Inverse relationship between heat stable enterotoxin-b induced fluid accumulation and adherence of F4ac-positive enterotoxigenic Escherichia coli in ligated jejunal loops of F4ab/ac fimbria receptor-positive swine
Heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli (ETEC) increases bacterial adherence to porcine enterocytes in vitro and enhances small intestinal colonization in swine. Heat-stable enterotoxin-b (STb) is not known to affect colonization; however, through an induction of ne...
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| creator | Erume, Joseph Wijemanne, Prageeth Berberov, Emil M. Kachman, Stephen D. Oestmann, Daniel J. Francis, David H. Moxley, Rodney A. |
| description | Heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli (ETEC) increases bacterial adherence to porcine enterocytes in vitro and enhances small intestinal colonization in swine. Heat-stable enterotoxin-b (STb) is not known to affect colonization; however, through an induction of net fluid accumulation it might reduce bacterial adherence. The relationship between fluid accumulation and bacterial adherence in jejunal loops inoculated with ETEC strains that produce LT, STb, both, or neither toxin was studied. Ligated jejunal loops were constructed in weaned Yorkshire pigs in two independent experiments (Exp. 1, n=5, 8-week-old; Exp. 2, n=6, 6–8-week-old). Each pig was inoculated with six F4ac+E. coli strains: (1) LT+, STb+ parent (WAM2317); (2) STb− (ΔestB) mutant (MUN297); (3) MUN297 complemented with STb (MUN298); (4) LT− STb− (ΔeltAB ΔestB) mutant (MUN300); (5) MUN300 complemented with LT (MUN301); and (6) 1836-2 (non-enterotoxigenic, wild-type). Pigs were confirmed to be K88 (F4)ab/ac receptor-positive in Exp. 2 by testing for intestinal mucin-type glycoproteins and inferred to be receptor-positive in both Exp. 1 and 2 based on histopathologic evidence of bacterial adherence. Strains that produced STb induced marked fluid accumulation with the response (ml/cm) to WAM2317 and MUN298 significantly greater than that to the other strains (P |
| doi_str_mv | 10.1016/j.vetmic.2012.07.048 |
| format | Article |
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Heat-stable enterotoxin-b (STb) is not known to affect colonization; however, through an induction of net fluid accumulation it might reduce bacterial adherence. The relationship between fluid accumulation and bacterial adherence in jejunal loops inoculated with ETEC strains that produce LT, STb, both, or neither toxin was studied. Ligated jejunal loops were constructed in weaned Yorkshire pigs in two independent experiments (Exp. 1, n=5, 8-week-old; Exp. 2, n=6, 6–8-week-old). Each pig was inoculated with six F4ac+E. coli strains: (1) LT+, STb+ parent (WAM2317); (2) STb− (ΔestB) mutant (MUN297); (3) MUN297 complemented with STb (MUN298); (4) LT− STb− (ΔeltAB ΔestB) mutant (MUN300); (5) MUN300 complemented with LT (MUN301); and (6) 1836-2 (non-enterotoxigenic, wild-type). Pigs were confirmed to be K88 (F4)ab/ac receptor-positive in Exp. 2 by testing for intestinal mucin-type glycoproteins and inferred to be receptor-positive in both Exp. 1 and 2 based on histopathologic evidence of bacterial adherence. Strains that produced STb induced marked fluid accumulation with the response (ml/cm) to WAM2317 and MUN298 significantly greater than that to the other strains (P<0.0001). Conversely, bacterial adherence scores based on immunohistochemistry and CFU/g of washed mucosa were both lowest in the strains that expressed STb and highest in those that did not. For the two experiments combined, the Pearson correlation coefficient (R) between fluid volume (ml/cm) and log CFU per gram was −0.57021 (P<0.0001); R2=0.3521 (n=197). These results support the hypothesis that enterotoxin-induced fluid accumulation flushes progeny organisms into the lumen of the bowel, thereby increasing the likelihood of fecal shedding and transmission of the pathogen to new hosts.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2012.07.048</identifier><identifier>PMID: 22901529</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; bacterial adhesion ; Bacterial Adhesion - physiology ; Bacterial Toxins - biosynthesis ; Bacterial Toxins - metabolism ; correlation ; enterocytes ; Enterocytes - microbiology ; Enterocytes - pathology ; enterotoxigenic Escherichia coli ; Enterotoxigenic Escherichia coli - metabolism ; Enterotoxigenic Escherichia coli - physiology ; Enterotoxins - biosynthesis ; Enterotoxins - metabolism ; Escherichia coli ; Escherichia coli Infections - metabolism ; Escherichia coli Infections - microbiology ; Escherichia coli Infections - pathology ; Escherichia coli Infections - veterinary ; Escherichia coli Proteins - biosynthesis ; Escherichia coli Proteins - metabolism ; Fimbria ; Fimbriae, Bacterial - metabolism ; Fimbriae, Bacterial - pathology ; glycoproteins ; heat stability ; Heat-labile enterotoxin ; Heat-stable enterotoxin-b ; histopathology ; hosts ; immunohistochemistry ; Jejunum - microbiology ; Jejunum - pathology ; Microvilli - metabolism ; Microvilli - microbiology ; Microvilli - pathology ; Mucin-4 - genetics ; Mucin-4 - metabolism ; Mucin-4 gene ; mucosa ; mutants ; parents ; pathogens ; Polymorphism, Genetic ; progeny ; shedding ; Swine ; Swine Diseases - metabolism ; Swine Diseases - microbiology ; Swine Diseases - pathology ; toxins</subject><ispartof>Veterinary microbiology, 2013-01, Vol.161 (3-4), p.315-324</ispartof><rights>2012 Elsevier B.V.</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-36b969599232a442d312367a76bbd7d23a44f36bb89c9383517f5d39624269513</citedby><cites>FETCH-LOGICAL-c419t-36b969599232a442d312367a76bbd7d23a44f36bb89c9383517f5d39624269513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2012.07.048$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22901529$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Erume, Joseph</creatorcontrib><creatorcontrib>Wijemanne, Prageeth</creatorcontrib><creatorcontrib>Berberov, Emil M.</creatorcontrib><creatorcontrib>Kachman, Stephen D.</creatorcontrib><creatorcontrib>Oestmann, Daniel J.</creatorcontrib><creatorcontrib>Francis, David H.</creatorcontrib><creatorcontrib>Moxley, Rodney A.</creatorcontrib><title>Inverse relationship between heat stable enterotoxin-b induced fluid accumulation and adherence of F4ac-positive enterotoxigenic Escherichia coli in ligated jejunal loops of F4ab/ac fimbria receptor-positive swine</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli (ETEC) increases bacterial adherence to porcine enterocytes in vitro and enhances small intestinal colonization in swine. Heat-stable enterotoxin-b (STb) is not known to affect colonization; however, through an induction of net fluid accumulation it might reduce bacterial adherence. The relationship between fluid accumulation and bacterial adherence in jejunal loops inoculated with ETEC strains that produce LT, STb, both, or neither toxin was studied. Ligated jejunal loops were constructed in weaned Yorkshire pigs in two independent experiments (Exp. 1, n=5, 8-week-old; Exp. 2, n=6, 6–8-week-old). Each pig was inoculated with six F4ac+E. coli strains: (1) LT+, STb+ parent (WAM2317); (2) STb− (ΔestB) mutant (MUN297); (3) MUN297 complemented with STb (MUN298); (4) LT− STb− (ΔeltAB ΔestB) mutant (MUN300); (5) MUN300 complemented with LT (MUN301); and (6) 1836-2 (non-enterotoxigenic, wild-type). Pigs were confirmed to be K88 (F4)ab/ac receptor-positive in Exp. 2 by testing for intestinal mucin-type glycoproteins and inferred to be receptor-positive in both Exp. 1 and 2 based on histopathologic evidence of bacterial adherence. Strains that produced STb induced marked fluid accumulation with the response (ml/cm) to WAM2317 and MUN298 significantly greater than that to the other strains (P<0.0001). Conversely, bacterial adherence scores based on immunohistochemistry and CFU/g of washed mucosa were both lowest in the strains that expressed STb and highest in those that did not. For the two experiments combined, the Pearson correlation coefficient (R) between fluid volume (ml/cm) and log CFU per gram was −0.57021 (P<0.0001); R2=0.3521 (n=197). These results support the hypothesis that enterotoxin-induced fluid accumulation flushes progeny organisms into the lumen of the bowel, thereby increasing the likelihood of fecal shedding and transmission of the pathogen to new hosts.</description><subject>Animals</subject><subject>bacterial adhesion</subject><subject>Bacterial Adhesion - physiology</subject><subject>Bacterial Toxins - biosynthesis</subject><subject>Bacterial Toxins - metabolism</subject><subject>correlation</subject><subject>enterocytes</subject><subject>Enterocytes - microbiology</subject><subject>Enterocytes - pathology</subject><subject>enterotoxigenic Escherichia coli</subject><subject>Enterotoxigenic Escherichia coli - metabolism</subject><subject>Enterotoxigenic Escherichia coli - physiology</subject><subject>Enterotoxins - biosynthesis</subject><subject>Enterotoxins - metabolism</subject><subject>Escherichia coli</subject><subject>Escherichia coli Infections - metabolism</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Infections - pathology</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Escherichia coli Proteins - biosynthesis</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Fimbria</subject><subject>Fimbriae, Bacterial - metabolism</subject><subject>Fimbriae, Bacterial - pathology</subject><subject>glycoproteins</subject><subject>heat stability</subject><subject>Heat-labile enterotoxin</subject><subject>Heat-stable enterotoxin-b</subject><subject>histopathology</subject><subject>hosts</subject><subject>immunohistochemistry</subject><subject>Jejunum - microbiology</subject><subject>Jejunum - pathology</subject><subject>Microvilli - metabolism</subject><subject>Microvilli - microbiology</subject><subject>Microvilli - pathology</subject><subject>Mucin-4 - genetics</subject><subject>Mucin-4 - metabolism</subject><subject>Mucin-4 gene</subject><subject>mucosa</subject><subject>mutants</subject><subject>parents</subject><subject>pathogens</subject><subject>Polymorphism, Genetic</subject><subject>progeny</subject><subject>shedding</subject><subject>Swine</subject><subject>Swine Diseases - metabolism</subject><subject>Swine Diseases - microbiology</subject><subject>Swine Diseases - pathology</subject><subject>toxins</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu1DAUhiMEosPAGyDwkk1S33LxBglVLVSqxAK6thz7ZMajxA62My0PyvvgUYbLCrGydPT937HOXxSvCa4IJs3loTpCmqyuKCa0wm2Fefek2JCuZSWtOX1abDBru5IQVl8UL2I8YIy5aPDz4oJSgUlNxab4ceuOECKgAKNK1ru4tzPqIT0AOLQHlVBMqh8BgUsQfPKP1pU9ss4sGgwaxsUapLRepmUVIOXywOwhgNOA_IBuuNLl7KNN9vi3ZwfOanQddWat3luFtB9tVqPR7lTK9gMcFqdGNHo_x7Oqv1QaDXbqQw4E0DAnH_7o44N18LJ4Nqgxwqvzuy3ub66_Xn0q7z5_vL36cFdqTkQqWdOLRtRCUEYV59QwQlnTqrbpe9MayvJwyFDfCS1Yx2rSDrVhoqGc5hxh2-Ld6p2D_7ZATHKyUcM4Kgd-iZLQrs5lUVr_B0pJV7Ou4RnlK6qDjzHAIOdgJxW-S4LlqXt5kGv38tS9xK3M3efYm_OGpZ_A_A79KjsDb1dgUF6qXbBR3n_JBo5xXs3zFbbF-5WAfLSjhSCjtqcajc2XTtJ4--8__ATuuc7e</recordid><startdate>20130125</startdate><enddate>20130125</enddate><creator>Erume, Joseph</creator><creator>Wijemanne, Prageeth</creator><creator>Berberov, Emil M.</creator><creator>Kachman, Stephen D.</creator><creator>Oestmann, Daniel J.</creator><creator>Francis, David H.</creator><creator>Moxley, Rodney A.</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20130125</creationdate><title>Inverse relationship between heat stable enterotoxin-b induced fluid accumulation and adherence of F4ac-positive enterotoxigenic Escherichia coli in ligated jejunal loops of F4ab/ac fimbria receptor-positive swine</title><author>Erume, Joseph ; Wijemanne, Prageeth ; Berberov, Emil M. ; Kachman, Stephen D. ; Oestmann, Daniel J. ; Francis, David H. ; Moxley, Rodney A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-36b969599232a442d312367a76bbd7d23a44f36bb89c9383517f5d39624269513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>bacterial adhesion</topic><topic>Bacterial Adhesion - physiology</topic><topic>Bacterial Toxins - biosynthesis</topic><topic>Bacterial Toxins - metabolism</topic><topic>correlation</topic><topic>enterocytes</topic><topic>Enterocytes - microbiology</topic><topic>Enterocytes - pathology</topic><topic>enterotoxigenic Escherichia coli</topic><topic>Enterotoxigenic Escherichia coli - metabolism</topic><topic>Enterotoxigenic Escherichia coli - physiology</topic><topic>Enterotoxins - biosynthesis</topic><topic>Enterotoxins - metabolism</topic><topic>Escherichia coli</topic><topic>Escherichia coli Infections - metabolism</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Infections - pathology</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Escherichia coli Proteins - biosynthesis</topic><topic>Escherichia coli Proteins - metabolism</topic><topic>Fimbria</topic><topic>Fimbriae, Bacterial - metabolism</topic><topic>Fimbriae, Bacterial - pathology</topic><topic>glycoproteins</topic><topic>heat stability</topic><topic>Heat-labile enterotoxin</topic><topic>Heat-stable enterotoxin-b</topic><topic>histopathology</topic><topic>hosts</topic><topic>immunohistochemistry</topic><topic>Jejunum - microbiology</topic><topic>Jejunum - pathology</topic><topic>Microvilli - metabolism</topic><topic>Microvilli - microbiology</topic><topic>Microvilli - pathology</topic><topic>Mucin-4 - genetics</topic><topic>Mucin-4 - metabolism</topic><topic>Mucin-4 gene</topic><topic>mucosa</topic><topic>mutants</topic><topic>parents</topic><topic>pathogens</topic><topic>Polymorphism, Genetic</topic><topic>progeny</topic><topic>shedding</topic><topic>Swine</topic><topic>Swine Diseases - metabolism</topic><topic>Swine Diseases - microbiology</topic><topic>Swine Diseases - pathology</topic><topic>toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Erume, Joseph</creatorcontrib><creatorcontrib>Wijemanne, Prageeth</creatorcontrib><creatorcontrib>Berberov, Emil M.</creatorcontrib><creatorcontrib>Kachman, Stephen D.</creatorcontrib><creatorcontrib>Oestmann, Daniel J.</creatorcontrib><creatorcontrib>Francis, David H.</creatorcontrib><creatorcontrib>Moxley, Rodney A.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Erume, Joseph</au><au>Wijemanne, Prageeth</au><au>Berberov, Emil M.</au><au>Kachman, Stephen D.</au><au>Oestmann, Daniel J.</au><au>Francis, David H.</au><au>Moxley, Rodney A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inverse relationship between heat stable enterotoxin-b induced fluid accumulation and adherence of F4ac-positive enterotoxigenic Escherichia coli in ligated jejunal loops of F4ab/ac fimbria receptor-positive swine</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2013-01-25</date><risdate>2013</risdate><volume>161</volume><issue>3-4</issue><spage>315</spage><epage>324</epage><pages>315-324</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><abstract>Heat-labile enterotoxin (LT) produced by enterotoxigenic Escherichia coli (ETEC) increases bacterial adherence to porcine enterocytes in vitro and enhances small intestinal colonization in swine. Heat-stable enterotoxin-b (STb) is not known to affect colonization; however, through an induction of net fluid accumulation it might reduce bacterial adherence. The relationship between fluid accumulation and bacterial adherence in jejunal loops inoculated with ETEC strains that produce LT, STb, both, or neither toxin was studied. Ligated jejunal loops were constructed in weaned Yorkshire pigs in two independent experiments (Exp. 1, n=5, 8-week-old; Exp. 2, n=6, 6–8-week-old). Each pig was inoculated with six F4ac+E. coli strains: (1) LT+, STb+ parent (WAM2317); (2) STb− (ΔestB) mutant (MUN297); (3) MUN297 complemented with STb (MUN298); (4) LT− STb− (ΔeltAB ΔestB) mutant (MUN300); (5) MUN300 complemented with LT (MUN301); and (6) 1836-2 (non-enterotoxigenic, wild-type). Pigs were confirmed to be K88 (F4)ab/ac receptor-positive in Exp. 2 by testing for intestinal mucin-type glycoproteins and inferred to be receptor-positive in both Exp. 1 and 2 based on histopathologic evidence of bacterial adherence. Strains that produced STb induced marked fluid accumulation with the response (ml/cm) to WAM2317 and MUN298 significantly greater than that to the other strains (P<0.0001). Conversely, bacterial adherence scores based on immunohistochemistry and CFU/g of washed mucosa were both lowest in the strains that expressed STb and highest in those that did not. For the two experiments combined, the Pearson correlation coefficient (R) between fluid volume (ml/cm) and log CFU per gram was −0.57021 (P<0.0001); R2=0.3521 (n=197). These results support the hypothesis that enterotoxin-induced fluid accumulation flushes progeny organisms into the lumen of the bowel, thereby increasing the likelihood of fecal shedding and transmission of the pathogen to new hosts.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>22901529</pmid><doi>10.1016/j.vetmic.2012.07.048</doi><tpages>10</tpages></addata></record> |
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| ispartof | Veterinary microbiology, 2013-01, Vol.161 (3-4), p.315-324 |
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| subjects | Animals bacterial adhesion Bacterial Adhesion - physiology Bacterial Toxins - biosynthesis Bacterial Toxins - metabolism correlation enterocytes Enterocytes - microbiology Enterocytes - pathology enterotoxigenic Escherichia coli Enterotoxigenic Escherichia coli - metabolism Enterotoxigenic Escherichia coli - physiology Enterotoxins - biosynthesis Enterotoxins - metabolism Escherichia coli Escherichia coli Infections - metabolism Escherichia coli Infections - microbiology Escherichia coli Infections - pathology Escherichia coli Infections - veterinary Escherichia coli Proteins - biosynthesis Escherichia coli Proteins - metabolism Fimbria Fimbriae, Bacterial - metabolism Fimbriae, Bacterial - pathology glycoproteins heat stability Heat-labile enterotoxin Heat-stable enterotoxin-b histopathology hosts immunohistochemistry Jejunum - microbiology Jejunum - pathology Microvilli - metabolism Microvilli - microbiology Microvilli - pathology Mucin-4 - genetics Mucin-4 - metabolism Mucin-4 gene mucosa mutants parents pathogens Polymorphism, Genetic progeny shedding Swine Swine Diseases - metabolism Swine Diseases - microbiology Swine Diseases - pathology toxins |
| title | Inverse relationship between heat stable enterotoxin-b induced fluid accumulation and adherence of F4ac-positive enterotoxigenic Escherichia coli in ligated jejunal loops of F4ab/ac fimbria receptor-positive swine |
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