Perinatal protein deprivation facilitates accumbal ERK phosphorylation in cocaine-sensitized adult rats
► Only D-animals exhibited behavioral sensitization to the lowest cocaine dose (5mg/kg). ► Pretreatment with cocaine 10mg/kg induced behavioral sensitization in C- and D-animals. ► In the NAc core only D-rats showed a significant pERK2 increase with the lowest dose of cocaine. ► C-rats with 10mg/kg...
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| creator | Velazquez, E.E. Valdomero, A. Maldonado, N.M. Orsingher, O.A. Cuadra, G.R. |
| description | ► Only D-animals exhibited behavioral sensitization to the lowest cocaine dose (5mg/kg). ► Pretreatment with cocaine 10mg/kg induced behavioral sensitization in C- and D-animals. ► In the NAc core only D-rats showed a significant pERK2 increase with the lowest dose of cocaine. ► C-rats with 10mg/kg showed an increase in pERK2 levels from WD 7 while D-rats showed this on WD 4. ► Undernutrition facilitates molecular processes involved in neuroplasticity during withdrawal.
In previous studies we described that perinatal protein deprivation facilitates the development and expression of behavioral sensitization to cocaine. In this research, we explored whether the increased reactivity observed in deprived (D) versus control (C) rats is also evident during drug-free withdrawal periods. Considering that activation of the extracellular signal-regulated protein kinase (ERK) is suggested to be involved in cocaine-induced behavioral sensitization, we study the effects of perinatal protein deprivation on phosphorylated ERK2 (pERK2) protein levels in the NAc (core and shell) during different drug-free withdrawal periods. To induce behavioral sensitization, C- and D-rats received a daily injection of cocaine (5–10mg/kg, i.p.) for 7 days and locomotor activity was performed on days 1 and 7. Cocaine-sensitized animals were left drug-free and pERK2 was assessed on withdrawal days (WD) 1, 4, 7 and 21. In the NAc core, cocaine induced ERK signaling pathway activation in a dose-dependent manner, and only D-rats showed a significant increase in pERK2 protein levels with the lowest dose of cocaine (5mg/kg). Moreover, sensitized C-rats with 10mg/kg showed an increase in pERK2 levels from WD7 while D-rats showed this activation on WD4, which remained increased on WD7 and 21. In contrast, in the NAc shell, only sensitized D-rats with cocaine 10mg/kg showed ERK2 activation on WD21. These results suggest that perinatal protein deprivation facilitates the molecular processes involved in neuronal plasticity occurring during withdrawal. |
| doi_str_mv | 10.1016/j.bbr.2012.12.020 |
| format | Article |
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In previous studies we described that perinatal protein deprivation facilitates the development and expression of behavioral sensitization to cocaine. In this research, we explored whether the increased reactivity observed in deprived (D) versus control (C) rats is also evident during drug-free withdrawal periods. Considering that activation of the extracellular signal-regulated protein kinase (ERK) is suggested to be involved in cocaine-induced behavioral sensitization, we study the effects of perinatal protein deprivation on phosphorylated ERK2 (pERK2) protein levels in the NAc (core and shell) during different drug-free withdrawal periods. To induce behavioral sensitization, C- and D-rats received a daily injection of cocaine (5–10mg/kg, i.p.) for 7 days and locomotor activity was performed on days 1 and 7. Cocaine-sensitized animals were left drug-free and pERK2 was assessed on withdrawal days (WD) 1, 4, 7 and 21. In the NAc core, cocaine induced ERK signaling pathway activation in a dose-dependent manner, and only D-rats showed a significant increase in pERK2 protein levels with the lowest dose of cocaine (5mg/kg). Moreover, sensitized C-rats with 10mg/kg showed an increase in pERK2 levels from WD7 while D-rats showed this activation on WD4, which remained increased on WD7 and 21. In contrast, in the NAc shell, only sensitized D-rats with cocaine 10mg/kg showed ERK2 activation on WD21. These results suggest that perinatal protein deprivation facilitates the molecular processes involved in neuronal plasticity occurring during withdrawal.</description><identifier>ISSN: 0166-4328</identifier><identifier>EISSN: 1872-7549</identifier><identifier>DOI: 10.1016/j.bbr.2012.12.020</identifier><identifier>PMID: 23266327</identifier><identifier>CODEN: BBREDI</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Animals ; Behavioral psychophysiology ; Behavioral sensitization ; Biological and medical sciences ; Cocaine ; Cocaine - pharmacology ; Extracellular signal-regulated kinase (ERK) ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Fundamental and applied biological sciences. Psychology ; Malnutrition - metabolism ; Medical sciences ; Metabolic diseases ; Motor Activity - drug effects ; Motor Activity - physiology ; Nucleus accumbens (NAc) ; Nucleus Accumbens - drug effects ; Nucleus Accumbens - metabolism ; Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...) ; Perinatal undernutrition ; Phosphorylation - drug effects ; Phosphorylation - physiology ; Psychology. Psychoanalysis. Psychiatry ; Psychology. Psychophysiology ; Rats ; Rats, Wistar ; Substance Withdrawal Syndrome - metabolism</subject><ispartof>Behavioural brain research, 2013-03, Vol.241, p.222-227</ispartof><rights>2012 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-68803067a509845d58f8c9a379625d47d3c2d6b46492556e361a99e309917bc53</citedby><cites>FETCH-LOGICAL-c416t-68803067a509845d58f8c9a379625d47d3c2d6b46492556e361a99e309917bc53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbr.2012.12.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27096301$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23266327$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Velazquez, E.E.</creatorcontrib><creatorcontrib>Valdomero, A.</creatorcontrib><creatorcontrib>Maldonado, N.M.</creatorcontrib><creatorcontrib>Orsingher, O.A.</creatorcontrib><creatorcontrib>Cuadra, G.R.</creatorcontrib><title>Perinatal protein deprivation facilitates accumbal ERK phosphorylation in cocaine-sensitized adult rats</title><title>Behavioural brain research</title><addtitle>Behav Brain Res</addtitle><description>► Only D-animals exhibited behavioral sensitization to the lowest cocaine dose (5mg/kg). ► Pretreatment with cocaine 10mg/kg induced behavioral sensitization in C- and D-animals. ► In the NAc core only D-rats showed a significant pERK2 increase with the lowest dose of cocaine. ► C-rats with 10mg/kg showed an increase in pERK2 levels from WD 7 while D-rats showed this on WD 4. ► Undernutrition facilitates molecular processes involved in neuroplasticity during withdrawal.
In previous studies we described that perinatal protein deprivation facilitates the development and expression of behavioral sensitization to cocaine. In this research, we explored whether the increased reactivity observed in deprived (D) versus control (C) rats is also evident during drug-free withdrawal periods. Considering that activation of the extracellular signal-regulated protein kinase (ERK) is suggested to be involved in cocaine-induced behavioral sensitization, we study the effects of perinatal protein deprivation on phosphorylated ERK2 (pERK2) protein levels in the NAc (core and shell) during different drug-free withdrawal periods. To induce behavioral sensitization, C- and D-rats received a daily injection of cocaine (5–10mg/kg, i.p.) for 7 days and locomotor activity was performed on days 1 and 7. Cocaine-sensitized animals were left drug-free and pERK2 was assessed on withdrawal days (WD) 1, 4, 7 and 21. In the NAc core, cocaine induced ERK signaling pathway activation in a dose-dependent manner, and only D-rats showed a significant increase in pERK2 protein levels with the lowest dose of cocaine (5mg/kg). Moreover, sensitized C-rats with 10mg/kg showed an increase in pERK2 levels from WD7 while D-rats showed this activation on WD4, which remained increased on WD7 and 21. In contrast, in the NAc shell, only sensitized D-rats with cocaine 10mg/kg showed ERK2 activation on WD21. These results suggest that perinatal protein deprivation facilitates the molecular processes involved in neuronal plasticity occurring during withdrawal.</description><subject>Animals</subject><subject>Behavioral psychophysiology</subject><subject>Behavioral sensitization</subject><subject>Biological and medical sciences</subject><subject>Cocaine</subject><subject>Cocaine - pharmacology</subject><subject>Extracellular signal-regulated kinase (ERK)</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Malnutrition - metabolism</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Motor Activity - drug effects</subject><subject>Motor Activity - physiology</subject><subject>Nucleus accumbens (NAc)</subject><subject>Nucleus Accumbens - drug effects</subject><subject>Nucleus Accumbens - metabolism</subject><subject>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</subject><subject>Perinatal undernutrition</subject><subject>Phosphorylation - drug effects</subject><subject>Phosphorylation - physiology</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychology. Psychophysiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Substance Withdrawal Syndrome - metabolism</subject><issn>0166-4328</issn><issn>1872-7549</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUuLFDEUhYMoTjv6A9xIbQQ31eb9wJUMoyMOKKLrcCu5pWmqq9okNTD-ejN0O-5EyCWb7xzuPYeQ54xuGWX69W47DHnLKePb9iinD8iGWcN7o6R7SDaN0b0U3J6RJ6XsKKWSKvaYnHHBtRbcbMj3z5jTDBWm7pCXimnuIh5yuoGalrkbIaQpVahYOghh3Q8NvPzysTv8WEqbfDsdwaYLS4A0Y19wLqmmXxg7iOtUuwy1PCWPRpgKPjv95-Tbu8uvF1f99af3Hy7eXvdBMl17bS0VVBtQ1FmporKjDQ6EcZqrKE0UgUc9SC0dV0qj0AycQ0GdY2YISpyTV0ffds3PFUv1-1QCThPMuKzFM26btZFO_gdqhLGMGtNQdkRDXkrJOPoW0R7yrWfU31Xhd75V4e-qaDrfqmiaFyf7ddhjvFf8yb4BL08AlADTmGEOqfzlDHVaUNa4N0cOW243CbMvIeEcMKaMofq4pH-s8RucfqXC</recordid><startdate>20130315</startdate><enddate>20130315</enddate><creator>Velazquez, E.E.</creator><creator>Valdomero, A.</creator><creator>Maldonado, N.M.</creator><creator>Orsingher, O.A.</creator><creator>Cuadra, G.R.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QG</scope><scope>7TK</scope></search><sort><creationdate>20130315</creationdate><title>Perinatal protein deprivation facilitates accumbal ERK phosphorylation in cocaine-sensitized adult rats</title><author>Velazquez, E.E. ; Valdomero, A. ; Maldonado, N.M. ; Orsingher, O.A. ; Cuadra, G.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-68803067a509845d58f8c9a379625d47d3c2d6b46492556e361a99e309917bc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Behavioral psychophysiology</topic><topic>Behavioral sensitization</topic><topic>Biological and medical sciences</topic><topic>Cocaine</topic><topic>Cocaine - pharmacology</topic><topic>Extracellular signal-regulated kinase (ERK)</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Malnutrition - metabolism</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Motor Activity - drug effects</topic><topic>Motor Activity - physiology</topic><topic>Nucleus accumbens (NAc)</topic><topic>Nucleus Accumbens - drug effects</topic><topic>Nucleus Accumbens - metabolism</topic><topic>Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...)</topic><topic>Perinatal undernutrition</topic><topic>Phosphorylation - drug effects</topic><topic>Phosphorylation - physiology</topic><topic>Psychology. Psychoanalysis. Psychiatry</topic><topic>Psychology. Psychophysiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Substance Withdrawal Syndrome - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Velazquez, E.E.</creatorcontrib><creatorcontrib>Valdomero, A.</creatorcontrib><creatorcontrib>Maldonado, N.M.</creatorcontrib><creatorcontrib>Orsingher, O.A.</creatorcontrib><creatorcontrib>Cuadra, G.R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Animal Behavior Abstracts</collection><collection>Neurosciences Abstracts</collection><jtitle>Behavioural brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Velazquez, E.E.</au><au>Valdomero, A.</au><au>Maldonado, N.M.</au><au>Orsingher, O.A.</au><au>Cuadra, G.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Perinatal protein deprivation facilitates accumbal ERK phosphorylation in cocaine-sensitized adult rats</atitle><jtitle>Behavioural brain research</jtitle><addtitle>Behav Brain Res</addtitle><date>2013-03-15</date><risdate>2013</risdate><volume>241</volume><spage>222</spage><epage>227</epage><pages>222-227</pages><issn>0166-4328</issn><eissn>1872-7549</eissn><coden>BBREDI</coden><abstract>► Only D-animals exhibited behavioral sensitization to the lowest cocaine dose (5mg/kg). ► Pretreatment with cocaine 10mg/kg induced behavioral sensitization in C- and D-animals. ► In the NAc core only D-rats showed a significant pERK2 increase with the lowest dose of cocaine. ► C-rats with 10mg/kg showed an increase in pERK2 levels from WD 7 while D-rats showed this on WD 4. ► Undernutrition facilitates molecular processes involved in neuroplasticity during withdrawal.
In previous studies we described that perinatal protein deprivation facilitates the development and expression of behavioral sensitization to cocaine. In this research, we explored whether the increased reactivity observed in deprived (D) versus control (C) rats is also evident during drug-free withdrawal periods. Considering that activation of the extracellular signal-regulated protein kinase (ERK) is suggested to be involved in cocaine-induced behavioral sensitization, we study the effects of perinatal protein deprivation on phosphorylated ERK2 (pERK2) protein levels in the NAc (core and shell) during different drug-free withdrawal periods. To induce behavioral sensitization, C- and D-rats received a daily injection of cocaine (5–10mg/kg, i.p.) for 7 days and locomotor activity was performed on days 1 and 7. Cocaine-sensitized animals were left drug-free and pERK2 was assessed on withdrawal days (WD) 1, 4, 7 and 21. In the NAc core, cocaine induced ERK signaling pathway activation in a dose-dependent manner, and only D-rats showed a significant increase in pERK2 protein levels with the lowest dose of cocaine (5mg/kg). Moreover, sensitized C-rats with 10mg/kg showed an increase in pERK2 levels from WD7 while D-rats showed this activation on WD4, which remained increased on WD7 and 21. In contrast, in the NAc shell, only sensitized D-rats with cocaine 10mg/kg showed ERK2 activation on WD21. These results suggest that perinatal protein deprivation facilitates the molecular processes involved in neuronal plasticity occurring during withdrawal.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>23266327</pmid><doi>10.1016/j.bbr.2012.12.020</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Behavioral psychophysiology Behavioral sensitization Biological and medical sciences Cocaine Cocaine - pharmacology Extracellular signal-regulated kinase (ERK) Extracellular Signal-Regulated MAP Kinases - metabolism Fundamental and applied biological sciences. Psychology Malnutrition - metabolism Medical sciences Metabolic diseases Motor Activity - drug effects Motor Activity - physiology Nucleus accumbens (NAc) Nucleus Accumbens - drug effects Nucleus Accumbens - metabolism Other nutritional diseases (malnutrition, nutritional and vitamin deficiencies...) Perinatal undernutrition Phosphorylation - drug effects Phosphorylation - physiology Psychology. Psychoanalysis. Psychiatry Psychology. Psychophysiology Rats Rats, Wistar Substance Withdrawal Syndrome - metabolism |
| title | Perinatal protein deprivation facilitates accumbal ERK phosphorylation in cocaine-sensitized adult rats |
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