Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110
► The marine cyanobacterium Cyanothece sp. CCY 0110 is an efficient RPS producer. ► Amount of carbohydrates released is mainly related to the number of cells (growth). ► Light is a key factor with high light intensities enhancing RPS production. ► RPS: Nine residues (two uronic acids), sulfate group...
Gespeichert in:
| Veröffentlicht in: | Carbohydrate polymers 2013-02, Vol.92 (2), p.1408-1415 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1415 |
|---|---|
| container_issue | 2 |
| container_start_page | 1408 |
| container_title | Carbohydrate polymers |
| container_volume | 92 |
| creator | Mota, Rita Guimarães, Rodrigo Büttel, Zsófia Rossi, Federico Colica, Giovanni Silva, Carla J. Santos, Carla Gales, Luís Zille, Andrea De Philippis, Roberto Pereira, Sara B. Tamagnini, Paula |
| description | ► The marine cyanobacterium Cyanothece sp. CCY 0110 is an efficient RPS producer. ► Amount of carbohydrates released is mainly related to the number of cells (growth). ► Light is a key factor with high light intensities enhancing RPS production. ► RPS: Nine residues (two uronic acids), sulfate groups, peptides, thermostable, amorphous. ► Potential for biotechnological applications.
Cyanobacterial extracellular polymeric substances (EPS) are heteropolysaccharides that possess characteristics suitable for industrial applications, notably a high number of different monomers, strong anionic nature and high hydrophobicity. However, systematic studies that unveil the conditions influencing EPS synthesis and/or its characteristics are mandatory. In this work, Cyanothece sp. CCY 0110 was used as model organism. Our results revealed that this strain is among the most efficient EPS producers, and that the amount of RPS (released polysaccharides) is mainly related to the number of cells, rather than to the amount produced by each cell. Light was the key parameter, with high light intensity enhancing significantly RPS production (reaching 1.8gL−1), especially in the presence of combined nitrogen. The data showed that RPS are composed by nine different monosaccharides (including two uronic acids), the presence of sulfate groups and peptides, and that the polymer is remarkably thermostable and amorphous in nature. |
| doi_str_mv | 10.1016/j.carbpol.2012.10.070 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1287387725</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0144861712010983</els_id><sourcerecordid>1287387725</sourcerecordid><originalsourceid>FETCH-LOGICAL-c532t-3b2505a9883884041b8dcb220bad9330a7b29918fb1a9a97c3097afd0c66ebc43</originalsourceid><addsrcrecordid>eNqFkU2r1DAUhoMo3vHqT1CzEdy05qNtkpVI8QsuKOhduAonH3UytM2YtOL4602dUZdmE3h5Tt7DE4QeU1JTQrsXh9pCMsc41oxQVrKaCHIH7agUqqK8ae6iHaFNU8mOiiv0IOcDKaej5D66YpwrRQXdIfMxRbfaJcQZw-yw3UMCu_gUfsLvMA7Y_1hK5sdxHSHhrTbuTy7B4nHpP00-4SHFCfcnmOOy99bjfKxx33_BhFLyEN0bYMz-0eW-RrdvXn_u31U3H96-71_dVLblbKm4YS1pQUnJpWxIQ4101jBGDDjFOQFhWFlaDoaCAiUsJ0rA4IjtOm9sw6_R8_O7xxS_rT4vegp5WxtmH9esKZOCSyFYW9D2jNoUc05-0McUJkgnTYne9OqDvujVm94tLnrL3JNLxWom7_5O_fFZgGcXALKFcUgw25D_cZ1iLSOicE_P3ABRw9dUmNtPpakrX9Qy0chCvDwTvij7HnzS2QY_W-9C8nbRLob_LPsLjUWkCg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1287387725</pqid></control><display><type>article</type><title>Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Mota, Rita ; Guimarães, Rodrigo ; Büttel, Zsófia ; Rossi, Federico ; Colica, Giovanni ; Silva, Carla J. ; Santos, Carla ; Gales, Luís ; Zille, Andrea ; De Philippis, Roberto ; Pereira, Sara B. ; Tamagnini, Paula</creator><creatorcontrib>Mota, Rita ; Guimarães, Rodrigo ; Büttel, Zsófia ; Rossi, Federico ; Colica, Giovanni ; Silva, Carla J. ; Santos, Carla ; Gales, Luís ; Zille, Andrea ; De Philippis, Roberto ; Pereira, Sara B. ; Tamagnini, Paula</creatorcontrib><description>► The marine cyanobacterium Cyanothece sp. CCY 0110 is an efficient RPS producer. ► Amount of carbohydrates released is mainly related to the number of cells (growth). ► Light is a key factor with high light intensities enhancing RPS production. ► RPS: Nine residues (two uronic acids), sulfate groups, peptides, thermostable, amorphous. ► Potential for biotechnological applications.
Cyanobacterial extracellular polymeric substances (EPS) are heteropolysaccharides that possess characteristics suitable for industrial applications, notably a high number of different monomers, strong anionic nature and high hydrophobicity. However, systematic studies that unveil the conditions influencing EPS synthesis and/or its characteristics are mandatory. In this work, Cyanothece sp. CCY 0110 was used as model organism. Our results revealed that this strain is among the most efficient EPS producers, and that the amount of RPS (released polysaccharides) is mainly related to the number of cells, rather than to the amount produced by each cell. Light was the key parameter, with high light intensity enhancing significantly RPS production (reaching 1.8gL−1), especially in the presence of combined nitrogen. The data showed that RPS are composed by nine different monosaccharides (including two uronic acids), the presence of sulfate groups and peptides, and that the polymer is remarkably thermostable and amorphous in nature.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2012.10.070</identifier><identifier>PMID: 23399171</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Applied sciences ; Bacterial Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Bioreactors ; Cell Proliferation ; Culture Techniques ; Cyanobacteria ; Cyanothece ; Cyanothece - cytology ; Cyanothece - growth & development ; Cyanothece - metabolism ; Exact sciences and technology ; Extracellular polymeric substances (EPS) ; Extracellular Space - metabolism ; Fundamental and applied biological sciences. Psychology ; hydrophobicity ; industrial applications ; Industry ; light intensity ; Metabolism. Enzymes ; Microbiology ; monosaccharides ; Natural polymers ; nitrogen ; peptides ; Physicochemistry of polymers ; polymers ; polysaccharides ; Polysaccharides, Bacterial - biosynthesis ; Polysaccharides, Bacterial - metabolism ; Polysaccharides, Bacterial - secretion ; Released polysaccharides (RPS) ; Starch and polysaccharides ; sulfates ; thermal stability ; uronic acids</subject><ispartof>Carbohydrate polymers, 2013-02, Vol.92 (2), p.1408-1415</ispartof><rights>2012 Elsevier Ltd</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-3b2505a9883884041b8dcb220bad9330a7b29918fb1a9a97c3097afd0c66ebc43</citedby><cites>FETCH-LOGICAL-c532t-3b2505a9883884041b8dcb220bad9330a7b29918fb1a9a97c3097afd0c66ebc43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carbpol.2012.10.070$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26925207$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23399171$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mota, Rita</creatorcontrib><creatorcontrib>Guimarães, Rodrigo</creatorcontrib><creatorcontrib>Büttel, Zsófia</creatorcontrib><creatorcontrib>Rossi, Federico</creatorcontrib><creatorcontrib>Colica, Giovanni</creatorcontrib><creatorcontrib>Silva, Carla J.</creatorcontrib><creatorcontrib>Santos, Carla</creatorcontrib><creatorcontrib>Gales, Luís</creatorcontrib><creatorcontrib>Zille, Andrea</creatorcontrib><creatorcontrib>De Philippis, Roberto</creatorcontrib><creatorcontrib>Pereira, Sara B.</creatorcontrib><creatorcontrib>Tamagnini, Paula</creatorcontrib><title>Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>► The marine cyanobacterium Cyanothece sp. CCY 0110 is an efficient RPS producer. ► Amount of carbohydrates released is mainly related to the number of cells (growth). ► Light is a key factor with high light intensities enhancing RPS production. ► RPS: Nine residues (two uronic acids), sulfate groups, peptides, thermostable, amorphous. ► Potential for biotechnological applications.
Cyanobacterial extracellular polymeric substances (EPS) are heteropolysaccharides that possess characteristics suitable for industrial applications, notably a high number of different monomers, strong anionic nature and high hydrophobicity. However, systematic studies that unveil the conditions influencing EPS synthesis and/or its characteristics are mandatory. In this work, Cyanothece sp. CCY 0110 was used as model organism. Our results revealed that this strain is among the most efficient EPS producers, and that the amount of RPS (released polysaccharides) is mainly related to the number of cells, rather than to the amount produced by each cell. Light was the key parameter, with high light intensity enhancing significantly RPS production (reaching 1.8gL−1), especially in the presence of combined nitrogen. The data showed that RPS are composed by nine different monosaccharides (including two uronic acids), the presence of sulfate groups and peptides, and that the polymer is remarkably thermostable and amorphous in nature.</description><subject>Applied sciences</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Cell Proliferation</subject><subject>Culture Techniques</subject><subject>Cyanobacteria</subject><subject>Cyanothece</subject><subject>Cyanothece - cytology</subject><subject>Cyanothece - growth & development</subject><subject>Cyanothece - metabolism</subject><subject>Exact sciences and technology</subject><subject>Extracellular polymeric substances (EPS)</subject><subject>Extracellular Space - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hydrophobicity</subject><subject>industrial applications</subject><subject>Industry</subject><subject>light intensity</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>monosaccharides</subject><subject>Natural polymers</subject><subject>nitrogen</subject><subject>peptides</subject><subject>Physicochemistry of polymers</subject><subject>polymers</subject><subject>polysaccharides</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Polysaccharides, Bacterial - metabolism</subject><subject>Polysaccharides, Bacterial - secretion</subject><subject>Released polysaccharides (RPS)</subject><subject>Starch and polysaccharides</subject><subject>sulfates</subject><subject>thermal stability</subject><subject>uronic acids</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2r1DAUhoMo3vHqT1CzEdy05qNtkpVI8QsuKOhduAonH3UytM2YtOL4602dUZdmE3h5Tt7DE4QeU1JTQrsXh9pCMsc41oxQVrKaCHIH7agUqqK8ae6iHaFNU8mOiiv0IOcDKaej5D66YpwrRQXdIfMxRbfaJcQZw-yw3UMCu_gUfsLvMA7Y_1hK5sdxHSHhrTbuTy7B4nHpP00-4SHFCfcnmOOy99bjfKxx33_BhFLyEN0bYMz-0eW-RrdvXn_u31U3H96-71_dVLblbKm4YS1pQUnJpWxIQ4101jBGDDjFOQFhWFlaDoaCAiUsJ0rA4IjtOm9sw6_R8_O7xxS_rT4vegp5WxtmH9esKZOCSyFYW9D2jNoUc05-0McUJkgnTYne9OqDvujVm94tLnrL3JNLxWom7_5O_fFZgGcXALKFcUgw25D_cZ1iLSOicE_P3ABRw9dUmNtPpakrX9Qy0chCvDwTvij7HnzS2QY_W-9C8nbRLob_LPsLjUWkCg</recordid><startdate>20130215</startdate><enddate>20130215</enddate><creator>Mota, Rita</creator><creator>Guimarães, Rodrigo</creator><creator>Büttel, Zsófia</creator><creator>Rossi, Federico</creator><creator>Colica, Giovanni</creator><creator>Silva, Carla J.</creator><creator>Santos, Carla</creator><creator>Gales, Luís</creator><creator>Zille, Andrea</creator><creator>De Philippis, Roberto</creator><creator>Pereira, Sara B.</creator><creator>Tamagnini, Paula</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130215</creationdate><title>Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110</title><author>Mota, Rita ; Guimarães, Rodrigo ; Büttel, Zsófia ; Rossi, Federico ; Colica, Giovanni ; Silva, Carla J. ; Santos, Carla ; Gales, Luís ; Zille, Andrea ; De Philippis, Roberto ; Pereira, Sara B. ; Tamagnini, Paula</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-3b2505a9883884041b8dcb220bad9330a7b29918fb1a9a97c3097afd0c66ebc43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Applied sciences</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Bioreactors</topic><topic>Cell Proliferation</topic><topic>Culture Techniques</topic><topic>Cyanobacteria</topic><topic>Cyanothece</topic><topic>Cyanothece - cytology</topic><topic>Cyanothece - growth & development</topic><topic>Cyanothece - metabolism</topic><topic>Exact sciences and technology</topic><topic>Extracellular polymeric substances (EPS)</topic><topic>Extracellular Space - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hydrophobicity</topic><topic>industrial applications</topic><topic>Industry</topic><topic>light intensity</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>monosaccharides</topic><topic>Natural polymers</topic><topic>nitrogen</topic><topic>peptides</topic><topic>Physicochemistry of polymers</topic><topic>polymers</topic><topic>polysaccharides</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><topic>Polysaccharides, Bacterial - metabolism</topic><topic>Polysaccharides, Bacterial - secretion</topic><topic>Released polysaccharides (RPS)</topic><topic>Starch and polysaccharides</topic><topic>sulfates</topic><topic>thermal stability</topic><topic>uronic acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mota, Rita</creatorcontrib><creatorcontrib>Guimarães, Rodrigo</creatorcontrib><creatorcontrib>Büttel, Zsófia</creatorcontrib><creatorcontrib>Rossi, Federico</creatorcontrib><creatorcontrib>Colica, Giovanni</creatorcontrib><creatorcontrib>Silva, Carla J.</creatorcontrib><creatorcontrib>Santos, Carla</creatorcontrib><creatorcontrib>Gales, Luís</creatorcontrib><creatorcontrib>Zille, Andrea</creatorcontrib><creatorcontrib>De Philippis, Roberto</creatorcontrib><creatorcontrib>Pereira, Sara B.</creatorcontrib><creatorcontrib>Tamagnini, Paula</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mota, Rita</au><au>Guimarães, Rodrigo</au><au>Büttel, Zsófia</au><au>Rossi, Federico</au><au>Colica, Giovanni</au><au>Silva, Carla J.</au><au>Santos, Carla</au><au>Gales, Luís</au><au>Zille, Andrea</au><au>De Philippis, Roberto</au><au>Pereira, Sara B.</au><au>Tamagnini, Paula</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2013-02-15</date><risdate>2013</risdate><volume>92</volume><issue>2</issue><spage>1408</spage><epage>1415</epage><pages>1408-1415</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>► The marine cyanobacterium Cyanothece sp. CCY 0110 is an efficient RPS producer. ► Amount of carbohydrates released is mainly related to the number of cells (growth). ► Light is a key factor with high light intensities enhancing RPS production. ► RPS: Nine residues (two uronic acids), sulfate groups, peptides, thermostable, amorphous. ► Potential for biotechnological applications.
Cyanobacterial extracellular polymeric substances (EPS) are heteropolysaccharides that possess characteristics suitable for industrial applications, notably a high number of different monomers, strong anionic nature and high hydrophobicity. However, systematic studies that unveil the conditions influencing EPS synthesis and/or its characteristics are mandatory. In this work, Cyanothece sp. CCY 0110 was used as model organism. Our results revealed that this strain is among the most efficient EPS producers, and that the amount of RPS (released polysaccharides) is mainly related to the number of cells, rather than to the amount produced by each cell. Light was the key parameter, with high light intensity enhancing significantly RPS production (reaching 1.8gL−1), especially in the presence of combined nitrogen. The data showed that RPS are composed by nine different monosaccharides (including two uronic acids), the presence of sulfate groups and peptides, and that the polymer is remarkably thermostable and amorphous in nature.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>23399171</pmid><doi>10.1016/j.carbpol.2012.10.070</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0144-8617 |
| ispartof | Carbohydrate polymers, 2013-02, Vol.92 (2), p.1408-1415 |
| issn | 0144-8617 1879-1344 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1287387725 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Applied sciences Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Bioreactors Cell Proliferation Culture Techniques Cyanobacteria Cyanothece Cyanothece - cytology Cyanothece - growth & development Cyanothece - metabolism Exact sciences and technology Extracellular polymeric substances (EPS) Extracellular Space - metabolism Fundamental and applied biological sciences. Psychology hydrophobicity industrial applications Industry light intensity Metabolism. Enzymes Microbiology monosaccharides Natural polymers nitrogen peptides Physicochemistry of polymers polymers polysaccharides Polysaccharides, Bacterial - biosynthesis Polysaccharides, Bacterial - metabolism Polysaccharides, Bacterial - secretion Released polysaccharides (RPS) Starch and polysaccharides sulfates thermal stability uronic acids |
| title | Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T20%3A28%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Production%20and%20characterization%20of%20extracellular%20carbohydrate%20polymer%20from%20Cyanothece%20sp.%20CCY%200110&rft.jtitle=Carbohydrate%20polymers&rft.au=Mota,%20Rita&rft.date=2013-02-15&rft.volume=92&rft.issue=2&rft.spage=1408&rft.epage=1415&rft.pages=1408-1415&rft.issn=0144-8617&rft.eissn=1879-1344&rft.coden=CAPOD8&rft_id=info:doi/10.1016/j.carbpol.2012.10.070&rft_dat=%3Cproquest_cross%3E1287387725%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1287387725&rft_id=info:pmid/23399171&rft_els_id=S0144861712010983&rfr_iscdi=true |