Localizing coordinates of cerebral ischemic tissue without the need of staining in a rat model of focal cerebral infarct
Biochemical and metabolic analysis of ischemic cerebral tissue is central in stroke investigation and is usually performed in animal stroke models, such as the permanent occlusion of the middle cerebral artery (MCAO) in the rat that we have used. To be sure that the sample is from infarct tissue, it...
Gespeichert in:
| Veröffentlicht in: | Metabolic brain disease 2013-03, Vol.28 (1), p.21-24 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 24 |
|---|---|
| container_issue | 1 |
| container_start_page | 21 |
| container_title | Metabolic brain disease |
| container_volume | 28 |
| creator | Ruiz-Crespo, Silvia Trejo-Gabriel-Galán, Jose M. Coma-del-Corral, Maria Jesus |
| description | Biochemical and metabolic analysis of ischemic cerebral tissue is central in stroke investigation and is usually performed in animal stroke models, such as the permanent occlusion of the middle cerebral artery (MCAO) in the rat that we have used. To be sure that the sample is from infarct tissue, it is differentiated from the surrounding normal tissue by staining, usually with 2,3,5-triphenyltetrazolium chloride (TTC), but staining can hamper biochemical colorimetric analysis. We performed this study to avoid this obstacle. A cerebral infarct was provoked in a sample of 10 rats and the brain was cut in coronal sections that were stained with TTC so that the unstained, infarct areas could be delineated in a template of each section in which areas with infarct in all animals were delineated. We calculated infarct coordinates and depth so that the infarct tissue can be sampled without staining. For more precision, the ischemic cortex can be delimited staining its surface before sectioning and cortical tissue into which TTC diffuses can be afterwards discarded, as we had previously measured the TTC diffusion depth in rat brains. |
| doi_str_mv | 10.1007/s11011-012-9359-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1291609899</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2881433321</sourcerecordid><originalsourceid>FETCH-LOGICAL-c405t-57ef51864fcb0633a834e7a466a65125e152fc8abeee80b7d90a57c8100313f73</originalsourceid><addsrcrecordid>eNqNkc9rFTEQx4NY7LP6B3iRgBcvq5nNZjc5SvFH4YEXew7Z7KQvZTepSRaf_evN8qoUQfA0h_nMZ5j5EvIK2DtgbHifARhAw6BtFBeqOT4hOxADbwbei6dkx6QUzdApdk6e53zLGOMC1DNy3nLomeRiR477aM3s7324oTbGNPlgCmYaHbWYcExmpj7bAy7e0uJzXpH-8OUQ10LLAWlAnDY4F-PDJvGBGppMoUuccN5abtvwyBacSba8IGfOzBlfPtQLcv3p47fLL83-6-eryw_7xnZMlEYM6ATIvnN2ZD3nRvIOB9P1vekFtAJBtM5KMyKiZOMwKWbEYGX9DwfuBn5B3p68dyl-XzEXvdR7cJ5NwLhmDa2qv1BSqf9AZVdprnhF3_yF3sY1hXrIRnHRQsugUnCibIo5J3T6LvnFpJ8amN4S1KcEdU1QbwnqY515_WBexwWnPxO_I6tAewJybYUbTI9W_9P6C_Iqpl0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1283521201</pqid></control><display><type>article</type><title>Localizing coordinates of cerebral ischemic tissue without the need of staining in a rat model of focal cerebral infarct</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Ruiz-Crespo, Silvia ; Trejo-Gabriel-Galán, Jose M. ; Coma-del-Corral, Maria Jesus</creator><creatorcontrib>Ruiz-Crespo, Silvia ; Trejo-Gabriel-Galán, Jose M. ; Coma-del-Corral, Maria Jesus</creatorcontrib><description>Biochemical and metabolic analysis of ischemic cerebral tissue is central in stroke investigation and is usually performed in animal stroke models, such as the permanent occlusion of the middle cerebral artery (MCAO) in the rat that we have used. To be sure that the sample is from infarct tissue, it is differentiated from the surrounding normal tissue by staining, usually with 2,3,5-triphenyltetrazolium chloride (TTC), but staining can hamper biochemical colorimetric analysis. We performed this study to avoid this obstacle. A cerebral infarct was provoked in a sample of 10 rats and the brain was cut in coronal sections that were stained with TTC so that the unstained, infarct areas could be delineated in a template of each section in which areas with infarct in all animals were delineated. We calculated infarct coordinates and depth so that the infarct tissue can be sampled without staining. For more precision, the ischemic cortex can be delimited staining its surface before sectioning and cortical tissue into which TTC diffuses can be afterwards discarded, as we had previously measured the TTC diffusion depth in rat brains.</description><identifier>ISSN: 0885-7490</identifier><identifier>EISSN: 1573-7365</identifier><identifier>DOI: 10.1007/s11011-012-9359-x</identifier><identifier>PMID: 23160835</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animal models ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Brain ; Cerebral blood flow ; Cerebral infarction ; Chloride ; Colorimetry ; Cortex ; Diffusion ; Disease Models, Animal ; Infarction, Middle Cerebral Artery - diagnosis ; Ischemia ; Male ; Metabolic Diseases ; Metabolic disorders ; Neurology ; Neurosciences ; Oncology ; Original Paper ; Rats ; Rats, Sprague-Dawley ; Sectioning ; Staining and Labeling ; Stroke ; Tetrazolium Salts</subject><ispartof>Metabolic brain disease, 2013-03, Vol.28 (1), p.21-24</ispartof><rights>Springer Science+Business Media New York 2012</rights><rights>Springer Science+Business Media New York 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-57ef51864fcb0633a834e7a466a65125e152fc8abeee80b7d90a57c8100313f73</citedby><cites>FETCH-LOGICAL-c405t-57ef51864fcb0633a834e7a466a65125e152fc8abeee80b7d90a57c8100313f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11011-012-9359-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11011-012-9359-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23160835$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ruiz-Crespo, Silvia</creatorcontrib><creatorcontrib>Trejo-Gabriel-Galán, Jose M.</creatorcontrib><creatorcontrib>Coma-del-Corral, Maria Jesus</creatorcontrib><title>Localizing coordinates of cerebral ischemic tissue without the need of staining in a rat model of focal cerebral infarct</title><title>Metabolic brain disease</title><addtitle>Metab Brain Dis</addtitle><addtitle>Metab Brain Dis</addtitle><description>Biochemical and metabolic analysis of ischemic cerebral tissue is central in stroke investigation and is usually performed in animal stroke models, such as the permanent occlusion of the middle cerebral artery (MCAO) in the rat that we have used. To be sure that the sample is from infarct tissue, it is differentiated from the surrounding normal tissue by staining, usually with 2,3,5-triphenyltetrazolium chloride (TTC), but staining can hamper biochemical colorimetric analysis. We performed this study to avoid this obstacle. A cerebral infarct was provoked in a sample of 10 rats and the brain was cut in coronal sections that were stained with TTC so that the unstained, infarct areas could be delineated in a template of each section in which areas with infarct in all animals were delineated. We calculated infarct coordinates and depth so that the infarct tissue can be sampled without staining. For more precision, the ischemic cortex can be delimited staining its surface before sectioning and cortical tissue into which TTC diffuses can be afterwards discarded, as we had previously measured the TTC diffusion depth in rat brains.</description><subject>Animal models</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brain</subject><subject>Cerebral blood flow</subject><subject>Cerebral infarction</subject><subject>Chloride</subject><subject>Colorimetry</subject><subject>Cortex</subject><subject>Diffusion</subject><subject>Disease Models, Animal</subject><subject>Infarction, Middle Cerebral Artery - diagnosis</subject><subject>Ischemia</subject><subject>Male</subject><subject>Metabolic Diseases</subject><subject>Metabolic disorders</subject><subject>Neurology</subject><subject>Neurosciences</subject><subject>Oncology</subject><subject>Original Paper</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Sectioning</subject><subject>Staining and Labeling</subject><subject>Stroke</subject><subject>Tetrazolium Salts</subject><issn>0885-7490</issn><issn>1573-7365</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc9rFTEQx4NY7LP6B3iRgBcvq5nNZjc5SvFH4YEXew7Z7KQvZTepSRaf_evN8qoUQfA0h_nMZ5j5EvIK2DtgbHifARhAw6BtFBeqOT4hOxADbwbei6dkx6QUzdApdk6e53zLGOMC1DNy3nLomeRiR477aM3s7324oTbGNPlgCmYaHbWYcExmpj7bAy7e0uJzXpH-8OUQ10LLAWlAnDY4F-PDJvGBGppMoUuccN5abtvwyBacSba8IGfOzBlfPtQLcv3p47fLL83-6-eryw_7xnZMlEYM6ATIvnN2ZD3nRvIOB9P1vekFtAJBtM5KMyKiZOMwKWbEYGX9DwfuBn5B3p68dyl-XzEXvdR7cJ5NwLhmDa2qv1BSqf9AZVdprnhF3_yF3sY1hXrIRnHRQsugUnCibIo5J3T6LvnFpJ8amN4S1KcEdU1QbwnqY515_WBexwWnPxO_I6tAewJybYUbTI9W_9P6C_Iqpl0</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Ruiz-Crespo, Silvia</creator><creator>Trejo-Gabriel-Galán, Jose M.</creator><creator>Coma-del-Corral, Maria Jesus</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20130301</creationdate><title>Localizing coordinates of cerebral ischemic tissue without the need of staining in a rat model of focal cerebral infarct</title><author>Ruiz-Crespo, Silvia ; Trejo-Gabriel-Galán, Jose M. ; Coma-del-Corral, Maria Jesus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-57ef51864fcb0633a834e7a466a65125e152fc8abeee80b7d90a57c8100313f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Brain</topic><topic>Cerebral blood flow</topic><topic>Cerebral infarction</topic><topic>Chloride</topic><topic>Colorimetry</topic><topic>Cortex</topic><topic>Diffusion</topic><topic>Disease Models, Animal</topic><topic>Infarction, Middle Cerebral Artery - diagnosis</topic><topic>Ischemia</topic><topic>Male</topic><topic>Metabolic Diseases</topic><topic>Metabolic disorders</topic><topic>Neurology</topic><topic>Neurosciences</topic><topic>Oncology</topic><topic>Original Paper</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Sectioning</topic><topic>Staining and Labeling</topic><topic>Stroke</topic><topic>Tetrazolium Salts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ruiz-Crespo, Silvia</creatorcontrib><creatorcontrib>Trejo-Gabriel-Galán, Jose M.</creatorcontrib><creatorcontrib>Coma-del-Corral, Maria Jesus</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Metabolic brain disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ruiz-Crespo, Silvia</au><au>Trejo-Gabriel-Galán, Jose M.</au><au>Coma-del-Corral, Maria Jesus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localizing coordinates of cerebral ischemic tissue without the need of staining in a rat model of focal cerebral infarct</atitle><jtitle>Metabolic brain disease</jtitle><stitle>Metab Brain Dis</stitle><addtitle>Metab Brain Dis</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>28</volume><issue>1</issue><spage>21</spage><epage>24</epage><pages>21-24</pages><issn>0885-7490</issn><eissn>1573-7365</eissn><abstract>Biochemical and metabolic analysis of ischemic cerebral tissue is central in stroke investigation and is usually performed in animal stroke models, such as the permanent occlusion of the middle cerebral artery (MCAO) in the rat that we have used. To be sure that the sample is from infarct tissue, it is differentiated from the surrounding normal tissue by staining, usually with 2,3,5-triphenyltetrazolium chloride (TTC), but staining can hamper biochemical colorimetric analysis. We performed this study to avoid this obstacle. A cerebral infarct was provoked in a sample of 10 rats and the brain was cut in coronal sections that were stained with TTC so that the unstained, infarct areas could be delineated in a template of each section in which areas with infarct in all animals were delineated. We calculated infarct coordinates and depth so that the infarct tissue can be sampled without staining. For more precision, the ischemic cortex can be delimited staining its surface before sectioning and cortical tissue into which TTC diffuses can be afterwards discarded, as we had previously measured the TTC diffusion depth in rat brains.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23160835</pmid><doi>10.1007/s11011-012-9359-x</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0885-7490 |
| ispartof | Metabolic brain disease, 2013-03, Vol.28 (1), p.21-24 |
| issn | 0885-7490 1573-7365 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1291609899 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animal models Animals Biochemistry Biomedical and Life Sciences Biomedicine Brain Cerebral blood flow Cerebral infarction Chloride Colorimetry Cortex Diffusion Disease Models, Animal Infarction, Middle Cerebral Artery - diagnosis Ischemia Male Metabolic Diseases Metabolic disorders Neurology Neurosciences Oncology Original Paper Rats Rats, Sprague-Dawley Sectioning Staining and Labeling Stroke Tetrazolium Salts |
| title | Localizing coordinates of cerebral ischemic tissue without the need of staining in a rat model of focal cerebral infarct |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T02%3A25%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Localizing%20coordinates%20of%20cerebral%20ischemic%20tissue%20without%20the%20need%20of%20staining%20in%20a%20rat%20model%20of%20focal%20cerebral%20infarct&rft.jtitle=Metabolic%20brain%20disease&rft.au=Ruiz-Crespo,%20Silvia&rft.date=2013-03-01&rft.volume=28&rft.issue=1&rft.spage=21&rft.epage=24&rft.pages=21-24&rft.issn=0885-7490&rft.eissn=1573-7365&rft_id=info:doi/10.1007/s11011-012-9359-x&rft_dat=%3Cproquest_cross%3E2881433321%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1283521201&rft_id=info:pmid/23160835&rfr_iscdi=true |