A SYBR Green-based real-time RT-PCR assay for simple and rapid detection and differentiation of highly pathogenic and classical type 2 porcine reproductive and respiratory syndrome virus circulating in China

SYBR Green coupled to melting curve analysis has been suggested to detect RNA viruses showing high genomic variability. Here, a SYBR Green-based real-time RT-PCR assay was developed for simultaneous detection and differentiation of highly pathogenic porcine reproductive and respiratory syndrome viru...

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Veröffentlicht in:	Archives of virology 2013-02, Vol.158 (2), p.407-415
Hauptverfasser:	Chai, Zheng, Ma, Wenjun, Fu, Fang, Lang, Yuekun, Wang, Wei, Tong, Guangzhi, Liu, Qinfang, Cai, Xuehui, Li, Xi
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biomedical and Life Sciences Biomedicine Cell culture China Foot & mouth disease Genes Genomes Hogs Infectious Diseases Medical Microbiology Molecular Diagnostic Techniques - economics Molecular Diagnostic Techniques - methods Organic Chemicals - metabolism Original Article Pathogens Porcine Reproductive and Respiratory Syndrome - diagnosis Porcine Reproductive and Respiratory Syndrome - virology Porcine respiratory and reproductive syndrome virus Porcine respiratory and reproductive syndrome virus - genetics Porcine respiratory and reproductive syndrome virus - isolation & purification Real-Time Polymerase Chain Reaction - economics Real-Time Polymerase Chain Reaction - methods Reproducibility Research centers Sensitivity and Specificity Staining and Labeling - economics Staining and Labeling - methods Swine Time Factors Transition Temperature Veterinary medicine Veterinary Medicine - economics Veterinary Medicine - methods Virology Virology - economics Virology - methods Viruses
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