Pathogenicity comparison of high- and low-virulence strains of Vibrio scophthalmi in olive flounder Paralichthys olivaceus
Vibrio scophthalmi , a bacterial pathogen of olive flounder Paralichthys olivaceus , exhibits strain-dependent virulence. No information is available on the comparative pathogenicity of different strains of V. scophthalmi toward olive flounder. In this study, high- and low-virulence strains (HVS and...
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| Veröffentlicht in: | Fisheries science 2013, Vol.79 (1), p.99-109 |
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| creator | Qiao, Guo Jang, In-Kwon Won, Kyoung Mi Woo, Sung Ho Xu, De-Hai Park, Soo Il |
| description | Vibrio scophthalmi
, a bacterial pathogen of olive flounder
Paralichthys olivaceus
, exhibits strain-dependent virulence. No information is available on the comparative pathogenicity of different strains of
V. scophthalmi
toward olive flounder. In this study, high- and low-virulence strains (HVS and LVS, respectively) were compared in terms of their pathogenic characteristics, including adhesion and survival, superoxide dismutase (SOD) activity, and extracellular products (ECP) of bacterial cells. The cell-mediated defense of macrophages from olive flounder against
V. scophthalmi
infection in vitro was also investigated. The results demonstrated that the SOD activity of the HVS was higher than that of the LVS. The number of viable cells of the HVS in serum increased by two log units after 18 h, whereas that of the LVS decreased. The number of cells of the HVS in skin mucus increased significantly while that of the LVS remained constant. The LD
50
values of the HVS and LVS ECP toward olive flounder were 10.14 and 15.99 μg protein/g fish, respectively. The ECP were positive for naphthol-AS-BI-phosphohydrolase, lipase, gelatinase, and leucine arylamidase. The extracellular O
2
−
overflow and intracellular O
2
−
concentration of macrophages induced by the HVS were lower than those induced by the LVS. Significantly more nitric oxide was produced by the HVS than by the LVS. |
| doi_str_mv | 10.1007/s12562-012-0567-4 |
| format | Article |
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, a bacterial pathogen of olive flounder
Paralichthys olivaceus
, exhibits strain-dependent virulence. No information is available on the comparative pathogenicity of different strains of
V. scophthalmi
toward olive flounder. In this study, high- and low-virulence strains (HVS and LVS, respectively) were compared in terms of their pathogenic characteristics, including adhesion and survival, superoxide dismutase (SOD) activity, and extracellular products (ECP) of bacterial cells. The cell-mediated defense of macrophages from olive flounder against
V. scophthalmi
infection in vitro was also investigated. The results demonstrated that the SOD activity of the HVS was higher than that of the LVS. The number of viable cells of the HVS in serum increased by two log units after 18 h, whereas that of the LVS decreased. The number of cells of the HVS in skin mucus increased significantly while that of the LVS remained constant. The LD
50
values of the HVS and LVS ECP toward olive flounder were 10.14 and 15.99 μg protein/g fish, respectively. The ECP were positive for naphthol-AS-BI-phosphohydrolase, lipase, gelatinase, and leucine arylamidase. The extracellular O
2
−
overflow and intracellular O
2
−
concentration of macrophages induced by the HVS were lower than those induced by the LVS. Significantly more nitric oxide was produced by the HVS than by the LVS.</description><identifier>ISSN: 0919-9268</identifier><identifier>EISSN: 1444-2906</identifier><identifier>DOI: 10.1007/s12562-012-0567-4</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Biomedical and Life Sciences ; Fish ; Fish & Wildlife Biology & Management ; Fisheries ; Fishery sciences ; Food Science ; Freshwater & Marine Ecology ; Hydrocarbons ; Life Sciences ; Marine ; Nitric oxide ; Original Article ; Overflow ; Paralichthys olivaceus ; Pathogens ; Phosphatase ; Pleuronectiformes ; Proteins ; R&D ; Research & development ; Studies ; Vibrio scophthalmi ; Virulence</subject><ispartof>Fisheries science, 2013, Vol.79 (1), p.99-109</ispartof><rights>The Japanese Society of Fisheries Science 2012</rights><rights>The Japanese Society of Fisheries Science 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-de8364b8b32a38ae4ab3c6762b6aff16bdbc0b26eaacf74adcb14db87c14721d3</citedby><cites>FETCH-LOGICAL-c376t-de8364b8b32a38ae4ab3c6762b6aff16bdbc0b26eaacf74adcb14db87c14721d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12562-012-0567-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12562-012-0567-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,782,786,27931,27932,41495,42564,51326</link.rule.ids></links><search><creatorcontrib>Qiao, Guo</creatorcontrib><creatorcontrib>Jang, In-Kwon</creatorcontrib><creatorcontrib>Won, Kyoung Mi</creatorcontrib><creatorcontrib>Woo, Sung Ho</creatorcontrib><creatorcontrib>Xu, De-Hai</creatorcontrib><creatorcontrib>Park, Soo Il</creatorcontrib><title>Pathogenicity comparison of high- and low-virulence strains of Vibrio scophthalmi in olive flounder Paralichthys olivaceus</title><title>Fisheries science</title><addtitle>Fish Sci</addtitle><description>Vibrio scophthalmi
, a bacterial pathogen of olive flounder
Paralichthys olivaceus
, exhibits strain-dependent virulence. No information is available on the comparative pathogenicity of different strains of
V. scophthalmi
toward olive flounder. In this study, high- and low-virulence strains (HVS and LVS, respectively) were compared in terms of their pathogenic characteristics, including adhesion and survival, superoxide dismutase (SOD) activity, and extracellular products (ECP) of bacterial cells. The cell-mediated defense of macrophages from olive flounder against
V. scophthalmi
infection in vitro was also investigated. The results demonstrated that the SOD activity of the HVS was higher than that of the LVS. The number of viable cells of the HVS in serum increased by two log units after 18 h, whereas that of the LVS decreased. The number of cells of the HVS in skin mucus increased significantly while that of the LVS remained constant. The LD
50
values of the HVS and LVS ECP toward olive flounder were 10.14 and 15.99 μg protein/g fish, respectively. The ECP were positive for naphthol-AS-BI-phosphohydrolase, lipase, gelatinase, and leucine arylamidase. The extracellular O
2
−
overflow and intracellular O
2
−
concentration of macrophages induced by the HVS were lower than those induced by the LVS. Significantly more nitric oxide was produced by the HVS than by the LVS.</description><subject>Biomedical and Life Sciences</subject><subject>Fish</subject><subject>Fish & Wildlife Biology & Management</subject><subject>Fisheries</subject><subject>Fishery sciences</subject><subject>Food Science</subject><subject>Freshwater & Marine Ecology</subject><subject>Hydrocarbons</subject><subject>Life Sciences</subject><subject>Marine</subject><subject>Nitric oxide</subject><subject>Original Article</subject><subject>Overflow</subject><subject>Paralichthys olivaceus</subject><subject>Pathogens</subject><subject>Phosphatase</subject><subject>Pleuronectiformes</subject><subject>Proteins</subject><subject>R&D</subject><subject>Research & development</subject><subject>Studies</subject><subject>Vibrio 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comparison of high- and low-virulence strains of Vibrio scophthalmi in olive flounder Paralichthys olivaceus</atitle><jtitle>Fisheries science</jtitle><stitle>Fish Sci</stitle><date>2013</date><risdate>2013</risdate><volume>79</volume><issue>1</issue><spage>99</spage><epage>109</epage><pages>99-109</pages><issn>0919-9268</issn><eissn>1444-2906</eissn><abstract>Vibrio scophthalmi
, a bacterial pathogen of olive flounder
Paralichthys olivaceus
, exhibits strain-dependent virulence. No information is available on the comparative pathogenicity of different strains of
V. scophthalmi
toward olive flounder. In this study, high- and low-virulence strains (HVS and LVS, respectively) were compared in terms of their pathogenic characteristics, including adhesion and survival, superoxide dismutase (SOD) activity, and extracellular products (ECP) of bacterial cells. The cell-mediated defense of macrophages from olive flounder against
V. scophthalmi
infection in vitro was also investigated. The results demonstrated that the SOD activity of the HVS was higher than that of the LVS. The number of viable cells of the HVS in serum increased by two log units after 18 h, whereas that of the LVS decreased. The number of cells of the HVS in skin mucus increased significantly while that of the LVS remained constant. The LD
50
values of the HVS and LVS ECP toward olive flounder were 10.14 and 15.99 μg protein/g fish, respectively. The ECP were positive for naphthol-AS-BI-phosphohydrolase, lipase, gelatinase, and leucine arylamidase. The extracellular O
2
−
overflow and intracellular O
2
−
concentration of macrophages induced by the HVS were lower than those induced by the LVS. Significantly more nitric oxide was produced by the HVS than by the LVS.</abstract><cop>Japan</cop><pub>Springer Japan</pub><doi>10.1007/s12562-012-0567-4</doi><tpages>11</tpages></addata></record> |
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| ispartof | Fisheries science, 2013, Vol.79 (1), p.99-109 |
| issn | 0919-9268 1444-2906 |
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| source | SpringerNature Journals |
| subjects | Biomedical and Life Sciences Fish Fish & Wildlife Biology & Management Fisheries Fishery sciences Food Science Freshwater & Marine Ecology Hydrocarbons Life Sciences Marine Nitric oxide Original Article Overflow Paralichthys olivaceus Pathogens Phosphatase Pleuronectiformes Proteins R&D Research & development Studies Vibrio scophthalmi Virulence |
| title | Pathogenicity comparison of high- and low-virulence strains of Vibrio scophthalmi in olive flounder Paralichthys olivaceus |
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