Bioenergetics for the growth of Staphylococcus lentus in biocompatible choline salts
Choline-based biocompatible salts were used as “nutrients” for the growth of Staphylococcus lentus bacteria. Increase in the growth rate of bacteria was observed, compared to conventional carbon sources. In the case of the ionic liquid, choline lactate, the increase was pronounced. Bacterial growth...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2013-02, Vol.97 (4), p.1767-1774 |
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| creator | Sekar, Sudharshan Mahadevan, Surianarayanan Vijayaraghavan, Ranganathan Mandal, Asit Baran MacFarlane, D. R. |
| description | Choline-based biocompatible salts were used as “nutrients” for the growth of
Staphylococcus lentus
bacteria. Increase in the growth rate of bacteria was observed, compared to conventional carbon sources. In the case of the ionic liquid, choline lactate, the increase was pronounced. Bacterial growth was correlated with power–time curve in an investigation monitored online by reaction calorimetry. From the power–time curve, three phases of the growth can be distinctly seen. Heat yield coefficients estimated for the growth of
S. lentus
were found to match well with those reported hitherto. A comparative study of heat yields (catabolic) between glucose and choline lactate revealed significant information; the heat yield due to choline lactate (
Y
Q/S
) consumption and oxygen (
Y
Q/O
) were 23.4 kJ/g and 435 kJ/mol and whereas that for glucose with oxygen were 9.6 kJ/g and 427 kJ/mol, respectively, showing clearly the preferential affinity of choline lactate by the bacteria rather than glucose. This study also established that the use of ionic liquids as nutrients can be monitored using bioreaction calorimetry. |
| doi_str_mv | 10.1007/s00253-012-4009-0 |
| format | Article |
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Staphylococcus lentus
bacteria. Increase in the growth rate of bacteria was observed, compared to conventional carbon sources. In the case of the ionic liquid, choline lactate, the increase was pronounced. Bacterial growth was correlated with power–time curve in an investigation monitored online by reaction calorimetry. From the power–time curve, three phases of the growth can be distinctly seen. Heat yield coefficients estimated for the growth of
S. lentus
were found to match well with those reported hitherto. A comparative study of heat yields (catabolic) between glucose and choline lactate revealed significant information; the heat yield due to choline lactate (
Y
Q/S
) consumption and oxygen (
Y
Q/O
) were 23.4 kJ/g and 435 kJ/mol and whereas that for glucose with oxygen were 9.6 kJ/g and 427 kJ/mol, respectively, showing clearly the preferential affinity of choline lactate by the bacteria rather than glucose. This study also established that the use of ionic liquids as nutrients can be monitored using bioreaction calorimetry.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-012-4009-0</identifier><identifier>PMID: 22526779</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Bacteria ; Biocompatibility ; Bioenergetics ; Biomass ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Calorimetry ; Carbon ; Carbon sources ; Choline ; Choline - metabolism ; Comparative studies ; Energy Metabolism ; Environmental Biotechnology ; Fourier transforms ; Fungi ; Glucose ; Glucose - metabolism ; Glycerol ; Hot Temperature ; Ionic liquids ; Ions ; Kinetics ; Lactates ; Life Sciences ; Metabolism ; Metabolites ; Methods ; Microbial Genetics and Genomics ; Microbiological research ; Microbiology ; Nutrients ; Physiological aspects ; Physiology ; Salt ; Staphylococcus ; Staphylococcus - chemistry ; Staphylococcus - genetics ; Staphylococcus - growth & development ; Staphylococcus - metabolism ; Streptococcus infections ; Studies ; Toxicity</subject><ispartof>Applied microbiology and biotechnology, 2013-02, Vol.97 (4), p.1767-1774</ispartof><rights>Springer-Verlag 2012</rights><rights>COPYRIGHT 2013 Springer</rights><rights>Springer-Verlag 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c543t-e18ced84f1ca7c3dc62d1258f59815e1d4447665c58f1b798588db73dac6b35e3</citedby><cites>FETCH-LOGICAL-c543t-e18ced84f1ca7c3dc62d1258f59815e1d4447665c58f1b798588db73dac6b35e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-012-4009-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-012-4009-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22526779$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sekar, Sudharshan</creatorcontrib><creatorcontrib>Mahadevan, Surianarayanan</creatorcontrib><creatorcontrib>Vijayaraghavan, Ranganathan</creatorcontrib><creatorcontrib>Mandal, Asit Baran</creatorcontrib><creatorcontrib>MacFarlane, D. R.</creatorcontrib><title>Bioenergetics for the growth of Staphylococcus lentus in biocompatible choline salts</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Choline-based biocompatible salts were used as “nutrients” for the growth of
Staphylococcus lentus
bacteria. Increase in the growth rate of bacteria was observed, compared to conventional carbon sources. In the case of the ionic liquid, choline lactate, the increase was pronounced. Bacterial growth was correlated with power–time curve in an investigation monitored online by reaction calorimetry. From the power–time curve, three phases of the growth can be distinctly seen. Heat yield coefficients estimated for the growth of
S. lentus
were found to match well with those reported hitherto. A comparative study of heat yields (catabolic) between glucose and choline lactate revealed significant information; the heat yield due to choline lactate (
Y
Q/S
) consumption and oxygen (
Y
Q/O
) were 23.4 kJ/g and 435 kJ/mol and whereas that for glucose with oxygen were 9.6 kJ/g and 427 kJ/mol, respectively, showing clearly the preferential affinity of choline lactate by the bacteria rather than glucose. This study also established that the use of ionic liquids as nutrients can be monitored using bioreaction calorimetry.</description><subject>Bacteria</subject><subject>Biocompatibility</subject><subject>Bioenergetics</subject><subject>Biomass</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Calorimetry</subject><subject>Carbon</subject><subject>Carbon sources</subject><subject>Choline</subject><subject>Choline - metabolism</subject><subject>Comparative studies</subject><subject>Energy Metabolism</subject><subject>Environmental Biotechnology</subject><subject>Fourier transforms</subject><subject>Fungi</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>Glycerol</subject><subject>Hot Temperature</subject><subject>Ionic liquids</subject><subject>Ions</subject><subject>Kinetics</subject><subject>Lactates</subject><subject>Life Sciences</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Methods</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiological research</subject><subject>Microbiology</subject><subject>Nutrients</subject><subject>Physiological aspects</subject><subject>Physiology</subject><subject>Salt</subject><subject>Staphylococcus</subject><subject>Staphylococcus - chemistry</subject><subject>Staphylococcus - genetics</subject><subject>Staphylococcus - growth & development</subject><subject>Staphylococcus - metabolism</subject><subject>Streptococcus infections</subject><subject>Studies</subject><subject>Toxicity</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkl9rFTEQxYNY7LX6AXyRBV_0YWv-J_vYllYLBcHW55DNzu5N2d1ckyzab28ut9XeoiB5GJj5nWEOOQi9IfiYYKw-JoypYDUmtOYYNzV-hlaEM1pjSfhztMJEiVqJRh-ilynd4gJqKV-gQ0oFlUo1K3Rz6gPMEAfI3qWqD7HKa6iGGH7kdRX66jrbzfpuDC44t6RqhDmX4ueq9aU3bWz27QiVW4fRz1AlO-b0Ch30dkzw-r4eoW8X5zdnn-urL58uz06uaic4yzUQ7aDTvCfOKsc6J2lHqNB9uZgIIB3nXEkpXGmRVjVaaN21inXWyZYJYEfo_W7vJobvC6RsJp8cjKOdISzJENoQiTXn5D9QzaluJG4K-u4JehuWOBcjW4oJyhlTf6jBjmD83IccrdsuNSdlLokmVBbq-C9UeR1M3oUZel_6e4IPe4LCZPiZB7ukZC6vv-6zZMe6GFKK0JtN9JONd4Zgsw2I2QXElH8324AYXDRv780t7QTdb8VDIgpAd0Aqo3mA-Mj9P7f-As2ewmA</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Sekar, Sudharshan</creator><creator>Mahadevan, Surianarayanan</creator><creator>Vijayaraghavan, Ranganathan</creator><creator>Mandal, Asit Baran</creator><creator>MacFarlane, D. 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R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bioenergetics for the growth of Staphylococcus lentus in biocompatible choline salts</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>97</volume><issue>4</issue><spage>1767</spage><epage>1774</epage><pages>1767-1774</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>Choline-based biocompatible salts were used as “nutrients” for the growth of
Staphylococcus lentus
bacteria. Increase in the growth rate of bacteria was observed, compared to conventional carbon sources. In the case of the ionic liquid, choline lactate, the increase was pronounced. Bacterial growth was correlated with power–time curve in an investigation monitored online by reaction calorimetry. From the power–time curve, three phases of the growth can be distinctly seen. Heat yield coefficients estimated for the growth of
S. lentus
were found to match well with those reported hitherto. A comparative study of heat yields (catabolic) between glucose and choline lactate revealed significant information; the heat yield due to choline lactate (
Y
Q/S
) consumption and oxygen (
Y
Q/O
) were 23.4 kJ/g and 435 kJ/mol and whereas that for glucose with oxygen were 9.6 kJ/g and 427 kJ/mol, respectively, showing clearly the preferential affinity of choline lactate by the bacteria rather than glucose. This study also established that the use of ionic liquids as nutrients can be monitored using bioreaction calorimetry.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>22526779</pmid><doi>10.1007/s00253-012-4009-0</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2013-02, Vol.97 (4), p.1767-1774 |
| issn | 0175-7598 1432-0614 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Bacteria Biocompatibility Bioenergetics Biomass Biomedical and Life Sciences Biosynthesis Biotechnology Calorimetry Carbon Carbon sources Choline Choline - metabolism Comparative studies Energy Metabolism Environmental Biotechnology Fourier transforms Fungi Glucose Glucose - metabolism Glycerol Hot Temperature Ionic liquids Ions Kinetics Lactates Life Sciences Metabolism Metabolites Methods Microbial Genetics and Genomics Microbiological research Microbiology Nutrients Physiological aspects Physiology Salt Staphylococcus Staphylococcus - chemistry Staphylococcus - genetics Staphylococcus - growth & development Staphylococcus - metabolism Streptococcus infections Studies Toxicity |
| title | Bioenergetics for the growth of Staphylococcus lentus in biocompatible choline salts |
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