Potential application of superparamagnetic nanoparticles for extraction of bacterial genomic DNA from contaminated food and environmental samples

BACKGROUND: Isolation of high‐molecular‐weight DNA is essential for many molecular biology applications. Owing to the presence of polymerase chain reaction (PCR) inhibitors, there is a scarcity of suitable protocols for PCR‐ready DNA extraction from food and natural environments. The conventional ch...

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Veröffentlicht in:	Journal of the science of food and agriculture 2013-03, Vol.93 (4), p.788-793
Hauptverfasser:	Basu, Semanti, Chatterjee, Saptarshi, Bandyopadhyay, Arghya, Sarkar, Keka
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteria Bacteria - genetics Beverages - microbiology Chloroform Deoxyribonucleic acid DNA DNA, Bacterial - isolation & purification Environment Ferric Compounds food and environmental samples Food contamination & poisoning Food Microbiology - methods Fresh Water - microbiology Fruit - microbiology Genome, Bacterial genomic DNA Genomics Gram-Negative Bacteria - genetics Gram-Positive Bacteria - genetics magnetic nanoparticle (MNP) Magnets Milk - microbiology Molecular Weight Nanoparticles Phenols Polymerase Chain Reaction Water Microbiology
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