hybridize exclusively with the target cells well, and no significant difference ( p &gt;0.05) was observed in the cell densities of the samples determined by FISH and light microscopy (LM). All suggest that the probes are specific and could be introduced into FISH for the monitoring of both harmful algae.</description><identifier>ISSN: 0253-505X</identifier><identifier>EISSN: 1869-1099</identifier><identifier>DOI: 10.1007/s13131-013-0278-4</identifier><language>eng</language><publisher>Heidelberg: The Chinese Society of Oceanography</publisher><subject>Algae ; Algal blooms ; Aquaculture ; Climatology ; Cross-reactivity ; DNA ; DNA microarrays ; DNA probes ; Earth and Environmental Science ; Earth Sciences ; Ecology ; Engineering Fluid Dynamics ; Environmental Chemistry ; Eutrophication ; Fish ; Fisheries ; Fluorescence ; Fluorescence in situ hybridization ; Hybridization ; Karenia mikimotoi ; Light microscopy ; Marine ; Marine &amp; Freshwater Sciences ; Marine environment ; Marine fish ; Microscopy ; Morphology ; Nucleotide sequence ; Oceanography ; Optical microscopy ; Phytoplankton ; Plankton ; Polyculture (aquaculture) ; Probes ; Prorocentrum minimum ; Public health ; rRNA ; Sensors ; Target cells ; Taxonomy ; Water quality</subject><ispartof>Acta oceanologica Sinica, 2013-02, Vol.32 (2), p.66-75</ispartof><rights>The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2013</rights><rights>The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg 2013.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c329t-4d556f1b370d704993333810aed276440c8696200b4cb63cfbce0050067528c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13131-013-0278-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1284579345?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,21368,27903,27904,33723,33724,41467,42536,43784,51298,64362,64364,64366,72216</link.rule.ids></links><search><creatorcontrib>Chen, Guofu</creatorcontrib><creatorcontrib>Wang, Quanfu</creatorcontrib><creatorcontrib>Zhang, Chunyun</creatorcontrib><creatorcontrib>Zhang, Baoyu</creatorcontrib><creatorcontrib>Wang, Guangce</creatorcontrib><creatorcontrib>Lu, Douding</creatorcontrib><creatorcontrib>Xu, Zhong</creatorcontrib><creatorcontrib>Yan, Peishen</creatorcontrib><title>Development of taxonomic rRNA-targeted probes of two harmful algae: Prorocentrum minimum and Kareniamikimotoi by fluorescence in situ hybridization</title><title>Acta oceanologica Sinica</title><addtitle>Acta Oceanol. Sin</addtitle><description>Harmful algal blooms recently have been under the spotlight throughout the world, because of their negative impact on the marine environment, aquaculture, fisheries as well as public health. The development of methods for rapid and precise identification and quantification of causative species is essential for the warning andmonitoring of blooms, among which the techniques based on taxonomic probes are themost favored. In this study, two harmful algae, i.e., Prorocentrum minimum and Karenia mikimotoi were taken into consideration. The partial large subunit rDNA (D1-D2) of both species were firstly PCR-amplified, cloned and sequenced. The obtained sequences were then introduced to carry out alignment analysis for gene specific regions. Three respective candidate probes for each species were designed and used to screen the optimal probe by performing fluorescence in situ hybridization (FISH) tests. The results showed that the probes Pmin0443 and Kmik0602 displayed the best hybridization for P. minimum and K. mikimotoi , respectively. Both the specific (taxonomic) (Pmin0443 and Kmik0602) and the control probes (UniC0512 and UniR0499) were used for cross-reactivity tests with other microalgae in our laboratory. The probes Pmin0443 and Kmik0602 are specific and could be served as taxonomic probes introduced into the techniques targeting rRNA, such as FISH, sandwich hybridization, and DNA-microarray assay of P. minimum and K. mikimotoi in the future. Finally, FISH analyses with both probes were performed on the simulated field samples. The probes could hybridize exclusively with the target cells well, and no significant difference ( p &gt;0.05) was observed in the cell densities of the samples determined by FISH and light microscopy (LM). All suggest that the probes are specific and could be introduced into FISH for the monitoring of both harmful algae.</description><subject>Algae</subject><subject>Algal blooms</subject><subject>Aquaculture</subject><subject>Climatology</subject><subject>Cross-reactivity</subject><subject>DNA</subject><subject>DNA microarrays</subject><subject>DNA probes</subject><subject>Earth and Environmental Science</subject><subject>Earth Sciences</subject><subject>Ecology</subject><subject>Engineering Fluid Dynamics</subject><subject>Environmental Chemistry</subject><subject>Eutrophication</subject><subject>Fish</subject><subject>Fisheries</subject><subject>Fluorescence</subject><subject>Fluorescence in situ hybridization</subject><subject>Hybridization</subject><subject>Karenia mikimotoi</subject><subject>Light microscopy</subject><subject>Marine</subject><subject>Marine &amp; Freshwater Sciences</subject><subject>Marine environment</subject><subject>Marine fish</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>Nucleotide sequence</subject><subject>Oceanography</subject><subject>Optical microscopy</subject><subject>Phytoplankton</subject><subject>Plankton</subject><subject>Polyculture (aquaculture)</subject><subject>Probes</subject><subject>Prorocentrum minimum</subject><subject>Public health</subject><subject>rRNA</subject><subject>Sensors</subject><subject>Target cells</subject><subject>Taxonomy</subject><subject>Water quality</subject><issn>0253-505X</issn><issn>1869-1099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1uFDEQhEeISCwJD8DNEhcuhvbfeMwtCuFHRAEhkLhZHo9n4zC2F9sDLK_BC-OwOSAk6D705atSl6rrHhJ4QgDk00JYWwyEYaBywPxOtyFDrzABpe52G6CCYQHi073ufinXAIIIJjfdz-fuq1vSLrhYUZpRNd9TTMFblN9fnuJq8tZVN6FdTqMrv4lvCV2ZHOZ1QWbZGvcMvcspJ9sc8hpQ8NGHdk2c0BuTXfQm-M8-pJo8GvdoXtaUXWm4dchHVHxd0dV-zH7yP0z1KZ50R7NZintwe4-7jy_OP5y9whdvX74-O73AllFVMZ-E6GcyMgmTBK4UazMQMG6isuccbMvfU4CR27Fndh6ta7kBeinoYBU77h4ffFu4L6srVQff_loWE11aiyZUkZ5ILvuGPvoLvU5rju073SDVA-Fq-B9F6MCFVIyLRpEDZXMqJbtZ77IPJu81AX1Tpj6UqVuZ-qZMzZuGHjSlsXHr8h_O_xT9AvrdooA</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Chen, Guofu</creator><creator>Wang, Quanfu</creator><creator>Zhang, Chunyun</creator><creator>Zhang, Baoyu</creator><creator>Wang, Guangce</creator><creator>Lu, Douding</creator><creator>Xu, Zhong</creator><creator>Yan, Peishen</creator><general>The Chinese Society of Oceanography</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7TG</scope><scope>7TN</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>GNUQQ</scope><scope>H96</scope><scope>HCIFZ</scope><scope>KL.</scope><scope>L.G</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>SOI</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>BBNVY</scope><scope>FR3</scope><scope>H95</scope><scope>H97</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>7TM</scope><scope>M7N</scope></search><sort><creationdate>20130201</creationdate><title>Development of taxonomic rRNA-targeted probes of two harmful algae: Prorocentrum minimum and Kareniamikimotoi by fluorescence in situ hybridization</title><author>Chen, Guofu ; Wang, Quanfu ; Zhang, Chunyun ; Zhang, Baoyu ; Wang, Guangce ; Lu, Douding ; Xu, Zhong ; Yan, Peishen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c329t-4d556f1b370d704993333810aed276440c8696200b4cb63cfbce0050067528c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Algae</topic><topic>Algal blooms</topic><topic>Aquaculture</topic><topic>Climatology</topic><topic>Cross-reactivity</topic><topic>DNA</topic><topic>DNA microarrays</topic><topic>DNA probes</topic><topic>Earth and Environmental Science</topic><topic>Earth Sciences</topic><topic>Ecology</topic><topic>Engineering Fluid Dynamics</topic><topic>Environmental Chemistry</topic><topic>Eutrophication</topic><topic>Fish</topic><topic>Fisheries</topic><topic>Fluorescence</topic><topic>Fluorescence in situ hybridization</topic><topic>Hybridization</topic><topic>Karenia mikimotoi</topic><topic>Light microscopy</topic><topic>Marine</topic><topic>Marine &amp; Freshwater Sciences</topic><topic>Marine environment</topic><topic>Marine fish</topic><topic>Microscopy</topic><topic>Morphology</topic><topic>Nucleotide sequence</topic><topic>Oceanography</topic><topic>Optical microscopy</topic><topic>Phytoplankton</topic><topic>Plankton</topic><topic>Polyculture (aquaculture)</topic><topic>Probes</topic><topic>Prorocentrum minimum</topic><topic>Public health</topic><topic>rRNA</topic><topic>Sensors</topic><topic>Target cells</topic><topic>Taxonomy</topic><topic>Water quality</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Guofu</creatorcontrib><creatorcontrib>Wang, Quanfu</creatorcontrib><creatorcontrib>Zhang, Chunyun</creatorcontrib><creatorcontrib>Zhang, Baoyu</creatorcontrib><creatorcontrib>Wang, Guangce</creatorcontrib><creatorcontrib>Lu, Douding</creatorcontrib><creatorcontrib>Xu, Zhong</creatorcontrib><creatorcontrib>Yan, Peishen</creatorcontrib><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Oceanic Abstracts</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric &amp; Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy &amp; Non-Living Resources</collection><collection>SciTech Premium Collection</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric &amp; Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Biological Science Collection</collection><collection>Engineering Research Database</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 3: Aquatic Pollution &amp; Environmental Quality</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Acta oceanologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Guofu</au><au>Wang, Quanfu</au><au>Zhang, Chunyun</au><au>Zhang, Baoyu</au><au>Wang, Guangce</au><au>Lu, Douding</au><au>Xu, Zhong</au><au>Yan, Peishen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of taxonomic rRNA-targeted probes of two harmful algae: Prorocentrum minimum and Kareniamikimotoi by fluorescence in situ hybridization</atitle><jtitle>Acta oceanologica Sinica</jtitle><stitle>Acta Oceanol. Sin</stitle><date>2013-02-01</date><risdate>2013</risdate><volume>32</volume><issue>2</issue><spage>66</spage><epage>75</epage><pages>66-75</pages><issn>0253-505X</issn><eissn>1869-1099</eissn><abstract>Harmful algal blooms recently have been under the spotlight throughout the world, because of their negative impact on the marine environment, aquaculture, fisheries as well as public health. The development of methods for rapid and precise identification and quantification of causative species is essential for the warning andmonitoring of blooms, among which the techniques based on taxonomic probes are themost favored. In this study, two harmful algae, i.e., Prorocentrum minimum and Karenia mikimotoi were taken into consideration. The partial large subunit rDNA (D1-D2) of both species were firstly PCR-amplified, cloned and sequenced. The obtained sequences were then introduced to carry out alignment analysis for gene specific regions. Three respective candidate probes for each species were designed and used to screen the optimal probe by performing fluorescence in situ hybridization (FISH) tests. The results showed that the probes Pmin0443 and Kmik0602 displayed the best hybridization for P. minimum and K. mikimotoi , respectively. Both the specific (taxonomic) (Pmin0443 and Kmik0602) and the control probes (UniC0512 and UniR0499) were used for cross-reactivity tests with other microalgae in our laboratory. The probes Pmin0443 and Kmik0602 are specific and could be served as taxonomic probes introduced into the techniques targeting rRNA, such as FISH, sandwich hybridization, and DNA-microarray assay of P. minimum and K. mikimotoi in the future. Finally, FISH analyses with both probes were performed on the simulated field samples. The probes could hybridize exclusively with the target cells well, and no significant difference ( p &gt;0.05) was observed in the cell densities of the samples determined by FISH and light microscopy (LM). All suggest that the probes are specific and could be introduced into FISH for the monitoring of both harmful algae.</abstract><cop>Heidelberg</cop><pub>The Chinese Society of Oceanography</pub><doi>10.1007/s13131-013-0278-4</doi><tpages>10</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0253-505X
ispartof Acta oceanologica Sinica, 2013-02, Vol.32 (2), p.66-75
issn 0253-505X
1869-1099
language eng
recordid cdi_proquest_miscellaneous_1291617476
source Springer Nature - Complete Springer Journals; ProQuest Central UK/Ireland; Alma/SFX Local Collection; ProQuest Central
subjects Algae
Algal blooms
Aquaculture
Climatology
Cross-reactivity
DNA
DNA microarrays
DNA probes
Earth and Environmental Science
Earth Sciences
Ecology
Engineering Fluid Dynamics
Environmental Chemistry
Eutrophication
Fish
Fisheries
Fluorescence
Fluorescence in situ hybridization
Hybridization
Karenia mikimotoi
Light microscopy
Marine
Marine & Freshwater Sciences
Marine environment
Marine fish
Microscopy
Morphology
Nucleotide sequence
Oceanography
Optical microscopy
Phytoplankton
Plankton
Polyculture (aquaculture)
Probes
Prorocentrum minimum
Public health
rRNA
Sensors
Target cells
Taxonomy
Water quality
title Development of taxonomic rRNA-targeted probes of two harmful algae: Prorocentrum minimum and Kareniamikimotoi by fluorescence in situ hybridization
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T16%3A16%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20taxonomic%20rRNA-targeted%20probes%20of%20two%20harmful%20algae:%20Prorocentrum%20minimum%20and%20Kareniamikimotoi%20by%20fluorescence%20in%20situ%20hybridization&rft.jtitle=Acta%20oceanologica%20Sinica&rft.au=Chen,%20Guofu&rft.date=2013-02-01&rft.volume=32&rft.issue=2&rft.spage=66&rft.epage=75&rft.pages=66-75&rft.issn=0253-505X&rft.eissn=1869-1099&rft_id=info:doi/10.1007/s13131-013-0278-4&rft_dat=%3Cproquest_cross%3E2919601498%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1284579345&rft_id=info:pmid/&rfr_iscdi=true