Fast multicolor 3D imaging using aberration-corrected multifocus microscopy
An epifluorescence microscope is turned into a multifocus microscope for fast three-dimensional imaging of live biological samples. Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberr...
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| Veröffentlicht in: | Nature methods 2013-01, Vol.10 (1), p.60-63 |
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| creator | Abrahamsson, Sara Chen, Jiji Hajj, Bassam Stallinga, Sjoerd Katsov, Alexander Y Wisniewski, Jan Mizuguchi, Gaku Soule, Pierre Mueller, Florian Darzacq, Claire Dugast Darzacq, Xavier Wu, Carl Bargmann, Cornelia I Agard, David A Dahan, Maxime Gustafsson, Mats G L |
| description | An epifluorescence microscope is turned into a multifocus microscope for fast three-dimensional imaging of live biological samples.
Conventional acquisition of three-dimensional (3D) microscopy data requires sequential
z
scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image. |
| doi_str_mv | 10.1038/nmeth.2277 |
| format | Article |
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Conventional acquisition of three-dimensional (3D) microscopy data requires sequential
z
scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.</description><identifier>ISSN: 1548-7091</identifier><identifier>EISSN: 1548-7105</identifier><identifier>DOI: 10.1038/nmeth.2277</identifier><identifier>PMID: 23223154</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647/245 ; 631/1647/328 ; Animals ; Bioinformatics ; Biological Microscopy ; Biological Techniques ; Biomedical Engineering/Biotechnology ; Biophysics ; Bone Neoplasms - enzymology ; brief-communication ; Caenorhabditis elegans - cytology ; Cameras ; Cell Tracking ; Chromosomal Proteins, Non-Histone - metabolism ; DNA-Binding Proteins - metabolism ; Humans ; Imaging, Three-Dimensional - methods ; Innovations ; Life Sciences ; Methods ; Microscope and microscopy ; Microscopy ; Microscopy, Fluorescence ; Neurons - cytology ; Osteosarcoma - enzymology ; Proteomics ; RNA Polymerase II - metabolism ; Saccharomyces cerevisiae - cytology ; Saccharomyces cerevisiae Proteins - metabolism ; Scientific imaging</subject><ispartof>Nature methods, 2013-01, Vol.10 (1), p.60-63</ispartof><rights>Springer Nature Limited 2012</rights><rights>COPYRIGHT 2013 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jan 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c594t-2b68228b25878609843f876aa14420947ce6f5341ae4f12f9a8f5964bd95dda73</citedby><cites>FETCH-LOGICAL-c594t-2b68228b25878609843f876aa14420947ce6f5341ae4f12f9a8f5964bd95dda73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nmeth.2277$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nmeth.2277$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23223154$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abrahamsson, Sara</creatorcontrib><creatorcontrib>Chen, Jiji</creatorcontrib><creatorcontrib>Hajj, Bassam</creatorcontrib><creatorcontrib>Stallinga, Sjoerd</creatorcontrib><creatorcontrib>Katsov, Alexander Y</creatorcontrib><creatorcontrib>Wisniewski, Jan</creatorcontrib><creatorcontrib>Mizuguchi, Gaku</creatorcontrib><creatorcontrib>Soule, Pierre</creatorcontrib><creatorcontrib>Mueller, Florian</creatorcontrib><creatorcontrib>Darzacq, Claire Dugast</creatorcontrib><creatorcontrib>Darzacq, Xavier</creatorcontrib><creatorcontrib>Wu, Carl</creatorcontrib><creatorcontrib>Bargmann, Cornelia I</creatorcontrib><creatorcontrib>Agard, David A</creatorcontrib><creatorcontrib>Dahan, Maxime</creatorcontrib><creatorcontrib>Gustafsson, Mats G L</creatorcontrib><title>Fast multicolor 3D imaging using aberration-corrected multifocus microscopy</title><title>Nature methods</title><addtitle>Nat Methods</addtitle><addtitle>Nat Methods</addtitle><description>An epifluorescence microscope is turned into a multifocus microscope for fast three-dimensional imaging of live biological samples.
Conventional acquisition of three-dimensional (3D) microscopy data requires sequential
z
scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.</description><subject>631/1647/245</subject><subject>631/1647/328</subject><subject>Animals</subject><subject>Bioinformatics</subject><subject>Biological Microscopy</subject><subject>Biological Techniques</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biophysics</subject><subject>Bone Neoplasms - enzymology</subject><subject>brief-communication</subject><subject>Caenorhabditis elegans - cytology</subject><subject>Cameras</subject><subject>Cell Tracking</subject><subject>Chromosomal Proteins, Non-Histone - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Humans</subject><subject>Imaging, Three-Dimensional - methods</subject><subject>Innovations</subject><subject>Life Sciences</subject><subject>Methods</subject><subject>Microscope and microscopy</subject><subject>Microscopy</subject><subject>Microscopy, Fluorescence</subject><subject>Neurons - cytology</subject><subject>Osteosarcoma - enzymology</subject><subject>Proteomics</subject><subject>RNA Polymerase II - metabolism</subject><subject>Saccharomyces cerevisiae - cytology</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Scientific 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Conventional acquisition of three-dimensional (3D) microscopy data requires sequential
z
scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>23223154</pmid><doi>10.1038/nmeth.2277</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Nature; SpringerLink Journals - AutoHoldings |
| subjects | 631/1647/245 631/1647/328 Animals Bioinformatics Biological Microscopy Biological Techniques Biomedical Engineering/Biotechnology Biophysics Bone Neoplasms - enzymology brief-communication Caenorhabditis elegans - cytology Cameras Cell Tracking Chromosomal Proteins, Non-Histone - metabolism DNA-Binding Proteins - metabolism Humans Imaging, Three-Dimensional - methods Innovations Life Sciences Methods Microscope and microscopy Microscopy Microscopy, Fluorescence Neurons - cytology Osteosarcoma - enzymology Proteomics RNA Polymerase II - metabolism Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae Proteins - metabolism Scientific imaging |
| title | Fast multicolor 3D imaging using aberration-corrected multifocus microscopy |
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