rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells
► Live or fixed colonies of human ESC/iPSC are visualized by fluorescent dye-labeled rBC2LCN. ► rBC2LCN is highly sensitive to cell differentiation. ► Fluorescent dye-labeled rBC2LCN facilitates separation ESC/iPSC from a mixed cell population. Cell surface biomarkers have been applied to discrimina...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2013-02, Vol.431 (3), p.524-529 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Onuma, Yasuko Tateno, Hiroaki Hirabayashi, Jun Ito, Yuzuru Asashima, Makoto |
| description | ► Live or fixed colonies of human ESC/iPSC are visualized by fluorescent dye-labeled rBC2LCN. ► rBC2LCN is highly sensitive to cell differentiation. ► Fluorescent dye-labeled rBC2LCN facilitates separation ESC/iPSC from a mixed cell population.
Cell surface biomarkers have been applied to discriminate pluripotent human embryonic stem cells and induced pluripotent stem cells from differentiated cells. Here, we demonstrate that a recombinant lectin probe, rBC2LCN, a new tool for fluorescence-based imaging and flow cytometry analysis of pluripotent stem cells, is an alternative to conventional pluripotent maker antibodies. Live or fixed colonies of both human embryonic stem cells and induced pluripotent stem cells were visualized in culture medium containing fluorescent dye-labeled rBC2LCN. Fluorescent dye-labeled rBC2LCN was also successfully used to separate live pluripotent stem cells from a mixed cell population by flow cytometry. |
| doi_str_mv | 10.1016/j.bbrc.2013.01.025 |
| format | Article |
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Cell surface biomarkers have been applied to discriminate pluripotent human embryonic stem cells and induced pluripotent stem cells from differentiated cells. Here, we demonstrate that a recombinant lectin probe, rBC2LCN, a new tool for fluorescence-based imaging and flow cytometry analysis of pluripotent stem cells, is an alternative to conventional pluripotent maker antibodies. Live or fixed colonies of both human embryonic stem cells and induced pluripotent stem cells were visualized in culture medium containing fluorescent dye-labeled rBC2LCN. Fluorescent dye-labeled rBC2LCN was also successfully used to separate live pluripotent stem cells from a mixed cell population by flow cytometry.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2013.01.025</identifier><identifier>PMID: 23321312</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Biomarkers - analysis ; Cell Line ; Cell Separation - methods ; Embryonic Stem Cells ; Flow Cytometry - methods ; Fluorescent Dyes - chemistry ; Human embryonic stem cell ; Human induced pluripotent stem cell ; Humans ; Induced Pluripotent Stem Cells ; Lectin ; Lectins - chemistry ; Molecular Imaging ; Molecular Probes - chemistry ; Pluripotent stem cell ; rBC2LCN ; Recombinant Proteins - chemistry</subject><ispartof>Biochemical and biophysical research communications, 2013-02, Vol.431 (3), p.524-529</ispartof><rights>2013 Elsevier Inc.</rights><rights>Copyright © 2013 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-266e1fd9b52abaff2d2aafd63ff83d8d1ebe9c06b86a5782e73237c564e791733</citedby><cites>FETCH-LOGICAL-c466t-266e1fd9b52abaff2d2aafd63ff83d8d1ebe9c06b86a5782e73237c564e791733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2013.01.025$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23321312$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Onuma, Yasuko</creatorcontrib><creatorcontrib>Tateno, Hiroaki</creatorcontrib><creatorcontrib>Hirabayashi, Jun</creatorcontrib><creatorcontrib>Ito, Yuzuru</creatorcontrib><creatorcontrib>Asashima, Makoto</creatorcontrib><title>rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>► Live or fixed colonies of human ESC/iPSC are visualized by fluorescent dye-labeled rBC2LCN. ► rBC2LCN is highly sensitive to cell differentiation. ► Fluorescent dye-labeled rBC2LCN facilitates separation ESC/iPSC from a mixed cell population.
Cell surface biomarkers have been applied to discriminate pluripotent human embryonic stem cells and induced pluripotent stem cells from differentiated cells. Here, we demonstrate that a recombinant lectin probe, rBC2LCN, a new tool for fluorescence-based imaging and flow cytometry analysis of pluripotent stem cells, is an alternative to conventional pluripotent maker antibodies. Live or fixed colonies of both human embryonic stem cells and induced pluripotent stem cells were visualized in culture medium containing fluorescent dye-labeled rBC2LCN. Fluorescent dye-labeled rBC2LCN was also successfully used to separate live pluripotent stem cells from a mixed cell population by flow cytometry.</description><subject>Biomarkers - analysis</subject><subject>Cell Line</subject><subject>Cell Separation - methods</subject><subject>Embryonic Stem Cells</subject><subject>Flow Cytometry - methods</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Human embryonic stem cell</subject><subject>Human induced pluripotent stem cell</subject><subject>Humans</subject><subject>Induced Pluripotent Stem Cells</subject><subject>Lectin</subject><subject>Lectins - chemistry</subject><subject>Molecular Imaging</subject><subject>Molecular Probes - chemistry</subject><subject>Pluripotent stem cell</subject><subject>rBC2LCN</subject><subject>Recombinant Proteins - chemistry</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kDtPwzAUhS0EouXxBxiQRwYSfO3ESSQWqHiqggUkNstxrourPIqdgPj3pBQYme7y3aNzPkKOgMXAQJ4t47L0JuYMRMwgZjzdIlNgBYs4sGSbTBljMuIFvEzIXghLxgASWeySCReCgwA-Jff-csbns4dTqmmLH3TluxKp7Tyt3TtSg3VNXaMXrl3QztLXodEtXdWDd6uux7anocfmGwsHZMfqOuDhz90nz9dXT7PbaP54cze7mEcmkbKPuJQItirKlOtSW8srrrWtpLA2F1VeAZZYGCbLXOo0yzlmgovMpDLBrIBMiH1ysskdu74NGHrVuLBuoFvshqCA53lRJGmejCjfoMZ3IXi0auXHNf5TAVNrh2qp1g7V2qFioEaH49PxT_5QNlj9vfxKG4HzDYDjyneHXgXjsDVYOY-mV1Xn_sv_AnUXgXQ</recordid><startdate>20130215</startdate><enddate>20130215</enddate><creator>Onuma, Yasuko</creator><creator>Tateno, Hiroaki</creator><creator>Hirabayashi, Jun</creator><creator>Ito, Yuzuru</creator><creator>Asashima, Makoto</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130215</creationdate><title>rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells</title><author>Onuma, Yasuko ; Tateno, Hiroaki ; Hirabayashi, Jun ; Ito, Yuzuru ; Asashima, Makoto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-266e1fd9b52abaff2d2aafd63ff83d8d1ebe9c06b86a5782e73237c564e791733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Biomarkers - analysis</topic><topic>Cell Line</topic><topic>Cell Separation - methods</topic><topic>Embryonic Stem Cells</topic><topic>Flow Cytometry - methods</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Human embryonic stem cell</topic><topic>Human induced pluripotent stem cell</topic><topic>Humans</topic><topic>Induced Pluripotent Stem Cells</topic><topic>Lectin</topic><topic>Lectins - chemistry</topic><topic>Molecular Imaging</topic><topic>Molecular Probes - chemistry</topic><topic>Pluripotent stem cell</topic><topic>rBC2LCN</topic><topic>Recombinant Proteins - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Onuma, Yasuko</creatorcontrib><creatorcontrib>Tateno, Hiroaki</creatorcontrib><creatorcontrib>Hirabayashi, Jun</creatorcontrib><creatorcontrib>Ito, Yuzuru</creatorcontrib><creatorcontrib>Asashima, Makoto</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Onuma, Yasuko</au><au>Tateno, Hiroaki</au><au>Hirabayashi, Jun</au><au>Ito, Yuzuru</au><au>Asashima, Makoto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2013-02-15</date><risdate>2013</risdate><volume>431</volume><issue>3</issue><spage>524</spage><epage>529</epage><pages>524-529</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>► Live or fixed colonies of human ESC/iPSC are visualized by fluorescent dye-labeled rBC2LCN. ► rBC2LCN is highly sensitive to cell differentiation. ► Fluorescent dye-labeled rBC2LCN facilitates separation ESC/iPSC from a mixed cell population.
Cell surface biomarkers have been applied to discriminate pluripotent human embryonic stem cells and induced pluripotent stem cells from differentiated cells. Here, we demonstrate that a recombinant lectin probe, rBC2LCN, a new tool for fluorescence-based imaging and flow cytometry analysis of pluripotent stem cells, is an alternative to conventional pluripotent maker antibodies. Live or fixed colonies of both human embryonic stem cells and induced pluripotent stem cells were visualized in culture medium containing fluorescent dye-labeled rBC2LCN. Fluorescent dye-labeled rBC2LCN was also successfully used to separate live pluripotent stem cells from a mixed cell population by flow cytometry.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23321312</pmid><doi>10.1016/j.bbrc.2013.01.025</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biomarkers - analysis Cell Line Cell Separation - methods Embryonic Stem Cells Flow Cytometry - methods Fluorescent Dyes - chemistry Human embryonic stem cell Human induced pluripotent stem cell Humans Induced Pluripotent Stem Cells Lectin Lectins - chemistry Molecular Imaging Molecular Probes - chemistry Pluripotent stem cell rBC2LCN Recombinant Proteins - chemistry |
| title | rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells |
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