In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina

In Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located i...

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Veröffentlicht in:Veterinary parasitology 2013-02, Vol.192 (1-3), p.296-300
Hauptverfasser: Cutullé, Christian, Lovis, Léonore, D’Agostino, Beatriz Inés, Balbiani, Gabriel Gerardo, Morici, Gabriel, Citroni, Daniel, Reggi, Julio, Caracostantogolo, Jorge Luis
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container_end_page 300
container_issue 1-3
container_start_page 296
container_title Veterinary parasitology
container_volume 192
creator Cutullé, Christian
Lovis, Léonore
D’Agostino, Beatriz Inés
Balbiani, Gabriel Gerardo
Morici, Gabriel
Citroni, Daniel
Reggi, Julio
Caracostantogolo, Jorge Luis
description In Argentina, the cattle tick Rhipicephalus microplus has already developed resistance to organophospates and synthetic pyrethroids. However, no cases of amitraz resistance have ever been recorded in this country despite its heavy use. A recent failure of amitraz to control ticks in a farm located in Santo Tomé, province of Corrientes, resulted in the collection of samples for acaricide resistance diagnosis. The modified Drummond adult immersion test (AIT) and the larval tarsal test (LTT) were performed separately in Argentina and Switzerland to evaluate efficacy of amitraz and other acaricides. The AIT showed that oviposition in the Santo Tomé field isolate was not inhibited when it was challenged to 250 and 500ppm amitraz, and 50ppm deltamethrin. However, oviposition was reduced by 90.6% when this field isolate was challenged to a combination of 400ppm ethion and 100ppm cypermethrin. To confirm the results obtained with the AIT, 2 additional tick samples were collected and shipped to Switzerland for resistance diagnosis of amitraz, cypermethrin and flumethrin, using the LTT. With this bioassay, the resistance ratios of the 2 field isolates were 32.5 and 57.0 for amitraz and between 5.9 and 27.2 for the synthetic pyrethroids. Both in vitro bioassays confirmed amitraz and synthetic pyrethroid resistance in the Santo Tomé samples. These results account for the first evidence of amitraz resistance in R. microplus in Argentina.
doi_str_mv 10.1016/j.vetpar.2012.10.014
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subjects Acaricides - pharmacology
Amitraz
Animals
Argentina
Cattle
Cattle Diseases - diagnosis
Cattle Diseases - parasitology
Cattle tick
Dose-Response Relationship, Drug
Drug Resistance
Female
Ixodidae
Larva
Oviposition
Parasite Egg Count - veterinary
Resistance
Rhipicephalus
Rhipicephalus - drug effects
Rhipicephalus - physiology
Rhipicephalus microplus
Tick Infestations - diagnosis
Tick Infestations - parasitology
Tick Infestations - veterinary
Toluidines - pharmacology
title In vitro diagnosis of the first case of amitraz resistance in Rhipicephalus microplus in Santo Tomé (Corrientes), Argentina
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