Emergence of VIM-4- and SHV-12-producing Enterobacter cloacae in a neonatal intensive care unit
Abstract In order to reveal colonization with multidrug-resistant bacteria early, routine screening is done on samples of all patients of the neonatal intensive care units at Semmelweis University, Hungary. Due to the extended-spectrum β-lactamase (ESBL) screening examinations, emergence of multidru...
Gespeichert in:
| Veröffentlicht in: | International journal of medical microbiology 2012-11, Vol.302 (6), p.257-260 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 260 |
|---|---|
| container_issue | 6 |
| container_start_page | 257 |
| container_title | International journal of medical microbiology |
| container_volume | 302 |
| creator | Juhász, Emese Jánvári, Laura Tóth, Ákos Damjanova, Ivelina Nobilis, András Kristóf, Katalin |
| description | Abstract In order to reveal colonization with multidrug-resistant bacteria early, routine screening is done on samples of all patients of the neonatal intensive care units at Semmelweis University, Hungary. Due to the extended-spectrum β-lactamase (ESBL) screening examinations, emergence of multidrug-resistant Enterobacter cloacae isolates was found with suspicion of clonal transmission, therefore active microbiological surveillance was initiated. The aim of our study was to characterize 60 E. cloacae isolates recovered in a 7-month period in 2010. MIC values of antibiotics were determined and ESBL and carbapenemase production was tested. Metallo-β-lactamase (MBL) genes, ESBL genes, and class-1 integrons were characterized, and the possible clonal relationship between isolates was investigated. The isolates showed increased MIC values for carbapenems and cephalosporins. All 60 E. cloacae strains recovered from 16 neonates proved to be VIM-4 MBL producers. Fifty-three strains were SHV-12 ESBL producers also. In all cases, the blaVIM-4 gene was a part of class-1 integron, In238a. XbaI-macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) revealed identical patterns for the isolates. Our study supports the importance of active microbiological surveillance as well as molecular epidemiology at the NICUs as a part of infection control. |
| doi_str_mv | 10.1016/j.ijmm.2012.05.003 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1285097983</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S1438422112000215</els_id><sourcerecordid>1285097983</sourcerecordid><originalsourceid>FETCH-LOGICAL-c488t-4650c6df6afaab775af14b0ca70ad6caf54d78c95fbe8fa0532c1b0cc37f83283</originalsourceid><addsrcrecordid>eNqFUsFu1TAQjBCIlsIPcEA-cnHwOonjSAgJVQ9aqYhDoVdr46wrh8QpdlKpf4-jVzhwgNPa2pnRamaK4jWIEgSod2Ppx3kupQBZiqYUonpSnIICzYUS7dP8rivNaynhpHiR0iiEkF2lnhcnUrYSNMBpYQ4zxVsKltji2M3lF15zhmFg1xc3HCS_i8uwWR9u2SGsFJcebR7MTgtaJOYDQxZoCbjilH8rheTviVmMxLbg15fFM4dToleP86z4_unw7fyCX339fHn-8YrbWuuV16oRVg1OoUPs27ZBB3UvLLYCB2XRNfXQats1riftUDSVtJD3tmqdrqSuzoq3R9188M-N0mpmnyxNE-brtmRA6kZ0baer_0MBatUpIVWGyiPUxiWlSM7cRT9jfDAgzJ6BGc2egdkzMKIxOYNMevOov_UzDX8ov03PgPdHAGVD7j1Fk6zfIxh8JLuaYfH_1v_wF91OPniL0w96oDQuWwzZagMmZY653luwlwDkXgBoql8sGqt1</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1114696026</pqid></control><display><type>article</type><title>Emergence of VIM-4- and SHV-12-producing Enterobacter cloacae in a neonatal intensive care unit</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Juhász, Emese ; Jánvári, Laura ; Tóth, Ákos ; Damjanova, Ivelina ; Nobilis, András ; Kristóf, Katalin</creator><creatorcontrib>Juhász, Emese ; Jánvári, Laura ; Tóth, Ákos ; Damjanova, Ivelina ; Nobilis, András ; Kristóf, Katalin</creatorcontrib><description>Abstract In order to reveal colonization with multidrug-resistant bacteria early, routine screening is done on samples of all patients of the neonatal intensive care units at Semmelweis University, Hungary. Due to the extended-spectrum β-lactamase (ESBL) screening examinations, emergence of multidrug-resistant Enterobacter cloacae isolates was found with suspicion of clonal transmission, therefore active microbiological surveillance was initiated. The aim of our study was to characterize 60 E. cloacae isolates recovered in a 7-month period in 2010. MIC values of antibiotics were determined and ESBL and carbapenemase production was tested. Metallo-β-lactamase (MBL) genes, ESBL genes, and class-1 integrons were characterized, and the possible clonal relationship between isolates was investigated. The isolates showed increased MIC values for carbapenems and cephalosporins. All 60 E. cloacae strains recovered from 16 neonates proved to be VIM-4 MBL producers. Fifty-three strains were SHV-12 ESBL producers also. In all cases, the blaVIM-4 gene was a part of class-1 integron, In238a. XbaI-macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) revealed identical patterns for the isolates. Our study supports the importance of active microbiological surveillance as well as molecular epidemiology at the NICUs as a part of infection control.</description><identifier>ISSN: 1438-4221</identifier><identifier>EISSN: 1618-0607</identifier><identifier>DOI: 10.1016/j.ijmm.2012.05.003</identifier><identifier>PMID: 22721811</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Antibiotics ; beta-Lactamases - genetics ; beta-Lactamases - metabolism ; Carbapenemase ; Carbapenems ; Carbapenems - pharmacology ; Ceftazidime - pharmacology ; Cephalosporins ; Colistin ; Colonization ; Deoxyribonucleases, Type II Site-Specific - metabolism ; Drug resistance ; Drug Resistance, Multiple, Bacterial ; Electrophoresis, Gel, Pulsed-Field - methods ; Enterobacter cloacae ; Enterobacter cloacae - drug effects ; Enterobacter cloacae - enzymology ; Enterobacter cloacae - genetics ; Enterobacter cloacae - isolation & purification ; Enterobacteriaceae Infections - epidemiology ; Enterobacteriaceae Infections - microbiology ; Environmental Microbiology ; Epidemiology ; ESBL ; Female ; Genes, Bacterial ; Gestational Age ; Humans ; Hungary - epidemiology ; Infant, Newborn ; Infection ; Infectious Disease ; Integrons ; Intensive care units ; Intensive Care Units, Neonatal ; Male ; Medical Education ; Microbial Sensitivity Tests ; Minimum inhibitory concentration ; Neonatal ; Neonates ; Pulsed-field gel electrophoresis ; VIM</subject><ispartof>International journal of medical microbiology, 2012-11, Vol.302 (6), p.257-260</ispartof><rights>Elsevier GmbH</rights><rights>2012 Elsevier GmbH</rights><rights>Copyright © 2012 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-4650c6df6afaab775af14b0ca70ad6caf54d78c95fbe8fa0532c1b0cc37f83283</citedby><cites>FETCH-LOGICAL-c488t-4650c6df6afaab775af14b0ca70ad6caf54d78c95fbe8fa0532c1b0cc37f83283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijmm.2012.05.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22721811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Juhász, Emese</creatorcontrib><creatorcontrib>Jánvári, Laura</creatorcontrib><creatorcontrib>Tóth, Ákos</creatorcontrib><creatorcontrib>Damjanova, Ivelina</creatorcontrib><creatorcontrib>Nobilis, András</creatorcontrib><creatorcontrib>Kristóf, Katalin</creatorcontrib><title>Emergence of VIM-4- and SHV-12-producing Enterobacter cloacae in a neonatal intensive care unit</title><title>International journal of medical microbiology</title><addtitle>Int J Med Microbiol</addtitle><description>Abstract In order to reveal colonization with multidrug-resistant bacteria early, routine screening is done on samples of all patients of the neonatal intensive care units at Semmelweis University, Hungary. Due to the extended-spectrum β-lactamase (ESBL) screening examinations, emergence of multidrug-resistant Enterobacter cloacae isolates was found with suspicion of clonal transmission, therefore active microbiological surveillance was initiated. The aim of our study was to characterize 60 E. cloacae isolates recovered in a 7-month period in 2010. MIC values of antibiotics were determined and ESBL and carbapenemase production was tested. Metallo-β-lactamase (MBL) genes, ESBL genes, and class-1 integrons were characterized, and the possible clonal relationship between isolates was investigated. The isolates showed increased MIC values for carbapenems and cephalosporins. All 60 E. cloacae strains recovered from 16 neonates proved to be VIM-4 MBL producers. Fifty-three strains were SHV-12 ESBL producers also. In all cases, the blaVIM-4 gene was a part of class-1 integron, In238a. XbaI-macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) revealed identical patterns for the isolates. Our study supports the importance of active microbiological surveillance as well as molecular epidemiology at the NICUs as a part of infection control.</description><subject>Antibiotics</subject><subject>beta-Lactamases - genetics</subject><subject>beta-Lactamases - metabolism</subject><subject>Carbapenemase</subject><subject>Carbapenems</subject><subject>Carbapenems - pharmacology</subject><subject>Ceftazidime - pharmacology</subject><subject>Cephalosporins</subject><subject>Colistin</subject><subject>Colonization</subject><subject>Deoxyribonucleases, Type II Site-Specific - metabolism</subject><subject>Drug resistance</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>Electrophoresis, Gel, Pulsed-Field - methods</subject><subject>Enterobacter cloacae</subject><subject>Enterobacter cloacae - drug effects</subject><subject>Enterobacter cloacae - enzymology</subject><subject>Enterobacter cloacae - genetics</subject><subject>Enterobacter cloacae - isolation & purification</subject><subject>Enterobacteriaceae Infections - epidemiology</subject><subject>Enterobacteriaceae Infections - microbiology</subject><subject>Environmental Microbiology</subject><subject>Epidemiology</subject><subject>ESBL</subject><subject>Female</subject><subject>Genes, Bacterial</subject><subject>Gestational Age</subject><subject>Humans</subject><subject>Hungary - epidemiology</subject><subject>Infant, Newborn</subject><subject>Infection</subject><subject>Infectious Disease</subject><subject>Integrons</subject><subject>Intensive care units</subject><subject>Intensive Care Units, Neonatal</subject><subject>Male</subject><subject>Medical Education</subject><subject>Microbial Sensitivity Tests</subject><subject>Minimum inhibitory concentration</subject><subject>Neonatal</subject><subject>Neonates</subject><subject>Pulsed-field gel electrophoresis</subject><subject>VIM</subject><issn>1438-4221</issn><issn>1618-0607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUsFu1TAQjBCIlsIPcEA-cnHwOonjSAgJVQ9aqYhDoVdr46wrh8QpdlKpf4-jVzhwgNPa2pnRamaK4jWIEgSod2Ppx3kupQBZiqYUonpSnIICzYUS7dP8rivNaynhpHiR0iiEkF2lnhcnUrYSNMBpYQ4zxVsKltji2M3lF15zhmFg1xc3HCS_i8uwWR9u2SGsFJcebR7MTgtaJOYDQxZoCbjilH8rheTviVmMxLbg15fFM4dToleP86z4_unw7fyCX339fHn-8YrbWuuV16oRVg1OoUPs27ZBB3UvLLYCB2XRNfXQats1riftUDSVtJD3tmqdrqSuzoq3R9188M-N0mpmnyxNE-brtmRA6kZ0baer_0MBatUpIVWGyiPUxiWlSM7cRT9jfDAgzJ6BGc2egdkzMKIxOYNMevOov_UzDX8ov03PgPdHAGVD7j1Fk6zfIxh8JLuaYfH_1v_wF91OPniL0w96oDQuWwzZagMmZY653luwlwDkXgBoql8sGqt1</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Juhász, Emese</creator><creator>Jánvári, Laura</creator><creator>Tóth, Ákos</creator><creator>Damjanova, Ivelina</creator><creator>Nobilis, András</creator><creator>Kristóf, Katalin</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20121101</creationdate><title>Emergence of VIM-4- and SHV-12-producing Enterobacter cloacae in a neonatal intensive care unit</title><author>Juhász, Emese ; Jánvári, Laura ; Tóth, Ákos ; Damjanova, Ivelina ; Nobilis, András ; Kristóf, Katalin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-4650c6df6afaab775af14b0ca70ad6caf54d78c95fbe8fa0532c1b0cc37f83283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Antibiotics</topic><topic>beta-Lactamases - genetics</topic><topic>beta-Lactamases - metabolism</topic><topic>Carbapenemase</topic><topic>Carbapenems</topic><topic>Carbapenems - pharmacology</topic><topic>Ceftazidime - pharmacology</topic><topic>Cephalosporins</topic><topic>Colistin</topic><topic>Colonization</topic><topic>Deoxyribonucleases, Type II Site-Specific - metabolism</topic><topic>Drug resistance</topic><topic>Drug Resistance, Multiple, Bacterial</topic><topic>Electrophoresis, Gel, Pulsed-Field - methods</topic><topic>Enterobacter cloacae</topic><topic>Enterobacter cloacae - drug effects</topic><topic>Enterobacter cloacae - enzymology</topic><topic>Enterobacter cloacae - genetics</topic><topic>Enterobacter cloacae - isolation & purification</topic><topic>Enterobacteriaceae Infections - epidemiology</topic><topic>Enterobacteriaceae Infections - microbiology</topic><topic>Environmental Microbiology</topic><topic>Epidemiology</topic><topic>ESBL</topic><topic>Female</topic><topic>Genes, Bacterial</topic><topic>Gestational Age</topic><topic>Humans</topic><topic>Hungary - epidemiology</topic><topic>Infant, Newborn</topic><topic>Infection</topic><topic>Infectious Disease</topic><topic>Integrons</topic><topic>Intensive care units</topic><topic>Intensive Care Units, Neonatal</topic><topic>Male</topic><topic>Medical Education</topic><topic>Microbial Sensitivity Tests</topic><topic>Minimum inhibitory concentration</topic><topic>Neonatal</topic><topic>Neonates</topic><topic>Pulsed-field gel electrophoresis</topic><topic>VIM</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Juhász, Emese</creatorcontrib><creatorcontrib>Jánvári, Laura</creatorcontrib><creatorcontrib>Tóth, Ákos</creatorcontrib><creatorcontrib>Damjanova, Ivelina</creatorcontrib><creatorcontrib>Nobilis, András</creatorcontrib><creatorcontrib>Kristóf, Katalin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>International journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Juhász, Emese</au><au>Jánvári, Laura</au><au>Tóth, Ákos</au><au>Damjanova, Ivelina</au><au>Nobilis, András</au><au>Kristóf, Katalin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Emergence of VIM-4- and SHV-12-producing Enterobacter cloacae in a neonatal intensive care unit</atitle><jtitle>International journal of medical microbiology</jtitle><addtitle>Int J Med Microbiol</addtitle><date>2012-11-01</date><risdate>2012</risdate><volume>302</volume><issue>6</issue><spage>257</spage><epage>260</epage><pages>257-260</pages><issn>1438-4221</issn><eissn>1618-0607</eissn><abstract>Abstract In order to reveal colonization with multidrug-resistant bacteria early, routine screening is done on samples of all patients of the neonatal intensive care units at Semmelweis University, Hungary. Due to the extended-spectrum β-lactamase (ESBL) screening examinations, emergence of multidrug-resistant Enterobacter cloacae isolates was found with suspicion of clonal transmission, therefore active microbiological surveillance was initiated. The aim of our study was to characterize 60 E. cloacae isolates recovered in a 7-month period in 2010. MIC values of antibiotics were determined and ESBL and carbapenemase production was tested. Metallo-β-lactamase (MBL) genes, ESBL genes, and class-1 integrons were characterized, and the possible clonal relationship between isolates was investigated. The isolates showed increased MIC values for carbapenems and cephalosporins. All 60 E. cloacae strains recovered from 16 neonates proved to be VIM-4 MBL producers. Fifty-three strains were SHV-12 ESBL producers also. In all cases, the blaVIM-4 gene was a part of class-1 integron, In238a. XbaI-macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) revealed identical patterns for the isolates. Our study supports the importance of active microbiological surveillance as well as molecular epidemiology at the NICUs as a part of infection control.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>22721811</pmid><doi>10.1016/j.ijmm.2012.05.003</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1438-4221 |
| ispartof | International journal of medical microbiology, 2012-11, Vol.302 (6), p.257-260 |
| issn | 1438-4221 1618-0607 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1285097983 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibiotics beta-Lactamases - genetics beta-Lactamases - metabolism Carbapenemase Carbapenems Carbapenems - pharmacology Ceftazidime - pharmacology Cephalosporins Colistin Colonization Deoxyribonucleases, Type II Site-Specific - metabolism Drug resistance Drug Resistance, Multiple, Bacterial Electrophoresis, Gel, Pulsed-Field - methods Enterobacter cloacae Enterobacter cloacae - drug effects Enterobacter cloacae - enzymology Enterobacter cloacae - genetics Enterobacter cloacae - isolation & purification Enterobacteriaceae Infections - epidemiology Enterobacteriaceae Infections - microbiology Environmental Microbiology Epidemiology ESBL Female Genes, Bacterial Gestational Age Humans Hungary - epidemiology Infant, Newborn Infection Infectious Disease Integrons Intensive care units Intensive Care Units, Neonatal Male Medical Education Microbial Sensitivity Tests Minimum inhibitory concentration Neonatal Neonates Pulsed-field gel electrophoresis VIM |
| title | Emergence of VIM-4- and SHV-12-producing Enterobacter cloacae in a neonatal intensive care unit |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T12%3A26%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Emergence%20of%20VIM-4-%20and%20SHV-12-producing%20Enterobacter%20cloacae%20in%20a%20neonatal%20intensive%20care%20unit&rft.jtitle=International%20journal%20of%20medical%20microbiology&rft.au=Juh%C3%A1sz,%20Emese&rft.date=2012-11-01&rft.volume=302&rft.issue=6&rft.spage=257&rft.epage=260&rft.pages=257-260&rft.issn=1438-4221&rft.eissn=1618-0607&rft_id=info:doi/10.1016/j.ijmm.2012.05.003&rft_dat=%3Cproquest_cross%3E1285097983%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1114696026&rft_id=info:pmid/22721811&rft_els_id=1_s2_0_S1438422112000215&rfr_iscdi=true |