Downregulation of cornulin in esophageal squamous cell carcinoma
Early events in the development of esophageal squamous cell carcinoma (ESCC) are poorly understood and many of the key molecules involved have not yet been identified. We previously used isobaric tags for a relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach to identify...
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| Veröffentlicht in: | Acta histochemica 2013-03, Vol.115 (2), p.89-99 |
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| creator | Pawar, Harsh Maharudraiah, Jagadeesha Kashyap, Manoj Kumar Sharma, Jyoti Srikanth, Srinivas Manda Choudhary, Robin Chavan, Sandip Sathe, Gajanan Manju, Hosuru Chikkalingaiah Kumar, Kariyanakatte Veeraiah Veerendra Vijayakumar, Manavalan Sirdeshmukh, Ravi Harsha, Hindahally Chandregowda Prasad, Thottethodi Subrahmanya Keshava Pandey, Akhilesh Kumar, Rekha Vijay |
| description | Early events in the development of esophageal squamous cell carcinoma (ESCC) are poorly understood and many of the key molecules involved have not yet been identified. We previously used isobaric tags for a relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach to identify differentially expressed proteins in ESCC tissue as compared to the adjacent normal mucosa. Cornulin was identified as one of the major downregulated molecules in ESCC. Cornulin is a member of the S100 fused-type protein family, which has an EF-hand calcium binding motif and multiple tandem repeats of specific peptide motifs. Cornulin was 5-fold downregulated in ESCC as compared to normal epithelium mirroring our previous findings in a gene expression study of ESCC. In the present study, we performed immunohistochemical validation of cornulin (CRNN) in a larger set of patients with ESCC. Downregulation of cornulin was observed in 89% (n=239) of 266 different ESCC tissues arrayed on tissue microarrays (TMAs). Expression of cornulin was observed in the prickle and functional cell layers of normal esophageal mucosa, localized predominantly in the cytoplasm and perinuclear region. The large majority of ESCC cases had little or no expression of cornulin in the carcinoma or stroma. These findings suggest that cornulin is an important molecule in normal esophageal pathology and is likely lost during the conversion of normal to neoplastic epithelium. |
| doi_str_mv | 10.1016/j.acthis.2012.04.003 |
| format | Article |
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We previously used isobaric tags for a relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach to identify differentially expressed proteins in ESCC tissue as compared to the adjacent normal mucosa. Cornulin was identified as one of the major downregulated molecules in ESCC. Cornulin is a member of the S100 fused-type protein family, which has an EF-hand calcium binding motif and multiple tandem repeats of specific peptide motifs. Cornulin was 5-fold downregulated in ESCC as compared to normal epithelium mirroring our previous findings in a gene expression study of ESCC. In the present study, we performed immunohistochemical validation of cornulin (CRNN) in a larger set of patients with ESCC. Downregulation of cornulin was observed in 89% (n=239) of 266 different ESCC tissues arrayed on tissue microarrays (TMAs). Expression of cornulin was observed in the prickle and functional cell layers of normal esophageal mucosa, localized predominantly in the cytoplasm and perinuclear region. The large majority of ESCC cases had little or no expression of cornulin in the carcinoma or stroma. These findings suggest that cornulin is an important molecule in normal esophageal pathology and is likely lost during the conversion of normal to neoplastic epithelium.</description><identifier>ISSN: 0065-1281</identifier><identifier>EISSN: 1618-0372</identifier><identifier>DOI: 10.1016/j.acthis.2012.04.003</identifier><identifier>PMID: 22560086</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Adult ; Aged ; Amino Acid Sequence ; C1Orf10 ; Carcinoma, Squamous Cell - metabolism ; Cell Differentiation ; Cell Line, Tumor ; Cell Nucleus - metabolism ; Cytoplasm - metabolism ; Down-Regulation ; Epidermal differentiation complex ; Esophageal Neoplasms - metabolism ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Immunohistochemistry ; Loss of heterozygosity ; Male ; Mass Spectrometry ; Membrane Proteins - metabolism ; Middle Aged ; Molecular Sequence Data ; Neoplasm Proteins - metabolism ; Oligonucleotide Array Sequence Analysis ; S100 gene family ; S100 Proteins - metabolism ; Squamous epithelial-induced stress protein of 53 kDa ; Stress response protein</subject><ispartof>Acta histochemica, 2013-03, Vol.115 (2), p.89-99</ispartof><rights>2012 Elsevier GmbH</rights><rights>Copyright © 2012 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-9a5cc528bcd61c39d017f2aaac22c03c7d611d4b3bc6ad48c498fa9f60e09eda3</citedby><cites>FETCH-LOGICAL-c362t-9a5cc528bcd61c39d017f2aaac22c03c7d611d4b3bc6ad48c498fa9f60e09eda3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.acthis.2012.04.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22560086$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pawar, Harsh</creatorcontrib><creatorcontrib>Maharudraiah, Jagadeesha</creatorcontrib><creatorcontrib>Kashyap, Manoj Kumar</creatorcontrib><creatorcontrib>Sharma, Jyoti</creatorcontrib><creatorcontrib>Srikanth, Srinivas Manda</creatorcontrib><creatorcontrib>Choudhary, Robin</creatorcontrib><creatorcontrib>Chavan, Sandip</creatorcontrib><creatorcontrib>Sathe, Gajanan</creatorcontrib><creatorcontrib>Manju, Hosuru Chikkalingaiah</creatorcontrib><creatorcontrib>Kumar, Kariyanakatte Veeraiah Veerendra</creatorcontrib><creatorcontrib>Vijayakumar, Manavalan</creatorcontrib><creatorcontrib>Sirdeshmukh, Ravi</creatorcontrib><creatorcontrib>Harsha, Hindahally Chandregowda</creatorcontrib><creatorcontrib>Prasad, Thottethodi Subrahmanya Keshava</creatorcontrib><creatorcontrib>Pandey, Akhilesh</creatorcontrib><creatorcontrib>Kumar, Rekha Vijay</creatorcontrib><title>Downregulation of cornulin in esophageal squamous cell carcinoma</title><title>Acta histochemica</title><addtitle>Acta Histochem</addtitle><description>Early events in the development of esophageal squamous cell carcinoma (ESCC) are poorly understood and many of the key molecules involved have not yet been identified. We previously used isobaric tags for a relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach to identify differentially expressed proteins in ESCC tissue as compared to the adjacent normal mucosa. Cornulin was identified as one of the major downregulated molecules in ESCC. Cornulin is a member of the S100 fused-type protein family, which has an EF-hand calcium binding motif and multiple tandem repeats of specific peptide motifs. Cornulin was 5-fold downregulated in ESCC as compared to normal epithelium mirroring our previous findings in a gene expression study of ESCC. In the present study, we performed immunohistochemical validation of cornulin (CRNN) in a larger set of patients with ESCC. Downregulation of cornulin was observed in 89% (n=239) of 266 different ESCC tissues arrayed on tissue microarrays (TMAs). Expression of cornulin was observed in the prickle and functional cell layers of normal esophageal mucosa, localized predominantly in the cytoplasm and perinuclear region. The large majority of ESCC cases had little or no expression of cornulin in the carcinoma or stroma. These findings suggest that cornulin is an important molecule in normal esophageal pathology and is likely lost during the conversion of normal to neoplastic epithelium.</description><subject>Adult</subject><subject>Aged</subject><subject>Amino Acid Sequence</subject><subject>C1Orf10</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Cell Differentiation</subject><subject>Cell Line, Tumor</subject><subject>Cell Nucleus - metabolism</subject><subject>Cytoplasm - metabolism</subject><subject>Down-Regulation</subject><subject>Epidermal differentiation complex</subject><subject>Esophageal Neoplasms - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Loss of heterozygosity</subject><subject>Male</subject><subject>Mass Spectrometry</subject><subject>Membrane Proteins - metabolism</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>S100 gene family</subject><subject>S100 Proteins - metabolism</subject><subject>Squamous epithelial-induced stress protein of 53 kDa</subject><subject>Stress response protein</subject><issn>0065-1281</issn><issn>1618-0372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9Lw0AQxRdRbK1-A5EcvSTO7qbb5CJK_QsFL3peJpNNuyXJtruJ4rc3pdWjMDAwvDfz5sfYJYeEA1c36wSpW9mQCOAigTQBkEdszBXPYpAzcczGAGoac5HxETsLYQ0AOUhxykZCTBVApsbs7sF9td4s-xo769rIVRE53_a1baOhTHCbFS4N1lHY9ti4PkRk6joi9GRb1-A5O6mwDubi0Cfs4-nxff4SL96eX-f3i5ikEl2c45RoKrKCSsVJ5iXwWSUQkYQgkDQbxrxMC1mQwjLNKM2zCvNKgYHclCgn7Hq_d-Pdtjeh040NuyjYmiGV5mImVZbLPB2k6V5K3oXgTaU33jbovzUHvWOn13rPTu_YaUj1wG6wXR0u9EVjyj_TL6xBcLsXmOHPT2u8DmRNS6a03lCnS2f_v_ADEc2CsA</recordid><startdate>201303</startdate><enddate>201303</enddate><creator>Pawar, Harsh</creator><creator>Maharudraiah, Jagadeesha</creator><creator>Kashyap, Manoj Kumar</creator><creator>Sharma, Jyoti</creator><creator>Srikanth, Srinivas Manda</creator><creator>Choudhary, Robin</creator><creator>Chavan, Sandip</creator><creator>Sathe, Gajanan</creator><creator>Manju, Hosuru Chikkalingaiah</creator><creator>Kumar, Kariyanakatte Veeraiah Veerendra</creator><creator>Vijayakumar, Manavalan</creator><creator>Sirdeshmukh, Ravi</creator><creator>Harsha, Hindahally Chandregowda</creator><creator>Prasad, Thottethodi Subrahmanya Keshava</creator><creator>Pandey, Akhilesh</creator><creator>Kumar, Rekha Vijay</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201303</creationdate><title>Downregulation of cornulin in esophageal squamous cell carcinoma</title><author>Pawar, Harsh ; Maharudraiah, Jagadeesha ; Kashyap, Manoj Kumar ; Sharma, Jyoti ; Srikanth, Srinivas Manda ; Choudhary, Robin ; Chavan, Sandip ; Sathe, Gajanan ; Manju, Hosuru Chikkalingaiah ; Kumar, Kariyanakatte Veeraiah Veerendra ; Vijayakumar, Manavalan ; Sirdeshmukh, Ravi ; Harsha, Hindahally Chandregowda ; Prasad, Thottethodi Subrahmanya Keshava ; Pandey, Akhilesh ; Kumar, Rekha Vijay</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-9a5cc528bcd61c39d017f2aaac22c03c7d611d4b3bc6ad48c498fa9f60e09eda3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Amino Acid Sequence</topic><topic>C1Orf10</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Cell Differentiation</topic><topic>Cell Line, Tumor</topic><topic>Cell Nucleus - metabolism</topic><topic>Cytoplasm - metabolism</topic><topic>Down-Regulation</topic><topic>Epidermal differentiation complex</topic><topic>Esophageal Neoplasms - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Loss of heterozygosity</topic><topic>Male</topic><topic>Mass Spectrometry</topic><topic>Membrane Proteins - metabolism</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>S100 gene family</topic><topic>S100 Proteins - metabolism</topic><topic>Squamous epithelial-induced stress protein of 53 kDa</topic><topic>Stress response protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pawar, Harsh</creatorcontrib><creatorcontrib>Maharudraiah, Jagadeesha</creatorcontrib><creatorcontrib>Kashyap, Manoj Kumar</creatorcontrib><creatorcontrib>Sharma, Jyoti</creatorcontrib><creatorcontrib>Srikanth, Srinivas Manda</creatorcontrib><creatorcontrib>Choudhary, Robin</creatorcontrib><creatorcontrib>Chavan, Sandip</creatorcontrib><creatorcontrib>Sathe, Gajanan</creatorcontrib><creatorcontrib>Manju, Hosuru Chikkalingaiah</creatorcontrib><creatorcontrib>Kumar, Kariyanakatte Veeraiah Veerendra</creatorcontrib><creatorcontrib>Vijayakumar, Manavalan</creatorcontrib><creatorcontrib>Sirdeshmukh, Ravi</creatorcontrib><creatorcontrib>Harsha, Hindahally Chandregowda</creatorcontrib><creatorcontrib>Prasad, Thottethodi Subrahmanya Keshava</creatorcontrib><creatorcontrib>Pandey, Akhilesh</creatorcontrib><creatorcontrib>Kumar, Rekha Vijay</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta histochemica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pawar, Harsh</au><au>Maharudraiah, Jagadeesha</au><au>Kashyap, Manoj Kumar</au><au>Sharma, Jyoti</au><au>Srikanth, Srinivas Manda</au><au>Choudhary, Robin</au><au>Chavan, Sandip</au><au>Sathe, Gajanan</au><au>Manju, Hosuru Chikkalingaiah</au><au>Kumar, Kariyanakatte Veeraiah Veerendra</au><au>Vijayakumar, Manavalan</au><au>Sirdeshmukh, Ravi</au><au>Harsha, Hindahally Chandregowda</au><au>Prasad, Thottethodi Subrahmanya Keshava</au><au>Pandey, Akhilesh</au><au>Kumar, Rekha Vijay</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Downregulation of cornulin in esophageal squamous cell carcinoma</atitle><jtitle>Acta histochemica</jtitle><addtitle>Acta Histochem</addtitle><date>2013-03</date><risdate>2013</risdate><volume>115</volume><issue>2</issue><spage>89</spage><epage>99</epage><pages>89-99</pages><issn>0065-1281</issn><eissn>1618-0372</eissn><abstract>Early events in the development of esophageal squamous cell carcinoma (ESCC) are poorly understood and many of the key molecules involved have not yet been identified. We previously used isobaric tags for a relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach to identify differentially expressed proteins in ESCC tissue as compared to the adjacent normal mucosa. Cornulin was identified as one of the major downregulated molecules in ESCC. Cornulin is a member of the S100 fused-type protein family, which has an EF-hand calcium binding motif and multiple tandem repeats of specific peptide motifs. Cornulin was 5-fold downregulated in ESCC as compared to normal epithelium mirroring our previous findings in a gene expression study of ESCC. In the present study, we performed immunohistochemical validation of cornulin (CRNN) in a larger set of patients with ESCC. Downregulation of cornulin was observed in 89% (n=239) of 266 different ESCC tissues arrayed on tissue microarrays (TMAs). Expression of cornulin was observed in the prickle and functional cell layers of normal esophageal mucosa, localized predominantly in the cytoplasm and perinuclear region. The large majority of ESCC cases had little or no expression of cornulin in the carcinoma or stroma. These findings suggest that cornulin is an important molecule in normal esophageal pathology and is likely lost during the conversion of normal to neoplastic epithelium.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>22560086</pmid><doi>10.1016/j.acthis.2012.04.003</doi><tpages>11</tpages></addata></record> |
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| subjects | Adult Aged Amino Acid Sequence C1Orf10 Carcinoma, Squamous Cell - metabolism Cell Differentiation Cell Line, Tumor Cell Nucleus - metabolism Cytoplasm - metabolism Down-Regulation Epidermal differentiation complex Esophageal Neoplasms - metabolism Female Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Loss of heterozygosity Male Mass Spectrometry Membrane Proteins - metabolism Middle Aged Molecular Sequence Data Neoplasm Proteins - metabolism Oligonucleotide Array Sequence Analysis S100 gene family S100 Proteins - metabolism Squamous epithelial-induced stress protein of 53 kDa Stress response protein |
| title | Downregulation of cornulin in esophageal squamous cell carcinoma |
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