Activin A is essential for Feeder-free culture of human induced pluripotent stem cells

Feeder‐free culture of human induced pluripotent stem (hiPS) cells is necessary for their clinical application to avoid adverse effects of foreign proteins. hiPS cells were cultured with combinations of activin (A), CHIR99021 (C), basic fibroblast growth factor (F), and leukemia inhibitory factor (L...

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Veröffentlicht in:	Journal of cellular biochemistry 2013-03, Vol.114 (3), p.584-588
Hauptverfasser:	Tomizawa, Minoru, Shinozaki, Fuminobu, Sugiyama, Takao, Yamamoto, Shigenori, Sueishi, Makoto, Yoshida, Takanobu
Format:	Artikel
Sprache:	eng
Schlagworte:	Activins - antagonists & inhibitors Activins - pharmacology Alkaline Phosphatase - analysis ALKALINE PHOSPHATASE STAINING Animals Antigens, Surface - analysis BASIC FIBROBLAST GROWTH FACTOR Benzamides - pharmacology Cell Culture Techniques CHIR99012 Collagen Dioxoles - pharmacology Drug Combinations Feeder Cells Fibroblast Growth Factor 2 - pharmacology Homeodomain Proteins - analysis Humans Induced Pluripotent Stem Cells - metabolism Laminin LEUKEMIA INHIBITORY FACTOR Leukemia Inhibitory Factor - pharmacology Mice Nanog Homeobox Protein Octamer Transcription Factor-3 - analysis PASSAGE Proteoglycans - analysis Pyridines - pharmacology Pyrimidines - pharmacology SB431542 Stage-Specific Embryonic Antigens - analysis
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