Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: The roles of Hsp47 and basic fibroblast growth factor
This study was designed to assess the mechanism of obstruction in obstructing colorectal carcinomas. Thirty-five cases of obstructing colorectal carcinoma and 34 cases of non-obstructing carcinoma were studied. The lesions were immunohistochemically analyzed using antibodies for pan-cytokeratin, α-s...
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| Veröffentlicht in: | Pathology, research and practice research and practice, 2013-02, Vol.209 (2), p.69-74 |
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| creator | Xu, Chuan-Jie Mikami, Tetuo Nakamura, Takatoshi Tsuruta, Tomoko Nakada, Norihiro Yanagisawa, Nobuyuki Jiang, Shi-Xu Okayasu, Isao |
| description | This study was designed to assess the mechanism of obstruction in obstructing colorectal carcinomas. Thirty-five cases of obstructing colorectal carcinoma and 34 cases of non-obstructing carcinoma were studied. The lesions were immunohistochemically analyzed using antibodies for pan-cytokeratin, α-smooth muscle actin, matrix metalloproteinase-7, 47-kDa heat shock protein (Hsp47), basic fibroblast growth factor (bFGF), myeloperoxidase, and CD68. Compared with non-obstructing cases, obstructing carcinoma cases included lesions of poorer differentiation. A higher value of tumor budding was observed in obstructing than in non-obstructing carcinoma. A higher number of α-smooth muscle actin-positive myofibroblasts, a higher expression of Hsp47 in stromal spindle cells, and a higher expression of bFGF in inflammatory cells were also significant in obstructing carcinoma. Therefore, obstructing colon carcinomas were characterized by poorer differentiation of cancer cells, a high level of tumor budding, and stromal myofibroblast proliferation resulting in fibrosis. Correlative Hsp47 expression in fibroblasts with bFGF in inflammatory cells may contribute to stromal fibrosis. |
| doi_str_mv | 10.1016/j.prp.2012.10.008 |
| format | Article |
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Thirty-five cases of obstructing colorectal carcinoma and 34 cases of non-obstructing carcinoma were studied. The lesions were immunohistochemically analyzed using antibodies for pan-cytokeratin, α-smooth muscle actin, matrix metalloproteinase-7, 47-kDa heat shock protein (Hsp47), basic fibroblast growth factor (bFGF), myeloperoxidase, and CD68. Compared with non-obstructing cases, obstructing carcinoma cases included lesions of poorer differentiation. A higher value of tumor budding was observed in obstructing than in non-obstructing carcinoma. A higher number of α-smooth muscle actin-positive myofibroblasts, a higher expression of Hsp47 in stromal spindle cells, and a higher expression of bFGF in inflammatory cells were also significant in obstructing carcinoma. Therefore, obstructing colon carcinomas were characterized by poorer differentiation of cancer cells, a high level of tumor budding, and stromal myofibroblast proliferation resulting in fibrosis. Correlative Hsp47 expression in fibroblasts with bFGF in inflammatory cells may contribute to stromal fibrosis.</description><identifier>ISSN: 0344-0338</identifier><identifier>EISSN: 1618-0631</identifier><identifier>DOI: 10.1016/j.prp.2012.10.008</identifier><identifier>PMID: 23265436</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Aged ; Basic fibroblast growth factor ; Carcinoma - complications ; Carcinoma - metabolism ; Carcinoma - pathology ; Colon cancer ; Colonic Neoplasms - complications ; Colonic Neoplasms - metabolism ; Colonic Neoplasms - pathology ; Female ; Fibroblast Growth Factor 2 - analysis ; Fibroblast Growth Factor 2 - metabolism ; Fibrosis - pathology ; Hsp47 ; HSP47 Heat-Shock Proteins - analysis ; HSP47 Heat-Shock Proteins - metabolism ; Humans ; Immunohistochemistry ; Intestinal Obstruction - etiology ; Intestinal Obstruction - metabolism ; Male ; Myofibroblast ; Myofibroblasts - pathology ; Neoplasm Grading ; Neoplasm Staging ; Obstruction</subject><ispartof>Pathology, research and practice, 2013-02, Vol.209 (2), p.69-74</ispartof><rights>2012 Elsevier GmbH</rights><rights>Copyright © 2012 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-fb0dcd56171dabda0d6d284aa24ec41133e181ce31bc12a620adfeb989d859f43</citedby><cites>FETCH-LOGICAL-c419t-fb0dcd56171dabda0d6d284aa24ec41133e181ce31bc12a620adfeb989d859f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0344033812002981$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23265436$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Chuan-Jie</creatorcontrib><creatorcontrib>Mikami, Tetuo</creatorcontrib><creatorcontrib>Nakamura, Takatoshi</creatorcontrib><creatorcontrib>Tsuruta, Tomoko</creatorcontrib><creatorcontrib>Nakada, Norihiro</creatorcontrib><creatorcontrib>Yanagisawa, Nobuyuki</creatorcontrib><creatorcontrib>Jiang, Shi-Xu</creatorcontrib><creatorcontrib>Okayasu, Isao</creatorcontrib><title>Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: The roles of Hsp47 and basic fibroblast growth factor</title><title>Pathology, research and practice</title><addtitle>Pathol Res Pract</addtitle><description>This study was designed to assess the mechanism of obstruction in obstructing colorectal carcinomas. Thirty-five cases of obstructing colorectal carcinoma and 34 cases of non-obstructing carcinoma were studied. The lesions were immunohistochemically analyzed using antibodies for pan-cytokeratin, α-smooth muscle actin, matrix metalloproteinase-7, 47-kDa heat shock protein (Hsp47), basic fibroblast growth factor (bFGF), myeloperoxidase, and CD68. Compared with non-obstructing cases, obstructing carcinoma cases included lesions of poorer differentiation. A higher value of tumor budding was observed in obstructing than in non-obstructing carcinoma. A higher number of α-smooth muscle actin-positive myofibroblasts, a higher expression of Hsp47 in stromal spindle cells, and a higher expression of bFGF in inflammatory cells were also significant in obstructing carcinoma. Therefore, obstructing colon carcinomas were characterized by poorer differentiation of cancer cells, a high level of tumor budding, and stromal myofibroblast proliferation resulting in fibrosis. Correlative Hsp47 expression in fibroblasts with bFGF in inflammatory cells may contribute to stromal fibrosis.</description><subject>Aged</subject><subject>Basic fibroblast growth factor</subject><subject>Carcinoma - complications</subject><subject>Carcinoma - metabolism</subject><subject>Carcinoma - pathology</subject><subject>Colon cancer</subject><subject>Colonic Neoplasms - complications</subject><subject>Colonic Neoplasms - metabolism</subject><subject>Colonic Neoplasms - pathology</subject><subject>Female</subject><subject>Fibroblast Growth Factor 2 - analysis</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>Fibrosis - pathology</subject><subject>Hsp47</subject><subject>HSP47 Heat-Shock Proteins - analysis</subject><subject>HSP47 Heat-Shock Proteins - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Intestinal Obstruction - etiology</subject><subject>Intestinal Obstruction - metabolism</subject><subject>Male</subject><subject>Myofibroblast</subject><subject>Myofibroblasts - pathology</subject><subject>Neoplasm Grading</subject><subject>Neoplasm Staging</subject><subject>Obstruction</subject><issn>0344-0338</issn><issn>1618-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kb1uFDEUhS0EIkvgAWiQS4rM4mt7vR6oUAQkUqQ0m9ryb-LVzHiwPUF5CZ4ZbzYgqlSWr893ro4PQu-BrIGA-LRfz3leUwK03deEyBdoBQJkRwSDl2hFGOcdYUyeoDel7AkhW8LhNTqhjIoNZ2KFfu-WMWVsFufidHuGx4cUosnJDLpUPOc0xOCzrjFNZ1hPDj--llhwnHAypebF1kZim4Y0YauzjVMa9We8u_O44b7gFPBFmfn2kTe6RIv_23Gb0696h4O2NeW36FXQQ_Hvns5TdPP92-78oru6_nF5_vWqsxz62gVDnHUbAVtw2jhNnHBUcq0p900BjHmQYD0DY4FqQYl2wZte9k5u-sDZKfp49G0Jfy6-VDXGYv0w6MmnpSigUvY93QrWpHCU2pa7ZB_UnOOo84MCog41qH2bzOpQw2HUamjMhyf7xYze_SP-_nsTfDkKfAt5H31WxUY_We9i9rYql-Iz9n8At9Sbbg</recordid><startdate>20130215</startdate><enddate>20130215</enddate><creator>Xu, Chuan-Jie</creator><creator>Mikami, Tetuo</creator><creator>Nakamura, Takatoshi</creator><creator>Tsuruta, Tomoko</creator><creator>Nakada, Norihiro</creator><creator>Yanagisawa, Nobuyuki</creator><creator>Jiang, Shi-Xu</creator><creator>Okayasu, Isao</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130215</creationdate><title>Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: The roles of Hsp47 and basic fibroblast growth factor</title><author>Xu, Chuan-Jie ; Mikami, Tetuo ; Nakamura, Takatoshi ; Tsuruta, Tomoko ; Nakada, Norihiro ; Yanagisawa, Nobuyuki ; Jiang, Shi-Xu ; Okayasu, Isao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-fb0dcd56171dabda0d6d284aa24ec41133e181ce31bc12a620adfeb989d859f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Aged</topic><topic>Basic fibroblast growth factor</topic><topic>Carcinoma - complications</topic><topic>Carcinoma - metabolism</topic><topic>Carcinoma - pathology</topic><topic>Colon cancer</topic><topic>Colonic Neoplasms - complications</topic><topic>Colonic Neoplasms - metabolism</topic><topic>Colonic Neoplasms - pathology</topic><topic>Female</topic><topic>Fibroblast Growth Factor 2 - analysis</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>Fibrosis - pathology</topic><topic>Hsp47</topic><topic>HSP47 Heat-Shock Proteins - analysis</topic><topic>HSP47 Heat-Shock Proteins - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Intestinal Obstruction - etiology</topic><topic>Intestinal Obstruction - metabolism</topic><topic>Male</topic><topic>Myofibroblast</topic><topic>Myofibroblasts - pathology</topic><topic>Neoplasm Grading</topic><topic>Neoplasm Staging</topic><topic>Obstruction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Chuan-Jie</creatorcontrib><creatorcontrib>Mikami, Tetuo</creatorcontrib><creatorcontrib>Nakamura, Takatoshi</creatorcontrib><creatorcontrib>Tsuruta, Tomoko</creatorcontrib><creatorcontrib>Nakada, Norihiro</creatorcontrib><creatorcontrib>Yanagisawa, Nobuyuki</creatorcontrib><creatorcontrib>Jiang, Shi-Xu</creatorcontrib><creatorcontrib>Okayasu, Isao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pathology, research and practice</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Chuan-Jie</au><au>Mikami, Tetuo</au><au>Nakamura, Takatoshi</au><au>Tsuruta, Tomoko</au><au>Nakada, Norihiro</au><au>Yanagisawa, Nobuyuki</au><au>Jiang, Shi-Xu</au><au>Okayasu, Isao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: The roles of Hsp47 and basic fibroblast growth factor</atitle><jtitle>Pathology, research and practice</jtitle><addtitle>Pathol Res Pract</addtitle><date>2013-02-15</date><risdate>2013</risdate><volume>209</volume><issue>2</issue><spage>69</spage><epage>74</epage><pages>69-74</pages><issn>0344-0338</issn><eissn>1618-0631</eissn><abstract>This study was designed to assess the mechanism of obstruction in obstructing colorectal carcinomas. Thirty-five cases of obstructing colorectal carcinoma and 34 cases of non-obstructing carcinoma were studied. The lesions were immunohistochemically analyzed using antibodies for pan-cytokeratin, α-smooth muscle actin, matrix metalloproteinase-7, 47-kDa heat shock protein (Hsp47), basic fibroblast growth factor (bFGF), myeloperoxidase, and CD68. Compared with non-obstructing cases, obstructing carcinoma cases included lesions of poorer differentiation. A higher value of tumor budding was observed in obstructing than in non-obstructing carcinoma. A higher number of α-smooth muscle actin-positive myofibroblasts, a higher expression of Hsp47 in stromal spindle cells, and a higher expression of bFGF in inflammatory cells were also significant in obstructing carcinoma. Therefore, obstructing colon carcinomas were characterized by poorer differentiation of cancer cells, a high level of tumor budding, and stromal myofibroblast proliferation resulting in fibrosis. 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| subjects | Aged Basic fibroblast growth factor Carcinoma - complications Carcinoma - metabolism Carcinoma - pathology Colon cancer Colonic Neoplasms - complications Colonic Neoplasms - metabolism Colonic Neoplasms - pathology Female Fibroblast Growth Factor 2 - analysis Fibroblast Growth Factor 2 - metabolism Fibrosis - pathology Hsp47 HSP47 Heat-Shock Proteins - analysis HSP47 Heat-Shock Proteins - metabolism Humans Immunohistochemistry Intestinal Obstruction - etiology Intestinal Obstruction - metabolism Male Myofibroblast Myofibroblasts - pathology Neoplasm Grading Neoplasm Staging Obstruction |
| title | Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: The roles of Hsp47 and basic fibroblast growth factor |
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