High-resolution melting analysis allowed fast and accurate closed-tube genotyping of Fusarium oxysporum formae speciales complex

Abstract The fungus Fusarium oxysporum is a highly complex species composed by many strains put together into groups called formae speciales. As it is difficult and laborious to discriminate Fusarium formae specials via biochemical or phenotypic methods, it is very important to develop novel, rapid,...

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Veröffentlicht in:	FEMS microbiology letters 2012-09, Vol.334 (1), p.16-21
Hauptverfasser:	Ganopoulos, Ioannis, Madesis, Panagiotis, Zambounis, Antonios, Tsaftaris, Athanasios
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Chemistry Techniques, Analytical - methods Contamination Data acquisition DNA Primers - chemistry DNA Primers - genetics DNA, Fungal - chemistry DNA, Fungal - genetics DNA, Intergenic - chemistry DNA, Intergenic - genetics formae speciales complex Fundamental and applied biological sciences. Psychology Fungal plant pathogens Fungi Fusarium Fusarium - chemistry Fusarium - classification Fusarium - genetics Fusarium - isolation & purification Fusarium oxysporum Genetic analysis Genotype Genotyping High resolution HRM Identification methods internal transcribed spacer Melting Melting curve Microbiology Miscellaneous Mycology Nucleotide sequence Nucleotides Phytopathology. Animal pests. Plant and forest protection Plant Diseases - microbiology Primers Real-Time Polymerase Chain Reaction Risk reduction Sensitivity and Specificity Spacer Transition Temperature
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