Analysis of Antibiotic Resistance in Bacteria Isolated from the Surface Microlayer and Underlying Water of an Estuarine Environment
We compared the prevalence of cultivable antibiotic-resistant bacteria and resistance genes in the surface microlayer (SML) and underlying waters (UW) of an estuary. Prevalence of resistant bacteria was determined in antibiotic-supplemented agar. Bacterial isolates from the UW ( n =91) and SML ( n =...
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| Veröffentlicht in: | Microbial drug resistance (Larchmont, N.Y.) N.Y.), 2013-02, Vol.19 (1), p.64-71 |
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| container_title | Microbial drug resistance (Larchmont, N.Y.) |
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| creator | Azevedo, Juliana S.N. Araújo, Susana Oliveira, Cláudia S. Correia, António Henriques, Isabel |
| description | We compared the prevalence of cultivable antibiotic-resistant bacteria and resistance genes in the surface microlayer (SML) and underlying waters (UW) of an estuary. Prevalence of resistant bacteria was determined in antibiotic-supplemented agar. Bacterial isolates from the UW (
n
=91) and SML (
n
=80), selected in media without antibiotic, were characterized concerning susceptibility against nine antibiotics. The presence of genes
bla
TEM
,
bla
OXA-B
,
bla
SHV
,
bla
IMP
,
tet(A)
,
tet(B)
,
tet(E)
,
tet(M)
,
cat
,
sul1
,
sul2
,
sul3
,
aadA
,
IntI1
,
IntI2
, and
IntI3
was assessed by PCR. The variable regions of integrons were sequenced. Ampicillin- and streptomycin-resistant bacteria were significantly more prevalent in SML. Resistance levels among the bacterial collections were generally low, preventing detection of significant differences between SML and UW. The
tet(E)
gene was detected in two
Aeromonas
isolates and
tet(M)
was detected in a
Pseudomonas
isolate. Gene
sul1
was amplified from three
Aeromonas
isolates. Prevalence of
intI
genes was 2.11%. Cassette arrays contained genes encoding resistance to aminoglycosides and chloramphenicol. A higher prevalence of antibiotic-resistant bacteria in the SML, although only detectable when bacteria were selected in antibiotic-supplemented agar, suggests that SML conditions select for antibiotic resistance. Results also showed that antibiotic resistance was uncommon among estuarine bacteria and the resistance mechanisms are probably predominantly intrinsic. |
| doi_str_mv | 10.1089/mdr.2012.0084 |
| format | Article |
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n
=91) and SML (
n
=80), selected in media without antibiotic, were characterized concerning susceptibility against nine antibiotics. The presence of genes
bla
TEM
,
bla
OXA-B
,
bla
SHV
,
bla
IMP
,
tet(A)
,
tet(B)
,
tet(E)
,
tet(M)
,
cat
,
sul1
,
sul2
,
sul3
,
aadA
,
IntI1
,
IntI2
, and
IntI3
was assessed by PCR. The variable regions of integrons were sequenced. Ampicillin- and streptomycin-resistant bacteria were significantly more prevalent in SML. Resistance levels among the bacterial collections were generally low, preventing detection of significant differences between SML and UW. The
tet(E)
gene was detected in two
Aeromonas
isolates and
tet(M)
was detected in a
Pseudomonas
isolate. Gene
sul1
was amplified from three
Aeromonas
isolates. Prevalence of
intI
genes was 2.11%. Cassette arrays contained genes encoding resistance to aminoglycosides and chloramphenicol. A higher prevalence of antibiotic-resistant bacteria in the SML, although only detectable when bacteria were selected in antibiotic-supplemented agar, suggests that SML conditions select for antibiotic resistance. Results also showed that antibiotic resistance was uncommon among estuarine bacteria and the resistance mechanisms are probably predominantly intrinsic.</description><identifier>ISSN: 1076-6294</identifier><identifier>EISSN: 1931-8448</identifier><identifier>DOI: 10.1089/mdr.2012.0084</identifier><identifier>PMID: 23067198</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Antibiotic resistance ; Antibiotics ; Bacteria ; Bacteria - drug effects ; Bacteria - enzymology ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacteriology ; Drug resistance ; Drug Resistance, Multiple, Bacterial ; Environment ; Estuaries ; Estuarine environments ; Integrases - genetics ; Microbial Sensitivity Tests - methods ; Veterinary Microbiology ; Water ; Water Microbiology</subject><ispartof>Microbial drug resistance (Larchmont, N.Y.), 2013-02, Vol.19 (1), p.64-71</ispartof><rights>2013, Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2013, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-5984b545ef7fe9c0a325c3ea40d60932ae172af285a472b97bac645c488d515a3</citedby><cites>FETCH-LOGICAL-c365t-5984b545ef7fe9c0a325c3ea40d60932ae172af285a472b97bac645c488d515a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23067198$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Azevedo, Juliana S.N.</creatorcontrib><creatorcontrib>Araújo, Susana</creatorcontrib><creatorcontrib>Oliveira, Cláudia S.</creatorcontrib><creatorcontrib>Correia, António</creatorcontrib><creatorcontrib>Henriques, Isabel</creatorcontrib><title>Analysis of Antibiotic Resistance in Bacteria Isolated from the Surface Microlayer and Underlying Water of an Estuarine Environment</title><title>Microbial drug resistance (Larchmont, N.Y.)</title><addtitle>Microb Drug Resist</addtitle><description>We compared the prevalence of cultivable antibiotic-resistant bacteria and resistance genes in the surface microlayer (SML) and underlying waters (UW) of an estuary. Prevalence of resistant bacteria was determined in antibiotic-supplemented agar. Bacterial isolates from the UW (
n
=91) and SML (
n
=80), selected in media without antibiotic, were characterized concerning susceptibility against nine antibiotics. The presence of genes
bla
TEM
,
bla
OXA-B
,
bla
SHV
,
bla
IMP
,
tet(A)
,
tet(B)
,
tet(E)
,
tet(M)
,
cat
,
sul1
,
sul2
,
sul3
,
aadA
,
IntI1
,
IntI2
, and
IntI3
was assessed by PCR. The variable regions of integrons were sequenced. Ampicillin- and streptomycin-resistant bacteria were significantly more prevalent in SML. Resistance levels among the bacterial collections were generally low, preventing detection of significant differences between SML and UW. The
tet(E)
gene was detected in two
Aeromonas
isolates and
tet(M)
was detected in a
Pseudomonas
isolate. Gene
sul1
was amplified from three
Aeromonas
isolates. Prevalence of
intI
genes was 2.11%. Cassette arrays contained genes encoding resistance to aminoglycosides and chloramphenicol. A higher prevalence of antibiotic-resistant bacteria in the SML, although only detectable when bacteria were selected in antibiotic-supplemented agar, suggests that SML conditions select for antibiotic resistance. Results also showed that antibiotic resistance was uncommon among estuarine bacteria and the resistance mechanisms are probably predominantly intrinsic.</description><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - enzymology</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteriology</subject><subject>Drug resistance</subject><subject>Drug Resistance, Multiple, Bacterial</subject><subject>Environment</subject><subject>Estuaries</subject><subject>Estuarine environments</subject><subject>Integrases - genetics</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Veterinary Microbiology</subject><subject>Water</subject><subject>Water Microbiology</subject><issn>1076-6294</issn><issn>1931-8448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0U2L1TAUBuAgijOOLt1KwI2bXvPdZHkdrjowIqiDy3KanmqGNh2TVLhr_7gpd3ThxlXCyZMXkpeQ55ztOLPu9TyknWBc7Biz6gE5507yxiplH9Y9a01jhFNn5EnOt4wxzY18TM6EZKblzp6TX_sI0zGHTJeR7mMJfVhK8PQT1lmB6JGGSN-AL5gC0Ku8TFBwoGNaZlq-I_28phGq-hB8qmdHTBTiQG_igGk6hviNfq0X0hYPkR5yWSGFiPQQf4a0xBljeUoejTBlfHa_XpCbt4cvl--b64_vri73142XRpdGO6t6rTSO7YjOM5BCe4mg2GCYkwKQtwJGYTWoVvSu7cEbpb2ydtBcg7wgr065d2n5sWIu3Ryyx2mCiMuaOy5aaZky0lX68h96u6ypflVV0hipLXOiquak6tNzTjh2dynMkI4dZ93WTlfb6bZ2uq2d6l_cp679jMNf_aeOCuQJbGOIcQrYYyr_if0Nb_2cvw</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Azevedo, Juliana S.N.</creator><creator>Araújo, Susana</creator><creator>Oliveira, Cláudia S.</creator><creator>Correia, António</creator><creator>Henriques, Isabel</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20130201</creationdate><title>Analysis of Antibiotic Resistance in Bacteria Isolated from the Surface Microlayer and Underlying Water of an Estuarine Environment</title><author>Azevedo, Juliana S.N. ; Araújo, Susana ; Oliveira, Cláudia S. ; Correia, António ; Henriques, Isabel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-5984b545ef7fe9c0a325c3ea40d60932ae172af285a472b97bac645c488d515a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - enzymology</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteriology</topic><topic>Drug resistance</topic><topic>Drug Resistance, Multiple, Bacterial</topic><topic>Environment</topic><topic>Estuaries</topic><topic>Estuarine environments</topic><topic>Integrases - genetics</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Veterinary Microbiology</topic><topic>Water</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azevedo, Juliana S.N.</creatorcontrib><creatorcontrib>Araújo, Susana</creatorcontrib><creatorcontrib>Oliveira, Cláudia S.</creatorcontrib><creatorcontrib>Correia, António</creatorcontrib><creatorcontrib>Henriques, Isabel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Public Health Database (Proquest)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Azevedo, Juliana S.N.</au><au>Araújo, Susana</au><au>Oliveira, Cláudia S.</au><au>Correia, António</au><au>Henriques, Isabel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of Antibiotic Resistance in Bacteria Isolated from the Surface Microlayer and Underlying Water of an Estuarine Environment</atitle><jtitle>Microbial drug resistance (Larchmont, N.Y.)</jtitle><addtitle>Microb Drug Resist</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>19</volume><issue>1</issue><spage>64</spage><epage>71</epage><pages>64-71</pages><issn>1076-6294</issn><eissn>1931-8448</eissn><abstract>We compared the prevalence of cultivable antibiotic-resistant bacteria and resistance genes in the surface microlayer (SML) and underlying waters (UW) of an estuary. Prevalence of resistant bacteria was determined in antibiotic-supplemented agar. Bacterial isolates from the UW (
n
=91) and SML (
n
=80), selected in media without antibiotic, were characterized concerning susceptibility against nine antibiotics. The presence of genes
bla
TEM
,
bla
OXA-B
,
bla
SHV
,
bla
IMP
,
tet(A)
,
tet(B)
,
tet(E)
,
tet(M)
,
cat
,
sul1
,
sul2
,
sul3
,
aadA
,
IntI1
,
IntI2
, and
IntI3
was assessed by PCR. The variable regions of integrons were sequenced. Ampicillin- and streptomycin-resistant bacteria were significantly more prevalent in SML. Resistance levels among the bacterial collections were generally low, preventing detection of significant differences between SML and UW. The
tet(E)
gene was detected in two
Aeromonas
isolates and
tet(M)
was detected in a
Pseudomonas
isolate. Gene
sul1
was amplified from three
Aeromonas
isolates. Prevalence of
intI
genes was 2.11%. Cassette arrays contained genes encoding resistance to aminoglycosides and chloramphenicol. A higher prevalence of antibiotic-resistant bacteria in the SML, although only detectable when bacteria were selected in antibiotic-supplemented agar, suggests that SML conditions select for antibiotic resistance. Results also showed that antibiotic resistance was uncommon among estuarine bacteria and the resistance mechanisms are probably predominantly intrinsic.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>23067198</pmid><doi>10.1089/mdr.2012.0084</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1076-6294 |
| ispartof | Microbial drug resistance (Larchmont, N.Y.), 2013-02, Vol.19 (1), p.64-71 |
| issn | 1076-6294 1931-8448 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1273804639 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Antibiotic resistance Antibiotics Bacteria Bacteria - drug effects Bacteria - enzymology Bacteria - genetics Bacteria - isolation & purification Bacteriology Drug resistance Drug Resistance, Multiple, Bacterial Environment Estuaries Estuarine environments Integrases - genetics Microbial Sensitivity Tests - methods Veterinary Microbiology Water Water Microbiology |
| title | Analysis of Antibiotic Resistance in Bacteria Isolated from the Surface Microlayer and Underlying Water of an Estuarine Environment |
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