Cloning and Expression of Brain-Specific Anion Transporter BSAT1 and Isolation of Monoclonal Antibodies to Its Extracellular Fragment

Brain-specifi c anion transporter BSAT1 extracellular fragment (451-557) cDNA was cloned in a vector for prokaryotic expression, a producer E. coli strain was obtained, and recombinant extracellular fragment BSAT1 451-557 was purifi ed and used for immunization of BALB/c mice. Splenic cells from mic...

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Veröffentlicht in:	Bulletin of experimental biology and medicine 2012-04, Vol.152 (6), p.734-738
Hauptverfasser:	Baklaushev, V. P., Kardashova, K. Sh, Gurina, O. I., Leopol’d, A. V., Semenova, A. V., Ryabukhin, I. A., Chekhonin, V. P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Anions Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Biological products Biomedical and Life Sciences Biomedicine Blood-brain barrier Brain Brain - metabolism Cell Biology Cloning Cloning, Molecular Drug development Enzyme-linked immunosorbent assay Escherichia coli Escherichia coli - genetics Expression vectors Gene Expression Genetic Vectors Humans Hybridoma Hybridomas - immunology Hybridomas - metabolism Immune response Immunization Immunoassay Immunoblotting Immunology and Microbiology Internal Medicine Laboratory Medicine Mice Molecular Sequence Data Monoclonal antibodies Organic Anion Transporters - chemistry Organic Anion Transporters - genetics Organic Anion Transporters - metabolism Pathology Protein Binding Protein Structure, Tertiary Rats Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Sensitivity and Specificity Spleen
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