Nicotine Alters MicroRNA Expression and Hinders Human Adult Stem Cell Regenerative Potential
Adult stem cells are critical for the healing process in regenerative medicine. However, cigarette smoking inhibits stem cell recruitment to tissues and delays the wound-healing process. This study investigated the effect of nicotine, a major constituent in the cigarette smoke, on the regenerative p...
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| Veröffentlicht in: | Stem cells and development 2013-03, Vol.22 (5), p.781-790 |
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| container_title | Stem cells and development |
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| creator | Ng, Tsz Kin Carballosa, Carlos M. Pelaez, Daniel Wong, Hoi Kin Choy, Kwong Wai Pang, Chi Pui Cheung, Herman S. |
| description | Adult stem cells are critical for the healing process in regenerative medicine. However, cigarette smoking inhibits stem cell recruitment to tissues and delays the wound-healing process. This study investigated the effect of nicotine, a major constituent in the cigarette smoke, on the regenerative potentials of human mesenchymal stem cells (MSC) and periodontal ligament-derived stem cells (PDLSC). The cell proliferation of 1.0 μM nicotine-treated MSC and PDLSC was significantly reduced when compared to the untreated control. Moreover, nicotine also retarded the locomotion of these adult stem cells. Furthermore, their osteogenic differentiation capabilities were reduced in the presence of nicotine as evidenced by gene expression (
RUNX2
,
ALPL
,
BGLAP
,
COL1A1
, and
COL1A2
), calcium deposition, and alkaline phosphatase activity analyses. In addition, the microRNA (miRNA) profile of nicotine-treated PDLSC was altered; suggesting miRNAs might play an important role in the nicotine effects on stem cells. This study provided the possible mechanistic explanations on stem cell-associated healing delay in cigarette smoking. |
| doi_str_mv | 10.1089/scd.2012.0434 |
| format | Article |
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RUNX2
,
ALPL
,
BGLAP
,
COL1A1
, and
COL1A2
), calcium deposition, and alkaline phosphatase activity analyses. In addition, the microRNA (miRNA) profile of nicotine-treated PDLSC was altered; suggesting miRNAs might play an important role in the nicotine effects on stem cells. This study provided the possible mechanistic explanations on stem cell-associated healing delay in cigarette smoking.</description><identifier>ISSN: 1547-3287</identifier><identifier>EISSN: 1557-8534</identifier><identifier>DOI: 10.1089/scd.2012.0434</identifier><identifier>PMID: 23030247</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Adult Stem Cells - drug effects ; Adult Stem Cells - physiology ; Alkaline Phosphatase - biosynthesis ; Cell Differentiation - drug effects ; Cell Line ; Cell Movement - drug effects ; Cell Proliferation - drug effects ; Collagen Type I - biosynthesis ; Core Binding Factor Alpha 1 Subunit - biosynthesis ; Gene Expression ; Humans ; Mesenchymal Stromal Cells - drug effects ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Nicotine - pharmacology ; Original Research Reports ; Osteocalcin - biosynthesis ; Osteogenesis - drug effects ; Periodontal Ligament - cytology ; Periodontal Ligament - drug effects ; Regeneration - drug effects ; Smoking - adverse effects ; Wound Healing - drug effects</subject><ispartof>Stem cells and development, 2013-03, Vol.22 (5), p.781-790</ispartof><rights>2013, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-ac45be1342c1ac01fcef18ae36d8e9753f8e2e7e1152b2fa1ebd7577f51f82b63</citedby><cites>FETCH-LOGICAL-c337t-ac45be1342c1ac01fcef18ae36d8e9753f8e2e7e1152b2fa1ebd7577f51f82b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23030247$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ng, Tsz Kin</creatorcontrib><creatorcontrib>Carballosa, Carlos M.</creatorcontrib><creatorcontrib>Pelaez, Daniel</creatorcontrib><creatorcontrib>Wong, Hoi Kin</creatorcontrib><creatorcontrib>Choy, Kwong Wai</creatorcontrib><creatorcontrib>Pang, Chi Pui</creatorcontrib><creatorcontrib>Cheung, Herman S.</creatorcontrib><title>Nicotine Alters MicroRNA Expression and Hinders Human Adult Stem Cell Regenerative Potential</title><title>Stem cells and development</title><addtitle>Stem Cells Dev</addtitle><description>Adult stem cells are critical for the healing process in regenerative medicine. However, cigarette smoking inhibits stem cell recruitment to tissues and delays the wound-healing process. This study investigated the effect of nicotine, a major constituent in the cigarette smoke, on the regenerative potentials of human mesenchymal stem cells (MSC) and periodontal ligament-derived stem cells (PDLSC). The cell proliferation of 1.0 μM nicotine-treated MSC and PDLSC was significantly reduced when compared to the untreated control. Moreover, nicotine also retarded the locomotion of these adult stem cells. Furthermore, their osteogenic differentiation capabilities were reduced in the presence of nicotine as evidenced by gene expression (
RUNX2
,
ALPL
,
BGLAP
,
COL1A1
, and
COL1A2
), calcium deposition, and alkaline phosphatase activity analyses. In addition, the microRNA (miRNA) profile of nicotine-treated PDLSC was altered; suggesting miRNAs might play an important role in the nicotine effects on stem cells. This study provided the possible mechanistic explanations on stem cell-associated healing delay in cigarette smoking.</description><subject>Adult Stem Cells - drug effects</subject><subject>Adult Stem Cells - physiology</subject><subject>Alkaline Phosphatase - biosynthesis</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line</subject><subject>Cell Movement - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Collagen Type I - biosynthesis</subject><subject>Core Binding Factor Alpha 1 Subunit - biosynthesis</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Nicotine - pharmacology</subject><subject>Original Research Reports</subject><subject>Osteocalcin - biosynthesis</subject><subject>Osteogenesis - drug effects</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - drug effects</subject><subject>Regeneration - drug effects</subject><subject>Smoking - adverse effects</subject><subject>Wound Healing - drug effects</subject><issn>1547-3287</issn><issn>1557-8534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EoqUwsiKPLCn-SGp3jKpCkcqHCmxIkeOckVHiFNtB8O9J1MLKdKe7R6_uHoTOKZlSIudXQVdTRiibkpSnB2hMs0wkMuPp4dCnIuFMihE6CeGdEDZjMj1GI8YJJywVY_R6b3UbrQOc1xF8wHdW-3Zzn-Pl19ZDCLZ1WLkKr6yrhv2qa5TDedXVET9FaPAC6hpv4A0ceBXtJ-DHNoKLVtWn6MioOsDZvk7Qy_XyebFK1g83t4t8nWjORUyUTrMSKE-ZpkoTajQYKhXwWSVhLjJuJDAQQGnGSmYUhbISmRAmo0aycsYn6HKXu_XtRwchFo0Nur9LOWi7UFAmJSdzyUWPJju0_zIED6bYetso_11QUgxCi15oMQgtBqE9f7GP7soGqj_612AP8B0wjJVztYUSfPwn9gedAYLA</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Ng, Tsz Kin</creator><creator>Carballosa, Carlos M.</creator><creator>Pelaez, Daniel</creator><creator>Wong, Hoi Kin</creator><creator>Choy, Kwong Wai</creator><creator>Pang, Chi Pui</creator><creator>Cheung, Herman S.</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130301</creationdate><title>Nicotine Alters MicroRNA Expression and Hinders Human Adult Stem Cell Regenerative Potential</title><author>Ng, Tsz Kin ; Carballosa, Carlos M. ; Pelaez, Daniel ; Wong, Hoi Kin ; Choy, Kwong Wai ; Pang, Chi Pui ; Cheung, Herman S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-ac45be1342c1ac01fcef18ae36d8e9753f8e2e7e1152b2fa1ebd7577f51f82b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult Stem Cells - drug effects</topic><topic>Adult Stem Cells - physiology</topic><topic>Alkaline Phosphatase - biosynthesis</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line</topic><topic>Cell Movement - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Collagen Type I - biosynthesis</topic><topic>Core Binding Factor Alpha 1 Subunit - biosynthesis</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Nicotine - pharmacology</topic><topic>Original Research Reports</topic><topic>Osteocalcin - biosynthesis</topic><topic>Osteogenesis - drug effects</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - drug effects</topic><topic>Regeneration - drug effects</topic><topic>Smoking - adverse effects</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ng, Tsz Kin</creatorcontrib><creatorcontrib>Carballosa, Carlos M.</creatorcontrib><creatorcontrib>Pelaez, Daniel</creatorcontrib><creatorcontrib>Wong, Hoi Kin</creatorcontrib><creatorcontrib>Choy, Kwong Wai</creatorcontrib><creatorcontrib>Pang, Chi Pui</creatorcontrib><creatorcontrib>Cheung, Herman S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Stem cells and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ng, Tsz Kin</au><au>Carballosa, Carlos M.</au><au>Pelaez, Daniel</au><au>Wong, Hoi Kin</au><au>Choy, Kwong Wai</au><au>Pang, Chi Pui</au><au>Cheung, Herman S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nicotine Alters MicroRNA Expression and Hinders Human Adult Stem Cell Regenerative Potential</atitle><jtitle>Stem cells and development</jtitle><addtitle>Stem Cells Dev</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>22</volume><issue>5</issue><spage>781</spage><epage>790</epage><pages>781-790</pages><issn>1547-3287</issn><eissn>1557-8534</eissn><abstract>Adult stem cells are critical for the healing process in regenerative medicine. However, cigarette smoking inhibits stem cell recruitment to tissues and delays the wound-healing process. This study investigated the effect of nicotine, a major constituent in the cigarette smoke, on the regenerative potentials of human mesenchymal stem cells (MSC) and periodontal ligament-derived stem cells (PDLSC). The cell proliferation of 1.0 μM nicotine-treated MSC and PDLSC was significantly reduced when compared to the untreated control. Moreover, nicotine also retarded the locomotion of these adult stem cells. Furthermore, their osteogenic differentiation capabilities were reduced in the presence of nicotine as evidenced by gene expression (
RUNX2
,
ALPL
,
BGLAP
,
COL1A1
, and
COL1A2
), calcium deposition, and alkaline phosphatase activity analyses. In addition, the microRNA (miRNA) profile of nicotine-treated PDLSC was altered; suggesting miRNAs might play an important role in the nicotine effects on stem cells. This study provided the possible mechanistic explanations on stem cell-associated healing delay in cigarette smoking.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>23030247</pmid><doi>10.1089/scd.2012.0434</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Stem cells and development, 2013-03, Vol.22 (5), p.781-790 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adult Stem Cells - drug effects Adult Stem Cells - physiology Alkaline Phosphatase - biosynthesis Cell Differentiation - drug effects Cell Line Cell Movement - drug effects Cell Proliferation - drug effects Collagen Type I - biosynthesis Core Binding Factor Alpha 1 Subunit - biosynthesis Gene Expression Humans Mesenchymal Stromal Cells - drug effects MicroRNAs - genetics MicroRNAs - metabolism Nicotine - pharmacology Original Research Reports Osteocalcin - biosynthesis Osteogenesis - drug effects Periodontal Ligament - cytology Periodontal Ligament - drug effects Regeneration - drug effects Smoking - adverse effects Wound Healing - drug effects |
| title | Nicotine Alters MicroRNA Expression and Hinders Human Adult Stem Cell Regenerative Potential |
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