Effect of alkylphospholipids on Candida albicans biofilm formation and maturation
The aim of this study was to evaluate miltefosine and four synthetic compounds (TCAN26, TC19, TC106 and TC117) for their in vitro inhibitory activity against Candida albicans planktonic and biofilm cells and investigate whether these compounds are able to inhibit the biofilm formation and to reduce...
Gespeichert in:
| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2013-01, Vol.68 (1), p.113-125 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 125 |
|---|---|
| container_issue | 1 |
| container_start_page | 113 |
| container_title | Journal of antimicrobial chemotherapy |
| container_volume | 68 |
| creator | Vila, Taissa V M Ishida, Kelly de Souza, Wanderley Prousis, Kyriakos Calogeropoulou, Theodora Rozental, Sonia |
| description | The aim of this study was to evaluate miltefosine and four synthetic compounds (TCAN26, TC19, TC106 and TC117) for their in vitro inhibitory activity against Candida albicans planktonic and biofilm cells and investigate whether these compounds are able to inhibit the biofilm formation and to reduce the viability of mature C. albicans biofilm cells.
The XTT reduction assay and transmission and scanning electron microscopy were employed to determine the inhibitory effects of the test compounds in comparison with amphotericin B and fluconazole against both planktonic cells and sessile cells in biofilms.
C. albicans planktonic cells were susceptible to miltefosine, TCAN26 and TC19, all alkylphospholipid compounds. Miltefosine and TCAN26 present a fungicidal activity with similar values of MIC and minimum fungicidal concentration (MFC), ranging from 2 to 8 mg/L. Cell treatment with sub-inhibitory concentrations of alkylphospholipids induced several ultrastructural alterations. In relation to biofilms, miltefosine reduced formation (38%-71%) and mature biofilms viability (32%-44%), at concentrations of 64 mg/L. TCAN26 also reduced biofilm formation (24%-30%) and mature biofilm viability (15%-20%), at concentrations of 64 mg/L. Although amphotericin B reduced biofilm formation similarly to miltefosine (51%-74%), its activity was lower on mature biofilms (24%-30%). Miltefosine antibiofilm activity was significantly higher than amphotericin B, on both formation and mature biofilms (P |
| doi_str_mv | 10.1093/jac/dks353 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1285091953</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2845256481</sourcerecordid><originalsourceid>FETCH-LOGICAL-c348t-217021fc2c03252197af2a74b88edb7d5de25ae009f37c6d61e100580c3abd23</originalsourceid><addsrcrecordid>eNqN0U1LwzAYB_AgipvTix9ACl5EqHuSNG1zlDFfYCDC7iXNC2Zrm5q0B7-92YsePHkISciPP3n4I3SN4QEDp_ONkHO1DZTREzTFWQ4pAY5P0RQosLTIGJ2gixA2AJCzvDxHE0I4Z8CLKXpfGqPlkDiTiGb71fQfLsTV2N6qkLguWYhOWSXia22l6EJSW2ds0ybG-VYMNpIokngc_f56ic6MaIK-Ou4ztH5arhcv6ert-XXxuEolzcohJbgAgo0kEihhBPNCGCKKrC5LrepCMaUJExqAG1rIXOVYYwBWgqSiVoTO0N0htvfuc9RhqFobpG4a0Wk3hgqTMk6IOaP_oFn8Ss7wLvX2D9240Xdxjr2iLCcUR3V_UNK7ELw2Ve9tK_xXhaHaVVLFSqpDJRHfHCPHutXql_50QL8B2yyGtg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1240356231</pqid></control><display><type>article</type><title>Effect of alkylphospholipids on Candida albicans biofilm formation and maturation</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Vila, Taissa V M ; Ishida, Kelly ; de Souza, Wanderley ; Prousis, Kyriakos ; Calogeropoulou, Theodora ; Rozental, Sonia</creator><creatorcontrib>Vila, Taissa V M ; Ishida, Kelly ; de Souza, Wanderley ; Prousis, Kyriakos ; Calogeropoulou, Theodora ; Rozental, Sonia</creatorcontrib><description>The aim of this study was to evaluate miltefosine and four synthetic compounds (TCAN26, TC19, TC106 and TC117) for their in vitro inhibitory activity against Candida albicans planktonic and biofilm cells and investigate whether these compounds are able to inhibit the biofilm formation and to reduce the viability of mature C. albicans biofilm cells.
The XTT reduction assay and transmission and scanning electron microscopy were employed to determine the inhibitory effects of the test compounds in comparison with amphotericin B and fluconazole against both planktonic cells and sessile cells in biofilms.
C. albicans planktonic cells were susceptible to miltefosine, TCAN26 and TC19, all alkylphospholipid compounds. Miltefosine and TCAN26 present a fungicidal activity with similar values of MIC and minimum fungicidal concentration (MFC), ranging from 2 to 8 mg/L. Cell treatment with sub-inhibitory concentrations of alkylphospholipids induced several ultrastructural alterations. In relation to biofilms, miltefosine reduced formation (38%-71%) and mature biofilms viability (32%-44%), at concentrations of 64 mg/L. TCAN26 also reduced biofilm formation (24%-30%) and mature biofilm viability (15%-20%), at concentrations of 64 mg/L. Although amphotericin B reduced biofilm formation similarly to miltefosine (51%-74%), its activity was lower on mature biofilms (24%-30%). Miltefosine antibiofilm activity was significantly higher than amphotericin B, on both formation and mature biofilms (P<0.05 and P<0.0001, respectively). Fluconazole was the least effective compound tested.
Promising antibiofilm activity was displayed by miltefosine and other alkylphosphocholine compounds, which could be considered a putative option for future treatment of candidaemia associated with biofilm formation, although further evaluation in in vivo systems is required.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dks353</identifier><identifier>PMID: 22995097</identifier><language>eng</language><publisher>England: Oxford Publishing Limited (England)</publisher><subject>Antifungal Agents - pharmacology ; Bacteria ; Biofilms ; Biofilms - drug effects ; Biofilms - growth & development ; Candida albicans ; Candida albicans - drug effects ; Candida albicans - growth & development ; Cells ; Fungi ; Lipids ; Phospholipids - pharmacology ; Phosphorylcholine - analogs & derivatives ; Phosphorylcholine - pharmacology ; Plankton ; Plankton - drug effects ; Plankton - microbiology ; Scanning electron microscopy ; Treatment Outcome</subject><ispartof>Journal of antimicrobial chemotherapy, 2013-01, Vol.68 (1), p.113-125</ispartof><rights>Copyright Oxford Publishing Limited(England) Jan 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-217021fc2c03252197af2a74b88edb7d5de25ae009f37c6d61e100580c3abd23</citedby><cites>FETCH-LOGICAL-c348t-217021fc2c03252197af2a74b88edb7d5de25ae009f37c6d61e100580c3abd23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22995097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vila, Taissa V M</creatorcontrib><creatorcontrib>Ishida, Kelly</creatorcontrib><creatorcontrib>de Souza, Wanderley</creatorcontrib><creatorcontrib>Prousis, Kyriakos</creatorcontrib><creatorcontrib>Calogeropoulou, Theodora</creatorcontrib><creatorcontrib>Rozental, Sonia</creatorcontrib><title>Effect of alkylphospholipids on Candida albicans biofilm formation and maturation</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>The aim of this study was to evaluate miltefosine and four synthetic compounds (TCAN26, TC19, TC106 and TC117) for their in vitro inhibitory activity against Candida albicans planktonic and biofilm cells and investigate whether these compounds are able to inhibit the biofilm formation and to reduce the viability of mature C. albicans biofilm cells.
The XTT reduction assay and transmission and scanning electron microscopy were employed to determine the inhibitory effects of the test compounds in comparison with amphotericin B and fluconazole against both planktonic cells and sessile cells in biofilms.
C. albicans planktonic cells were susceptible to miltefosine, TCAN26 and TC19, all alkylphospholipid compounds. Miltefosine and TCAN26 present a fungicidal activity with similar values of MIC and minimum fungicidal concentration (MFC), ranging from 2 to 8 mg/L. Cell treatment with sub-inhibitory concentrations of alkylphospholipids induced several ultrastructural alterations. In relation to biofilms, miltefosine reduced formation (38%-71%) and mature biofilms viability (32%-44%), at concentrations of 64 mg/L. TCAN26 also reduced biofilm formation (24%-30%) and mature biofilm viability (15%-20%), at concentrations of 64 mg/L. Although amphotericin B reduced biofilm formation similarly to miltefosine (51%-74%), its activity was lower on mature biofilms (24%-30%). Miltefosine antibiofilm activity was significantly higher than amphotericin B, on both formation and mature biofilms (P<0.05 and P<0.0001, respectively). Fluconazole was the least effective compound tested.
Promising antibiofilm activity was displayed by miltefosine and other alkylphosphocholine compounds, which could be considered a putative option for future treatment of candidaemia associated with biofilm formation, although further evaluation in in vivo systems is required.</description><subject>Antifungal Agents - pharmacology</subject><subject>Bacteria</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Candida albicans</subject><subject>Candida albicans - drug effects</subject><subject>Candida albicans - growth & development</subject><subject>Cells</subject><subject>Fungi</subject><subject>Lipids</subject><subject>Phospholipids - pharmacology</subject><subject>Phosphorylcholine - analogs & derivatives</subject><subject>Phosphorylcholine - pharmacology</subject><subject>Plankton</subject><subject>Plankton - drug effects</subject><subject>Plankton - microbiology</subject><subject>Scanning electron microscopy</subject><subject>Treatment Outcome</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U1LwzAYB_AgipvTix9ACl5EqHuSNG1zlDFfYCDC7iXNC2Zrm5q0B7-92YsePHkISciPP3n4I3SN4QEDp_ONkHO1DZTREzTFWQ4pAY5P0RQosLTIGJ2gixA2AJCzvDxHE0I4Z8CLKXpfGqPlkDiTiGb71fQfLsTV2N6qkLguWYhOWSXia22l6EJSW2ds0ybG-VYMNpIokngc_f56ic6MaIK-Ou4ztH5arhcv6ert-XXxuEolzcohJbgAgo0kEihhBPNCGCKKrC5LrepCMaUJExqAG1rIXOVYYwBWgqSiVoTO0N0htvfuc9RhqFobpG4a0Wk3hgqTMk6IOaP_oFn8Ss7wLvX2D9240Xdxjr2iLCcUR3V_UNK7ELw2Ve9tK_xXhaHaVVLFSqpDJRHfHCPHutXql_50QL8B2yyGtg</recordid><startdate>201301</startdate><enddate>201301</enddate><creator>Vila, Taissa V M</creator><creator>Ishida, Kelly</creator><creator>de Souza, Wanderley</creator><creator>Prousis, Kyriakos</creator><creator>Calogeropoulou, Theodora</creator><creator>Rozental, Sonia</creator><general>Oxford Publishing Limited (England)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>201301</creationdate><title>Effect of alkylphospholipids on Candida albicans biofilm formation and maturation</title><author>Vila, Taissa V M ; Ishida, Kelly ; de Souza, Wanderley ; Prousis, Kyriakos ; Calogeropoulou, Theodora ; Rozental, Sonia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-217021fc2c03252197af2a74b88edb7d5de25ae009f37c6d61e100580c3abd23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antifungal Agents - pharmacology</topic><topic>Bacteria</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth & development</topic><topic>Candida albicans</topic><topic>Candida albicans - drug effects</topic><topic>Candida albicans - growth & development</topic><topic>Cells</topic><topic>Fungi</topic><topic>Lipids</topic><topic>Phospholipids - pharmacology</topic><topic>Phosphorylcholine - analogs & derivatives</topic><topic>Phosphorylcholine - pharmacology</topic><topic>Plankton</topic><topic>Plankton - drug effects</topic><topic>Plankton - microbiology</topic><topic>Scanning electron microscopy</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vila, Taissa V M</creatorcontrib><creatorcontrib>Ishida, Kelly</creatorcontrib><creatorcontrib>de Souza, Wanderley</creatorcontrib><creatorcontrib>Prousis, Kyriakos</creatorcontrib><creatorcontrib>Calogeropoulou, Theodora</creatorcontrib><creatorcontrib>Rozental, Sonia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vila, Taissa V M</au><au>Ishida, Kelly</au><au>de Souza, Wanderley</au><au>Prousis, Kyriakos</au><au>Calogeropoulou, Theodora</au><au>Rozental, Sonia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of alkylphospholipids on Candida albicans biofilm formation and maturation</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2013-01</date><risdate>2013</risdate><volume>68</volume><issue>1</issue><spage>113</spage><epage>125</epage><pages>113-125</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><abstract>The aim of this study was to evaluate miltefosine and four synthetic compounds (TCAN26, TC19, TC106 and TC117) for their in vitro inhibitory activity against Candida albicans planktonic and biofilm cells and investigate whether these compounds are able to inhibit the biofilm formation and to reduce the viability of mature C. albicans biofilm cells.
The XTT reduction assay and transmission and scanning electron microscopy were employed to determine the inhibitory effects of the test compounds in comparison with amphotericin B and fluconazole against both planktonic cells and sessile cells in biofilms.
C. albicans planktonic cells were susceptible to miltefosine, TCAN26 and TC19, all alkylphospholipid compounds. Miltefosine and TCAN26 present a fungicidal activity with similar values of MIC and minimum fungicidal concentration (MFC), ranging from 2 to 8 mg/L. Cell treatment with sub-inhibitory concentrations of alkylphospholipids induced several ultrastructural alterations. In relation to biofilms, miltefosine reduced formation (38%-71%) and mature biofilms viability (32%-44%), at concentrations of 64 mg/L. TCAN26 also reduced biofilm formation (24%-30%) and mature biofilm viability (15%-20%), at concentrations of 64 mg/L. Although amphotericin B reduced biofilm formation similarly to miltefosine (51%-74%), its activity was lower on mature biofilms (24%-30%). Miltefosine antibiofilm activity was significantly higher than amphotericin B, on both formation and mature biofilms (P<0.05 and P<0.0001, respectively). Fluconazole was the least effective compound tested.
Promising antibiofilm activity was displayed by miltefosine and other alkylphosphocholine compounds, which could be considered a putative option for future treatment of candidaemia associated with biofilm formation, although further evaluation in in vivo systems is required.</abstract><cop>England</cop><pub>Oxford Publishing Limited (England)</pub><pmid>22995097</pmid><doi>10.1093/jac/dks353</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-7453 |
| ispartof | Journal of antimicrobial chemotherapy, 2013-01, Vol.68 (1), p.113-125 |
| issn | 0305-7453 1460-2091 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1285091953 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Antifungal Agents - pharmacology Bacteria Biofilms Biofilms - drug effects Biofilms - growth & development Candida albicans Candida albicans - drug effects Candida albicans - growth & development Cells Fungi Lipids Phospholipids - pharmacology Phosphorylcholine - analogs & derivatives Phosphorylcholine - pharmacology Plankton Plankton - drug effects Plankton - microbiology Scanning electron microscopy Treatment Outcome |
| title | Effect of alkylphospholipids on Candida albicans biofilm formation and maturation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T13%3A26%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20alkylphospholipids%20on%20Candida%20albicans%20biofilm%20formation%20and%20maturation&rft.jtitle=Journal%20of%20antimicrobial%20chemotherapy&rft.au=Vila,%20Taissa%20V%20M&rft.date=2013-01&rft.volume=68&rft.issue=1&rft.spage=113&rft.epage=125&rft.pages=113-125&rft.issn=0305-7453&rft.eissn=1460-2091&rft_id=info:doi/10.1093/jac/dks353&rft_dat=%3Cproquest_cross%3E2845256481%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1240356231&rft_id=info:pmid/22995097&rfr_iscdi=true |