Picking up the Pieces: A Generic Porous Si Biosensor for Probing the Proteolytic Products of Enzymes

A multifunctional porous Si biosensor that can both monitor the enzymatic activity of minute samples and allow subsequent retrieval of the entrapped proteolytic products for mass spectrometry analysis is described. The biosensor is constructed by DNA-directed/reversible immobilization of enzymes ont...

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Veröffentlicht in:	Analytical chemistry (Washington) 2013-02, Vol.85 (3), p.1951-1956
Hauptverfasser:	Shtenberg, Giorgi, Massad-Ivanir, Naama, Moscovitz, Oren, Engin, Sinem, Sharon, Michal, Fruk, Ljiljana, Segal, Ester
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Biosensors Cattle Enzymes Enzymes, Immobilized - analysis Enzymes, Immobilized - genetics Hybridization Mass spectrometry Molecular Sequence Data Porosity Protein Structure, Secondary Proteolysis Proteomics Silicon Dioxide - chemistry Surface Properties
Online-Zugang:	Volltext
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