Effect of inter- and intragenic epistasis on the heritability of oil content in rapeseed (Brassica napus L.)
The loci detected by association mapping which are involved in the expression of important agronomic traits in crops often explain only a small proportion of the total genotypic variance. Here, 17 SNPs derived from 9 candidate genes from the triacylglycerol biosynthetic pathway were studied in an as...
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| Veröffentlicht in: | Theoretical and applied genetics 2013-02, Vol.126 (2), p.435-441 |
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| description | The loci detected by association mapping which are involved in the expression of important agronomic traits in crops often explain only a small proportion of the total genotypic variance. Here, 17 SNPs derived from 9 candidate genes from the triacylglycerol biosynthetic pathway were studied in an association analysis in a population of 685 diverse elite rapeseed inbred lines. The 685 lines were evaluated for oil content, as well as for glucosinolates, yield, and thousand-kernel weight in field trials at 4 locations. We detected main effects for most of the studied genes illustrating that genetic diversity for oil content can be exploited by the selection of favorable alleles. In addition to main effects, both intergenic and intragenic epistasis was detected that contributes to a considerable amount to the genotypic variance observed for oil content. The proportion of explained genotypic variance was doubled when in addition to main effects epistasis was considered. Therefore, a knowledge-based improvement of oil content in rapeseed should also take such favorable epistatic interactions into account. Our results suggest, that the observed high contribution of epistasis may to some extent explain the missing heritability in genome-wide association studies. |
| doi_str_mv | 10.1007/s00122-012-1991-7 |
| format | Article |
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Here, 17 SNPs derived from 9 candidate genes from the triacylglycerol biosynthetic pathway were studied in an association analysis in a population of 685 diverse elite rapeseed inbred lines. The 685 lines were evaluated for oil content, as well as for glucosinolates, yield, and thousand-kernel weight in field trials at 4 locations. We detected main effects for most of the studied genes illustrating that genetic diversity for oil content can be exploited by the selection of favorable alleles. In addition to main effects, both intergenic and intragenic epistasis was detected that contributes to a considerable amount to the genotypic variance observed for oil content. The proportion of explained genotypic variance was doubled when in addition to main effects epistasis was considered. Therefore, a knowledge-based improvement of oil content in rapeseed should also take such favorable epistatic interactions into account. 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Here, 17 SNPs derived from 9 candidate genes from the triacylglycerol biosynthetic pathway were studied in an association analysis in a population of 685 diverse elite rapeseed inbred lines. The 685 lines were evaluated for oil content, as well as for glucosinolates, yield, and thousand-kernel weight in field trials at 4 locations. We detected main effects for most of the studied genes illustrating that genetic diversity for oil content can be exploited by the selection of favorable alleles. In addition to main effects, both intergenic and intragenic epistasis was detected that contributes to a considerable amount to the genotypic variance observed for oil content. The proportion of explained genotypic variance was doubled when in addition to main effects epistasis was considered. Therefore, a knowledge-based improvement of oil content in rapeseed should also take such favorable epistatic interactions into account. 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| subjects | Agriculture agronomic traits alleles Biochemistry Biomedical and Life Sciences Biosynthesis Biotechnology Brassica napus Brassica rapa - genetics Brassica rapa - growth & development Chromosome Mapping crops Epigenetic inheritance Epigenetics epistasis Epistasis, Genetic - genetics Fatty acids field experimentation Genes, Plant - genetics Genetic aspects Genetic epistasis Genetic Markers genetic variation Genomes glucosinolates heritability inbred lines Kinases Life Sciences lipid content loci Metabolism Oils - metabolism Original Paper Physiological aspects Plant Biochemistry Plant Breeding/Biotechnology Plant Genetics and Genomics Quantitative Trait, Heritable Rape plants Rapeseed Seeds - chemistry Seeds - genetics single nucleotide polymorphism triacylglycerols variance |
| title | Effect of inter- and intragenic epistasis on the heritability of oil content in rapeseed (Brassica napus L.) |
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