Results of pap smears and immunocytochemical detection of the p16 and Ki67 proteins in women with cervical intraepithelial neoplasia and cervical cancer
The aim of the study was to assess the diagnostic value of pap smears and detection of the p16 and Ki67 proteins in women with cervical intraepithelial neoplasia (CIN). 630 women, aged between 25 and 65, with abnormal pap smears were included into the study All patients had a control pap smear and i...
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| Veröffentlicht in: | Ginekologia polska 2012-11, Vol.83 (11), p.822-826 |
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| creator | Rokita, Wojciech Skawiński, Dariusz Zmelonek-Znamirowska, Anna Kedzia, Witold Karowicz-Bilińska, Agata Spaczyński, Robert Spaczyński, Marek |
| description | The aim of the study was to assess the diagnostic value of pap smears and detection of the p16 and Ki67 proteins in women with cervical intraepithelial neoplasia (CIN).
630 women, aged between 25 and 65, with abnormal pap smears were included into the study All patients had a control pap smear and in each case punch biopsy with endocervical curettage were performed under the control of a colposcope. The presence of p16 and Ki67 proteins was detected using the CINtecPlus test. The results of the research were statistically assessed.
Abnormal pap smears were found in 82.5% (520/630) of the studied women. In 40% (252/630) of the cases the LSIL changes were found. The recognition of ASC-US concerned 35.2% (222/630) of the patients, and pap smears with the HSIL result were found in 7.3% (46/630) of the women. In 17.5% (110/630) of the patients the result of the cytological examination was normal. Abnormal results of the pap smears were found significantly statistically more frequently (p |
| format | Article |
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630 women, aged between 25 and 65, with abnormal pap smears were included into the study All patients had a control pap smear and in each case punch biopsy with endocervical curettage were performed under the control of a colposcope. The presence of p16 and Ki67 proteins was detected using the CINtecPlus test. The results of the research were statistically assessed.
Abnormal pap smears were found in 82.5% (520/630) of the studied women. In 40% (252/630) of the cases the LSIL changes were found. The recognition of ASC-US concerned 35.2% (222/630) of the patients, and pap smears with the HSIL result were found in 7.3% (46/630) of the women. In 17.5% (110/630) of the patients the result of the cytological examination was normal. Abnormal results of the pap smears were found significantly statistically more frequently (p<0,0001) in women with cervical intraepithelial neoplasia (CIN). The results of the CINtecPlus test were positive in 68,4% of women with CIN and in 33,3% of patients with normal cervix. In the group of women with precancerous lesions (HGSIL/CIN2+) the diagnostic accuracy of the pap smear was 41% for the cytological results ASC-US, 56% LSIL and 73% for detection of HSIL. Immunocytochemical detection of p16 and Ki67 proteins gained the highest accuracy (78%) in recognition of cervical precancerous lesions.
1. ASC-US and LSIL cytological recognition has low accuracy in the diagnosis of CIN2+ cervical changes. 2. Cytological recognition of HSIL has the highest accuracy in the diagnosis of CIN2+ changes. 3. Immunocytochemical detection of p16 and Ki67 proteins is more accurate in recognizing precancerous states and cervical cancer than cytological examination. 4. Immunocytochemical detection of the p16 and Ki67 proteins can be used to triage patients with atypical squamous cells of undetermined significance and low grade squamous intraepithelial lesions.</description><identifier>ISSN: 0017-0011</identifier><identifier>EISSN: 2543-6767</identifier><identifier>PMID: 23379189</identifier><language>pol</language><publisher>Poland: Wydawnictwo Via Medica</publisher><subject>Adult ; Aged ; Biomarkers, Tumor - analysis ; Cervical cancer ; Cervical Intraepithelial Neoplasia - metabolism ; Cervical Intraepithelial Neoplasia - pathology ; Cervix Uteri - chemistry ; Cervix Uteri - metabolism ; Cervix Uteri - pathology ; Cyclin-Dependent Kinase Inhibitor p16 ; Early Detection of Cancer - methods ; Female ; Humans ; Immunohistochemistry ; Ki-67 Antigen - analysis ; Male ; Middle Aged ; Neoplasm Proteins - analysis ; Pap smear ; Papanicolaou Test ; Poland ; Predictive Value of Tests ; Proteins ; Reproducibility of Results ; Uterine Cervical Neoplasms - chemistry ; Uterine Cervical Neoplasms - pathology ; Vagina - pathology ; Vaginal Smears</subject><ispartof>Ginekologia polska, 2012-11, Vol.83 (11), p.822-826</ispartof><rights>2012. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23379189$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rokita, Wojciech</creatorcontrib><creatorcontrib>Skawiński, Dariusz</creatorcontrib><creatorcontrib>Zmelonek-Znamirowska, Anna</creatorcontrib><creatorcontrib>Kedzia, Witold</creatorcontrib><creatorcontrib>Karowicz-Bilińska, Agata</creatorcontrib><creatorcontrib>Spaczyński, Robert</creatorcontrib><creatorcontrib>Spaczyński, Marek</creatorcontrib><title>Results of pap smears and immunocytochemical detection of the p16 and Ki67 proteins in women with cervical intraepithelial neoplasia and cervical cancer</title><title>Ginekologia polska</title><addtitle>Ginekol Pol</addtitle><description>The aim of the study was to assess the diagnostic value of pap smears and detection of the p16 and Ki67 proteins in women with cervical intraepithelial neoplasia (CIN).
630 women, aged between 25 and 65, with abnormal pap smears were included into the study All patients had a control pap smear and in each case punch biopsy with endocervical curettage were performed under the control of a colposcope. The presence of p16 and Ki67 proteins was detected using the CINtecPlus test. The results of the research were statistically assessed.
Abnormal pap smears were found in 82.5% (520/630) of the studied women. In 40% (252/630) of the cases the LSIL changes were found. The recognition of ASC-US concerned 35.2% (222/630) of the patients, and pap smears with the HSIL result were found in 7.3% (46/630) of the women. In 17.5% (110/630) of the patients the result of the cytological examination was normal. Abnormal results of the pap smears were found significantly statistically more frequently (p<0,0001) in women with cervical intraepithelial neoplasia (CIN). The results of the CINtecPlus test were positive in 68,4% of women with CIN and in 33,3% of patients with normal cervix. In the group of women with precancerous lesions (HGSIL/CIN2+) the diagnostic accuracy of the pap smear was 41% for the cytological results ASC-US, 56% LSIL and 73% for detection of HSIL. Immunocytochemical detection of p16 and Ki67 proteins gained the highest accuracy (78%) in recognition of cervical precancerous lesions.
1. ASC-US and LSIL cytological recognition has low accuracy in the diagnosis of CIN2+ cervical changes. 2. Cytological recognition of HSIL has the highest accuracy in the diagnosis of CIN2+ changes. 3. Immunocytochemical detection of p16 and Ki67 proteins is more accurate in recognizing precancerous states and cervical cancer than cytological examination. 4. Immunocytochemical detection of the p16 and Ki67 proteins can be used to triage patients with atypical squamous cells of undetermined significance and low grade squamous intraepithelial lesions.</description><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Cervical cancer</subject><subject>Cervical Intraepithelial Neoplasia - metabolism</subject><subject>Cervical Intraepithelial Neoplasia - pathology</subject><subject>Cervix Uteri - chemistry</subject><subject>Cervix Uteri - metabolism</subject><subject>Cervix Uteri - pathology</subject><subject>Cyclin-Dependent Kinase Inhibitor p16</subject><subject>Early Detection of Cancer - methods</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Ki-67 Antigen - analysis</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm Proteins - analysis</subject><subject>Pap smear</subject><subject>Papanicolaou Test</subject><subject>Poland</subject><subject>Predictive Value of Tests</subject><subject>Proteins</subject><subject>Reproducibility of Results</subject><subject>Uterine Cervical Neoplasms - chemistry</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Vagina - pathology</subject><subject>Vaginal Smears</subject><issn>0017-0011</issn><issn>2543-6767</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkMtKxDAUhoMoOozzChJw46bQ5LRJsxTxhgOC6Lpk0lMmQ5PWJlXmTXxcM-PowrM4N778-TlHZMbLAjIhhTwmszxnMkuJnZFFCJs8heCSK3VKzjiAVKxSM_L1gmHqYqB9Swc90OBQj4Fq31Dr3OR7s429WaOzRne0wYgm2t7v8LhGOjCxZ5-skHQY-4jWB2o9_ewdpmzjmhocP_avrY-jxiHtsLNp9tgPnQ5W7yX-MKN96s_JSau7gItDnZO3u9vXm4ds-Xz_eHO9zNLXKmaqqcq2NUZXFUiABiSixtWKt5iXJofUlI1M26qRBRcMcsC21EyBVi2Cgjm5-tFN7t8nDLF2NhjsOp3sTaFmvCoEZ8DKhF7-Qzf9NPrkruaFKDhjIHaCFwdqWjls6mG0To_b-vfm8A2m-IFN</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Rokita, Wojciech</creator><creator>Skawiński, Dariusz</creator><creator>Zmelonek-Znamirowska, Anna</creator><creator>Kedzia, Witold</creator><creator>Karowicz-Bilińska, Agata</creator><creator>Spaczyński, Robert</creator><creator>Spaczyński, Marek</creator><general>Wydawnictwo Via Medica</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20121101</creationdate><title>Results of pap smears and immunocytochemical detection of the p16 and Ki67 proteins in women with cervical intraepithelial neoplasia and cervical cancer</title><author>Rokita, Wojciech ; Skawiński, Dariusz ; Zmelonek-Znamirowska, Anna ; Kedzia, Witold ; Karowicz-Bilińska, Agata ; Spaczyński, Robert ; Spaczyński, Marek</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p169t-9d85ffcca883733d37eeaebb2fe05c03b2f5d737e8d74261303ef5a193a9fe393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>pol</language><creationdate>2012</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Cervical cancer</topic><topic>Cervical Intraepithelial Neoplasia - metabolism</topic><topic>Cervical Intraepithelial Neoplasia - pathology</topic><topic>Cervix Uteri - chemistry</topic><topic>Cervix Uteri - metabolism</topic><topic>Cervix Uteri - pathology</topic><topic>Cyclin-Dependent Kinase Inhibitor p16</topic><topic>Early Detection of Cancer - methods</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Ki-67 Antigen - analysis</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasm Proteins - analysis</topic><topic>Pap smear</topic><topic>Papanicolaou Test</topic><topic>Poland</topic><topic>Predictive Value of Tests</topic><topic>Proteins</topic><topic>Reproducibility of Results</topic><topic>Uterine Cervical Neoplasms - chemistry</topic><topic>Uterine Cervical Neoplasms - pathology</topic><topic>Vagina - pathology</topic><topic>Vaginal Smears</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rokita, Wojciech</creatorcontrib><creatorcontrib>Skawiński, Dariusz</creatorcontrib><creatorcontrib>Zmelonek-Znamirowska, Anna</creatorcontrib><creatorcontrib>Kedzia, Witold</creatorcontrib><creatorcontrib>Karowicz-Bilińska, Agata</creatorcontrib><creatorcontrib>Spaczyński, Robert</creatorcontrib><creatorcontrib>Spaczyński, Marek</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Ginekologia polska</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rokita, Wojciech</au><au>Skawiński, Dariusz</au><au>Zmelonek-Znamirowska, Anna</au><au>Kedzia, Witold</au><au>Karowicz-Bilińska, Agata</au><au>Spaczyński, Robert</au><au>Spaczyński, Marek</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Results of pap smears and immunocytochemical detection of the p16 and Ki67 proteins in women with cervical intraepithelial neoplasia and cervical cancer</atitle><jtitle>Ginekologia polska</jtitle><addtitle>Ginekol Pol</addtitle><date>2012-11-01</date><risdate>2012</risdate><volume>83</volume><issue>11</issue><spage>822</spage><epage>826</epage><pages>822-826</pages><issn>0017-0011</issn><eissn>2543-6767</eissn><abstract>The aim of the study was to assess the diagnostic value of pap smears and detection of the p16 and Ki67 proteins in women with cervical intraepithelial neoplasia (CIN).
630 women, aged between 25 and 65, with abnormal pap smears were included into the study All patients had a control pap smear and in each case punch biopsy with endocervical curettage were performed under the control of a colposcope. The presence of p16 and Ki67 proteins was detected using the CINtecPlus test. The results of the research were statistically assessed.
Abnormal pap smears were found in 82.5% (520/630) of the studied women. In 40% (252/630) of the cases the LSIL changes were found. The recognition of ASC-US concerned 35.2% (222/630) of the patients, and pap smears with the HSIL result were found in 7.3% (46/630) of the women. In 17.5% (110/630) of the patients the result of the cytological examination was normal. Abnormal results of the pap smears were found significantly statistically more frequently (p<0,0001) in women with cervical intraepithelial neoplasia (CIN). The results of the CINtecPlus test were positive in 68,4% of women with CIN and in 33,3% of patients with normal cervix. In the group of women with precancerous lesions (HGSIL/CIN2+) the diagnostic accuracy of the pap smear was 41% for the cytological results ASC-US, 56% LSIL and 73% for detection of HSIL. Immunocytochemical detection of p16 and Ki67 proteins gained the highest accuracy (78%) in recognition of cervical precancerous lesions.
1. ASC-US and LSIL cytological recognition has low accuracy in the diagnosis of CIN2+ cervical changes. 2. Cytological recognition of HSIL has the highest accuracy in the diagnosis of CIN2+ changes. 3. Immunocytochemical detection of p16 and Ki67 proteins is more accurate in recognizing precancerous states and cervical cancer than cytological examination. 4. Immunocytochemical detection of the p16 and Ki67 proteins can be used to triage patients with atypical squamous cells of undetermined significance and low grade squamous intraepithelial lesions.</abstract><cop>Poland</cop><pub>Wydawnictwo Via Medica</pub><pmid>23379189</pmid><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Biomarkers, Tumor - analysis Cervical cancer Cervical Intraepithelial Neoplasia - metabolism Cervical Intraepithelial Neoplasia - pathology Cervix Uteri - chemistry Cervix Uteri - metabolism Cervix Uteri - pathology Cyclin-Dependent Kinase Inhibitor p16 Early Detection of Cancer - methods Female Humans Immunohistochemistry Ki-67 Antigen - analysis Male Middle Aged Neoplasm Proteins - analysis Pap smear Papanicolaou Test Poland Predictive Value of Tests Proteins Reproducibility of Results Uterine Cervical Neoplasms - chemistry Uterine Cervical Neoplasms - pathology Vagina - pathology Vaginal Smears |
| title | Results of pap smears and immunocytochemical detection of the p16 and Ki67 proteins in women with cervical intraepithelial neoplasia and cervical cancer |
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