Lipid‐Labeling Facilitates a Novel Magnetic Isolation Procedure to Characterize Pathogen‐Containing Phagosomes

Here we describe a novel approach for the isolation and biochemical characterization of pathogen‐containing compartments from primary cells: We developed a lipid‐based procedure to magnetically label the surface of bacteria and visualized the label by scanning and transmission electron microscopy (S...

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Veröffentlicht in:	Traffic (Copenhagen, Denmark) Denmark), 2013-03, Vol.14 (3), p.321-336
Hauptverfasser:	Steinhäuser, Christine, Heigl, Ulrike, Tchikov, Vladimir, Schwarz, Jeanette, Gutsmann, Thomas, Seeger, Katrin, Brandenburg, Julius, Fritsch, Jürgen, Schroeder, Josef, Wiesmüller, Karl‐Heinz, Rosenkrands, Ida, Walther, Paul, Pott, Johanna, Krause, Eberhard, Ehlers, Stefan, Schneider‐Brachert, Wulf, Schütze, Stefan, Reiling, Norbert
Format:	Artikel
Sprache:	eng
Schlagworte:	Gram‐positive bacteria Humans infection Lipids - chemistry Listeria monocytogenes - isolation & purification macrophages Macrophages - microbiology Macrophages - ultrastructure magnetic isolation Magnets - chemistry Microscopy, Electron, Scanning Transmission Microscopy, Fluorescence mycobacteria Mycobacterium - isolation & purification phagosomes Phagosomes - microbiology Phagosomes - ultrastructure primary cells Staining and Labeling - methods tuberculosis
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