Effect of Medicaments Used in Endodontic Regeneration Technique on the Chemical Structure of Human Immature Radicular Dentin: An In Vitro Study
Abstract Introduction The aim of this study was to investigate the effect of 3 intracanal medicaments used in pulp regeneration on the chemical structure of radicular dentin. Methods Human immature radicular dentin specimens were exposed to triple antibiotic paste (tripaste), double antibiotic paste...
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| Veröffentlicht in: | Journal of endodontics 2013-02, Vol.39 (2), p.269-273 |
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| creator | Yassen, Ghaeth H., BDS, MSD Chu, Tien-Min G., DDS, PhD Eckert, George, MAS Platt, Jeffrey A., DDS, MS |
| description | Abstract Introduction The aim of this study was to investigate the effect of 3 intracanal medicaments used in pulp regeneration on the chemical structure of radicular dentin. Methods Human immature radicular dentin specimens were exposed to triple antibiotic paste (tripaste), double antibiotic paste (bipaste), calcium hydroxide [(Ca(OH)2 ] paste, or deionized water (control) for 1, 2, or 4 weeks. After each time point, specimens were examined with attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to analyze the relative loss of organic and inorganic components by using phosphate/amide I ratios. Data were analyzed by using analysis of variance followed by post hoc comparisons. Results Phosphate/amide ratios were significantly different between the 4 groups at all time points ( P < .0001): Ca(OH)2 -treated dentin > untreated control dentin > bipaste-treated dentin > tripaste-treated dentin. For bipaste groups, 4-week treated dentin had significantly lower phosphate/amide I ratios than 1- and 2-week treated dentin ( P < .05); however, phosphate/amide I ratios of 1- and 2-week treated dentin did not have a significant difference. For tripaste groups, 4-week treated dentin had a significantly higher phosphate/amide I ratio than 1- and 2-week treated dentin ( P < .001), and phosphate/amide I ratio of 1-week treated dentin was significantly higher than that of 2-week treated dentin ( P = .04). No significant time effect for Ca(OH)2 -treated dentin or untreated control dentin was found ( P > .05). Conclusions The results suggested a superficial collagen degradation or demineralization of radicular dentin caused by Ca(OH)2 or antibiotic pastes, respectively, after 1, 2, or 4 weeks of exposure. |
| doi_str_mv | 10.1016/j.joen.2012.09.020 |
| format | Article |
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Methods Human immature radicular dentin specimens were exposed to triple antibiotic paste (tripaste), double antibiotic paste (bipaste), calcium hydroxide [(Ca(OH)2 ] paste, or deionized water (control) for 1, 2, or 4 weeks. After each time point, specimens were examined with attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to analyze the relative loss of organic and inorganic components by using phosphate/amide I ratios. Data were analyzed by using analysis of variance followed by post hoc comparisons. Results Phosphate/amide ratios were significantly different between the 4 groups at all time points ( P < .0001): Ca(OH)2 -treated dentin > untreated control dentin > bipaste-treated dentin > tripaste-treated dentin. For bipaste groups, 4-week treated dentin had significantly lower phosphate/amide I ratios than 1- and 2-week treated dentin ( P < .05); however, phosphate/amide I ratios of 1- and 2-week treated dentin did not have a significant difference. For tripaste groups, 4-week treated dentin had a significantly higher phosphate/amide I ratio than 1- and 2-week treated dentin ( P < .001), and phosphate/amide I ratio of 1-week treated dentin was significantly higher than that of 2-week treated dentin ( P = .04). No significant time effect for Ca(OH)2 -treated dentin or untreated control dentin was found ( P > .05). Conclusions The results suggested a superficial collagen degradation or demineralization of radicular dentin caused by Ca(OH)2 or antibiotic pastes, respectively, after 1, 2, or 4 weeks of exposure.</description><identifier>ISSN: 0099-2399</identifier><identifier>EISSN: 1878-3554</identifier><identifier>DOI: 10.1016/j.joen.2012.09.020</identifier><identifier>PMID: 23321244</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amides - analysis ; Anti-Bacterial Agents - administration & dosage ; Anti-Bacterial Agents - pharmacology ; Apatites - analysis ; Calcium hydroxide ; Calcium Hydroxide - pharmacology ; Carbonates - analysis ; Ciprofloxacin - administration & dosage ; Ciprofloxacin - pharmacology ; Collagen - analysis ; Dentin - chemistry ; Dentin - drug effects ; Dentistry ; double antibiotic paste ; Drug Combinations ; Endocrinology & Metabolism ; endodontic regeneration ; Fourier transform infrared spectroscopy ; Humans ; Hydrogen-Ion Concentration ; Metronidazole - administration & dosage ; Metronidazole - pharmacology ; Microscopy, Electron, Scanning ; Minocycline - administration & dosage ; Minocycline - pharmacology ; Phosphates - analysis ; Regeneration ; Root Canal Irrigants - pharmacology ; Root Canal Therapy - methods ; Spectroscopy, Fourier Transform Infrared ; Temperature ; Time Factors ; Tooth Root - chemistry ; Tooth Root - drug effects ; triple antibiotic paste ; Water</subject><ispartof>Journal of endodontics, 2013-02, Vol.39 (2), p.269-273</ispartof><rights>American Association of Endodontists</rights><rights>2013 American Association of Endodontists</rights><rights>Copyright © 2013 American Association of Endodontists. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-fd79e9f773877a5a976587d3f9afe7f39fa91e74ad70bc92cf9b5bf77fe740df3</citedby><cites>FETCH-LOGICAL-c477t-fd79e9f773877a5a976587d3f9afe7f39fa91e74ad70bc92cf9b5bf77fe740df3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.joen.2012.09.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23321244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yassen, Ghaeth H., BDS, MSD</creatorcontrib><creatorcontrib>Chu, Tien-Min G., DDS, PhD</creatorcontrib><creatorcontrib>Eckert, George, MAS</creatorcontrib><creatorcontrib>Platt, Jeffrey A., DDS, MS</creatorcontrib><title>Effect of Medicaments Used in Endodontic Regeneration Technique on the Chemical Structure of Human Immature Radicular Dentin: An In Vitro Study</title><title>Journal of endodontics</title><addtitle>J Endod</addtitle><description>Abstract Introduction The aim of this study was to investigate the effect of 3 intracanal medicaments used in pulp regeneration on the chemical structure of radicular dentin. Methods Human immature radicular dentin specimens were exposed to triple antibiotic paste (tripaste), double antibiotic paste (bipaste), calcium hydroxide [(Ca(OH)2 ] paste, or deionized water (control) for 1, 2, or 4 weeks. After each time point, specimens were examined with attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to analyze the relative loss of organic and inorganic components by using phosphate/amide I ratios. Data were analyzed by using analysis of variance followed by post hoc comparisons. Results Phosphate/amide ratios were significantly different between the 4 groups at all time points ( P < .0001): Ca(OH)2 -treated dentin > untreated control dentin > bipaste-treated dentin > tripaste-treated dentin. For bipaste groups, 4-week treated dentin had significantly lower phosphate/amide I ratios than 1- and 2-week treated dentin ( P < .05); however, phosphate/amide I ratios of 1- and 2-week treated dentin did not have a significant difference. For tripaste groups, 4-week treated dentin had a significantly higher phosphate/amide I ratio than 1- and 2-week treated dentin ( P < .001), and phosphate/amide I ratio of 1-week treated dentin was significantly higher than that of 2-week treated dentin ( P = .04). No significant time effect for Ca(OH)2 -treated dentin or untreated control dentin was found ( P > .05). Conclusions The results suggested a superficial collagen degradation or demineralization of radicular dentin caused by Ca(OH)2 or antibiotic pastes, respectively, after 1, 2, or 4 weeks of exposure.</description><subject>Amides - analysis</subject><subject>Anti-Bacterial Agents - administration & dosage</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Apatites - analysis</subject><subject>Calcium hydroxide</subject><subject>Calcium Hydroxide - pharmacology</subject><subject>Carbonates - analysis</subject><subject>Ciprofloxacin - administration & dosage</subject><subject>Ciprofloxacin - pharmacology</subject><subject>Collagen - analysis</subject><subject>Dentin - chemistry</subject><subject>Dentin - drug effects</subject><subject>Dentistry</subject><subject>double antibiotic paste</subject><subject>Drug Combinations</subject><subject>Endocrinology & Metabolism</subject><subject>endodontic regeneration</subject><subject>Fourier transform infrared spectroscopy</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Metronidazole - administration & dosage</subject><subject>Metronidazole - pharmacology</subject><subject>Microscopy, Electron, Scanning</subject><subject>Minocycline - administration & dosage</subject><subject>Minocycline - pharmacology</subject><subject>Phosphates - analysis</subject><subject>Regeneration</subject><subject>Root Canal Irrigants - pharmacology</subject><subject>Root Canal Therapy - methods</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Temperature</subject><subject>Time Factors</subject><subject>Tooth Root - chemistry</subject><subject>Tooth Root - drug effects</subject><subject>triple antibiotic paste</subject><subject>Water</subject><issn>0099-2399</issn><issn>1878-3554</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kktuFDEQhi0EIpPABVggL9l040f3uI0QUjQMJFIQUh5sLY9dZjx024ndjTS3yBE4CyfDzQQWLFj5Uf9fpaqvEHpBSU0JXb7e1bsIoWaEsprImjDyCC1oJ7qKt23zGC0IkbJiXMojdJzzjhAqOBdP0RHjnFHWNAt0v3YOzIijw5_AeqMHCGPGNxks9gGvg402htEbfAlfIUDSo48BX4PZBn83AS6PcQt4tYWhuHt8NabJjFOCOeXZNOiAz4dB__651KXC1OuE35cqPrzBpyUafv744scUi3Wy-2foidN9hucP5wm6-bC-Xp1VF58_nq9OLyrTCDFWzgoJ0gnBOyF0q6VYtp2w3EntQDgunZYURKOtIBsjmXFy026KvkQbYh0_Qa8OeW9TLH3kUQ0-G-h7HSBOWVEmeNtxJpZFyg5Sk2LOCZy6TX7Qaa8oUTMJtVMzCTWTUESqQqKYXj7knzYD2L-WP6MvgrcHAZQuv3tIKhsPwRQKqRBRNvr_53_3j930PswIvsEe8i5OKZT5Kapy8aireRfmVaCs3CgR_Bc_vrHO</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Yassen, Ghaeth H., BDS, MSD</creator><creator>Chu, Tien-Min G., DDS, PhD</creator><creator>Eckert, George, MAS</creator><creator>Platt, Jeffrey A., DDS, MS</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130201</creationdate><title>Effect of Medicaments Used in Endodontic Regeneration Technique on the Chemical Structure of Human Immature Radicular Dentin: An In Vitro Study</title><author>Yassen, Ghaeth H., BDS, MSD ; Chu, Tien-Min G., DDS, PhD ; Eckert, George, MAS ; Platt, Jeffrey A., DDS, MS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-fd79e9f773877a5a976587d3f9afe7f39fa91e74ad70bc92cf9b5bf77fe740df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Amides - analysis</topic><topic>Anti-Bacterial Agents - administration & dosage</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Apatites - analysis</topic><topic>Calcium hydroxide</topic><topic>Calcium Hydroxide - pharmacology</topic><topic>Carbonates - analysis</topic><topic>Ciprofloxacin - administration & dosage</topic><topic>Ciprofloxacin - pharmacology</topic><topic>Collagen - analysis</topic><topic>Dentin - chemistry</topic><topic>Dentin - drug effects</topic><topic>Dentistry</topic><topic>double antibiotic paste</topic><topic>Drug Combinations</topic><topic>Endocrinology & Metabolism</topic><topic>endodontic regeneration</topic><topic>Fourier transform infrared spectroscopy</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Metronidazole - administration & dosage</topic><topic>Metronidazole - pharmacology</topic><topic>Microscopy, Electron, Scanning</topic><topic>Minocycline - administration & dosage</topic><topic>Minocycline - pharmacology</topic><topic>Phosphates - analysis</topic><topic>Regeneration</topic><topic>Root Canal Irrigants - pharmacology</topic><topic>Root Canal Therapy - methods</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Temperature</topic><topic>Time Factors</topic><topic>Tooth Root - chemistry</topic><topic>Tooth Root - drug effects</topic><topic>triple antibiotic paste</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yassen, Ghaeth H., BDS, MSD</creatorcontrib><creatorcontrib>Chu, Tien-Min G., DDS, PhD</creatorcontrib><creatorcontrib>Eckert, George, MAS</creatorcontrib><creatorcontrib>Platt, Jeffrey A., DDS, MS</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of endodontics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yassen, Ghaeth H., BDS, MSD</au><au>Chu, Tien-Min G., DDS, PhD</au><au>Eckert, George, MAS</au><au>Platt, Jeffrey A., DDS, MS</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Medicaments Used in Endodontic Regeneration Technique on the Chemical Structure of Human Immature Radicular Dentin: An In Vitro Study</atitle><jtitle>Journal of endodontics</jtitle><addtitle>J Endod</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>39</volume><issue>2</issue><spage>269</spage><epage>273</epage><pages>269-273</pages><issn>0099-2399</issn><eissn>1878-3554</eissn><abstract>Abstract Introduction The aim of this study was to investigate the effect of 3 intracanal medicaments used in pulp regeneration on the chemical structure of radicular dentin. Methods Human immature radicular dentin specimens were exposed to triple antibiotic paste (tripaste), double antibiotic paste (bipaste), calcium hydroxide [(Ca(OH)2 ] paste, or deionized water (control) for 1, 2, or 4 weeks. After each time point, specimens were examined with attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to analyze the relative loss of organic and inorganic components by using phosphate/amide I ratios. Data were analyzed by using analysis of variance followed by post hoc comparisons. Results Phosphate/amide ratios were significantly different between the 4 groups at all time points ( P < .0001): Ca(OH)2 -treated dentin > untreated control dentin > bipaste-treated dentin > tripaste-treated dentin. For bipaste groups, 4-week treated dentin had significantly lower phosphate/amide I ratios than 1- and 2-week treated dentin ( P < .05); however, phosphate/amide I ratios of 1- and 2-week treated dentin did not have a significant difference. For tripaste groups, 4-week treated dentin had a significantly higher phosphate/amide I ratio than 1- and 2-week treated dentin ( P < .001), and phosphate/amide I ratio of 1-week treated dentin was significantly higher than that of 2-week treated dentin ( P = .04). No significant time effect for Ca(OH)2 -treated dentin or untreated control dentin was found ( P > .05). Conclusions The results suggested a superficial collagen degradation or demineralization of radicular dentin caused by Ca(OH)2 or antibiotic pastes, respectively, after 1, 2, or 4 weeks of exposure.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>23321244</pmid><doi>10.1016/j.joen.2012.09.020</doi><tpages>5</tpages></addata></record> |
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| subjects | Amides - analysis Anti-Bacterial Agents - administration & dosage Anti-Bacterial Agents - pharmacology Apatites - analysis Calcium hydroxide Calcium Hydroxide - pharmacology Carbonates - analysis Ciprofloxacin - administration & dosage Ciprofloxacin - pharmacology Collagen - analysis Dentin - chemistry Dentin - drug effects Dentistry double antibiotic paste Drug Combinations Endocrinology & Metabolism endodontic regeneration Fourier transform infrared spectroscopy Humans Hydrogen-Ion Concentration Metronidazole - administration & dosage Metronidazole - pharmacology Microscopy, Electron, Scanning Minocycline - administration & dosage Minocycline - pharmacology Phosphates - analysis Regeneration Root Canal Irrigants - pharmacology Root Canal Therapy - methods Spectroscopy, Fourier Transform Infrared Temperature Time Factors Tooth Root - chemistry Tooth Root - drug effects triple antibiotic paste Water |
| title | Effect of Medicaments Used in Endodontic Regeneration Technique on the Chemical Structure of Human Immature Radicular Dentin: An In Vitro Study |
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