Potential application of superparamagnetic nanoparticles for extraction of bacterial genomic DNA from contaminated food and environmental samples
BACKGROUND: Isolation of high‐molecular‐weight DNA is essential for many molecular biology applications. Owing to the presence of polymerase chain reaction (PCR) inhibitors, there is a scarcity of suitable protocols for PCR‐ready DNA extraction from food and natural environments. The conventional ch...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2013-03, Vol.93 (4), p.788-793 |
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| creator | Basu, Semanti Chatterjee, Saptarshi Bandyopadhyay, Arghya Sarkar, Keka |
| description | BACKGROUND: Isolation of high‐molecular‐weight DNA is essential for many molecular biology applications. Owing to the presence of polymerase chain reaction (PCR) inhibitors, there is a scarcity of suitable protocols for PCR‐ready DNA extraction from food and natural environments. The conventional chemical methods of DNA extraction are time consuming and laborious and the yield is very low. Thus the aim of this research was to develop a simple, rapid, cost‐effective method of genomic DNA extraction from food (milk and fruit juice) and environmental (pond water) samples and to detect bacterial contaminants present in those samples.
RESULTS: This approach is efficient for both Gram‐positive and Gram‐negative bacteria from all the studied samples. Herein super paramagnetic bare iron oxide nanoparticles were implemented for bacterial genomic DNA isolation. The method was also compared to the conventional phenol–chloroform method in the context of quality, quantity and timing process. This method took only half an hour or less to obtain high‐molecular‐weight purified DNA from minimum bacterial contamination. Additionally, the method was directly compatible to PCR amplification.
CONCLUSION: The problem of availability of suitable generalized methods for DNA isolation from various samples including food and environmental has been solved by a nanobiotechnological approach that may prove to be extremely useful in biotechnological applications. © 2012 Society of Chemical Industry |
| doi_str_mv | 10.1002/jsfa.5798 |
| format | Article |
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RESULTS: This approach is efficient for both Gram‐positive and Gram‐negative bacteria from all the studied samples. Herein super paramagnetic bare iron oxide nanoparticles were implemented for bacterial genomic DNA isolation. The method was also compared to the conventional phenol–chloroform method in the context of quality, quantity and timing process. This method took only half an hour or less to obtain high‐molecular‐weight purified DNA from minimum bacterial contamination. Additionally, the method was directly compatible to PCR amplification.
CONCLUSION: The problem of availability of suitable generalized methods for DNA isolation from various samples including food and environmental has been solved by a nanobiotechnological approach that may prove to be extremely useful in biotechnological applications. © 2012 Society of Chemical Industry</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.5798</identifier><identifier>PMID: 22806888</identifier><identifier>CODEN: JSFAAE</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Bacteria ; Bacteria - genetics ; Beverages - microbiology ; Chloroform ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - isolation & purification ; Environment ; Ferric Compounds ; food and environmental samples ; Food contamination & poisoning ; Food Microbiology - methods ; Fresh Water - microbiology ; Fruit - microbiology ; Genome, Bacterial ; genomic DNA ; Genomics ; Gram-Negative Bacteria - genetics ; Gram-Positive Bacteria - genetics ; magnetic nanoparticle (MNP) ; Magnets ; Milk - microbiology ; Molecular Weight ; Nanoparticles ; Phenols ; Polymerase Chain Reaction ; Water Microbiology</subject><ispartof>Journal of the science of food and agriculture, 2013-03, Vol.93 (4), p.788-793</ispartof><rights>2012 Society of Chemical Industry</rights><rights>2012 Society of Chemical Industry.</rights><rights>Copyright John Wiley and Sons, Limited Mar 15, 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3918-350c24d8d9f6434dc709bfb4912d0128c70d3f0cc7725bca4a0cad48c9fbd8da3</citedby><cites>FETCH-LOGICAL-c3918-350c24d8d9f6434dc709bfb4912d0128c70d3f0cc7725bca4a0cad48c9fbd8da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.5798$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.5798$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22806888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Basu, Semanti</creatorcontrib><creatorcontrib>Chatterjee, Saptarshi</creatorcontrib><creatorcontrib>Bandyopadhyay, Arghya</creatorcontrib><creatorcontrib>Sarkar, Keka</creatorcontrib><title>Potential application of superparamagnetic nanoparticles for extraction of bacterial genomic DNA from contaminated food and environmental samples</title><title>Journal of the science of food and agriculture</title><addtitle>J. Sci. Food Agric</addtitle><description>BACKGROUND: Isolation of high‐molecular‐weight DNA is essential for many molecular biology applications. Owing to the presence of polymerase chain reaction (PCR) inhibitors, there is a scarcity of suitable protocols for PCR‐ready DNA extraction from food and natural environments. The conventional chemical methods of DNA extraction are time consuming and laborious and the yield is very low. Thus the aim of this research was to develop a simple, rapid, cost‐effective method of genomic DNA extraction from food (milk and fruit juice) and environmental (pond water) samples and to detect bacterial contaminants present in those samples.
RESULTS: This approach is efficient for both Gram‐positive and Gram‐negative bacteria from all the studied samples. Herein super paramagnetic bare iron oxide nanoparticles were implemented for bacterial genomic DNA isolation. The method was also compared to the conventional phenol–chloroform method in the context of quality, quantity and timing process. This method took only half an hour or less to obtain high‐molecular‐weight purified DNA from minimum bacterial contamination. Additionally, the method was directly compatible to PCR amplification.
CONCLUSION: The problem of availability of suitable generalized methods for DNA isolation from various samples including food and environmental has been solved by a nanobiotechnological approach that may prove to be extremely useful in biotechnological applications. © 2012 Society of Chemical Industry</description><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Beverages - microbiology</subject><subject>Chloroform</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Environment</subject><subject>Ferric Compounds</subject><subject>food and environmental samples</subject><subject>Food contamination & poisoning</subject><subject>Food Microbiology - methods</subject><subject>Fresh Water - microbiology</subject><subject>Fruit - microbiology</subject><subject>Genome, Bacterial</subject><subject>genomic DNA</subject><subject>Genomics</subject><subject>Gram-Negative Bacteria - genetics</subject><subject>Gram-Positive Bacteria - genetics</subject><subject>magnetic nanoparticle (MNP)</subject><subject>Magnets</subject><subject>Milk - microbiology</subject><subject>Molecular Weight</subject><subject>Nanoparticles</subject><subject>Phenols</subject><subject>Polymerase Chain Reaction</subject><subject>Water Microbiology</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kVtPFDEYhhsjkRW98A-YJt7gxUA7p7aXmxVQJEhAI3fNNz2QrjPt2M4o_Az_sd3swoWJVz0979MveRF6Q8kRJaQ8XicLRw0T_BlaUCJYQQglz9Eiv5VFQ-tyH71MaU0IEaJtX6D9suSk5Zwv0J-rMBk_OegxjGPvFEwueBwsTvNo4ggRBrjzZnIKe_AhX-RtbxK2IWJzP0VQj4kub03cqO6MD0NOfLhcYhvDgFXwEwzOw2R0TgaNwWts_C8Xgx_yADmUYBiz-BXas9An83q3HqBvpydfVx-Liy9nn1bLi0JVgvKiaogqa821sG1d1VoxIjrb1YKWmtCS57OuLFGKsbLpFNRAFOiaK2G7nILqAB1uvWMMP2eTJjm4pEzfgzdhTjI7uBC0YW1G3_2DrsMcfZ4uU_nDumWCZer9llIxpBSNlWN0A8QHSYnc9CQ3PclNT5l9uzPO3WD0E_lYTAaOt8Bv15uH_5vk-c3pcqcstgmXJnP_lID4Q7asYo38fnkmb1fs-rO4upWr6i_09rAL</recordid><startdate>20130315</startdate><enddate>20130315</enddate><creator>Basu, Semanti</creator><creator>Chatterjee, Saptarshi</creator><creator>Bandyopadhyay, Arghya</creator><creator>Sarkar, Keka</creator><general>John Wiley & Sons, Ltd</general><general>John Wiley and Sons, Limited</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20130315</creationdate><title>Potential application of superparamagnetic nanoparticles for extraction of bacterial genomic DNA from contaminated food and environmental samples</title><author>Basu, Semanti ; Chatterjee, Saptarshi ; Bandyopadhyay, Arghya ; Sarkar, Keka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3918-350c24d8d9f6434dc709bfb4912d0128c70d3f0cc7725bca4a0cad48c9fbd8da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Bacteria - genetics</topic><topic>Beverages - microbiology</topic><topic>Chloroform</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Environment</topic><topic>Ferric Compounds</topic><topic>food and environmental samples</topic><topic>Food contamination & poisoning</topic><topic>Food Microbiology - methods</topic><topic>Fresh Water - microbiology</topic><topic>Fruit - microbiology</topic><topic>Genome, Bacterial</topic><topic>genomic DNA</topic><topic>Genomics</topic><topic>Gram-Negative Bacteria - genetics</topic><topic>Gram-Positive Bacteria - genetics</topic><topic>magnetic nanoparticle (MNP)</topic><topic>Magnets</topic><topic>Milk - microbiology</topic><topic>Molecular Weight</topic><topic>Nanoparticles</topic><topic>Phenols</topic><topic>Polymerase Chain Reaction</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Basu, Semanti</creatorcontrib><creatorcontrib>Chatterjee, Saptarshi</creatorcontrib><creatorcontrib>Bandyopadhyay, Arghya</creatorcontrib><creatorcontrib>Sarkar, Keka</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Basu, Semanti</au><au>Chatterjee, Saptarshi</au><au>Bandyopadhyay, Arghya</au><au>Sarkar, Keka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potential application of superparamagnetic nanoparticles for extraction of bacterial genomic DNA from contaminated food and environmental samples</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J. Sci. Food Agric</addtitle><date>2013-03-15</date><risdate>2013</risdate><volume>93</volume><issue>4</issue><spage>788</spage><epage>793</epage><pages>788-793</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><coden>JSFAAE</coden><abstract>BACKGROUND: Isolation of high‐molecular‐weight DNA is essential for many molecular biology applications. Owing to the presence of polymerase chain reaction (PCR) inhibitors, there is a scarcity of suitable protocols for PCR‐ready DNA extraction from food and natural environments. The conventional chemical methods of DNA extraction are time consuming and laborious and the yield is very low. Thus the aim of this research was to develop a simple, rapid, cost‐effective method of genomic DNA extraction from food (milk and fruit juice) and environmental (pond water) samples and to detect bacterial contaminants present in those samples.
RESULTS: This approach is efficient for both Gram‐positive and Gram‐negative bacteria from all the studied samples. Herein super paramagnetic bare iron oxide nanoparticles were implemented for bacterial genomic DNA isolation. The method was also compared to the conventional phenol–chloroform method in the context of quality, quantity and timing process. This method took only half an hour or less to obtain high‐molecular‐weight purified DNA from minimum bacterial contamination. Additionally, the method was directly compatible to PCR amplification.
CONCLUSION: The problem of availability of suitable generalized methods for DNA isolation from various samples including food and environmental has been solved by a nanobiotechnological approach that may prove to be extremely useful in biotechnological applications. © 2012 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>22806888</pmid><doi>10.1002/jsfa.5798</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of the science of food and agriculture, 2013-03, Vol.93 (4), p.788-793 |
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| subjects | Animals Bacteria Bacteria - genetics Beverages - microbiology Chloroform Deoxyribonucleic acid DNA DNA, Bacterial - isolation & purification Environment Ferric Compounds food and environmental samples Food contamination & poisoning Food Microbiology - methods Fresh Water - microbiology Fruit - microbiology Genome, Bacterial genomic DNA Genomics Gram-Negative Bacteria - genetics Gram-Positive Bacteria - genetics magnetic nanoparticle (MNP) Magnets Milk - microbiology Molecular Weight Nanoparticles Phenols Polymerase Chain Reaction Water Microbiology |
| title | Potential application of superparamagnetic nanoparticles for extraction of bacterial genomic DNA from contaminated food and environmental samples |
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